FastQCFastQC Report
Thu 26 May 2016
SRR936683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922317
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91780.4774446670346254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77050.4008183873939626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.2260813383016433No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35870.18659773596134246No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27370.14238026298472103No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20440.10633001736966378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117350.026.190721
GTATCAA169400.025.4062751
TACATGG122050.025.103652
ACATGGG120250.024.176453
GAGTACT87250.023.49408512-13
GGTATCA123800.023.1378441
CATGGGG46100.022.1557464
AGTACTT90250.021.7393212-13
GTACTTT93950.021.66974314-15
TCAACGC199200.021.463574
ATCAACG199200.021.4386063
CAACGCA199950.021.3112325
TATCAAC201250.021.2904852
CATGGGA70100.021.2116834
TACCTGG16100.020.9482332
AACGCAG203650.020.8784716
GTGTACG2201.3447425E-619.4332771
CGCAGAG221100.019.1442248
ACGCAGA221750.019.1095317
GTACCTG18000.019.0014271
Produced by FastQC (version 0.11.5)