##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936684_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1138188 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.951356893588756 31.0 31.0 34.0 30.0 34.0 2 32.111715287808344 33.0 31.0 34.0 30.0 34.0 3 32.19390118328431 33.0 31.0 34.0 30.0 34.0 4 35.868783540153295 37.0 35.0 37.0 35.0 37.0 5 35.716144433081354 37.0 35.0 37.0 33.0 37.0 6 35.81400172906409 37.0 35.0 37.0 35.0 37.0 7 35.76176694886961 37.0 35.0 37.0 33.0 37.0 8 35.73478898037934 37.0 35.0 37.0 33.0 37.0 9 37.370549505002685 39.0 37.0 39.0 34.0 39.0 10-11 37.25301971203351 39.0 37.0 39.0 33.5 39.0 12-13 37.163950507297564 39.0 37.0 39.0 33.0 39.0 14-15 38.277455921165924 40.0 38.0 41.0 33.0 41.0 16-17 38.20398958695752 40.0 38.0 41.0 33.0 41.0 18-19 38.35951705693611 40.0 38.0 41.0 33.5 41.0 20-21 38.377432814262676 40.0 38.0 41.0 34.0 41.0 22-23 38.2960117309267 40.0 38.0 41.0 34.0 41.0 24-25 38.14736229867122 40.0 37.5 41.0 33.0 41.0 26-27 37.94050851001768 40.0 37.0 41.0 33.0 41.0 28-29 37.87895848489002 40.0 37.0 41.0 33.0 41.0 30-31 37.87653972805899 40.0 37.0 41.0 33.0 41.0 32-33 37.72800231596186 40.0 37.0 41.0 33.0 41.0 34-35 37.54962536944687 40.0 36.0 41.0 33.0 41.0 36-37 37.33832547874341 39.5 36.0 41.0 32.0 41.0 38-39 37.156158736518044 39.0 36.0 41.0 31.0 41.0 40-41 36.99414551901795 39.0 36.0 41.0 31.0 41.0 42-43 36.710638752121795 39.0 35.0 41.0 31.0 41.0 44-45 36.426163779621646 39.0 35.0 40.0 30.0 41.0 46-47 36.03707208299508 39.0 35.0 40.0 29.5 41.0 48-49 35.631074567646124 38.5 35.0 40.0 28.0 41.0 50-51 35.3130449451233 38.0 34.5 40.0 27.0 41.0 52-53 34.97062655729985 38.0 34.0 40.0 26.0 41.0 54-55 34.77666343345739 38.0 34.0 40.0 26.0 41.0 56-57 34.445362277585076 38.0 33.5 40.0 25.5 41.0 58-59 34.06680926173883 37.0 33.0 40.0 24.5 41.0 60-61 33.60284724491912 37.0 33.0 39.5 23.0 41.0 62-63 33.30644190590658 36.0 32.0 39.0 23.0 40.0 64-65 32.87659068624867 36.0 32.0 39.0 22.0 40.0 66-67 32.44162080429595 35.0 31.0 38.5 21.5 40.0 68-69 32.076240480483015 35.0 31.0 38.0 20.0 40.0 70-71 31.675555795703346 35.0 31.0 37.5 19.5 39.5 72-73 31.21085532442795 34.5 30.5 37.0 18.0 39.0 74-75 30.72363265119646 34.0 30.0 36.0 16.0 39.0 76-77 29.250861456982502 32.5 28.0 35.0 13.0 37.0 78-79 30.03105945590711 34.0 29.5 35.0 12.0 37.0 80-81 29.79298718665106 34.0 29.0 35.0 7.0 37.0 82-83 29.49256801161144 34.0 29.0 35.0 7.0 36.5 84-85 29.12990472575708 34.0 29.0 35.0 3.5 36.0 86-87 28.78566721842086 34.0 29.0 35.0 2.0 36.0 88-89 28.307790980048992 33.0 28.0 35.0 2.0 35.0 90-91 27.828996176378595 33.0 27.0 35.0 2.0 35.0 92-93 27.21401561077783 33.0 26.0 34.5 2.0 35.0 94-95 26.687639476079525 32.0 25.0 34.0 2.0 35.0 96-97 25.965637486952946 32.0 24.5 34.0 2.0 35.0 98-99 25.12716176940892 32.0 21.0 34.0 2.0 35.0 100-101 21.91932791419344 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2706169146924893 1101 2 0.25603264766811407 1101 3 0.3094976300729684 1101 4 0.26117814229534986 1101 5 0.23613385218988725 1101 6 0.14150311302216068 1101 7 0.23214002996992633 1101 8 0.19696244833127707 1101 9 0.0532146587139124 1101 10-11 0.32759436683534915 1101 12-13 0.2872910177274903 1101 14-15 0.48839753244367046 1101 16-17 0.5544751050357775 1101 18-19 0.5179986973673678 1101 20-21 0.3551395520906837 1101 22-23 0.2946517437438274 1101 24-25 0.37189048107111944 1101 26-27 0.27167020471637926 1101 28-29 0.3877830547804493 1101 30-31 0.4815895604449949 1101 32-33 0.4774288678288485 1101 34-35 0.46228790162820843 1101 36-37 0.48629963055679326 1101 