##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936683_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1922317 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.995247922168925 31.0 31.0 34.0 30.0 34.0 2 32.112291573138044 33.0 31.0 34.0 30.0 34.0 3 32.22229580240928 34.0 31.0 34.0 30.0 34.0 4 35.9515745842127 37.0 35.0 37.0 35.0 37.0 5 35.83158084748769 37.0 35.0 37.0 35.0 37.0 6 35.890807811614835 37.0 35.0 37.0 35.0 37.0 7 35.8229079803175 37.0 35.0 37.0 35.0 37.0 8 35.79430655817953 37.0 35.0 37.0 33.0 37.0 9 37.41004319266801 39.0 37.0 39.0 34.0 39.0 10-11 37.31367251082938 39.0 37.0 39.0 34.0 39.0 12-13 37.192355371148466 39.0 37.0 39.0 33.0 39.0 14-15 38.28566308262373 40.0 38.0 41.0 33.0 41.0 16-17 38.27146901369545 40.0 37.5 41.0 33.5 41.0 18-19 38.44548661849217 40.0 38.0 41.0 34.0 41.0 20-21 38.473358192223245 40.0 38.0 41.0 34.0 41.0 22-23 38.378689622991416 40.0 38.0 41.0 34.0 41.0 24-25 38.26884197559508 40.0 38.0 41.0 34.0 41.0 26-27 38.06779865131505 40.0 37.0 41.0 33.0 41.0 28-29 38.075344753232685 40.0 37.0 41.0 33.0 41.0 30-31 37.930027409631194 40.0 37.0 41.0 33.0 41.0 32-33 37.7124951295754 40.0 36.5 41.0 33.0 41.0 34-35 37.52533531150169 40.0 36.0 41.0 33.0 41.0 36-37 37.33042573103188 39.5 36.0 41.0 32.0 41.0 38-39 37.118279659390204 39.0 36.0 41.0 31.5 41.0 40-41 36.87753710756343 39.0 35.5 41.0 31.0 41.0 42-43 36.588267179658715 39.0 35.0 41.0 30.5 41.0 44-45 36.24048765110021 39.0 35.0 40.0 30.0 41.0 46-47 35.76963632949196 39.0 35.0 40.0 28.5 41.0 48-49 35.299986942840334 38.0 35.0 40.0 27.0 41.0 50-51 34.970423192428726 38.0 34.5 40.0 26.0 41.0 52-53 34.70055199012442 38.0 34.0 40.0 25.5 41.0 54-55 34.371403623856 38.0 34.0 40.0 24.0 41.0 56-57 34.04153581329198 38.0 33.0 40.0 23.0 41.0 58-59 33.688261873561956 37.0 33.0 40.0 22.5 41.0 60-61 33.32213027299868 37.0 32.5 39.5 22.0 41.0 62-63 33.00415332122641 36.0 32.0 39.0 21.0 40.0 64-65 32.693081057910845 36.0 32.0 39.0 20.0 40.0 66-67 32.250411872755635 35.5 31.0 39.0 18.5 40.0 68-69 31.809637796471655 35.0 31.0 38.0 17.5 40.0 70-71 31.31267111511785 35.0 30.5 37.5 14.0 39.5 72-73 30.764987252362644 34.0 30.0 37.0 9.5 39.0 74-75 30.230155328179485 34.0 29.0 36.0 7.5 39.0 76-77 28.85218358886698 32.5 28.0 35.0 7.0 37.0 78-79 29.61891326976768 34.0 29.0 35.0 6.5 37.0 80-81 29.464303234065973 34.0 29.0 35.0 2.0 37.0 82-83 29.0686564702908 34.0 29.0 35.0 2.0 36.5 84-85 28.631657785890674 33.5 29.0 35.0 2.0 36.0 86-87 28.212810894353012 33.0 27.5 35.0 2.0 36.0 88-89 27.724908534856635 33.0 26.5 35.0 2.0 35.0 90-91 27.16348890427541 32.5 26.0 34.5 2.0 35.0 92-93 26.596120983167708 32.0 25.0 34.0 2.0 35.0 94-95 25.87612787068938 32.0 24.0 34.0 2.0 35.0 96-97 25.077776194040837 31.5 20.0 34.0 2.0 35.0 98-99 23.9842648220871 31.0 8.5 34.0 2.0 35.0 100-101 20.217178540271973 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19479026640705754 1101 2 0.31501835870949435 1101 3 0.20104569894321145 1101 4 0.17939029423018837 1101 5 0.15612916959541678 1101 6 0.175654725892052 1101 7 0.13070619135224604 1101 8 0.10152260717757144 1101 9 0.284411386695389 1101 10-11 0.21261021842061467 1101 12-13 0.2885948167921626 1101 14-15 0.3212363538965093 1101 16-17 0.32767974755724083 1101 18-19 0.17935432359725212 1101 20-21 0.11048766122159748 1101 22-23 0.22265692601487075 1101 24-25 0.21535699324160618 1101 26-27 0.06264195536137862 1101 28-29 0.1623096875017893 1101 30-31 0.15327914156916478 