##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936683_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1922317 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.593414093513193 31.0 31.0 34.0 30.0 34.0 2 31.673367607943955 33.0 31.0 34.0 30.0 34.0 3 31.67807494809649 33.0 31.0 34.0 30.0 34.0 4 35.308175498630035 37.0 35.0 37.0 33.0 37.0 5 35.32351115866946 37.0 35.0 37.0 33.0 37.0 6 35.33552426576886 37.0 35.0 37.0 33.0 37.0 7 35.25986920991699 37.0 35.0 37.0 32.0 37.0 8 35.220095332871736 37.0 35.0 37.0 32.0 37.0 9 36.74238223976587 39.0 37.0 39.0 32.0 39.0 10-11 36.61970476253396 39.0 37.0 39.0 32.0 39.0 12-13 36.53677619248022 39.0 37.0 39.0 32.0 39.0 14-15 37.66026362977594 40.0 37.0 41.0 32.0 41.0 16-17 37.566033853937725 40.0 37.0 41.0 32.0 41.0 18-19 37.56684043266537 40.0 37.0 41.0 32.0 41.0 20-21 37.46408968968177 40.0 37.0 41.0 32.0 41.0 22-23 37.56711250017557 40.0 37.0 41.0 32.0 41.0 24-25 37.60669156023694 40.0 37.0 41.0 32.0 41.0 26-27 37.51980812737962 40.0 37.0 41.0 32.0 41.0 28-29 37.3871710545139 40.0 37.0 41.0 32.0 41.0 30-31 37.162913036715594 39.0 36.5 41.0 31.0 41.0 32-33 36.95652486036382 39.0 36.0 41.0 31.0 41.0 34-35 36.77172807606654 39.0 35.5 41.0 30.0 41.0 36-37 36.56411507571332 39.0 35.0 41.0 30.0 41.0 38-39 36.36494475156803 39.0 35.0 40.5 30.0 41.0 40-41 36.146309375612866 39.0 35.0 40.0 30.0 41.0 42-43 35.890882461113335 38.5 35.0 40.0 29.0 41.0 44-45 35.57736653215885 38.0 35.0 40.0 27.5 41.0 46-47 35.29939754993583 38.0 35.0 40.0 26.5 41.0 48-49 35.08347088435466 38.5 35.0 40.0 25.5 41.0 50-51 34.11767700124381 37.5 33.5 39.5 23.5 40.5 52-53 34.2177484254678 38.0 33.5 39.5 24.0 40.5 54-55 34.65277579088152 38.0 34.0 40.0 24.0 41.0 56-57 34.55999582795137 38.0 34.0 40.0 24.0 41.0 58-59 34.33447423083706 38.0 34.0 40.0 23.0 41.0 60-61 34.052352447593194 38.0 34.0 40.0 22.5 41.0 62-63 33.69499359366847 37.0 33.0 40.0 22.0 41.0 64-65 33.34365793987152 37.0 33.0 39.5 21.5 41.0 66-67 32.90561728372583 36.0 32.0 39.0 19.5 41.0 68-69 32.511390160935996 36.0 32.0 39.0 18.5 40.5 70-71 32.122660310448275 35.0 31.5 38.5 17.5 40.0 72-73 31.737495688796386 35.0 31.0 37.5 14.0 40.0 74-75 31.30776453623414 35.0 31.0 37.0 10.5 39.0 76-77 30.851030293130634 35.0 31.0 36.5 7.5 39.0 78-79 30.363631492620623 34.5 30.0 36.0 6.5 38.5 80-81 29.901680107911442 34.0 30.0 36.0 2.0 37.5 82-83 29.499398642367517 34.0 29.0 35.0 2.0 37.0 84-85 29.143449285419628 34.0 29.0 35.0 2.0 36.5 86-87 28.785942953217393 34.0 29.0 35.0 2.0 36.0 88-89 28.387012391816754 34.0 29.0 35.0 2.0 36.0 90-91 27.988032931093052 34.0 27.5 35.0 2.0 35.5 92-93 27.557248882468397 34.0 26.5 35.0 2.0 35.0 94-95 27.087110762688983 33.0 26.0 35.0 2.0 35.0 96-97 26.526101054092535 33.0 25.0 35.0 2.0 35.0 98-99 25.805577331938487 33.0 23.5 35.0 2.0 35.0 100-101 22.92692204251432 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4793315224739949 1101 2 0.3844733695523388 1101 3 0.37346310275809813 1101 4 0.41562341978934114 1101 5 0.39436832852745596 1101 6 0.5646674078406093 1101 7 0.48008215211110894 1101 8 0.5253464786654618 1101 9 0.46416624091400394 1101 10-11 0.7067590420339158 1101 12-13 0.6941817813971483 1101 14-15 0.7424562192400899 1101 16-17 0.7317642582496049 1101 18-19 0.6683414439722171 1101 20-21 0.7502216517442619 1101 22-23 0.5159911673465345 1101 24-25 0.6226921707008088 1101 26-27 0.5855192829160814 