38-39 0.4039942780713375 1101 40-41 0.44599572966212975 1101 42-43 0.48539424699993106 1101 44-45 0.4914128706954486 1101 46-47 0.4433085217696089 1101 48-49 0.2959388537090959 1101 50-51 0.5239666567767927 1101 52-53 0.6185341382928655 1101 54-55 0.3844435821022145 1101 56-57 0.4116537546883663 1101 58-59 0.4388014029720395 1101 60-61 0.4260589618760946 1101 62-63 0.6473978714557447 1101 64-65 0.5647693153869398 1101 66-67 0.5041785741113785 1101 68-69 0.34848392556906305 1101 70-71 0.48011407464135303 1101 72-73 0.5965330199638714 1101 74-75 0.6673532733943262 1101 76-77 0.547456101584995 1101 78-79 0.5953779538059187 1101 80-81 0.7408309476460317 1101 82-83 0.7332607242884492 1101 84-85 0.7039246718205554 1101 86-87 0.6461517017504796 1101 88-89 0.6380343689982801 1101 90-91 0.6752911459976758 1101 92-93 0.7499687175781311 1101 94-95 0.604550240734703 1101 96-97 0.6520030836059938 1101 98-99 0.49213483156979265 1101 100-101 1.1104867586305112 1102 1 0.25005594751080906 1102 2 0.24476903735939715 1102 3 0.296685137836576 1102 4 0.20495675471576646 1102 5 0.3090239014478513 1102 6 0.22622636725515832 1102 7 0.1146138143867077 1102 8 0.1117219967208527 1102 9 0.264240674419554 1102 10-11 0.21546574955915077 1102 12-13 0.38054464093225704 1102 14-15 0.3670880970922141 1102 16-17 0.3085782418717571 1102 18-19 0.21350472644335383 1102 20-21 0.3018862902465287 1102 22-23 0.3187461401699707 1102 24-25 0.23225509392968746 1102 26-27 0.23932413869128055 1102 28-29 0.2449597894807738 1102 30-31 0.3423374414037781 1102 32-33 0.34726903351269556 1102 34-35 0.30094264640597856 1102 36-37 0.38944753319721315 1102 38-39 0.31634287362073366 1102 40-41 0.311609331275605 1102 42-43 0.4739093033157218 1102 44-45 0.4320532920419211 1102 46-47 0.3645488469038227 1102 48-49 0.38595875112785194 1102 50-51 0.109132577760775 1102 52-53 0.25373901846793956 1102 54-55 0.13794256916325054 1102 56-57 0.0728411404927698 1102 58-59 0.2065219628099655 1102 60-61 0.20586048455938055 1102 62-63 0.1197799832019939 1102 64-65 0.02555626846200454 1102 66-67 -0.030037758936636294 1102 68-69 0.12735471882336213 1102 70-71 0.07801344022762535 1102 72-73 0.15404220642820476 1102 74-75 0.14879040455242176 1102 76-77 -0.018727320545178117 1102 78-79 0.19050478008858107 1102 80-81 0.251372062579037 1102 82-83 -0.015748583005841255 1102 84-85 0.22531541330994642 1102 86-87 0.11002083500446247 1102 88-89 -0.05446401052522276 1102 90-91 -0.0684508786606699 1102 92-93 0.3318227685435282 1102 94-95 0.1674554445533829 1102 96-97 0.3981935522681219 1102 98-99 0.48740078168847845 1102 100-101 1.038176015605334 1103 1 0.23427458547025992 1103 2 0.22735426965873984 1103 3 0.2001679982520983 1103 4 0.22033255907958704 1103 5 0.21512170091332194 1103 6 0.21880480145796355 1103 7 0.1738070758899184 1103 8 0.17950693474642065 1103 9 0.33455767059213315 1103 10-11 0.2711307523994506 1103 12-13 0.26863516736568016 1103 14-15 0.3463834697303412 1103 16-17 0.2823126784213059 1103 18-19 0.2966554579371632 1103 20-21 0.16118105757468726 1103 22-23 0.098125324957131 1103 24-25 0.26627677333890887 1103 26-27 0.27737834652678117 1103 28-29 0.17166327140994753 1103 30-31 0.12869282354061795 1103 32-33 0.11121351262018209 1103 34-35 0.15821358314642708 1103 36-37 0.1419679507679632 1103 38-39 0.22197202348617395 1103 40-41 0.23341096654375093 1103 42-43 0.21810484637188665 1103 44-45 0.2584997500473776 1103 46-47 0.27255919304798937 1103 48-49 0.15125446832703204 1103 50-51 0.1832849109978838 1103 52-53 0.35586062989619904 1103 54-55 