1101 32-33 0.19667500906850677 1101 34-35 0.23967135651365368 1101 36-37 0.17222655699283962 1101 38-39 0.10459902410477895 1101 40-41 0.14104707218502455 1101 42-43 0.21831766368244132 1101 44-45 0.4283752044845599 1101 46-47 0.4266028364640704 1101 48-49 0.5647718139523121 1101 50-51 0.5228695010105753 1101 52-53 0.4393978753271952 1101 54-55 0.5735892859190983 1101 56-57 0.47482159920194533 1101 58-59 0.6604102389908775 1101 60-61 0.5985398826717869 1101 62-63 0.5341472243627408 1101 64-65 0.5978623145719553 1101 66-67 0.6072846907014551 1101 68-69 0.5953373680137162 1101 70-71 0.2838263878222662 1101 72-73 0.3629660508372474 1101 74-75 0.473922682654635 1101 76-77 0.496364441357688 1101 78-79 0.6656502413666061 1101 80-81 0.6327432361351875 1101 82-83 0.46689797769684205 1101 84-85 0.425480583422722 1101 86-87 0.3845970359622797 1101 88-89 0.3656290546771146 1101 90-91 0.3694986077506748 1101 92-93 0.14759208467157947 1101 94-95 0.2346532651871378 1101 96-97 0.2051314573184264 1101 98-99 -0.08550831587587737 1101 100-101 0.281601874915431 1102 1 0.339790549078554 1102 2 0.44166723715240863 1102 3 0.40981365900960753 1102 4 0.20772046565886626 1102 5 0.15023546890652995 1102 6 0.17311299940870128 1102 7 0.30904757696158214 1102 8 0.204662485082018 1102 9 0.41328711411109254 1102 10-11 0.4060688524592635 1102 12-13 0.38251879298844216 1102 14-15 0.5689764879662107 1102 16-17 0.588657828004628 1102 18-19 0.4815606209620995 1102 20-21 0.3813553051593104 1102 22-23 0.35168765935740254 1102 24-25 0.35402219063435325 1102 26-27 0.38105563999823033 1102 28-29 0.40194977717816727 1102 30-31 0.5860147946005654 1102 32-33 0.4378687493314075 1102 34-35 0.4548603617972944 1102 36-37 0.4654998558433405 1102 38-39 0.46805015576389053 1102 40-41 0.5936044846194264 1102 42-43 0.7067057734998485 1102 44-45 0.7978790974734764 1102 46-47 0.5885453364478579 1102 48-49 0.6493070417716424 1102 50-51 0.6975950084522538 1102 52-53 0.6291568723901975 1102 54-55 0.8456538814532593 1102 56-57 0.8048779898483929 1102 58-59 0.7759091171094141 1102 60-61 0.8128998726995036 1102 62-63 0.7685519830678658 1102 64-65 0.7717649467533221 1102 66-67 0.8248233448700191 1102 68-69 0.8229322923457971 1102 70-71 0.3802897735224242 1102 72-73 0.35986674324339774 1102 74-75 0.531472398083217 1102 76-77 0.6644176271581266 1102 78-79 0.786497171507822 1102 80-81 0.7497717841026876 1102 82-83 0.8730267127836022 1102 84-85 0.7416992775545523 1102 86-87 0.9140274250943499 1102 88-89 0.9041991168926451 1102 90-91 0.8074071491564858 1102 92-93 0.8403778678708846 1102 94-95 0.8691339976492252 1102 96-97 1.1037429841986324 1102 98-99 1.0884847943539562 1102 100-101 0.9417553191980979 1103 1 0.2465804755470522 1103 2 0.2748905767623455 1103 3 0.24263911174018915 1103 4 0.13185916706780176 1103 5 0.21645248600564315 1103 6 0.23915148058354418 1103 7 0.09755077043939053 1103 8 0.17490045103273388 1103 9 0.09991766146452363 1103 10-11 0.3071317856565159 1103 12-13 0.2864848444301842 1103 14-15 0.3474449122888217 1103 16-17 0.34355951032381427 1103 18-19 0.3263760260345805 1103 20-21 0.2563289603696859 1103 22-23 0.30285319104001474 1103 24-25 0.31915231062338023 1103 26-27 0.3316643209972554 1103 28-29 0.36182191872373437 1103 30-31 0.4771860499348648 1103 32-33 0.4344500728029388 1103 34-35 0.4588067695136786 1103 36-37 0.43440417276083565 1103 38-39 0.30465016344953 1103 40-41 0.26766901485392935 1103 42-43 