##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936684_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1138188 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.451837482032847 31.0 31.0 34.0 30.0 34.0 2 31.507635821147296 33.0 31.0 34.0 28.0 34.0 3 31.45636836796733 33.0 31.0 34.0 28.0 34.0 4 35.11665823220768 37.0 35.0 37.0 32.0 37.0 5 35.15183695487916 37.0 35.0 37.0 32.0 37.0 6 35.215347552425435 37.0 35.0 37.0 32.0 37.0 7 35.08280178669956 37.0 35.0 37.0 32.0 37.0 8 35.07491205319332 37.0 35.0 37.0 32.0 37.0 9 36.583428220996886 39.0 37.0 39.0 32.0 39.0 10-11 36.44979607938232 39.0 37.0 39.0 32.0 39.0 12-13 36.364649337367815 39.0 37.0 39.0 32.0 39.0 14-15 37.36467613434688 40.0 37.0 41.0 32.0 41.0 16-17 37.296930735519965 40.0 37.0 41.0 32.0 41.0 18-19 37.195627172312484 40.0 37.0 41.0 31.0 41.0 20-21 37.191868566528555 39.5 37.0 41.0 31.0 41.0 22-23 37.18634179942154 39.0 36.5 41.0 31.0 41.0 24-25 37.29880388828559 40.0 37.0 41.0 31.0 41.0 26-27 37.22760782928655 40.0 37.0 41.0 31.0 41.0 28-29 37.12064483196097 39.5 36.5 41.0 31.0 41.0 30-31 36.922269871058205 39.0 36.0 41.0 30.5 41.0 32-33 36.73620438802729 39.0 36.0 41.0 30.0 41.0 34-35 36.52295666445262 39.0 35.0 41.0 30.0 41.0 36-37 36.29905252910767 39.0 35.0 40.5 30.0 41.0 38-39 36.090134055182446 39.0 35.0 40.0 29.5 41.0 40-41 35.87634116683711 38.5 35.0 40.0 28.5 41.0 42-43 35.58949004909558 38.0 35.0 40.0 27.0 41.0 44-45 35.391500788973346 38.0 35.0 40.0 27.0 41.0 46-47 35.179696148615164 38.0 34.5 40.0 26.0 41.0 48-49 34.901435439488026 38.0 34.0 40.0 24.0 41.0 50-51 33.89696737270117 37.5 33.0 39.5 23.5 40.5 52-53 33.994336172934524 37.5 33.5 39.5 23.5 40.5 54-55 34.432928040007454 38.0 34.0 40.0 23.5 41.0 56-57 34.24203251132502 38.0 34.0 40.0 23.0 41.0 58-59 34.00863960962512 38.0 33.5 40.0 22.5 41.0 60-61 33.686300505716105 37.0 33.0 40.0 22.0 41.0 62-63 33.36843166506763 37.0 33.0 40.0 20.5 41.0 64-65 33.01609839499274 36.0 32.5 39.5 19.5 41.0 66-67 32.60744182859071 36.0 32.0 39.0 18.0 41.0 68-69 32.17343839506303 35.0 31.5 39.0 16.5 40.0 70-71 31.740998411510226 35.0 31.0 38.0 13.0 40.0 72-73 31.335116869972275 35.0 31.0 37.0 9.5 40.0 74-75 30.94049093822813 35.0 30.5 37.0 7.5 39.0 76-77 30.492028996967107 34.5 30.0 36.0 7.0 39.0 78-79 30.02005951565119 34.0 29.5 36.0 2.0 38.5 80-81 29.541089872674814 34.0 29.0 35.5 2.0 37.0 82-83 29.15037981423104 34.0 29.0 35.0 2.0 37.0 84-85 28.782413362291642 34.0 29.0 35.0 2.0 36.5 86-87 28.48310384576186 34.0 29.0 35.0 2.0 36.0 88-89 28.11619609414262 34.0 27.5 35.0 2.0 36.0 90-91 27.734680035284153 34.0 27.0 35.0 2.0 35.0 92-93 27.30624598045314 33.5 26.0 35.0 2.0 35.0 94-95 26.82843915064998 33.0 25.0 35.0 2.0 35.0 96-97 26.256355276984117 33.0 24.5 35.0 2.0 35.0 98-99 25.54059698397804 33.0 21.5 35.0 2.0 35.0 100-101 23.005768818508017 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5825128591869593 1101 2 0.4755365083236569 1101 3 0.5571150977923871 1101 4 0.5847263532781355 1101 5 0.44562971625243364 1101 6 0.5576653309101758 1101 7 0.6787053900305722 1101 8 0.6747511689372203 1101 9 0.8565559711736412 1101 10-11 0.8558308905161027 1101 12-13 0.7470881375956395 1101 14-15 0.9533684461321812 1101 16-17 1.0045389273734244 1101 18-19 1.088814263360959 1101 20-21 1.0547474215097736 1101 22-23 0.9304313133292297 1101 24-25 0.7918080810480674 1101 26-27 0.8119879161458314 1101 28-29 0.819551461107423 1101 30-31 0.6840556976582306 1101 32-33 0.6950084710274069 1101 34-35 0.8579428197109991 1101 36-37 0.8941830120494814 1101 38-39 0.8071625026526732 1101 