0.2544146829987639 1103 56-57 0.08291279988019795 1103 58-59 0.24309758464955422 1103 60-61 0.18984747505135857 1103 62-63 0.4293848554815938 1103 64-65 0.16691057966582434 1103 66-67 0.10287015013650347 1103 68-69 0.3935756290232959 1103 70-71 0.41936565612772014 1103 72-73 0.3132296476432934 1103 74-75 0.22065163957099898 1103 76-77 0.22230477950790117 1103 78-79 0.123841295179421 1103 80-81 0.2721216225652512 1103 82-83 0.22051553701315996 1103 84-85 0.7423001781237453 1103 86-87 0.6654469732200106 1103 88-89 0.3440792877622165 1103 90-91 0.5035747212946298 1103 92-93 0.5755796946864002 1103 94-95 0.43750773402192067 1103 96-97 0.400181529880971 1103 98-99 0.44900690183784775 1103 100-101 0.7832283050738731 1104 1 0.10852276796740057 1104 2 0.11926791818346771 1104 3 0.171198562552469 1104 4 0.2745891555041382 1104 5 0.18604638208219626 1104 6 0.05116013390264129 1104 7 0.1869701453662742 1104 8 0.10960468756603348 1104 9 0.02913068741052882 1104 10-11 0.2379238643235979 1104 12-13 0.15054073284778013 1104 14-15 0.2925832579640826 1104 16-17 0.3222459815023129 1104 18-19 0.13262145243502488 1104 20-21 0.061127643316389424 1104 22-23 -0.0033468759939196957 1104 24-25 0.05202272945413 1104 26-27 0.13381571003680648 1104 28-29 0.2863498289791693 1104 30-31 0.25203910864985346 1104 32-33 0.262188204660994 1104 34-35 0.20103314694574692 1104 36-37 0.08810841608651288 1104 38-39 0.2245120369106388 1104 40-41 0.2119963395454434 1104 42-43 0.22963807193131913 1104 44-45 0.1989433247375274 1104 46-47 0.2978754243648254 1104 48-49 0.5865546753663651 1104 50-51 0.5742980802123583 1104 52-53 0.46160313535580144 1104 54-55 0.5369063872444926 1104 56-57 0.6010738840478282 1104 58-59 0.6639414353921538 1104 60-61 0.7784912728523921 1104 62-63 0.48696164438655387 1104 64-65 0.785911402150866 1104 66-67 0.7819643766696842 1104 68-69 0.7214644895506339 1104 70-71 0.8804623501160158 1104 72-73 1.1339092377243105 1104 74-75 0.8905673584891005 1104 76-77 0.701641024627353 1104 78-79 0.8812174260963488 1104 80-81 1.0212745575133049 1104 82-83 0.8033619970186372 1104 84-85 0.966582158138305 1104 86-87 0.925648387516123 1104 88-89 1.007817860419788 1104 90-91 1.02527910882689 1104 92-93 1.2395316880308904 1104 94-95 0.9086926324402924 1104 96-97 1.1078587659530825 1104 98-99 1.220244032783139 1104 100-101 1.857052955045038 1105 1 -0.028258680630528943 1105 2 0.11886569452170903 1105 3 0.11594149929327102 1105 4 0.12463170653361999 1105 5 0.19516204043765129 1105 6 0.06130463804554154 1105 7 0.022879827101505157 1105 8 0.13575769344696909 1105 9 -0.021087995243469493 1105 10-11 0.16654610009440063 1105 12-13 0.1624402023359366 1105 14-15 0.1959649004761559 1105 16-17 0.21646750935947523 1105 18-19 0.11339939533619514 1105 20-21 0.17684950777125152 1105 22-23 0.2991994114816805 1105 24-25 0.37188368708515185 1105 26-27 0.058729020252862085 1105 28-29 0.11500678300289024 1105 30-31 0.12437966930730227 1105 32-33 7.322396840905299E-4 1105 34-35 0.13244554474918147 1105 36-37 0.07062862253309987 1105 38-39 0.0605254500112693 1105 40-41 0.062140504973690724 1105 42-43 0.05540536649029093 1105 44-45 0.11358934674551335 1105 46-47 5.580444920951777E-4 1105 48-49 0.06723947173534839 1105 50-51 0.13881898949186677 1105 52-53 0.3162903111952744 1105 54-55 0.17234779595877825 1105 56-57 -0.02266493307588746 1105 58-59 0.019659573985727263 1105 60-61 0.08176579685245144 1105 62-63 0.01682357582223659 1105 64-65 0.09297953037130213 1105 66-67 0.04697049470326675 1105 68-69 0.09028030075108262 1105 70-71 -0.13987111446924771 1105 72-73 0.22032804993666844 1105 74-75 0.2535911489602114 1105 76-77 0.0386642308156695 1105 78-79 0.1322960205998598 1105 80-81 0.2854042513575905 1105 82-83 0.18746654129923357 1105 84-85 0.2738492812229758 1105 86-87 0.3846808490825957 1105 88-89 0.3297767319918883 1105 90-91 0.3427859995533069 1105 92-93 0.32578325987861945 1105 94-95 0.4679825171502898 1105 96-97 0.45459249812293123 1105 98-99 0.3631075265402046 1105 100-101 1.0159355353733375 1106 1 0.03359354561970207 1106 2 0.10518934126521629 1106 3 -0.04413656276906863 1106 4 0.16274772097249013 1106 5 0.14811710875889617 1106 6 -0.09767830875374273 1106 7 -0.14180430331306582 1106 8 -0.08831295940052541 1106 9 -0.09715154484996447 1106 10-11 0.004758554391983694 1106 12-13 0.018784996132801268 1106 14-15 0.11728750148625977 1106 16-17 -0.04191288197720411 1106 18-19 -0.07819179455349712 1106 20-21 -0.09335953240040595 1106 22-23 -0.08031781794448278 1106 24-25 -0.0741876958391785 1106 26-27 -0.11619458311206898 1106 28-29 -0.38525803929989166 1106 30-31 -0.21170646039462326 1106 32-33 -0.04047546620920883 1106 34-35 0.0663597926732038 1106 36-37 -0.22419993528433935 1106 38-39 -0.12930735851881536 1106 40-41 0.11573603659356735 1106 42-43 0.2137792704067607 1106 44-45 -0.009576020500567495 1106 46-47 0.03644267685870517 1106 48-49 0.026254468327032043 1106 50-51 0.021001870835988257 1106 52-53 -0.14255838473643223 1106 54-55 -0.2202311762789435 1106 56-57 -0.25684046911108993 1106 58-59 -0.1413134131711189 1106 60-61 -0.19754063983033632 1106 62-63 -0.12486386027183016 1106 64-65 -0.2054761731361694 1106 66-67 -0.1964653495521702 1106 68-69 -0.34875586225938804 1106 70-71 -0.32319544287601687 1106 72-73 -0.11119146848373163 1106 74-75 -0.017706470640707295 1106 76-77 -0.14520378213593332 1106 78-79 -0.294884381968771 1106 80-81 -0.11168033104620534 1106 82-83 -0.14042099007277287 1106 84-85 0.012877310751388649 1106 86-87 -0.06649671451348738 1106 88-89 0.014945375869313438 1106 90-91 0.23187289999948746 1106 92-93 0.11854552594418166 1106 94-95 0.1710975923656335 1106 96-97 0.5633119781997742 1106 98-99 0.6372930164082078 1106 100-101 0.902968731599401 1107 1 -0.0010722623206191884 1107 2 0.07976439026003135 1107 3 -0.008422700397090921 1107 4 -0.02915588567816485 1107 5 0.013967129305108017 1107 6 -0.020996482669531247 1107 7 -0.08478362275926798 1107 8 0.00316707916313419 1107 9 0.029183959006402915 1107 10-11 0.029313254733359884 1107 12-13 -0.12026446492422593 1107 14-15 -0.031859237987760025 1107 16-17 -0.0029955883876198186 1107 18-19 -0.1006815016909357 1107 20-21 -0.05645092748827807 1107 22-23 -0.10602379768275938 1107 24-25 0.02051306041951051 1107 26-27 -0.05192938681671677 1107 28-29 -0.16501152425374244 1107 30-31 -0.21069693011267532 1107 32-33 -0.07583470884417665 1107 34-35 -0.12266882583102756 1107 36-37 -0.2534318625191361 1107 38-39 -0.1508645836737017 1107 40-41 0.07203433995050545 1107 42-43 0.07862173626843116 1107 44-45 0.03937977748274335 1107 46-47 -0.17016233799778746 1107 48-49 -0.0730153508662923 1107 50-51 -0.2237692166908829 1107 52-53 0.00707188220629007 1107 54-55 -0.25416601317361653 1107 56-57 -0.24488217792383438 1107 58-59 -0.33819206802635193 1107 60-61 -0.365166623677851 1107 62-63 -0.20284217216334355 1107 64-65 -0.2539069561049345 1107 66-67 -0.18293300111999855 1107 68-69 -0.28290426123523815 1107 70-71 -0.1392169452783385 1107 72-73 -0.06866920350079653 1107 74-75 0.0046681579469805 1107 76-77 -0.1020231493125685 1107 78-79 -0.06018093889758802 