0.487987928762621 1103 44-45 0.48572705456184195 1103 46-47 0.5470582897304013 1103 48-49 0.47231277998307775 1103 50-51 0.4249094295483218 1103 52-53 0.4562024632569859 1103 54-55 0.36081284645091216 1103 56-57 0.38397608746218737 1103 58-59 0.39407268895448766 1103 60-61 0.2512951927669178 1103 62-63 0.4276826276851864 1103 64-65 0.4471643194430044 1103 66-67 0.3431824708364175 1103 68-69 0.1863406147454043 1103 70-71 0.24254405689282876 1103 72-73 0.5107268543796231 1103 74-75 0.5070917423074022 1103 76-77 0.33017283769804706 1103 78-79 0.19244645155216134 1103 80-81 0.4599356576448308 1103 82-83 0.3928894310694311 1103 84-85 0.6156294046086437 1103 86-87 0.42948355215846235 1103 88-89 0.07742777439080939 1103 90-91 0.19051552642962832 1103 92-93 0.07136564174033566 1103 94-95 0.01571282686887443 1103 96-97 0.3793963648169374 1103 98-99 0.36978365752407427 1103 100-101 0.5699529441452 1104 1 0.20499266482883272 1104 2 0.34477026879871175 1104 3 0.3000349463780694 1104 4 0.1849196127767243 1104 5 0.1496094079310737 1104 6 0.19404526416868606 1104 7 0.16121922896859076 1104 8 0.19112445619542484 1104 9 0.24348570497016908 1104 10-11 0.23339248016838354 1104 12-13 0.14918083016207362 1104 14-15 0.32612599636442496 1104 16-17 0.2588188024337512 1104 18-19 0.2705009539929648 1104 20-21 0.3085903488384645 1104 22-23 0.2033710557645847 1104 24-25 0.20280279895933262 1104 26-27 0.2682236207932718 1104 28-29 0.29681686875073865 1104 30-31 0.3585939475376634 1104 32-33 0.545097522805996 1104 34-35 0.4183792370559658 1104 36-37 0.38548266675157805 1104 38-39 0.6188990680609052 1104 40-41 0.5396291533408188 1104 42-43 0.578517749878408 1104 44-45 0.6254680215193318 1104 46-47 0.7502562867340572 1104 48-49 0.7858213165615595 1104 50-51 0.6595890294499966 1104 52-53 0.7698213797461761 1104 54-55 0.5982998286199503 1104 56-57 0.871620167624279 1104 58-59 0.7043389537842586 1104 60-61 0.6803621645192379 1104 62-63 0.6573315311669745 1104 64-65 0.6663906090646634 1104 66-67 0.8279504115009004 1104 68-69 1.011986275832669 1104 70-71 0.8305065321432963 1104 72-73 0.741324975981204 1104 74-75 0.9446960292224844 1104 76-77 0.733714301622971 1104 78-79 0.7432020566784026 1104 80-81 0.7626295413699715 1104 82-83 0.8527581686056465 1104 84-85 0.5908332145449968 1104 86-87 0.7491877290865325 1104 88-89 0.8924692288616107 1104 90-91 0.7851625550490873 1104 92-93 0.9579751465925099 1104 94-95 0.9372090136665534 1104 96-97 0.9779824460452566 1104 98-99 0.8899825672799615 1104 100-101 0.5697146266739139 1105 1 0.19015027044590127 1105 2 0.26076322157110354 1105 3 0.16294430152189676 1105 4 0.18035793392791533 1105 5 0.13056127728296474 1105 6 0.18673864128949447 1105 7 0.23126055073269924 1105 8 0.1982921864553049 1105 9 0.36927595920899847 1105 10-11 0.24566644997351972 1105 12-13 0.22958223467134076 1105 14-15 0.3351433697985229 1105 16-17 0.28912655121426667 1105 18-19 0.3645784420400915 1105 20-21 0.28136231401657597 1105 22-23 0.19850473265147173 1105 24-25 0.2515121259858759 1105 26-27 0.36630552339953937 1105 28-29 0.2797788095113418 1105 30-31 0.3983636955153145 1105 32-33 0.36469851383144913 1105 34-35 0.19828162022585616 1105 36-37 0.4178123436278227 1105 38-39 0.3232818561197206 1105 40-41 0.2911140824027427 1105 42-43 0.23386732977662916 1105 44-45 0.29435067879162347 1105 46-47 0.2279580929914431 1105 48-49 0.03963959323322541 1105 50-51 0.012717464713333015 1105 52-53 