1101 28-29 0.489416886631453 1101 30-31 0.6860075738060232 1101 32-33 0.6188950542800384 1101 34-35 0.6241139379006952 1101 36-37 0.47221361799820016 1101 38-39 0.3394105248816075 1101 40-41 0.4256232053851292 1101 42-43 0.4611531421218231 1101 44-45 0.24318700206514876 1101 46-47 0.3210112429583276 1101 48-49 0.5051549071707058 1101 50-51 0.4078073093775174 1101 52-53 0.5123189590670947 1101 54-55 0.3909525546982735 1101 56-57 0.42311087530210045 1101 58-59 0.4810732897059111 1101 60-61 0.5997216143465423 1101 62-63 0.5162032331654913 1101 64-65 0.45704039823515075 1101 66-67 0.5113954822501867 1101 68-69 0.2420407934416957 1101 70-71 0.24964767679563948 1101 72-73 0.18088839199734608 1101 74-75 0.29148617728864323 1101 76-77 0.34649357141631754 1101 78-79 0.2910905221437794 1101 80-81 0.3923153473527847 1101 82-83 0.33115021070001305 1101 84-85 0.29905066900772326 1101 86-87 0.36971681320720506 1101 88-89 0.34308385169877553 1101 90-91 0.5251675942193934 1101 92-93 0.18814925049910514 1101 94-95 0.32454919014359973 1101 96-97 0.1569643268107086 1101 98-99 0.29444480890747116 1101 100-101 0.7388664604259461 1102 1 0.2256190262622546 1102 2 0.4411063720518982 1102 3 0.4894605948595121 1102 4 0.4132640395906222 1102 5 0.30447601246549993 1102 6 0.4246402899128441 1102 7 0.4148095965476273 1102 8 0.46391269469506824 1102 9 0.5641281497714417 1102 10-11 0.5859432752666294 1102 12-13 0.552095563810532 1102 14-15 0.6200549434185092 1102 16-17 0.4428429419457629 1102 18-19 0.4644794562447885 1102 20-21 0.38240660509419655 1102 22-23 0.33488713126097736 1102 24-25 0.39309966251492057 1102 26-27 0.19022184762699368 1102 28-29 0.1465771409801917 1102 30-31 0.5809038240991242 1102 32-33 0.4373473166251358 1102 34-35 0.3946430711372102 1102 36-37 0.301023705385397 1102 38-39 0.24871410109309267 1102 40-41 0.312585191166292 1102 42-43 0.38603435058417546 1102 44-45 0.48760505096453954 1102 46-47 0.4471071742496875 1102 48-49 0.6446127432416233 1102 50-51 0.6490056835993627 1102 52-53 0.7855403066038207 1102 54-55 0.7924022655855794 1102 56-57 0.7408698973884711 1102 58-59 0.6529175173222725 1102 60-61 0.8110265510883679 1102 62-63 0.8077887283179024 1102 64-65 0.8170593881989205 1102 66-67 0.7811155262542613 1102 68-69 0.6074033173181732 1102 70-71 0.6123696550141773 1102 72-73 0.33631253362595714 1102 74-75 0.5863788130937628 1102 76-77 0.6729156639082845 1102 78-79 0.6382278013240494 1102 80-81 0.7359352199930598 1102 82-83 0.613223230771542 1102 84-85 0.17059767449710606 1102 86-87 0.4253812024375101 1102 88-89 0.434967727467896 1102 90-91 0.2882531991966353 1102 92-93 0.3365335975418553 1102 94-95 0.30836728964581894 1102 96-97 0.36605979192580307 1102 98-99 0.4564067715188358 1102 100-101 0.4335248264364111 1103 1 0.32146417546744743 1103 2 0.2422817972025051 1103 3 0.3616405686185509 1103 4 0.42341600278167135 1103 5 0.4222710556112901 1103 6 0.4186631554001039 1103 7 0.4738481805954393 1103 8 0.4488039727616666 1103 9 0.16781611002953412 1103 10-11 0.4580888784733972 1103 12-13 0.21074537872272003 1103 14-15 0.5685303826753696 1103 16-17 0.5294668777415623 1103 18-19 0.6521777582201693 1103 20-21 0.548828341795236 1103 22-23 0.42431262509301604 1103 24-25 0.4733081199144138 1103 26-27 0.3054584327885195 1103 28-29 0.3769682038728064 1103 30-31 0.3671604007491567 1103 32-33 0.2369943262796852 1103 34-35 