40-41 0.8459278734165068 1101 42-43 0.9466468643590318 1101 44-45 0.9484068558009682 1101 46-47 1.1123658062986408 1101 48-49 1.1462333328097216 1101 50-51 1.1092740034824757 1101 52-53 1.2420753012575503 1101 54-55 1.3418483033139026 1101 56-57 1.3260477875239118 1101 58-59 1.06866528577914 1101 60-61 1.1254248129046474 1101 62-63 1.3449196770152767 1101 64-65 1.1365697178149858 1101 66-67 1.2499663184815262 1101 68-69 1.244283026528791 1101 70-71 1.2519033931475683 1101 72-73 1.3582063570066865 1101 74-75 1.0871354184390611 1101 76-77 1.1348116850448982 1101 78-79 0.9733801628509546 1101 80-81 1.0902034012923778 1101 82-83 1.2142780303349952 1101 84-85 0.9401742034424139 1101 86-87 1.1379729781747905 1101 88-89 1.193439656768497 1101 90-91 1.0456292307470392 1101 92-93 1.0953900789271138 1101 94-95 1.0053236390852298 1101 96-97 1.0087389142105962 1101 98-99 0.9406359246312306 1101 100-101 1.5210402222611208 1102 1 0.4031700386318633 1102 2 0.5169863774433594 1102 3 0.6785480733034284 1102 4 0.5090592477088904 1102 5 0.503590151782312 1102 6 0.4393770961341872 1102 7 0.48324543443276013 1102 8 0.535592551954629 1102 9 0.5412945832326983 1102 10-11 0.6488387885040012 1102 12-13 0.495623610786069 1102 14-15 0.861233459875173 1102 16-17 0.565132778370895 1102 18-19 0.6752659505762466 1102 20-21 0.8183703778461151 1102 22-23 0.565670974463309 1102 24-25 0.6801042883425836 1102 26-27 0.5041149976394266 1102 28-29 0.3887603749703459 1102 30-31 0.45279710122802896 1102 32-33 0.5311926384638497 1102 34-35 0.15772126048512547 1102 36-37 0.2443895522248738 1102 38-39 0.04513563097408735 1102 40-41 0.3962769251836775 1102 42-43 0.31310876001163734 1102 44-45 0.5228805679456201 1102 46-47 0.4367601320336121 1102 48-49 0.7193420052385875 1102 50-51 0.404507882846751 1102 52-53 0.26397014080215087 1102 54-55 0.5400421777998119 1102 56-57 0.5174489858860483 1102 58-59 0.3646035924884856 1102 60-61 0.24092560225173543 1102 62-63 0.47175905899829473 1102 64-65 0.5724214334874134 1102 66-67 0.47606062005051086 1102 68-69 0.4898205617332181 1102 70-71 0.5382367347620836 1102 72-73 0.42422423080601135 1102 74-75 0.319736695397129 1102 76-77 0.7078431166427244 1102 78-79 0.7018047886733321 1102 80-81 0.7998284670713005 1102 82-83 0.6009993829077729 1102 84-85 0.4460074671385428 1102 86-87 0.43272624792371417 1102 88-89 0.6916369190101541 1102 90-91 0.6817858444367317 1102 92-93 0.6309319278738599 1102 94-95 0.7496345808587428 1102 96-97 0.5873246842813522 1102 98-99 0.7547626402472076 1102 100-101 1.292688546112501 1103 1 0.36739202907727986 1103 2 0.512518258653607 1103 3 0.4577044574610696 1103 4 0.5767734110308282 1103 5 0.4489474243487308 1103 6 0.33125337077729 1103 7 0.5456580137050864 1103 8 0.33537557161323406 1103 9 0.7030562214808356 1103 10-11 0.5710939406230509 1103 12-13 0.6463690732894491 1103 14-15 0.9059193040031985 1103 16-17 0.9645512151811957 1103 18-19 0.9230642519833623 1103 20-21 0.9245000724905239 1103 22-23 0.7847058775671556 1103 24-25 0.7110419134787804 1103 26-27 0.6680385237096615 1103 28-29 0.7257920745876092 1103 30-31 0.6884440378939729 1103 32-33 0.8269123985748479 1103 34-35 0.8121900777094524 1103 36-37 0.7850456616649168 1103 38-39 0.5343105934914405 1103 40-41 0.8719825769162597 1103 42-43 0.8480861307313958 1103 44-45 0.687665263847201 1103 46-47 0.5805221800300444 1103 48-49 0.721967187995638 1103 50-51 0.5640082227515748 1103 52-53 0.6502205249411688 1103 54-55 0.8168471655539236 1103 56-57 0.8399136683776902 1103 58-59 0.6295866438094535 1103 60-61 0.7405854646135666 