0.10795860710588556 1105 54-55 0.06773740304183917 1105 56-57 0.09250867387287087 1105 58-59 0.12778044672021593 1105 60-61 -0.017645597497207177 1105 62-63 -0.00965284631703156 1105 64-65 -0.07885008047185948 1105 66-67 -0.040896240158595276 1105 68-69 -0.10424591584689225 1105 70-71 -0.17158423732907124 1105 72-73 -0.2915537380957538 1105 74-75 -0.041954672052163744 1105 76-77 0.022824128178999814 1105 78-79 0.10305867787588596 1105 80-81 0.22298077999450783 1105 82-83 0.16802469362119865 1105 84-85 0.08335672647633174 1105 86-87 0.32977896157462894 1105 88-89 0.4290910264369927 1105 90-91 0.3780102142850019 1105 92-93 0.43657031792042744 1105 94-95 0.4502401176281623 1105 96-97 0.4844322418077702 1105 98-99 0.5239574441700441 1105 100-101 0.31785318730598533 1106 1 0.14520628839393268 1106 2 0.26244691206199633 1106 3 0.20184838924663495 1106 4 0.20008858594891876 1106 5 0.19523118918375815 1106 6 0.1403610077635804 1106 7 0.22617004912231664 1106 8 0.24681722778021964 1106 9 0.2807175226824299 1106 10-11 0.1824342033930222 1106 12-13 0.24677176778781273 1106 14-15 0.22949043387060897 1106 16-17 0.38080244775997585 1106 18-19 0.3294812514728136 1106 20-21 0.21618901856136574 1106 22-23 0.24276116643398638 1106 24-25 0.23885481567376132 1106 26-27 0.23948037433827096 1106 28-29 0.3101596125592181 1106 30-31 0.12619410059032532 1106 32-33 0.12158857623258967 1106 34-35 0.13671393933891807 1106 36-37 0.26179921221142166 1106 38-39 0.3202429022130744 1106 40-41 0.20789051989688545 1106 42-43 0.35253173893018186 1106 44-45 0.21636483161386622 1106 46-47 0.24984536104719268 1106 48-49 0.3281679731338798 1106 50-51 0.32450218843911216 1106 52-53 0.2665468570665155 1106 54-55 0.24799404837766303 1106 56-57 0.19977340522023468 1106 58-59 0.27276199365187637 1106 60-61 0.10861596031433152 1106 62-63 0.34594100343147716 1106 64-65 0.42209884002777187 1106 66-67 0.03811632530547371 1106 68-69 0.25948706987161074 1106 70-71 0.4149755398221764 1106 72-73 0.2166036962914326 1106 74-75 0.06085339124572542 1106 76-77 0.06293296008123761 1106 78-79 -0.008563792013770666 1106 80-81 0.20434130391006633 1106 82-83 0.1646475141321453 1106 84-85 0.3161426970916672 1106 86-87 0.29048305161322574 1106 88-89 0.2738825531601172 1106 90-91 0.3341908175377739 1106 92-93 0.2863536528553645 1106 94-95 0.4968629499445356 1106 96-97 0.6805039139926912 1106 98-99 0.6770344469790288 1106 100-101 0.3481492273632263 1107 1 0.07544583036396446 1107 2 0.20536686710022423 1107 3 0.08274889302644084 1107 4 0.1272705223407442 1107 5 0.048346978799791884 1107 6 0.07381879967992688 1107 7 0.02187890930039771 1107 8 0.06663206940795163 1107 9 -0.053047148939732836 1107 10-11 -0.016558236995443565 1107 12-13 0.05858714940445253 1107 14-15 0.10546505439199905 1107 16-17 0.11285486958457369 1107 18-19 0.13631759030140245 1107 20-21 0.06593349142750071 1107 22-23 0.07906397244111218 1107 24-25 0.1342484025360804 1107 26-27 0.07300839279115934 1107 28-29 -0.011776480826213742 1107 30-31 0.1683188310330408 1107 32-33 0.11871819478731993 1107 34-35 0.12120767758932516 1107 36-37 0.22535287547424332 1107 38-39 0.18205995089392246 1107 40-41 0.07014508549609388 1107 42-43 0.09783662165629181 1107 44-45 -0.0048318546821874975 1107 46-47 0.010978260570787768 1107 48-49 0.04156769525875603 1107 50-51 -0.05026911483437857 1107 52-53 -0.0629718137027595 1107 54-55 -0.0172743361345411 1107 56-57 -0.10525632564565512 1107 58-59 