0.4685839151204405 1103 36-37 0.4862220270522357 1103 38-39 0.46159121944689474 1103 40-41 0.24555151542428888 1103 42-43 0.3167053663986863 1103 44-45 0.42228417441043575 1103 46-47 0.5495684160816126 1103 48-49 0.5068031785375098 1103 50-51 0.4718717914422186 1103 52-53 0.5732842450347064 1103 54-55 0.5725580692084691 1103 56-57 0.675965167069613 1103 58-59 0.4100155734049906 1103 60-61 0.6326264382383897 1103 62-63 0.6386142445385374 1103 64-65 0.5684537227384894 1103 66-67 0.6833603052122257 1103 68-69 0.6322310289578326 1103 70-71 0.5452684315895979 1103 72-73 0.5213533235949086 1103 74-75 0.46486089016351784 1103 76-77 0.4895115328065849 1103 78-79 0.472017538993196 1103 80-81 0.512960319804904 1103 82-83 0.42461637666284346 1103 84-85 0.4291470510176758 1103 86-87 0.5152218877672254 1103 88-89 0.399978863288144 1103 90-91 0.5263032861612942 1103 92-93 0.5762644999860527 1103 94-95 0.6687913411080828 1103 96-97 0.6920888259724585 1103 98-99 0.6977231350284825 1103 100-101 0.5328276770679601 1104 1 0.3194041597367736 1104 2 0.3529617841091621 1104 3 0.3422959674485462 1104 4 0.4110406835823639 1104 5 0.35077262192555025 1104 6 0.47927142605999506 1104 7 0.5174262675679344 1104 8 0.5799421824068816 1104 9 0.6181843172756132 1104 10-11 0.6831482368358408 1104 12-13 0.6627514814180273 1104 14-15 0.6307398373347581 1104 16-17 0.512027956876473 1104 18-19 0.48233622644658425 1104 20-21 0.36687141770887166 1104 22-23 0.43445505323986566 1104 24-25 0.6449126607401254 1104 26-27 0.5189928515529516 1104 28-29 0.625513067597204 1104 30-31 0.6658478252236364 1104 32-33 0.5085178563544659 1104 34-35 0.6017735458425477 1104 36-37 0.5215492463944358 1104 38-39 0.4627781411341303 1104 40-41 0.4608472243101005 1104 42-43 0.6599554568267223 1104 44-45 0.6786822188565864 1104 46-47 0.4761288184489274 1104 48-49 0.5063784798599329 1104 50-51 0.44388244193718407 1104 52-53 0.4542059307950055 1104 54-55 0.4794758814810933 1104 56-57 0.571496536450887 1104 58-59 0.5107514231056101 1104 60-61 0.6058353062512722 1104 62-63 0.5248784898702255 1104 64-65 0.5266667616140737 1104 66-67 0.6404567757808195 1104 68-69 0.6622047612402611 1104 70-71 0.6794310999005404 1104 72-73 0.5631048010772872 1104 74-75 0.6035465564173954 1104 76-77 0.4598884623984496 1104 78-79 0.4674408858545185 1104 80-81 0.6498226916494048 1104 82-83 0.40258201720151376 1104 84-85 0.25851198713852597 1104 86-87 0.5074350086603658 1104 88-89 0.6055011002358732 1104 90-91 0.4504941424810305 1104 92-93 0.17116666704205485 1104 94-95 0.1652040554004266 1104 96-97 0.40516281685574285 1104 98-99 0.5972477033374375 1104 100-101 0.6566226145222096 1105 1 0.3543138759046265 1105 2 0.3263556204225182 1105 3 0.4424111708911518 1105 4 0.35704218526807097 1105 5 0.4060893902572573 1105 6 0.5336103806728403 1105 7 0.4791243290480054 1105 8 0.6032396044969559 1105 9 0.7202887824313535 1105 10-11 0.6243658072845264 1105 12-13 0.5489505155093894 1105 14-15 0.5569946317313352 1105 16-17 0.5975689932331889 1105 18-19 0.5830949576262157 1105 20-21 0.6035472237034654 1105 22-23 0.3128902560823761 1105 24-25 0.342368118917598 1105 26-27 0.39092635538060705 1105 28-29 0.3316869413147785 1105 30-31 0.2559702612453165 1105 32-33 0.13069593459843531 1105 34-35 0.2833271299617479 1105 36-37 0.36136032339538104 1105 38-39 0.17972671868254508 1105 40-41 0.3626139674644975 1105 42-43 0.2587963083885043 