1103 62-63 0.696795843219256 1103 64-65 0.725040795956339 1103 66-67 0.8899403775540264 1103 68-69 0.8310247095033461 1103 70-71 0.827796534730421 1103 72-73 0.7741623029403328 1103 74-75 0.6317071655832827 1103 76-77 0.6732144975875656 1103 78-79 0.5848339925510402 1103 80-81 0.8536528619941457 1103 82-83 0.6277957566354573 1103 84-85 0.8002695477285791 1103 86-87 0.7790324602664533 1103 88-89 0.9104958166010206 1103 90-91 0.8812433935832473 1103 92-93 0.923435862388633 1103 94-95 0.8923736550138699 1103 96-97 0.978994455101688 1103 98-99 1.2610553029591784 1103 100-101 1.6454713422720424 1104 1 0.26550834926030475 1104 2 0.3099146680809852 1104 3 0.5771930986590625 1104 4 0.6027275409456507 1104 5 0.49492433804452673 1104 6 0.4809773183273194 1104 7 0.4709919709925856 1104 8 0.3557212044067555 1104 9 0.6064043028217725 1104 10-11 0.5587663552311426 1104 12-13 0.6423984558584408 1104 14-15 0.8767494466618118 1104 16-17 0.7358034722881186 1104 18-19 0.7895316787396496 1104 20-21 0.840039603768588 1104 22-23 0.7479221249555295 1104 24-25 0.8832360104941941 1104 26-27 0.5787573000031827 1104 28-29 0.48443502154204765 1104 30-31 0.5508571806376708 1104 32-33 0.5850240391016186 1104 34-35 0.6729379245948834 1104 36-37 0.7131375436430432 1104 38-39 0.4767373497806062 1104 40-41 0.6199211646999956 1104 42-43 0.8245883476594642 1104 44-45 1.0350760903973963 1104 46-47 1.201622230135456 1104 48-49 1.2549979980961226 1104 50-51 1.113093799011061 1104 52-53 1.0209412392741442 1104 54-55 1.2797044029495765 1104 56-57 1.4022639430120591 1104 58-59 1.4834790306232222 1104 60-61 1.2985562427600428 1104 62-63 1.2492658981461346 1104 64-65 1.3616888858057408 1104 66-67 1.283188909843652 1104 68-69 1.261605317764122 1104 70-71 1.088971459335962 1104 72-73 1.3129883237198996 1104 74-75 1.2534522046749537 1104 76-77 1.3409586669249443 1104 78-79 1.3466945577022535 1104 80-81 1.4945608571159603 1104 82-83 1.3099334141602483 1104 84-85 1.5077364356153602 1104 86-87 1.3379810029553134 1104 88-89 1.1723601632926446 1104 90-91 1.2928390145594477 1104 92-93 1.182423638559257 1104 94-95 1.3737188434763894 1104 96-97 1.2585984805514556 1104 98-99 1.2267257700739584 1104 100-101 1.6771783768825905 1105 1 0.33990279095269216 1105 2 0.2714578520834934 1105 3 0.42442855192279083 1105 4 0.3666815265587431 1105 5 0.3353981998574156 1105 6 0.2991129481460675 1105 7 0.30455605368976535 1105 8 0.2622909986799371 1105 9 0.5311122672736701 1105 10-11 0.3993481823427132 1105 12-13 0.43981109922999906 1105 14-15 0.5731976000318113 1105 16-17 0.4833086271018985 1105 18-19 0.3896578499514476 1105 20-21 0.3573055836912786 1105 22-23 0.5286234336643005 1105 24-25 0.310312860617195 1105 26-27 0.17975566147100608 1105 28-29 0.3532063764430333 1105 30-31 0.20214196769200043 1105 32-33 0.32929658585483423 1105 34-35 0.16479183696093003 1105 36-37 0.2886624455216378 1105 38-39 0.16562158134289007 1105 40-41 0.22261521644713156 1105 42-43 0.19129354311899505 1105 44-45 0.17680277306833148 1105 46-47 0.298479804853784 1105 48-49 0.3939302848011508 1105 50-51 0.5056408062553217 1105 52-53 0.6986669396725631 1105 54-55 0.6157093481836498 1105 56-57 0.5100556504303313 1105 58-59 0.33259646723065117 1105 60-61 0.39244159631464015 1105 62-63 0.5800948599433511 1105 64-65 0.7366587190311051 1105 66-67 0.644032224674703 1105 68-69 0.509411081840895 1105 70-71 0.7264705596251382 1105 72-73 0.4978524356312235 1105 74-75 0.530736729225417 1105 76-77 0.6733118447035693 1105 78-79 0.6993812000661457 1105 80-81 0.8485261473309187 1105 82-83 