0.018089259337841668 1107 60-61 -0.13013153122035703 1107 62-63 -0.41117555373355685 1107 64-65 -0.3199324182419758 1107 66-67 -0.2324269767453444 1107 68-69 -0.13864178463146004 1107 70-71 -0.37923727596116663 1107 72-73 -0.3353946007383719 1107 74-75 -0.1532861343334524 1107 76-77 -0.2702322678973701 1107 78-79 -0.011381247085228807 1107 80-81 0.011062347179827015 1107 82-83 0.1490662927826243 1107 84-85 0.11900722632556437 1107 86-87 0.10035356385892058 1107 88-89 -0.06797833814333032 1107 90-91 -0.17323484597126182 1107 92-93 -0.20750478937923234 1107 94-95 -0.005567699601964904 1107 96-97 0.22382100335921962 1107 98-99 0.6922953122156557 1107 100-101 0.4081378913266853 1108 1 -0.04299757040915253 1108 2 0.07148468040461609 1108 3 -0.08478504647195706 1108 4 -0.013867981581569211 1108 5 -0.03188584822474638 1108 6 -0.12825244632024635 1108 7 -0.06125938854668789 1108 8 0.008119761636017131 1108 9 -0.0967756956297805 1108 10-11 -0.0799949830580502 1108 12-13 -0.014542848454063062 1108 14-15 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-1.3278300345085583 2316 46-47 -1.274634315790692 2316 48-49 -1.4902281316912607 2316 50-51 -1.5916446914522453 2316 52-53 -1.5799424430081928 2316 54-55 -1.3446873375563584 2316 56-57 -1.425882346712207 2316 58-59 -1.6146975981186173 2316 60-61 -1.2365004437566753 2316 62-63 -1.3209688930423127 2316 64-65 -1.238153860692485 2316 66-67 -1.2402893216120958 2316 68-69 -1.4574147884264903 2316 70-71 -0.9307276358215724 2316 72-73 -0.9248172518350337 2316 74-75 -1.3289381866374868 2316 76-77 -1.3841901814147555 2316 78-79 -1.5016235060499525 2316 80-81 -1.4068931676919867 2316 82-83 -1.492155024650149 2316 84-85 -1.3269755720801406 2316 86-87 -1.5038709764889653 2316 88-89 -1.5925252547607336 2316 90-91 -1.9885518507155808 2316 92-93 -1.9785210141399467 2316 94-95 -1.7845532759443685 2316 96-97 -1.600280087261691 2316 98-99 -1.2338276755813382 2316 100-101 -1.5508276821763296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 32.0 5 49.0 6 113.0 7 268.0 8 659.0 9 1337.0 10 2330.0 11 3886.0 12 5609.0 13 8063.0 14 10519.0 15 15020.0 16 19878.0 17 26424.0 18 22545.0 19 14000.0 20 11920.0 21 12287.0 22 12703.0 23 14538.0 24 16763.0 25 20208.0 26 24248.0 27 29965.0 28 37277.0 29 46174.0 30 56961.0 31 71614.0 32 89220.0 33 115459.0 34 153523.0 35 210850.0 36 297286.0 37 369873.0 38 193303.0 39 7403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64586758456499 19.781308920789648 15.165977133010625 21.406846361634734 2 15.647704245539465 21.20969650935028 39.7285310345007 23.414068210609557 3 19.66898500631305 25.9629936182798 30.05032231146561 24.31769906394154 4 12.970339315895623 17.568091290214007 37.18975461281199 32.271814781078376 5 13.116251679042943 38.49604573031475 33.586789482610264 14.800913108032043 6 30.666690249318922 37.180184121557474 17.24210939194732 14.911016237176284 7 28.20625798843163 34.04641600656709 20.252550185687955 17.494775819313315 8 27.002147977940698 34.101815923128505 19.277490762238248 19.61854533669255 9 27.020985612674703 17.025079630466774 20.360533668484436 35.59340108837408 10-11 25.01093732199216 27.23068047569678 26.581281859339533 21.177100342971528 12-13 26.672994100348696 24.78743100123445 27.409579169304543 21.129995729112316 14-15 23.138953943857015 25.929404579707914 26.658867006605607 24.272774469829464 16-17 21.367539375419273 29.351819120830015 27.358148736873016 21.922492766877696 