1105 44-45 0.1858556354867673 1105 46-47 0.34304222519208594 1105 48-49 0.31272853426760605 1105 50-51 0.31406744739005177 1105 52-53 0.37241426924002496 1105 54-55 0.3546444460645901 1105 56-57 0.26115077851942914 1105 58-59 0.25811893612196 1105 60-61 0.2228805278975159 1105 62-63 0.28953982780299015 1105 64-65 0.17213197808693081 1105 66-67 0.41745402865903003 1105 68-69 0.4522449747670976 1105 70-71 0.3201304121401094 1105 72-73 0.21807170035280166 1105 74-75 0.35890729128033527 1105 76-77 0.19452552315961213 1105 78-79 0.1837264568229564 1105 80-81 0.2867002790991293 1105 82-83 0.39444857188298243 1105 84-85 0.27155579469336644 1105 86-87 0.4611668283061192 1105 88-89 0.4707782841710788 1105 90-91 0.16771167324610659 1105 92-93 0.2622780475500015 1105 94-95 0.3771028941468231 1105 96-97 0.6885555425120877 1105 98-99 0.6825118013617484 1105 100-101 0.6516305863294285 1106 1 0.3692524675970503 1106 2 0.332831843459207 1106 3 0.43617412587538595 1106 4 0.36071414635548393 1106 5 0.27137691505751604 1106 6 0.3473990762176342 1106 7 0.4729316724507413 1106 8 0.5142790750092345 1106 9 0.5748132962896335 1106 10-11 0.6385649068135066 1106 12-13 0.5970502071751582 1106 14-15 0.568331386593222 1106 16-17 0.5485740957763383 1106 18-19 0.4268088846577953 1106 20-21 0.34432865820020453 1106 22-23 0.5063936568727243 1106 24-25 0.3759936445248755 1106 26-27 0.4771117823322655 1106 28-29 0.4929239757056507 1106 30-31 0.5607953295574362 1106 32-33 0.4152262874226125 1106 34-35 0.4228045130000382 1106 36-37 0.3384010042003922 1106 38-39 0.3837642231184404 1106 40-41 0.4125117442422237 1106 42-43 0.37397646279881513 1106 44-45 0.47099137078446773 1106 46-47 0.5145881156155028 1106 48-49 0.49222231268770145 1106 50-51 0.2813096209209007 1106 52-53 0.4549219504433495 1106 54-55 0.510996157226316 1106 56-57 0.4344147132363432 1106 58-59 0.41989193705529004 1106 60-61 0.5915320101047286 1106 62-63 0.6416681934426691 1106 64-65 0.6535087934965063 1106 66-67 0.7313289038461193 1106 68-69 0.7025904606892368 1106 70-71 0.5805191319096465 1106 72-73 0.39790063494339734 1106 74-75 0.570679123476058 1106 76-77 0.6012541226884807 1106 78-79 0.49303121377393566 1106 80-81 0.7514402916459382 1106 82-83 0.5561823787872235 1106 84-85 0.6683392635018812 1106 86-87 0.8039761052022563 1106 88-89 0.5949061284829504 1106 90-91 0.6106053825808253 1106 92-93 0.5076012672497718 1106 94-95 0.464906137287457 1106 96-97 0.5977253870038766 1106 98-99 0.7869272920698123 1106 100-101 0.4934205823604394 1107 1 0.193240099146486 1107 2 0.1416212121128524 1107 3 0.23497766340893023 1107 4 0.2407822838157685 1107 5 0.1877292293739572 1107 6 0.2877228410687849 1107 7 0.4057158771690226 1107 8 0.41393393443141946 1107 9 0.6021062848041439 1107 10-11 0.5361316226061135 1107 12-13 0.3701612544015731 1107 14-15 0.21539197936395027 1107 16-17 0.3525960105592816 1107 18-19 0.3354339483967692 1107 20-21 0.3134093970337304 1107 22-23 0.3554172119069392 1107 24-25 0.3884290108138444 1107 26-27 0.3831732977006155 1107 28-29 0.4209814122458724 1107 30-31 0.4096598723143714 1107 32-33 0.15771080062937415 1107 34-35 0.21638570963504122 1107 36-37 0.3113126099282866 1107 38-39 0.24818095932382533 1107 40-41 0.4500799102641011 1107 42-43 0.2763063190792181 1107 44-45 0.4259282939715092 1107 46-47 0.39674008332248434 1107 48-49 0.3237284037672339 1107 50-51 