1107 80-81 -0.11345194803318037 1107 82-83 -0.23283005332350015 1107 84-85 0.04478222129634091 1107 86-87 0.06152743123818638 1107 88-89 0.11425618870911691 1107 90-91 -0.016662194513092032 1107 92-93 -0.1353849251242174 1107 94-95 0.05259385058411681 1107 96-97 0.26968683595675813 1107 98-99 0.07490760105860872 1107 100-101 0.5957761740700036 1108 1 -0.06681387157550489 1108 2 0.061680488199677086 1108 3 -0.12682573648295659 1108 4 0.030834842184610523 1108 5 0.03801646570040873 1108 6 -0.02903952263675791 1108 7 -0.07838045729474175 1108 8 -0.009571327969943866 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-0.719782179629668 2316 66-67 -0.7385177292161558 2316 68-69 -1.2194800041888882 2316 70-71 -0.9961027686851267 2316 72-73 -0.483388405970846 2316 74-75 -0.7049665072310987 2316 76-77 -0.5186093943808316 2316 78-79 -0.8729973032736567 2316 80-81 -0.46009190211402284 2316 82-83 -0.5786255806211109 2316 84-85 -0.684457968057103 2316 86-87 -0.31683411273035134 2316 88-89 -0.6975454443223974 2316 90-91 -0.6579999999180579 2316 92-93 -0.7876545487826796 2316 94-95 -0.5318575006564252 2316 96-97 -0.7399180259230889 2316 98-99 -1.5405762373538998 2316 100-101 -2.9151051835727273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 20.0 5 36.0 6 63.0 7 176.0 8 428.0 9 731.0 10 1253.0 11 1999.0 12 2835.0 13 3906.0 14 5270.0 15 7269.0 16 9482.0 17 12190.0 18 11122.0 19 7690.0 20 6984.0 21 7183.0 22 7626.0 23 8438.0 24 9910.0 25 12027.0 26 14356.0 27 17964.0 28 22344.0 29 27415.0 30 34028.0 31 41907.0 32 52639.0 33 67626.0 34 89868.0 35 123415.0 36 175414.0 37 219352.0 38 128031.0 39 7184.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.275178100200094 19.193696215145568 14.625086666496776 21.906039018157564 2 16.199217085290254 21.617760125828717 37.557938763943746 24.625084024937284 3 21.189081850971572 24.23730675113456 29.142478066610728 25.43113333128314 4 13.240431282002621 16.86637005485913 36.856916432083274 33.036282231054976 5 13.95744830403859 38.375163094333324 32.84968084031472 14.817707761313365 6 32.21102313501812 36.681989267150946 16.031270756676403 15.075716841154538 7 29.332324712613385 33.06105845431511 19.466819189799928 18.139797643271585 8 27.306473095833027 33.25733534354606 18.59648845357709 20.839703107043828 9 26.502915159885713 17.7342407405455 19.912703349534524 35.850140750034264 10-11 25.824600153928877 26.29183403796209 26.086463747640988 21.797102060468042 12-13 27.63284828910768 23.810335465981595 26.365427903192646 22.19138834171808 14-15 23.29817942681575 25.81086299201668 25.984955879336013 24.906001701831556 16-17 22.276118829279007 28.76068310361869 26.41533139311405 22.54786667398825 18-19 22.121641704823663 27.687371971071116 28.852794901994034 21.338191422111187 20-21 23.37513662066372 25.749305035723445 28.815143016795115 22.060415326817715 22-23 22.530548556126053 25.953577089197914 28.908405289811522 22.607469064864503 24-25 23.375224479611454 26.35992472245358 28.760231174463268 21.504619623471694 26-27 22.679370724757757 25.979898371635436 29.598701068780358 21.74202983482645 28-29 21.83941575055529 27.747631231197456 28.823988880141705 21.58896413810555 30-31 22.345398074971556 26.221988903190663 28.81836113393049 22.614251887907287 32-33 22.185093396375212 26.65947326175368 28.97038387915783 22.185049462713284 34-35 23.279814297086006 26.16881428127103 28.864124790671276 21.68724663097169 36-37 23.201335819177668 25.8318118958546 29.202550040503333 21.7643022444644 38-39 22.460796786425956 26.711208850683377 