##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936682_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1921026 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50991969916076 31.0 31.0 34.0 30.0 34.0 2 31.569543046267984 33.0 31.0 34.0 30.0 34.0 3 31.60188305624182 33.0 31.0 34.0 28.0 34.0 4 35.2510647955832 37.0 35.0 37.0 33.0 37.0 5 35.18756851807316 37.0 35.0 37.0 33.0 37.0 6 35.30856271596532 37.0 35.0 37.0 33.0 37.0 7 35.168105481133516 37.0 35.0 37.0 32.0 37.0 8 35.15393909296387 37.0 35.0 37.0 32.0 37.0 9 36.67155259741409 39.0 37.0 39.0 32.0 39.0 10-11 36.55431264334788 39.0 37.0 39.0 32.0 39.0 12-13 36.47731082244592 39.0 37.0 39.0 32.0 39.0 14-15 37.55970741676583 40.0 37.0 41.0 32.0 41.0 16-17 37.50538670481295 40.0 37.0 41.0 32.0 41.0 18-19 37.50710557795678 40.0 37.0 41.0 32.0 41.0 20-21 37.437433434008696 40.0 37.0 41.0 32.0 41.0 22-23 37.343028933496996 39.5 37.0 41.0 32.0 41.0 24-25 37.200554807691304 39.0 36.0 41.0 31.0 41.0 26-27 37.00705534438368 39.0 36.0 41.0 31.0 41.0 28-29 37.11238890051462 39.0 36.0 41.0 31.0 41.0 30-31 37.02685205718194 39.0 36.0 41.0 31.0 41.0 32-33 36.8614086951452 39.0 36.0 41.0 30.5 41.0 34-35 36.689873796606605 39.0 35.0 41.0 30.0 41.0 36-37 36.46180738834352 39.0 35.0 41.0 30.0 41.0 38-39 36.27967086338238 39.0 35.0 40.0 30.0 41.0 40-41 36.12844021892468 39.0 35.0 40.0 30.0 41.0 42-43 35.853324733762065 38.0 35.0 40.0 29.0 41.0 44-45 35.54767530475902 38.0 35.0 40.0 27.5 41.0 46-47 35.14747249646803 38.0 34.5 40.0 26.0 41.0 48-49 34.84815249767572 38.0 34.0 40.0 24.5 41.0 50-51 34.13432093058605 37.5 33.5 39.5 23.5 40.5 52-53 34.309912255221946 38.0 33.5 39.5 24.0 40.5 54-55 34.66343870410916 38.0 34.0 40.0 24.0 41.0 56-57 34.598214183462375 38.0 34.0 40.0 24.0 41.0 58-59 34.37546654756365 38.0 34.0 40.0 23.5 41.0 60-61 34.07341129167435 38.0 34.0 40.0 23.0 41.0 62-63 33.75903865954963 37.0 33.0 40.0 22.0 41.0 64-65 33.452844729847484 37.0 33.0 40.0 22.0 41.0 66-67 33.0885906801886 36.0 33.0 39.0 20.5 41.0 68-69 32.71419855847864 36.0 32.0 39.0 20.0 41.0 70-71 32.31840381129667 35.0 32.0 38.5 18.5 40.0 72-73 31.914872313024397 35.0 31.5 38.0 17.5 40.0 74-75 31.4938358460531 35.0 31.0 37.0 14.5 39.0 76-77 31.084194071293155 35.0 31.0 37.0 11.5 39.0 78-79 30.638370849743836 35.0 30.5 36.0 7.5 39.0 80-81 30.22721035529972 34.0 30.0 36.0 7.0 37.5 82-83 29.82653826653049 34.0 30.0 35.0 2.0 37.0 84-85 29.449148007366894 34.0 29.0 35.0 2.0 36.5 86-87 29.120865620767233 34.0 29.0 35.0 2.0 36.0 88-89 28.748463321162752 34.0 29.0 35.0 2.0 36.0 90-91 28.400353248732706 34.0 29.0 35.0 2.0 35.5 92-93 28.05995572157795 34.0 28.5 35.0 2.0 35.0 94-95 27.66272554353767 34.0 27.0 35.0 2.0 35.0 96-97 27.126072473771828 33.5 26.5 35.0 2.0 35.0 98-99 26.509834067836668 33.0 25.0 35.0 2.0 35.0 100-101 24.46295469191984 31.0 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16124978276408442 1101 2 0.2540219371629213 1101 3 0.18730765166373686 1101 4 0.27829586798784334 1101 5 0.23620157079852788 1101 6 0.09073736970855606 1101 7 0.18922148374174697 1101 8 0.04923726449330701 1101 9 0.368852962735275 1101 10-11 0.12538924315946076 1101 12-13 0.2753795934178527 1101 14-15 0.3955918780752228 1101 16-17 0.43663236554148455 1101 18-19 0.43030833368754884 1101 20-21 0.4880242170664033 1101 22-23 