0.08385752453521178 1107 52-53 0.37359498133159974 1107 54-55 0.41146951019603506 1107 56-57 0.18445673957503317 1107 58-59 0.3490366958171194 1107 60-61 0.30444513600863843 1107 62-63 0.3670511926986677 1107 64-65 0.41273272964725294 1107 66-67 0.49886915878337135 1107 68-69 0.5336317556180745 1107 70-71 0.39698130925403063 1107 72-73 0.2167568563686899 1107 74-75 0.5411111169327718 1107 76-77 0.2620632153928746 1107 78-79 0.0076556801676694874 1107 80-81 0.20130945625092522 1107 82-83 0.21840614450105278 1107 84-85 -0.033270001364773094 1107 86-87 -0.08618266692452181 1107 88-89 -0.06284512548740295 1107 90-91 -0.1319067595920025 1107 92-93 -0.038845239691159605 1107 94-95 0.03975755290474581 1107 96-97 0.05729301816627341 1107 98-99 -0.17532186431409613 1107 100-101 -0.4022447476238433 1108 1 0.0807652828217158 1108 2 0.0830322196640374 1108 3 0.09559032412030888 1108 4 0.24714426303066261 1108 5 0.18529075161465158 1108 6 0.17527651440725833 1108 7 0.21372142505610725 1108 8 0.07773074235458921 1108 9 0.19122313848170336 1108 10-11 0.26657192560317355 1108 12-13 0.024138031527407122 1108 14-15 0.29038710050911476 1108 16-17 0.3963875906292387 1108 18-19 0.266697650144188 1108 20-21 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-2.0334439287526713 2316 62-63 -1.8057741871751674 2316 64-65 -2.019327541620065 2316 66-67 -1.9474971686402291 2316 68-69 -1.8309337653351108 2316 70-71 -1.7013557428275163 2316 72-73 -1.7867118563511255 2316 74-75 -1.70061281421847 2316 76-77 -1.7299949793084934 2316 78-79 -2.4743862622266946 2316 80-81 -2.2823375868674844 2316 82-83 -2.4272490650308107 2316 84-85 -2.0735564403296287 2316 86-87 -2.0382044581533627 2316 88-89 -2.453774558267053 2316 90-91 -2.454820181624367 2316 92-93 -3.21854235771427 2316 94-95 -2.825922533039659 2316 96-97 -3.1611278169946537 2316 98-99 -4.465685115029771 2316 100-101 -5.536872968155148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3586.0 3 1395.0 4 1325.0 5 1450.0 6 1726.0 7 2119.0 8 2569.0 9 3337.0 10 4274.0 11 5571.0 12 7516.0 13 9288.0 14 11076.0 15 13939.0 16 17906.0 17 23889.0 18 18273.0 19 12242.0 20 11877.0 21 12475.0 22 13783.0 23 15353.0 24 17525.0 25 20203.0 26 23554.0 27 27954.0 28 33189.0 29 40247.0 30 49121.0 31 61571.0 32 77142.0 33 100317.0 34 134943.0 35 187412.0 36 266324.0 37 358104.0 38 288288.0 39 41440.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72598492053417 18.735445485212924 14.653417852705708 21.885151741547197 2 15.37090450504445 20.124165501281922 40.71188359471248 23.793046398961142 3 19.273578068427945 25.609101943704925 29.951442495542523 25.165877492324608 4 12.36728322797159 16.764483987616742 37.89916491063764 32.96906787377403 5 13.138957332160764 37.894213732076466 34.071098321542586 14.895730614220184 6 30.65287743012654 36.394518169544945 17.970367471252764 14.982236929075748 7 28.205473693848543 33.531108951562054 20.68840481695047 17.57501253763893 8 26.71719857510217 33.599729839704196 19.932595214333066 19.750476370860568 9 26.992021298740227 16.874859493259837 20.736088791933454 35.39703041606648 10-11 24.77056533212179 26.78626491107414 27.14038625221277 21.302783504591297 12-13 26.331470254130522 24.4958316182979 27.695112057943653 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21.555477466899962 26.386133604950068 30.401783473220984 21.656605454928986 40-41 21.586078511903352 