0.6872911857564858 1105 84-85 0.7830521811898414 1105 86-87 0.777054703031947 1105 88-89 0.6689645488007372 1105 90-91 0.7541556831313869 1105 92-93 0.8710771207576862 1105 94-95 0.9847833813214031 1105 96-97 1.0362217197644519 1105 98-99 1.176913545015001 1105 100-101 1.4352027389346915 1106 1 0.12276835224041704 1106 2 -0.059006492280392564 1106 3 0.02460981238012394 1106 4 0.20691234316034723 1106 5 0.3051760202391449 1106 6 0.1610829162318339 1106 7 0.1922917310151675 1106 8 0.30018721544885096 1106 9 0.3154316884795918 1106 10-11 0.3164220072482067 1106 12-13 0.4414247239743787 1106 14-15 0.22175153470058717 1106 16-17 0.1746374153679895 1106 18-19 0.3932726501776358 1106 20-21 0.12340067958890444 1106 22-23 -0.07264059659224387 1106 24-25 -0.1775827751887249 1106 26-27 -0.23631721259170746 1106 28-29 0.0013427439524846818 1106 30-31 -0.012188356252970323 1106 32-33 0.21080288736688146 1106 34-35 0.1352549516197854 1106 36-37 0.008371276546611739 1106 38-39 0.21983946958110323 1106 40-41 0.23123985431352168 1106 42-43 0.32832819175256844 1106 44-45 -0.001951797173973091 1106 46-47 0.10348489796279381 1106 48-49 0.2008092256668661 1106 50-51 0.19433492729702806 1106 52-53 0.2895583596236051 1106 54-55 0.47092562134470484 1106 56-57 0.5822928324499088 1106 58-59 0.4824781444321218 1106 60-61 0.5864972796820567 1106 62-63 0.3280273578518873 1106 64-65 0.3426583239762593 1106 66-67 0.1583411014020939 1106 68-69 0.30393197133398075 1106 70-71 0.086933170782725 1106 72-73 0.23997215661405846 1106 74-75 0.3516674706891365 1106 76-77 0.34519846396365494 1106 78-79 0.2312809103592599 1106 80-81 0.4012468454935245 1106 82-83 0.3831681881423066 1106 84-85 0.7112790433699239 1106 86-87 0.7710039733424168 1106 88-89 0.7734190342224423 1106 90-91 0.8280026168086501 1106 92-93 0.9301966422765524 1106 94-95 0.9374341702691638 1106 96-97 0.8165528299460227 1106 98-99 0.929852975748716 1106 100-101 1.7514606013006855 1107 1 0.2023997452736701 1107 2 0.17166623815329274 1107 3 0.17138879834547893 1107 4 0.32587985643635875 1107 5 0.3121192573740572 1107 6 0.17471651403180744 1107 7 0.319383888683646 1107 8 0.324222333668601 1107 9 0.27600666635289883 1107 10-11 0.44298174141181335 1107 12-13 0.32717098614405415 1107 14-15 0.34991496007042144 1107 16-17 0.16558670968459666 1107 18-19 0.582328927045765 1107 20-21 0.14163050478804706 1107 22-23 0.2529956445570818 1107 24-25 0.2698169434862052 1107 26-27 0.08129513963360324 1107 28-29 0.2522505309672809 1107 30-31 0.00991363989065519 1107 32-33 -0.0040976610589638085 1107 34-35 0.026687477065486576 1107 36-37 0.030254475275903303 1107 38-39 0.21800512737601707 1107 40-41 0.026381761476251597 1107 42-43 -0.10295460316461913 1107 44-45 0.07208569176268753 1107 46-47 0.12499223928280401 1107 48-49 0.2829708650966296 1107 50-51 0.1657119779671561 1107 52-53 0.1588078894314222 1107 54-55 0.2173063268700588 1107 56-57 0.34769607163944727 1107 58-59 0.2614226222422076 1107 60-61 0.21485480749846886 1107 62-63 0.1658905714376786 1107 64-65 0.20279195338579115 1107 66-67 0.4004648883852795 1107 68-69 0.3260334735782635 1107 70-71 0.21332152187009612 1107 72-73 0.43655299015811977 1107 74-75 0.4420806866456424 1107 76-77 0.34193675262214285 1107 78-79 0.3990703748933342 1107 80-81 0.44047578957621525 1107 82-83 0.3901983515637255 1107 84-85 0.47254982665478806 1107 86-87 0.5574280629871247 1107 88-89 0.3001918365678691 1107 90-91 0.4732490330749286 1107 92-93 0.43841957264069364 1107 94-95 0.2887146537373617 1107 96-97 0.5470003422737477 1107 98-99 0.8765024613031507 