18-19 21.28451463299489 27.182138941596772 30.737001455301183 20.79634497010715 20-21 22.38143828189193 25.95361651313101 30.866063864624703 20.798881340352356 22-23 21.942344902137297 26.038093221672565 30.801657692656832 21.217904183533307 24-25 21.968941485413097 26.34051168725911 30.989170824431266 20.70137600289652 26-27 21.54971640934645 26.422830205070664 31.039742227202332 20.987711158380556 28-29 21.127628793794155 26.730138681601424 31.139088922378566 21.003143602225855 30-31 21.32743728819379 26.337931197720653 31.038588819519063 21.2960426945665 32-33 21.18282839501902 26.608579663791797 30.925931658075523 21.282660283113664 34-35 21.923878647390442 26.445209616193942 30.576981000354266 21.053930736061353 36-37 22.0261396427492 26.0411404932968 30.687679440833882 21.24504042312012 38-39 21.558426221593777 26.40448298357821 30.81504961497283 21.222041179855186 40-41 21.684306408440026 26.543339767410828 30.689795358880804 21.082558465268335 42-43 21.65578124065245 26.743840045362443 30.068876004983625 21.53150270900148 44-45 21.682374215619664 26.764211967633223 29.928421230058195 21.62499258668892 46-47 22.254029902456253 27.113452151752288 29.174922762478822 21.457595183312637 48-49 22.38813656928736 27.33102461299802 28.67534179517312 21.6054970225415 50-51 22.23083173628148 27.55918455676869 28.626192066553884 21.583791640395944 52-53 22.47251156617161 27.710276472272792 28.32648869835409 21.49072326320151 54-55 21.95404131532136 27.66640300866638 28.4016889024605 21.977866773551767 56-57 22.263679165401758 27.947589960742935 28.1471139646252 21.641616909230105 58-59 22.45578541653553 27.386354591673502 28.10240793061131 22.055452061179658 60-61 22.05900560694766 27.63741911855745 28.757143942176338 21.546431332318555 62-63 22.091326839949012 27.628087064792435 28.74039147059887 21.540194624659687 64-65 21.922281781993203 27.776821145198443 28.471501135126925 21.82939593768143 66-67 21.950071879908663 27.88248577813025 28.12698411046955 22.04045823149154 68-69 21.95816313578279 27.744637086453768 28.377974858969264 21.919224918794182 70-71 22.107381317842947 27.857542134126373 28.13451290418991 21.90056364384077 72-73 22.17541964948532 27.617012067572578 28.156143370898512 22.05142491204359 74-75 22.00467907935446 28.407172507365363 27.747580472725396 21.840567940554777 76-77 22.22213263035508 27.974364009733144 27.903615335295395 21.899888024616377 78-79 22.23442482143944 27.956114343268073 27.766979835883998 22.042480999408482 80-81 22.219817647078717 28.312615115061092 27.619343153884106 21.848224083976085 82-83 22.267183731245723 28.320600501024444 27.615675598767393 21.796540168962444 84-85 22.00728303702644 28.263515886124512 27.511152150446737 22.218048926402307 86-87 22.102579975602207 28.06622778383243 27.575644863848346 22.255547376717015 88-89 22.116072150835585 28.11390232996936 27.322705841011068 22.447319678183987 90-91 22.021057012609496 28.040865431512685 27.627086679716207 22.310990876161615 92-93 22.105023310884274 28.008513856996753 27.67984131327519 22.20662151884379 94-95 21.82245651252805 27.7692709253181 27.728096429991783 22.680176132162067 96-97 21.95229608451472 27.658641971455268 27.76216293262918 22.62689901140083 98-99 21.70911439414807 28.00080424261388 27.372262109222493 22.917819254015555 100-101 21.53320374732966 28.197623077441293 27.447392426319745 22.8217807489093 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 