0.2714929485495361 1101 24-25 0.18962146041190664 1101 26-27 0.24181972124083728 1101 28-29 0.3699376656275035 1101 30-31 0.1607711910723566 1101 32-33 0.17437300379598497 1101 34-35 -0.024669412883589814 1101 36-37 0.21939603922075435 1101 38-39 0.1215533195808618 1101 40-41 0.16084754182674033 1101 42-43 -0.07758982024004979 1101 44-45 2.0715355551459425E-4 1101 46-47 0.16027335305324186 1101 48-49 0.4045633179617525 1101 50-51 0.48817522170639904 1101 52-53 0.4221536728953623 1101 54-55 0.3033347680481242 1101 56-57 0.26982521243218827 1101 58-59 0.29939927049992576 1101 60-61 0.28893363452136356 1101 62-63 0.4252221404528953 1101 64-65 0.4705673522425684 1101 66-67 0.462063257696947 1101 68-69 0.37342184220171504 1101 70-71 0.18154136626048256 1101 72-73 0.19352030084290917 1101 74-75 0.3045523895243605 1101 76-77 0.30492277853773686 1101 78-79 0.3396999043829858 1101 80-81 0.20444131527424858 1101 82-83 0.22232258047663933 1101 84-85 0.13019199183868224 1101 86-87 0.1735776400017386 1101 88-89 0.062422923238720784 1101 90-91 0.02767631264615389 1101 92-93 -0.10479490144922465 1101 94-95 -0.047513524145447406 1101 96-97 -0.27433973526618516 1101 98-99 -0.38305117410500245 1101 100-101 -0.09111878977253696 1102 1 0.25910431932293676 1102 2 0.2471548275776918 1102 3 0.2722840717259274 1102 4 0.36412516339532885 1102 5 0.3386694658724849 1102 6 0.22077676248024147 1102 7 0.11742676421057752 1102 8 0.16637788586592706 1102 9 0.3720493088830352 1102 10-11 0.4782544761607497 1102 12-13 0.4583875152183978 1102 14-15 0.34110112576989593 1102 16-17 0.17870384140096007 1102 18-19 0.36336590255820767 1102 20-21 0.14225254453352676 1102 22-23 0.09854374906685592 1102 24-25 0.10164502335245373 1102 26-27 0.20549076007699796 1102 28-29 0.1771448065436374 1102 30-31 0.06509886646851726 1102 32-33 0.12712889490764923 1102 34-35 0.14727899203080597 1102 36-37 -0.045763959892802575 1102 38-39 -0.1191641843946698 1102 40-41 -0.1627542127211541 1102 42-43 -0.04426001545255076 1102 44-45 0.023662624060207804 1102 46-47 0.16394798928490673 1102 48-49 0.38974525430291607 1102 50-51 0.2890325937960725 1102 52-53 0.3794096432917229 1102 54-55 0.287189304176664 1102 56-57 0.36398843822878035 1102 58-59 0.44042147731703096 1102 60-61 -0.11071753429867925 1102 62-63 0.029446670430154143 1102 64-65 0.13004310069770497 1102 66-67 0.15636833533894645 1102 68-69 0.22981680438496 1102 70-71 0.27708050977483367 1102 72-73 0.0014428329101825454 1102 74-75 -0.038425061992022336 1102 76-77 0.09806446880155661 1102 78-79 0.13517290917858205 1102 80-81 0.22929185231246763 1102 82-83 0.16293177530766556 1102 84-85 0.024562175454310164 1102 86-87 -0.011598831396494091 1102 88-89 -0.21344976252469294 1102 90-91 -0.19397467243132382 1102 92-93 -0.21808209259119948 1102 94-95 0.01926743298121636 1102 96-97 -0.23017675689810702 1102 98-99 -0.3138023864509556 1102 100-101 -0.18030138707057475 1103 1 0.2722582863949974 1103 2 0.3004570531092554 1103 3 0.3394892784218264 1103 4 0.3663366854162362 1103 5 0.4120221036927276 1103 6 0.307864708207056 1103 7 0.4026080884983827 1103 8 0.23373333662569706 1103 9 0.46278811917508733 1103 10-11 0.4755347497329012 1103 12-13 0.4814483243521721 1103 14-15 0.5565089744227123 1103 16-17 0.654957447203337 1103 18-19 0.544798736732659 1103 20-21 0.43038050570393693 