1107 100-101 1.2051980365010877 1108 1 0.16922382264210967 1108 2 0.19170483611988942 1108 3 0.18889804352881967 1108 4 0.26678615394258287 1108 5 0.18915131093682191 1108 6 0.0820285645757366 1108 7 0.3656378374281388 1108 8 0.42203799064265013 1108 9 -0.0836433643745309 1108 10-11 0.04990619505652205 1108 12-13 0.08424206893561603 1108 14-15 0.5088501737069322 1108 16-17 0.6877061477499495 1108 18-19 0.48482755239750475 1108 20-21 0.44411619227389565 1108 22-23 0.44006586482424126 1108 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-3.023632099939867 2316 44-45 -3.234494056501944 2316 46-47 -3.2179774938121 2316 48-49 -3.952065986918168 2316 50-51 -3.6721028883595395 2316 52-53 -3.5408994390261554 2316 54-55 -2.9747814493623643 2316 56-57 -3.9674738071604736 2316 58-59 -3.7363523802019785 2316 60-61 -3.7992205491161286 2316 62-63 -3.3964503593713644 2316 64-65 -3.743235692721324 2316 66-67 -3.647083198999887 2316 68-69 -3.8353088102059054 2316 70-71 -3.633423295667491 2316 72-73 -2.6341919976840273 2316 74-75 -3.098539316571223 2316 76-77 -3.4214636933235028 2316 78-79 -3.65197447512827 2316 80-81 -4.395930121884337 2316 82-83 -3.4189682914495307 2316 84-85 -3.7286334746675927 2316 86-87 -4.289077066649337 2316 88-89 -5.684881284010913 2316 90-91 -4.709058846034061 2316 92-93 -4.288122550931032 2316 94-95 -3.5620496668262795 2316 96-97 -4.832794598419262 2316 98-99 -6.648163315995252 2316 100-101 -7.807441558047904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2864.0 3 1065.0 4 1109.0 5 1122.0 6 1197.0 7 1404.0 8 1823.0 9 2262.0 10 2921.0 11 3594.0 12 4605.0 13 5640.0 14 6720.0 15 8367.0 16 10542.0 17 13091.0 18 10614.0 19 7901.0 20 7704.0 21 8121.0 22 9098.0 23 10313.0 24 11760.0 25 13060.0 26 15247.0 27 18211.0 28 21453.0 29 25739.0 30 31197.0 31 38240.0 32 47340.0 33 61274.0 34 81541.0 35 111382.0 36 155766.0 37 202577.0 38 157101.0 39 24201.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.966013369569325 17.829023201996907 14.604975210113958 22.599988218319808 2 15.480950916201607 19.609614624071714 39.572790789647144 25.336643670079535 3 20.23523209068342 23.5073921926144 29.95672327057842 26.300652446123767 4 12.395284564991014 15.943811980157562 37.76320009842131 33.89770335643012 5 13.583252190847128 36.85500511461303 34.221330924251 15.340411770288846 6 31.70076002587322 35.462044406322065 17.280394004162215 15.5568015636425 7 28.161456190144584 32.83057422511087 20.436392795720483 18.57157678902406 8 26.539090746863277 32.313939085999905 19.870495995414792 21.27647417172203 9 25.921979455691886 16.96250540685959 21.067322874796474 36.04819226265205 10-11 25.247770635685303 25.42569781916128 26.971220453890137 22.35531109126328 12-13 26.913224555123076 23.250100966066174 27.35316853268949 22.483505946121255 14-15 23.225023579038783 24.47319636834458 26.940095617067623 25.361684435549016 16-17 21.84189902955465 27.92701639151156 27.463836307288208 22.76724827164558 18-19 21.64102090777636 26.53498767157606 29.975475006922377 21.848516413725207 20-21 22.733022465359078 25.097792480029597 29.553254337776963 22.615930716834367 22-23 21.945334265356973 24.99842929705881 30.17652064914187 22.87971578844235 24-25 22.522332119145645 25.168880472083206 29.90583820692746 22.40294920184369 26-27 21.905839064217094 25.104324713609603 30.802165542459946 22.187670679713353 28-29 21.295996091776477 26.280167259314318 30.29782631425878 22.126010334650424 30-31 21.581291387673733 25.3744823932432 30.0585325052211 22.985693713861977 32-33 21.376745534704206 