26.492974931780743 30.469706904951348 21.451239651364553 42-43 21.513204920514525 26.56614321431581 29.86258725506415 22.058064610105514 44-45 21.631555619831015 26.661774911277327 29.747430397829245 21.959239071062413 46-47 22.013085903716775 26.937999971388404 29.16382609871781 21.88508802617701 48-49 22.08599951672513 27.091639891036863 28.764426392009785 22.057934200228217 50-51 21.900688543684975 27.32161422669477 28.780064773204046 21.99763245641621 52-53 22.163208793003843 27.4715301894051 28.455374428633444 21.909886588957608 54-55 21.692405895658577 27.27228507752462 28.678777959742547 22.35653106707425 56-57 22.02706857347671 27.619371139721473 28.215401005980844 22.138159280820975 58-59 22.185170175541728 27.100864052572522 28.367003585956034 22.346962185929716 60-61 21.866904094538324 27.20619601429145 29.02860059055241 21.89829930061781 62-63 21.858776013001037 27.344109268649973 28.867096219752508 21.93001849859648 64-65 21.662524072650243 27.4912006725256 28.691373797928115 22.154901456896038 66-67 21.638583867283735 27.602100941989967 28.29437641617174 22.464938774554565 68-69 21.69050661073799 27.426899367419637 28.65105035402122 22.23154366782115 70-71 21.80272485917432 27.422532213077595 28.404577621845505 22.370165305902578 72-73 21.81211920827839 27.29427134209076 28.516967042469176 22.376642407161672 74-75 21.580430387848608 28.0242018363423 28.239968154109135 22.155399621699956 76-77 21.763887118301 27.63377188988649 28.25039610580513 22.351944886007384 78-79 21.8748780098071 27.622301186564446 28.2015821900304 22.30123861359805 80-81 21.86918773172607 27.92689723499701 27.991090057114953 22.212824976161972 82-83 21.864972938481692 27.85332599826269 28.110747422532857 22.170953640722757 84-85 21.65547978543873 27.766297938805966 28.0486126593646 22.529609616390694 86-87 21.911195956953396 27.657359813144893 28.065676093192373 22.365768136709338 88-89 21.825766730709944 27.588022649726636 27.900035251986665 22.68617536757676 90-91 21.690864421624305 27.519896211328387 28.256158809573495 22.53308055747381 92-93 21.86018582813928 27.522108490893704 28.28284194825816 22.33486373270886 94-95 21.482709517712422 27.292438512873368 28.373044880282656 22.851807089131558 96-97 21.48579536364041 27.166505242953743 28.448789660349423 22.898909733056428 98-99 21.327667406006164 27.501113173072845 28.058909821660695 23.112309599260296 100-101 21.408441979125538 27.368119942143498 28.207544198615324 23.01589388011564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 258.0 1 217.0 2 274.0 3 375.0 4 346.5 5 343.0 6 543.5 7 1114.0 8 1894.0 9 2410.0 10 2304.5 11 2106.0 12 2190.0 13 2354.5 14 2550.5 15 2785.0 16 2992.5 17 3156.5 18 3409.0 19 3728.5 20 4162.0 21 4614.0 22 5245.0 23 6263.0 24 7375.0 25 8957.5 26 11502.5 27 14432.0 28 16810.0 29 19884.0 30 24184.0 31 28871.0 32 34680.0 33 39854.0 34 44556.0 35 48503.5 36 53268.5 37 58082.0 38 58971.5 39 58613.0 40 58878.0 41 60832.5 42 62500.5 43 64286.0 44 67095.0 45 69994.5 46 69914.0 47 71197.0 48 81160.5 49 79512.5 50 67428.5 51 61025.0 52 57367.5 53 56749.5 54 55831.0 55 52473.5 56 47729.5 57 42634.5 58 39064.0 59 35033.0 60 29349.0 61 24589.5 62 20799.5 63 16027.5 64 11574.5 65 8856.0 66 6571.5 67 4985.5 68 3656.0 69 2445.0 70 1851.5 71 1506.5 72 1246.0 73 1124.0 74 897.5 75 699.0 76 526.5 77 300.5 78 178.5 79 123.0 80 83.0 81 51.5 82 32.0 83 22.0 84 