FastQCFastQC Report
Thu 26 May 2016
SRR936684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138188
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121371.0663440486105986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44330.3894787152913227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39160.344055639314419No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34160.30012616544894166No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33150.2912524117281152No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30810.2706934179590718No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29810.2619075231859763No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22560.19820978608103404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.19820978608103404No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21000.1845037902350051No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19540.1716763838662857No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA17920.15744323433387103No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA17330.1522595564177447No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16020.1407500342649896No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15620.13723567635575143No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC14480.1272197563144226No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC14080.12370539840518438No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA11980.10525501938168386No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11790.10358569937479574No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA11700.10279496884521713No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA11590.10182852042017662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT4900.031.0150554
TAAGGTG6250.028.875025
GTACATG53500.024.776551
TACATGG55450.023.8153722
ACATGGG53550.023.5927733
GAGTACT48050.022.7822312-13
ATGGGTA4500.022.1628425
GTACTTT49950.021.91563814-15
GGTAAGG6750.021.8129673
AGGTAAG6200.021.4526252
GTCCTAC2457.499693E-921.3312871
GGGCGTA2457.537892E-921.3219159
AGTACTT50250.020.69792212-13
CATGGGG24850.020.4491884
GTGTAGC5050.019.7568821
GGTGTGC10950.019.0826268
GGACCGA1250.006038652718.9958886
TACTTTT55400.018.90242614-15
GGGGCGC4051.8189894E-1218.761376
TAGACTG3557.8216544E-1118.7291645
Produced by FastQC (version 0.11.5)