591.0 1 420.5 2 319.5 3 406.5 4 378.0 5 387.5 6 612.0 7 1165.0 8 1844.5 9 2305.0 10 2393.5 11 2300.5 12 2349.0 13 2561.0 14 2866.0 15 3148.0 16 3472.0 17 3823.0 18 4091.5 19 4375.5 20 4755.5 21 5325.0 22 6127.0 23 7021.5 24 8054.5 25 9676.0 26 12033.5 27 14850.5 28 17617.0 29 20707.0 30 24805.0 31 29398.0 32 34575.0 33 39341.5 34 44256.0 35 48206.0 36 52966.5 37 57438.0 38 58342.0 39 58300.5 40 58627.0 41 60517.5 42 62079.0 43 63548.0 44 65983.5 45 68459.5 46 68196.5 47 70120.5 48 80705.0 49 79208.5 50 67028.0 51 60615.0 52 57128.5 53 56393.0 54 55125.5 55 51924.5 56 47218.5 57 42577.0 58 39469.5 59 35263.0 60 29660.0 61 24863.0 62 20974.5 63 16220.5 64 11319.5 65 8592.0 66 6638.0 67 5161.0 68 3750.5 69 2470.5 70 1921.5 71 1548.5 72 1279.0 73 1144.5 74 932.5 75 757.5 76 572.5 77 367.5 78 231.5 79 141.5 80 95.5 81 67.0 82 45.0 83 33.0 84 18.0 85 5.0 86 4.5 87 3.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012745036328555592 2 0.009467741272641297 3 0.005618220095853077 4 4.1616445154467243E-4 5 0.004733870636320648 6 0.0 7 3.1212333865850427E-4 8 0.0022889044834956984 9 0.0 10-11 0.0 12-13 0.0 14-15 5.722261208739246E-4 16-17 0.004343716462997518 18-19 0.0017947091972863997 20-21 8.583391813108868E-4 22-23 3.381336168800463E-4 24-25 5.202055644308405E-4 26-27 2.0808222577233621E-4 28-29 0.0 30-31 5.982363990954666E-4 32-33 0.004447757575883686 34-35 9.363700159755129E-4 36-37 0.0013525344675201852 38-39 0.005982363990954666 40-41 2.6010278221542027E-5 42-43 0.0011444522417478492 44-45 0.005150035087865321 46-47 0.0 48-49 0.002080822257723362 50-51 0.0013005139110771012 52-53 4.421747297662144E-4 54-55 1.820719475507942E-4 56-57 0.01261498493744788 58-59 0.0022889044834956984 60-61 0.0030692128301419586 62-63 7.803083466462607E-5 64-65 0.0033813361688004627 66-67 7.022775119816347E-4 68-69 0.002132842814166446 70-71 0.004811901470985275 72-73 0.003017192273698875 74-75 0.007490960127804103 76-77 0.001014400850640139 78-79 0.007282877902031767 80-81 0.0028871408825911645 82-83 0.0028871408825911645 84-85 0.00218486337060953 86-87 6.242466773170085E-4 88-89 0.002002791423058736 90-91 0.008037175970456487 92-93 0.002028801701280278 94-95 5.462158426523825E-4 96-97 5.2020556443084054E-5 98-99 5.982363990954666E-4 100-101 0.002158853092387988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1922317.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.997929303976925 #Duplication Level Percentage of deduplicated Percentage of total 1 75.33113443706466 37.6640073397274 2 13.773054165971226 13.772483769801482 3 4.573169219905838 6.85946974055926 4 1.995300906375042 3.9904365462840166 5 1.0968155271673234 2.741925259340801 6 0.6565026175052282 1.9694262874741322 7 0.44318448168765534 1.5510814468827332 8 0.2973891586441144 1.1895073703726087 9 0.2380218197661823 1.0710538305726167 >10 1.3824175734380377 13.130414193258572 >50 0.13032459878667166 4.527537619552666 >100 0.07558717532190844 7.00056801872211 >500 0.0050105776702169015 1.6953840037534653 >1k 0.0017745795915351523 1.5063469128288547 >5k 2.0877406959237088E-4 0.7771292887541281 >10k+ 1.0438703479618544E-4 0.5532283721150946 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10600 0.5514178982966909 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9376 0.48774473721035605 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5514 0.28684134822716545 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4899 