29.0580421939742 21.769952168916472 40-41 22.66774206443864 26.584641228764955 28.756435144245195 21.991181562551205 42-43 22.239111658602205 27.15519703089566 27.89307990978112 22.712611400721013 44-45 22.77371345680493 26.427165332711272 28.108041380952443 22.69107982953135 46-47 23.633207094280408 27.382950655188154 27.365203093171626 21.618639157359812 48-49 23.53474535245273 27.34714425912926 27.124552246083883 21.993558142334127 50-51 22.49949041810316 27.638579918256035 27.94511978688934 21.91680987675147 52-53 23.501309760140153 27.87304881322069 26.73000139256738 21.895640034071775 54-55 22.45895453049817 27.506010806052057 27.31504443361799 22.719990229831787 56-57 23.25159900191173 27.59141123864266 26.928259879057148 22.22872988038846 58-59 23.940539526283107 26.86113892962183 26.593217009724203 22.605104534370863 60-61 22.774594768694424 27.569921596766672 27.9841058019623 21.671377832576603 62-63 22.432968582833368 27.503696201864248 27.947372236636003 22.115962978666378 64-65 22.26391566130066 28.3363600133751 27.216393196275185 22.183331129049055 66-67 22.73334519471334 27.475832438238452 26.88219233447334 22.908630032574866 68-69 22.612529778992975 27.519984817820436 27.10405635681285 22.76342904637374 70-71 23.249089670278114 27.70490600617737 26.673264882336944 22.37273944120757 72-73 23.0170775904673 27.07726099610126 27.59040140574378 22.315260007687662 74-75 22.62216770971622 28.844530118110757 26.49246289435049 22.04083927782254 76-77 23.15090560761089 27.370616658400387 26.668166164905855 22.810311569082863 78-79 23.0563920246105 27.928625342800984 26.76914303489035 22.24583959769817 80-81 23.484173433161097 27.94588561988119 26.55466800239024 22.01527294456747 82-83 23.143322146382957 27.78397322162201 27.014595762667927 22.058108869327103 84-85 22.540288025875746 28.447894739155082 26.67245296617432 22.339364268794853 86-87 22.74301787413676 28.307848855856655 26.300358607502726 22.648774662503858 88-89 22.377615615834713 28.544555295313128 25.824389544964482 23.25343954388767 90-91 22.897077543040396 27.698200320693147 26.328133790930575 23.07658834533588 92-93 23.26746466110432 28.285091261934543 26.437044777982173 22.010399298978967 94-95 22.745885969977667 27.907595057978313 26.51876110192634 22.82775787011768 96-97 22.07674495068933 28.008179470092713 27.349619598376062 22.565455980841893 98-99 22.293430699288834 28.58108696206438 25.93568642207368 23.189795916573107 100-101 21.86918739221054 28.476596141274698 26.196416301305366 23.4578001652094 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 120.0 1 95.0 2 90.5 3 112.0 4 106.0 5 118.5 6 217.0 7 427.5 8 695.5 9 877.5 10 900.0 11 899.0 12 969.0 13 1095.5 14 1221.5 15 1329.5 16 1468.0 17 1598.5 18 1722.5 19 1865.0 20 2048.5 21 2346.0 22 2720.5 23 3243.0 24 3838.5 25 4581.5 26 5858.0 27 7546.5 28 9246.5 29 11171.0 30 13355.0 31 15669.0 32 18635.5 33 20694.5 34 22082.5 35 23840.0 36 26276.5 37 28844.0 38 29942.0 39 30131.5 40 30262.0 41 31449.0 42 32353.0 43 33683.0 44 35556.0 45 36882.0 46 37647.5 47 43926.5 48 60494.5 49 59839.0 50 44561.5 51 38260.5 52 35789.0 53 36053.5 54 36647.5 55 34423.0 56 31113.5 57 28777.5 58 27906.5 59 25608.0 60 21151.5 61 17413.0 62 14736.5 63 11623.0 64 8283.0 65 6256.0 66 4754.0 67 3696.5 68 2748.0 69 1819.0 70 1365.0 71 1116.5 72 896.0 73 763.5 74 641.0 75 523.5 76 388.5 77 233.5 78 159.5 79 125.0 80 93.0 81 79.5 82 56.5 83 33.5 84 22.0 85 16.5 86 11.5 87 5.5 88 2.0 89 2.