25.54340304854876 30.244687885104995 22.83516353164204 34-35 22.67720562165468 25.311285354463962 29.67272915790727 22.338779865974086 36-37 22.26929703788898 24.928106447267957 30.454398883793836 22.34819763104923 38-39 21.874890166665494 25.552417739225792 30.146043188224457 22.426648905884257 40-41 22.003102431643924 25.756596621064464 30.142131083228374 22.098169864063237 42-43 21.618890808162632 25.922935399354397 29.24249799237718 23.215675800105785 44-45 21.861177188970967 25.542808568962222 29.37661305024919 23.21940119181762 46-47 22.729698848293395 26.110138066853196 28.94518927358183 22.214973811271587 48-49 22.55134100761395 26.03832981600353 28.61697772045399 22.79335145592853 50-51 21.70744339924765 26.497635018334265 29.395314142577185 22.399607439840903 52-53 22.55448582217669 26.647006728250037 28.125411839541165 22.673095610032103 54-55 21.75433201617491 26.023770353172708 28.82428784487549 23.397609785776886 56-57 22.490351688372165 26.171580697665735 28.116882403720062 23.221185210242037 58-59 23.070036014806576 25.8315519227373 28.133591297402877 22.964820765053247 60-61 22.387122708132438 25.987246595833945 29.415992496447014 22.2096381995866 62-63 21.973037040681664 26.305359380189326 29.124749374864912 22.596854204264098 64-65 21.60962167248516 26.850881736244826 28.700368702503688 22.839127888766324 66-67 21.858762482625803 26.440674689988068 28.023461977082754 23.67710085030338 68-69 21.88756978454434 26.12920486475373 28.786772523033072 23.19645282766886 70-71 22.500029653163423 26.159932909168624 28.121302964393802 23.218734473274154 72-73 22.166053941761035 25.87865278731613 29.01240345024098 22.94288982068185 74-75 21.7173017507724 27.217551308773547 28.201635835123 22.86351110533105 76-77 21.978929755305348 26.38490985211458 28.235542767956634 23.400617624623433 78-79 22.12029635149516 26.368991179931335 28.44882962952548 23.061882839048025 80-81 22.62589001939304 26.504103117912685 27.94091931984637 22.929087542847903 82-83 22.316929107049578 26.221838161934713 28.655344307753545 22.805888423262164 84-85 22.099190966376955 26.551160306041044 28.08149730966447 23.26815141791753 86-87 22.02406238454567 26.87401184273127 28.115877597873425 22.98604817484964 88-89 21.926016519457058 26.728010592414336 27.410156452490085 23.935816435638518 90-91 21.939553958383215 26.274368532385573 27.967595755289963 23.81848175394125 92-93 22.485457964290738 26.358911500070292 28.351917264164207 22.803713271474766 94-95 21.99064188743904 26.02715170686701 28.445894292869383 23.536312112824568 96-97 21.181888996137722 26.07781051869432 29.24060648850082 23.499693996667133 98-99 21.463829592779188 26.70434023795777 27.929544182302475 23.902285986960567 100-101 21.301212018691054 26.177802285194566 28.347045525944054 24.173940170170326 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 60.0 1 57.0 2 79.0 3 118.5 4 115.0 5 113.0 6 229.0 7 522.5 8 908.5 9 1110.5 10 1022.5 11 935.5 12 1009.5 13 1133.0 14 1232.5 15 1276.0 16 1353.0 17 1471.5 18 1626.0 19 1832.5 20 2002.5 21 2296.5 22 2687.0 23 3282.0 24 3926.5 25 4652.0 26 6086.0 27 7769.5 28 9244.5 29 10957.5 30 13269.0 31 15939.0 32 19056.0 33 21155.5 34 22524.5 35 24364.0 36 26485.0 37 28579.0 38 29718.5 39 30244.0 40 30799.5 41 31747.0 42 32489.0 43 33599.0 44 35175.5 45 37060.5 46 39012.0 47 45986.5 48 59965.5 49 58560.5 50 44501.5 51 38335.5 52 36299.5 53 36093.5 54 36166.0 55 33893.0 56 31072.0 57 28713.0 58 27100.0 59 24420.0 60 20351.5 61 17137.5 62 14523.0 63 11296.0 64 8174.5 65 6284.0 66 4623.5 67 3526.0 68 2686.5 69 1909.5 70 1444.0 71 1064.5 72 851.0 73 748.5 74 612.0 75 502.0 76 384.0 77 227.0 78 133.5 79 85.5 80 58.0 81 49.5 82 34.0 83 21.5 84 12.0 85 7.0 86 8.5 87 7.0 88 6.0 89 4.0 90 1.5 91 3.0 92 2.5 93 1.5 94 0.5 95 1.5 96 2.