15.0 85 11.5 86 9.5 87 6.0 88 3.5 89 2.5 90 3.5 91 2.0 92 1.5 93 3.0 94 1.5 95 0.5 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012120789651238584 2 0.01066421407083223 3 0.013837468013860356 4 0.019091544214611848 5 0.03183658054316744 6 0.0475988091454219 7 0.005462158426523825 8 0.025750175439326606 9 0.03646641006660192 10-11 0.04164245543268878 12-13 0.030588087188533418 14-15 0.02751887435839146 16-17 0.030093891902324123 18-19 0.0249438568144588 20-21 0.05339910118882578 22-23 0.012927108276106387 24-25 0.0032772950559142953 26-27 7.282877902031768E-4 28-29 2.6010278221542027E-5 30-31 0.0023929455963818663 32-33 0.002132842814166446 34-35 0.0 36-37 3.1212333865850427E-4 38-39 2.6010278221542027E-5 40-41 0.001170462519969391 42-43 0.004109623959003639 44-45 0.0018207194755079418 46-47 0.001014400850640139 48-49 8.063186248678028E-4 50-51 0.0037194697856805094 52-53 0.003199264221249669 54-55 0.00462982952343448 56-57 7.282877902031768E-4 58-59 0.004993973418536068 60-61 0.0025490072657111183 62-63 4.6818500798775643E-4 64-65 5.722261208739246E-4 66-67 0.016750619174673062 68-69 0.04276089739621509 70-71 0.05045993974979152 72-73 0.013135190501878722 74-75 0.029235552721013237 76-77 0.02507390820556651 78-79 0.05493370760389676 80-81 0.08018968775701407 82-83 0.04962761084670218 84-85 0.01812916392041479 86-87 0.02080822257723362 88-89 0.02286303455673544 90-91 0.037454800639020513 92-93 0.025360021266003476 94-95 0.012901097997884844 96-97 0.018545328371959463 98-99 0.05241071061640718 100-101 0.07061790537148659 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1922317.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.516376372806405 #Duplication Level Percentage of deduplicated Percentage of total 1 76.99744669451043 40.43626890354008 2 13.037885539039221 13.694050081475073 3 4.230880574899547 6.665715499794603 4 1.8007903832128171 3.782839421333383 5 0.9829433240515344 2.581031077951389 6 0.5949619789099403 1.8747148327166476 7 0.3934627470384738 1.446426639850556 8 0.2975838117761879 1.250241876935413 9 0.21241687859650543 1.0039828269879723 >10 1.2549411231603615 12.554985283025422 >50 0.12306972032810003 4.496824518685686 >100 0.06793993768775652 6.519932422880609 >500 0.004183263949876039 1.4936818471557225 >1k 0.00129481979400925 1.3162038212627625 >5k 1.9920304523219235E-4 0.8831009464046108 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9178 0.4774446670346254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7705 0.4008183873939626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4346 0.2260813383016433 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3587 0.18659773596134246 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2737 0.14238026298472103 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2044 0.10633001736966378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.202055644308405E-5 0.0 0.0 0.0 6 0.0 5.202055644308405E-5 0.0 0.0 0.0 7 0.0 5.202055644308405E-5 0.0 0.0 0.0 8 0.0 5.202055644308405E-5 0.0 0.0 5.202055644308405E-5 9 0.0 5.202055644308405E-5 0.0 0.0 5.202055644308405E-5 10-11 0.0 5.202055644308405E-5 0.0 0.0 5.202055644308405E-5 12-13 0.0 5.202055644308405E-5 0.0 0.0 5.202055644308405E-5 14-15 0.0 5.202055644308405E-5 0.0 0.0 1.5606166932925213E-4 16-17 0.0 5.202055644308405E-5 0.0 0.0 2.080822257723362E-4 18-19 0.0 5.202055644308405E-5 