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018011084284845737 2 0.01168524004821699 3 0.0072922926616692495 4 0.0 5 0.003777934752431057 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.785894773095483E-4 14-15 2.1964736932738707E-4 16-17 4.392947386547741E-5 18-19 0.0015375315852917092 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0018889673762155284 28-29 0.003865793700162012 30-31 0.002020755797811961 32-33 0.009532695828808597 34-35 0.002284332641004825 36-37 8.785894773095483E-4 38-39 0.001405743163695277 40-41 3.9536526478929667E-4 42-43 0.005886549497973972 44-45 0.0027675568535250766 46-47 3.0750631705834185E-4 48-49 6.150126341166837E-4 50-51 0.0 52-53 2.1964736932738707E-4 54-55 0.0025918389580631674 56-57 0.018670026392827898 58-59 0.03132171486608539 60-61 0.025347306420380463 62-63 0.01717642428140167 64-65 0.02218438430206609 66-67 0.0035582873831036706 68-69 0.0010103778989059805 70-71 1.3178842159643222E-4 72-73 4.392947386547741E-5 74-75 0.0016693200068881415 76-77 0.0025918389580631674 78-79 0.012036675839140809 80-81 0.0200318400826577 82-83 0.022404031671393477 84-85 0.03892151384481299 86-87 0.015375315852917093 88-89 0.05038710652370259 90-91 0.057284033920582546 92-93 0.03681289909927007 94-95 0.03821864226296535 96-97 0.062028417098054095 98-99 0.06686065922325661 100-101 0.07410902241106039 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1138188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.34433947621491 #Duplication Level Percentage of deduplicated Percentage of total 1 80.90060383627599 46.39191690218185 2 11.106881857046211 12.738336074653406 3 3.39993850710308 5.8490168384872305 4 1.4825977703333282 3.400743593946947 5 0.8203348794501153 2.352078090568362 6 0.46785765864322937 1.6097393042270651 7 0.3372373094528963 1.3537055530118556 8 0.21731875624916844 0.996960042632089 9 0.1710213840557171 0.8826397474484852 >10 0.9701966672771196 10.357198340623185 >50 0.07740875962508391 3.0131731799461114 >100 0.04137374762252187 4.436315215807459 >500 0.0030761135597955755 1.0981638828460212 >1k 0.003998947627734248 4.4495437897115835 >5k 0.0 0.0 >10k+ 1.5380567798977876E-4 1.0704694439082931 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12137 1.0663440486105986 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4433 0.3894787152913227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3916 0.344055639314419 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3416 0.30012616544894166 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3315 0.2912524117281152 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3081 0.2706934179590718 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2981 0.2619075231859763 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2256 0.19820978608103404 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2256 0.19820978608103404 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2100 0.1845037902350051 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1954 0.1716763838662857 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1792 0.15744323433387103 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1733 0.1522595564177447 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1602 0.1407500342649896 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1562 0.13723567635575143 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1448 0.1272197563144226 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1408 0.12370539840518438 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1198 0.10525501938168386 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1179 