1103 22-23 0.4299519286537361 1103 24-25 0.4678690105563348 1103 26-27 0.5447205662047949 1103 28-29 0.596001429361543 1103 30-31 0.4716997875683262 1103 32-33 0.5590106411363749 1103 34-35 0.49906829734505465 1103 36-37 0.699685972541495 1103 38-39 0.6327009048196359 1103 40-41 0.48323017822228564 1103 42-43 0.6413666357705239 1103 44-45 0.7217585397823356 1103 46-47 0.7820355213441417 1103 48-49 0.815051561354565 1103 50-51 0.339493419476554 1103 52-53 0.6922882732247047 1103 54-55 0.9561054399699387 1103 56-57 0.9348575262296634 1103 58-59 0.992649601196689 1103 60-61 1.2627380240146593 1103 62-63 1.043419478681436 1103 64-65 1.0691119625603136 1103 66-67 1.0822008275482133 1103 68-69 1.0522073617330747 1103 70-71 1.1242870402069727 1103 72-73 1.1072710229766436 1103 74-75 0.9718175678306586 1103 76-77 0.9161928160616917 1103 78-79 0.9559471845708742 1103 80-81 1.011687704605567 1103 82-83 0.9957573454794719 1103 84-85 0.8514436183268188 1103 86-87 1.0237873962309827 1103 88-89 0.8920334839384481 1103 90-91 0.9335054301582346 1103 92-93 0.9440169118488342 1103 94-95 1.1012942937958634 1103 96-97 1.293821698742942 1103 98-99 1.253902420366284 1103 100-101 1.2709658146061606 1104 1 0.18791531142506912 1104 2 0.23444250473878725 1104 3 0.25975206552303476 1104 4 0.20414824392474173 1104 5 0.2750176933599491 1104 6 0.28071401229091464 1104 7 0.3325445109738965 1104 8 0.2832410976658508 1104 9 0.5284379272969701 1104 10-11 0.46352260699850234 1104 12-13 0.33066924193050085 1104 14-15 0.34454517351773717 1104 16-17 0.19562655769076542 1104 18-19 0.358560464208459 1104 20-21 0.38771758113781374 1104 22-23 0.3833628914809353 1104 24-25 0.3521228624378878 1104 26-27 0.32037048888077635 1104 28-29 0.2272638155234077 1104 30-31 0.20979395103702103 1104 32-33 0.28074063712698205 1104 34-35 0.42269462187682194 1104 36-37 0.46515421241777233 1104 38-39 0.36135260308413564 1104 40-41 0.2341317189749077 1104 42-43 0.20197136685478512 1104 44-45 0.24200803860815512 1104 46-47 0.3404599442778746 1104 48-49 0.32622871926739094 1104 50-51 0.24297070912659535 1104 52-53 0.3889033141777958 1104 54-55 0.3838480369113455 1104 56-57 0.35523406555035564 1104 58-59 0.37885236195045024 1104 60-61 0.43625494938266485 1104 62-63 0.38516631412448277 1104 64-65 0.30976469181170074 1104 66-67 0.4196125922540901 1104 68-69 0.3499978422875145 1104 70-71 0.32115891144817255 1104 72-73 0.036703751082491465 1104 74-75 -0.08872438760780099 1104 76-77 0.009231478069828825 1104 78-79 0.3007937393563722 1104 80-81 0.3283487963061198 1104 82-83 0.3396961735910473 1104 84-85 0.42440529998097887 1104 86-87 0.40995441610612104 1104 88-89 0.22739736503129393 1104 90-91 0.18274284352669667 1104 92-93 -0.06628253256378969 1104 94-95 0.12316587865181106 1104 96-97 0.1413386241109471 1104 98-99 0.2173833757474668 1104 100-101 0.2737809815687591 1105 1 0.24524831923477564 1105 2 0.25219387398843907 1105 3 0.25586676301017874 1105 4 0.25024496449672284 1105 5 0.4812114797369773 1105 6 0.38786977820763724 1105 7 0.5047271182846345 1105 8 0.13123221315966305 1105 9 0.46801021976850876 1105 10-11 0.48624743049570185 1105 12-13 0.3387366806349448 1105 14-15 0.43962541159503843 1105 16-17 0.437674276148428 1105 18-19 0.44585698412564767 1105 20-21 0.21697980281749096 1105 22-23 