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07283506766896154 2 0.029696324333062726 3 0.014321008480145636 4 0.019592545344002923 5 0.02389763378281971 6 0.029169170646676998 7 0.07591013083954495 8 0.05420897074999913 9 0.06536705711183038 10-11 0.056581162338734906 12-13 0.03773541805044509 14-15 0.046125947558751275 16-17 0.05425290022386459 18-19 0.05034317704983711 20-21 0.01682498849047785 22-23 0.014277079006280157 24-25 0.02222831377593157 26-27 0.024117281152147098 28-29 0.006589421079821611 30-31 0.0014936021114262319 32-33 0.009269118985615733 34-35 0.010938438992503874 36-37 0.004129370543354877 38-39 0.008785894773095482 40-41 0.004261158964951308 42-43 0.002284332641004825 44-45 9.225189511750256E-4 46-47 6.589421079821611E-4 48-49 0.0012300252682333674 50-51 0.001625390533022664 52-53 1.7571789546190963E-4 54-55 4.832242125202515E-4 56-57 0.003118992644448896 58-59 0.003953652647892967 60-61 0.005315466337722766 62-63 0.0029872042228524637 64-65 6.589421079821611E-4 66-67 5.271536863857289E-4 68-69 0.0036900758047001023 70-71 0.0025918389580631674 72-73 0.020954359033832724 74-75 0.015463174800648048 76-77 0.024600505364667347 78-79 0.018889673762155286 80-81 0.013266701107374178 82-83 0.004964030546798947 84-85 0.003514357909238193 86-87 0.0018889673762155284 88-89 0.0013178842159643222 90-91 0.00575476107637754 92-93 0.00948876635494312 94-95 0.012124534786871765 96-97 0.011289874783427694 98-99 0.022404031671393477 100-101 0.01849430849736599 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1138188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.391471160083164 #Duplication Level Percentage of deduplicated Percentage of total 1 82.4764238224685 49.80872570661403 2 10.467410073413394 12.64284587138618 3 3.0369394171607715 5.502157176791535 4 1.2780155844808676 3.0872496524925257 5 0.6782400193125493 2.0479956282964036 6 0.4260494639840023 1.5437852350175256 7 0.28605406132059225 1.2092657916127048 8 0.2056830593882156 0.9937202039328876 9 0.1640983087231896 0.8919124450807434 >10 0.8741898561511555 9.850271109050253 >50 0.06732268499072669 2.776838157823776 >100 0.033271182754992475 3.7923451482397925 >500 0.003517655358736399 1.4262447606477546 >1k 0.002491672545771616 3.323921259346148 >5k 2.931379465613666E-4 1.102721853667829 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6235 0.5478005391025033 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6223 0.5467462317297318 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4841 0.42532516596555225 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4450 0.3909723174027489 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3739 0.32850460556604005 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3698 0.3249023887090709 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2809 0.24679578417625206 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2244 0.1971554787082626 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2140 0.1880181481442433 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2025 0.1779143691551835 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1703 0.14962378798581605 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1573 0.13820212478079194 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1339 0.1176431310117485 