FastQCFastQC Report
Thu 26 May 2016
SRR936682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921026
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94280.49077940642136025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74480.38770948441093456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43220.2249839408732625No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37680.19614518491681007No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27110.14112250432841617No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.10510008714093406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA171100.026.6067581
GTACATG118900.025.4585721
GAGTACT88350.024.2271612-13
TACATGG121500.024.1694092
GGTATCA124000.023.874971
TATCACG1201.7071229E-423.7587642
ACATGGG119850.023.471323
GTACTTT93850.022.62956614-15
TATCAAC200200.022.5720142
ATCAACG200750.022.510173
TCAACGC200400.022.502654
CAACGCA201900.022.3125115
AACGCAG204250.022.1029036
AGTACTT92950.021.2135312-13
CATGGGG46350.021.1194534
CGTATAG1604.975364E-520.791
TACCTGG16700.020.2020632
GTACCTG18350.020.1992381
ACGCAGA224000.020.1535747
CGCAGAG224650.020.0318038
Produced by FastQC (version 0.11.5)