0.25484870601466875 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2872 0.1494030381045374 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1951 0.10149210562045699 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1933 0.10055573560448147 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 7.803083466462607E-5 16-17 0.0 0.0 0.0 0.0 1.040411128861681E-4 18-19 0.0 0.0 0.0 2.6010278221542023E-5 1.040411128861681E-4 20-21 0.0 0.0 0.0 5.202055644308405E-5 1.040411128861681E-4 22-23 0.0 0.0 0.0 1.040411128861681E-4 1.300513911077101E-4 24-25 0.0 0.0 0.0 2.080822257723362E-4 1.5606166932925213E-4 26-27 0.0 0.0 0.0 3.121233386585043E-4 1.5606166932925213E-4 28-29 0.0 0.0 0.0 9.623802941970548E-4 1.5606166932925213E-4 30-31 0.0 0.0 0.0 0.0019247605883941097 1.5606166932925213E-4 32-33 0.0 0.0 0.0 0.0029651717172557907 2.6010278221542027E-4 34-35 0.0 0.0 0.0 0.004395737019440602 3.381336168800463E-4 36-37 0.0 0.0 0.0 0.006372518164277796 3.641438951015883E-4 38-39 0.0 0.0 0.0 0.007907124579348775 3.641438951015883E-4 40-41 0.0 0.0 0.0 0.009987946837072138 3.641438951015883E-4 42-43 0.0 0.0 0.0 0.01360337550986648 3.641438951015883E-4 44-45 0.0 0.0 0.0 0.01976781144837194 3.9015417332313035E-4 46-47 0.0 0.0 0.0 0.035530040050626405 4.421747297662144E-4 48-49 0.0 0.0 0.0 0.06708050753335687 4.6818500798775643E-4 50-51 0.0 0.0 0.0 0.08983950097720615 4.6818500798775643E-4 52-53 0.0 0.0 0.0 0.11046565160688898 5.202055644308405E-4 54-55 0.0 0.0 0.0 0.13902493709414213 5.722261208739246E-4 56-57 0.0 0.0 0.0 0.16347459862239164 5.722261208739246E-4 58-59 0.0 0.0 0.0 0.1954152202784452 5.722261208739246E-4 60-61 0.0 0.0 0.0 0.2502188764912343 5.722261208739246E-4 62-63 0.0 5.202055644308405E-5 0.0 0.29227749637546774 6.242466773170085E-4 64-65 0.0 5.202055644308405E-5 0.0 0.3397202438515604 6.242466773170085E-4 66-67 0.0 5.202055644308405E-5 0.0 0.42958575510698804 6.502569555385505E-4 68-69 0.0 5.202055644308405E-5 0.0 0.5343551557833593 7.022775119816347E-4 70-71 0.0 5.202055644308405E-5 0.0 0.6167297069109829 7.542980684247186E-4 72-73 0.0 5.202055644308405E-5 0.0 0.7113811093591744 7.803083466462607E-4 74-75 0.0 5.202055644308405E-5 0.0 0.7956023902405274 7.803083466462607E-4 76-77 0.0 5.202055644308405E-5 0.0 0.860315962455724 8.063186248678028E-4 78-79 0.0 5.202055644308405E-5 0.0 0.9516900698480011 8.323289030893448E-4 80-81 0.0 5.202055644308405E-5 0.0 1.084940725176961 8.843494595324288E-4 82-83 0.0 5.202055644308405E-5 0.0 1.234239722168612 9.363700159755129E-4 84-85 0.0 5.202055644308405E-5 0.0 1.4123060868732888 9.363700159755129E-4 86-87 0.0 5.202055644308405E-5 0.0 1.6902259096704655 9.363700159755129E-4 88-89 0.0 5.202055644308405E-5 0.0 2.0619387957345223 9.363700159755129E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11725 0.0 24.919733 1 GTATCAA 19395 0.0 24.667255 1 TCGCGTA 135 1.3442204E-5 24.627548 9 GGTATCA 14345 0.0 23.481554 1 TACATGG 12215 0.0 23.337278 2 GAGTACT 11100 0.0 22.851498 12-13 ACATGGG 12075 0.0 22.504986 3 TAAGGTG 1120 0.0 22.479286 5 GTAAGGT 910 0.0 22.44666 4 TACCTGG 1685 0.0 21.993135 2 GTACTTT 11575 0.0 21.893515 14-15 AGTACTT 11480 0.0 21.660635 12-13 CGCGTAA 80 4.9934468E-5 20.781113 10-11 ATCAACG 23225 0.0 20.496601 3 CATGGGA 6585 0.0 20.48742 4 TCAACGC 23270 0.0 20.434422 4 TATCAAC 23305 0.0 20.427303 2 CAACGCA 23335 0.0 20.418217 5 AACGCAG 23705 0.0 20.097425 6 GTACCTG 1915 0.0 19.59925 1 >>END_MODULE