FastQCFastQC Report
Thu 26 May 2016
SRR936683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922317
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106000.5514178982966909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93760.48774473721035605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55140.28684134822716545No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48990.25484870601466875No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28720.1494030381045374No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19510.10149210562045699No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19330.10055573560448147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117250.024.9197331
GTATCAA193950.024.6672551
TCGCGTA1351.3442204E-524.6275489
GGTATCA143450.023.4815541
TACATGG122150.023.3372782
GAGTACT111000.022.85149812-13
ACATGGG120750.022.5049863
TAAGGTG11200.022.4792865
GTAAGGT9100.022.446664
TACCTGG16850.021.9931352
GTACTTT115750.021.89351514-15
AGTACTT114800.021.66063512-13
CGCGTAA804.9934468E-520.78111310-11
ATCAACG232250.020.4966013
CATGGGA65850.020.487424
TCAACGC232700.020.4344224
TATCAAC233050.020.4273032
CAACGCA233350.020.4182175
AACGCAG237050.020.0974256
GTACCTG19150.019.599251
Produced by FastQC (version 0.11.5)