0.10358569937479574 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1170 0.10279496884521713 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1159 0.10182852042017662 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.392947386547741E-5 0.0 18-19 0.0 0.0 0.0 8.785894773095482E-5 0.0 20-21 0.0 0.0 0.0 8.785894773095482E-5 4.392947386547741E-5 22-23 0.0 0.0 0.0 1.7571789546190963E-4 8.785894773095482E-5 24-25 0.0 0.0 0.0 3.0750631705834185E-4 8.785894773095482E-5 26-27 0.0 0.0 0.0 5.710831602512063E-4 8.785894773095482E-5 28-29 0.0 0.0 0.0 0.0014496726375607546 8.785894773095482E-5 30-31 0.0 0.0 0.0 0.0031629221183143734 8.785894773095482E-5 32-33 0.0 0.0 0.0 0.0047004537036060825 8.785894773095482E-5 34-35 4.392947386547741E-5 0.0 0.0 0.006896927396879953 8.785894773095482E-5 36-37 8.785894773095482E-5 0.0 0.0 0.008829824246960959 8.785894773095482E-5 38-39 8.785894773095482E-5 0.0 0.0 0.011904887417544377 8.785894773095482E-5 40-41 8.785894773095482E-5 0.0 0.0 0.01440886742787659 8.785894773095482E-5 42-43 8.785894773095482E-5 0.0 0.0 0.021789019037276793 8.785894773095482E-5 44-45 8.785894773095482E-5 0.0 0.0 0.03180493907860564 8.785894773095482E-5 46-47 8.785894773095482E-5 0.0 0.0 0.04265551912337856 8.785894773095482E-5 48-49 8.785894773095482E-5 0.0 0.0 0.05579043180915631 8.785894773095482E-5 50-51 8.785894773095482E-5 0.0 0.0 0.07182468977005557 8.785894773095482E-5 52-53 8.785894773095482E-5 0.0 0.0 0.0987973867234587 8.785894773095482E-5 54-55 8.785894773095482E-5 0.0 0.0 0.12019104049594619 8.785894773095482E-5 56-57 8.785894773095482E-5 0.0 0.0 0.1656580459467153 8.785894773095482E-5 58-59 8.785894773095482E-5 0.0 0.0 0.22307386828889428 8.785894773095482E-5 60-61 8.785894773095482E-5 0.0 0.0 0.2794353832583018 8.785894773095482E-5 62-63 8.785894773095482E-5 0.0 0.0 0.3574980583172551 8.785894773095482E-5 64-65 8.785894773095482E-5 0.0 0.0 0.40779730589322677 8.785894773095482E-5 66-67 8.785894773095482E-5 0.0 0.0 0.5090547431531522 8.785894773095482E-5 68-69 8.785894773095482E-5 0.0 0.0 0.6285429120672508 8.785894773095482E-5 70-71 8.785894773095482E-5 0.0 0.0 0.7097245797706531 8.785894773095482E-5 72-73 8.785894773095482E-5 0.0 0.0 0.8541207603664773 8.785894773095482E-5 74-75 8.785894773095482E-5 0.0 0.0 0.9942997114712157 8.785894773095482E-5 76-77 8.785894773095482E-5 0.0 0.0 1.0862001707977944 8.785894773095482E-5 78-79 8.785894773095482E-5 0.0 0.0 1.1922459207090568 8.785894773095482E-5 80-81 8.785894773095482E-5 0.0 0.0 1.3857113236126195 8.785894773095482E-5 82-83 8.785894773095482E-5 0.0 0.0 1.5495243316569847 8.785894773095482E-5 84-85 1.3178842159643222E-4 0.0 0.0 1.7953097379343306 8.785894773095482E-5 86-87 1.7571789546190963E-4 0.0 0.0 2.146921246753612 8.785894773095482E-5 88-89 1.7571789546190963E-4 0.0 0.0 2.593332560174593 8.785894773095482E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 490 0.0 31.015055 4 TAAGGTG 625 0.0 28.87502 5 GTACATG 5350 0.0 24.77655 1 TACATGG 5545 0.0 23.815372 2 ACATGGG 5355 0.0 23.592773 3 GAGTACT 4805 0.0 22.78223 12-13 ATGGGTA 450 0.0 22.162842 5 GTACTTT 4995 0.0 21.915638 14-15 GGTAAGG 675 0.0 21.812967 3 AGGTAAG 620 0.0 21.452625 2 GTCCTAC 245 7.499693E-9 21.331287 1 GGGCGTA 245 7.537892E-9 21.321915 9 AGTACTT 5025 0.0 20.697922 12-13 CATGGGG 2485 0.0 20.449188 4 GTGTAGC 505 0.0 19.756882 1 GGTGTGC 1095 0.0 19.082626 8 GGACCGA 125 0.0060386527 18.995888 6 TACTTTT 5540 0.0 18.902426 14-15 GGGGCGC 405 1.8189894E-12 18.76137 6 TAGACTG 355 7.8216544E-11 18.729164 5 >>END_MODULE