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1279 0.11237159414789122 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1251 0.10991154361142447 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1202 0.1056064551726077 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.392947386547741E-5 0.0 18-19 0.0 0.0 0.0 8.785894773095482E-5 1.3178842159643222E-4 20-21 0.0 0.0 0.0 8.785894773095482E-5 1.7571789546190963E-4 22-23 0.0 0.0 0.0 2.6357684319286445E-4 1.7571789546190963E-4 24-25 0.0 0.0 0.0 3.9536526478929667E-4 1.7571789546190963E-4 26-27 0.0 0.0 0.0 6.589421079821611E-4 1.7571789546190963E-4 28-29 0.0 0.0 0.0 0.0014936021114262319 1.7571789546190963E-4 30-31 0.0 0.0 0.0 0.0031629221183143734 1.7571789546190963E-4 32-33 0.0 0.0 0.0 0.0047883126513370376 1.7571789546190963E-4 34-35 0.0 0.0 0.0 0.006765138975283521 2.6357684319286445E-4 36-37 0.0 0.0 0.0 0.008610176877633572 2.6357684319286445E-4 38-39 0.0 0.0 0.0 0.011729169522082469 2.6357684319286445E-4 40-41 0.0 0.0 0.0 0.014321008480145635 2.6357684319286445E-4 42-43 0.0 0.0 0.0 0.021745089563411316 2.6357684319286445E-4 44-45 8.785894773095482E-5 0.0 0.0 0.0318927980263366 2.6357684319286445E-4 46-47 8.785894773095482E-5 0.0 0.0 0.04283123701884047 2.6357684319286445E-4 48-49 8.785894773095482E-5 0.0 0.0 0.05605400865234917 2.6357684319286445E-4 50-51 8.785894773095482E-5 0.0 0.0 0.07252756135190319 2.6357684319286445E-4 52-53 8.785894773095482E-5 0.0 0.0 0.0993684698837099 2.6357684319286445E-4 54-55 8.785894773095482E-5 0.0 0.0 0.12111355944712121 2.6357684319286445E-4 56-57 8.785894773095482E-5 0.0 0.0 0.16723950700587248 2.6357684319286445E-4 58-59 8.785894773095482E-5 0.0 0.0 0.22478711776964788 2.6357684319286445E-4 60-61 8.785894773095482E-5 0.0 0.0 0.2813682801083828 2.6357684319286445E-4 62-63 8.785894773095482E-5 0.0 0.0 0.35965060253666353 2.6357684319286445E-4 64-65 8.785894773095482E-5 0.0 0.0 0.41008163853423163 2.6357684319286445E-4 66-67 8.785894773095482E-5 0.0 0.0 0.5105044157907129 2.6357684319286445E-4 68-69 8.785894773095482E-5 0.0 0.0 0.6295972194400222 2.6357684319286445E-4 70-71 8.785894773095482E-5 0.0 0.0 0.7111742524082139 2.6357684319286445E-4 72-73 8.785894773095482E-5 0.0 0.0 0.8558340098472308 3.0750631705834185E-4 74-75 8.785894773095482E-5 0.0 0.0 0.9955297367394489 3.5143579092381926E-4 76-77 8.785894773095482E-5 0.0 0.0 1.0876059139614895 3.5143579092381926E-4 78-79 8.785894773095482E-5 0.0 0.0 1.1944423944023308 3.5143579092381926E-4 80-81 1.7571789546190963E-4 0.0 0.0 1.3890499636263955 3.5143579092381926E-4 82-83 1.7571789546190963E-4 0.0 0.0 1.5540929969389943 3.5143579092381926E-4 84-85 1.7571789546190963E-4 0.0 0.0 1.801855229540287 3.5143579092381926E-4 86-87 1.7571789546190963E-4 0.0 0.0 2.1557950004744386 3.5143579092381926E-4 88-89 1.7571789546190963E-4 0.0 0.0 2.6069506970728913 3.5143579092381926E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAC 70 0.00836948 27.157505 3 GTACATG 4995 0.0 25.690662 1 TACATGG 5015 0.0 25.30278 2 ACATGGG 4965 0.0 24.504658 3 ATACCGT 125 2.2469876E-4 22.813307 6 GAGTACT 4655 0.0 22.669273 12-13 AGTACTT 4895 0.0 22.14045 12-13 TACCTGG 870 0.0 21.850866 2 ACGTATA 110 0.0028683546 21.60256 2 GTACTTT 4945 0.0 20.99693 14-15 TATTCCG 140 4.8326422E-4 20.369024 5 CCTATAC 280 1.5643309E-9 20.36813 3 GTACCTG 940 0.0 20.224564 1 GTGTAGC 525 0.0 19.91638 1 ATAGGAC 215 1.0732056E-6 19.894453 3 GTATAGG 335 3.45608E-11 19.862333 1 AGAGTAC 8070 0.0 19.585808 10-11 CATGGGA 2525 0.0 19.575775 4 TAAGGTG 540 0.0 19.363146 5 TACACCG 175 9.892259E-5 19.01109 4 >>END_MODULE