0.0 2.6010278221542023E-5 2.080822257723362E-4 20-21 0.0 5.202055644308405E-5 0.0 5.202055644308405E-5 2.3409250399387824E-4 22-23 0.0 5.202055644308405E-5 0.0 1.040411128861681E-4 3.901541733231304E-4 24-25 0.0 5.202055644308405E-5 0.0 2.080822257723362E-4 5.202055644308405E-4 26-27 0.0 5.202055644308405E-5 0.0 3.121233386585043E-4 5.722261208739246E-4 28-29 0.0 5.202055644308405E-5 0.0 9.623802941970548E-4 5.982363990954666E-4 30-31 0.0 5.202055644308405E-5 0.0 0.001872740031951026 6.242466773170085E-4 32-33 0.0 5.202055644308405E-5 0.0 0.0028351203261480808 6.242466773170085E-4 34-35 0.0 5.202055644308405E-5 0.0 0.0041876547936682655 6.242466773170085E-4 36-37 0.0 5.202055644308405E-5 0.0 0.00603438454739775 6.502569555385505E-4 38-39 0.0 5.202055644308405E-5 0.0 0.007412929293139477 6.762672337600926E-4 40-41 0.0 5.202055644308405E-5 0.0 0.00949375155086284 6.762672337600926E-4 42-43 0.0 5.202055644308405E-5 0.0 0.013265241892986432 6.762672337600926E-4 44-45 0.0 5.202055644308405E-5 0.0 0.019481698387934974 6.762672337600926E-4 46-47 0.0 5.202055644308405E-5 0.0 0.035295947546632525 7.282877902031766E-4 48-49 0.0 5.202055644308405E-5 0.0 0.0664042402995968 7.803083466462607E-4 50-51 0.0 5.202055644308405E-5 0.0 0.08918924402166761 7.803083466462607E-4 52-53 0.0 5.202055644308405E-5 0.0 0.10999746659890122 7.803083466462607E-4 54-55 0.0 5.202055644308405E-5 0.0 0.13842670069504664 7.803083466462607E-4 56-57 0.0 7.803083466462607E-5 0.0 0.16305843417084698 7.803083466462607E-4 58-59 0.0 1.040411128861681E-4 0.0 0.19507708666156517 7.803083466462607E-4 60-61 0.0 1.040411128861681E-4 0.0 0.2498547325961327 7.803083466462607E-4 62-63 0.0 1.040411128861681E-4 0.0 0.2915752188634861 7.803083466462607E-4 64-65 0.0 1.040411128861681E-4 0.0 0.3389399355049141 7.803083466462607E-4 66-67 0.0 1.040411128861681E-4 0.0 0.4283632720305756 8.063186248678028E-4 68-69 0.0 1.040411128861681E-4 0.0 0.5331586829851684 8.323289030893448E-4 70-71 0.0 1.040411128861681E-4 0.0 0.6155852546692351 8.323289030893448E-4 72-73 0.0 1.040411128861681E-4 0.0 0.7103927187867558 8.323289030893448E-4 74-75 0.0 1.040411128861681E-4 0.0 0.7947180407809951 8.323289030893448E-4 76-77 0.0 1.040411128861681E-4 0.0 0.8593795924397485 8.323289030893448E-4 78-79 0.0 1.040411128861681E-4 0.0 0.9507797101102471 8.323289030893448E-4 80-81 0.0 1.040411128861681E-4 0.0 1.0839263243263209 8.583391813108867E-4 82-83 0.0 1.040411128861681E-4 0.0 1.2338755782735107 9.363700159755129E-4 84-85 0.0 1.040411128861681E-4 0.0 1.412410127986175 0.001014400850640139 86-87 0.0 1.040411128861681E-4 0.0 1.691500413303321 0.001092431685304765 88-89 0.0 1.040411128861681E-4 0.0 2.0661004402499694 0.001092431685304765 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11735 0.0 26.19072 1 GTATCAA 16940 0.0 25.406275 1 TACATGG 12205 0.0 25.10365 2 ACATGGG 12025 0.0 24.17645 3 GAGTACT 8725 0.0 23.494085 12-13 GGTATCA 12380 0.0 23.137844 1 CATGGGG 4610 0.0 22.155746 4 AGTACTT 9025 0.0 21.73932 12-13 GTACTTT 9395 0.0 21.669743 14-15 TCAACGC 19920 0.0 21.46357 4 ATCAACG 19920 0.0 21.438606 3 CAACGCA 19995 0.0 21.311232 5 TATCAAC 20125 0.0 21.290485 2 CATGGGA 7010 0.0 21.211683 4 TACCTGG 1610 0.0 20.948233 2 AACGCAG 20365 0.0 20.878471 6 GTGTACG 220 1.3447425E-6 19.433277 1 CGCAGAG 22110 0.0 19.144224 8 ACGCAGA 22175 0.0 19.109531 7 GTACCTG 1800 0.0 19.001427 1 >>END_MODULE