0.07865251055153522 1105 24-25 0.024671683829367907 1105 26-27 0.06127331149487958 1105 28-29 0.2924973675997151 1105 30-31 0.3001596596660363 1105 32-33 0.2405674769951034 1105 34-35 0.21414094814876705 1105 36-37 0.22923485771750052 1105 38-39 0.09988647836345166 1105 40-41 0.13659884792327404 1105 42-43 0.23240218338820284 1105 44-45 0.23162648931278085 1105 46-47 0.20396246724493494 1105 48-49 0.3298534280365999 1105 50-51 0.13145804469980504 1105 52-53 0.4533815497125815 1105 54-55 0.5487991082305825 1105 56-57 0.4785397293753775 1105 58-59 0.47752440675063923 1105 60-61 0.5060165859781236 1105 62-63 0.23950370982737468 1105 64-65 0.28255541476979573 1105 66-67 0.16226685278298447 1105 68-69 0.35602726148533037 1105 70-71 0.4045329352349185 1105 72-73 0.4527177015501316 1105 74-75 0.36306052456280824 1105 76-77 0.4836282023611709 1105 78-79 0.3212175653970526 1105 80-81 0.5972398161455779 1105 82-83 0.515138229272754 1105 84-85 0.4996906656276181 1105 86-87 0.4340005667097522 1105 88-89 0.43357660775016527 1105 90-91 0.31698056987131906 1105 92-93 0.2618331243279499 1105 94-95 0.2589187680713749 1105 96-97 0.5323539335761467 1105 98-99 0.2942361185860243 1105 100-101 0.6152908707794644 1106 1 0.1667559787253019 1106 2 0.1364148389222315 1106 3 0.11505396666593981 1106 4 0.2219669879120474 1106 5 0.39160642917704536 1106 6 0.26176681597670637 1106 7 0.4128641369447479 1106 8 0.5077824297262055 1106 9 0.4945028500449453 1106 10-11 0.44846416737701844 1106 12-13 0.5396317032509614 1106 14-15 0.37020542031741854 1106 16-17 0.3884200100395603 1106 18-19 0.4708065563522652 1106 20-21 0.467833073537669 1106 22-23 0.4461196095191866 1106 24-25 0.3998601914364812 1106 26-27 0.39589309758616054 1106 28-29 0.43559574984836047 1106 30-31 0.36579591597470795 1106 32-33 0.2844770843853368 1106 34-35 0.17332222920266105 1106 36-37 0.25375540841179145 1106 38-39 0.25940637561364355 1106 40-41 0.2811876700646039 1106 42-43 0.3433134270807585 1106 44-45 0.11211242166492497 1106 46-47 0.19203099419595304 1106 48-49 0.03932166159856365 1106 50-51 0.11925883088849787 1106 52-53 0.18378593615794614 1106 54-55 0.3231073507792459 1106 56-57 0.32443191550562034 1106 58-59 0.26250972995583766 1106 60-61 0.08557707507736012 1106 62-63 0.09724856413871663 1106 64-65 0.09186317518928888 1106 66-67 0.2394526722141066 1106 68-69 0.1901211107409182 1106 70-71 0.10463122105772982 1106 72-73 0.2419980124230996 1106 74-75 0.1485475557778564 1106 76-77 0.06689264261900618 1106 78-79 0.22682609805530873 1106 80-81 0.18419168790802942 1106 82-83 0.19214044804583708 1106 84-85 0.19686631874131777 1106 86-87 0.48839097679364585 1106 88-89 0.3907513015002557 1106 90-91 0.4634571601755795 1106 92-93 0.449632221840897 1106 94-95 0.3866171617736427 1106 96-97 0.4369625988811059 1106 98-99 0.6792759688861452 1106 100-101 0.7502614609006564 1107 1 0.38628137357348535 1107 2 0.4565648799879334 1107 3 0.4689939121161011 1107 4 0.41405833858828345 1107 5 0.42319759876637875 1107 6 0.31503122854653753 1107 7 0.5560214140275974 1107 8 0.5280575326390959 1107 9 0.815227230806471 1107 10-11 0.7649783812485254 1107 12-13 0.6721359320652454 1107 14-15 0.7720231681599117 1107 16-17 0.8288287443125597 1107 18-19 0.8506866826241222 1107 20-21 0.8722208464836925 1107 22-23 0.832549528887867 1107 24-25 0.9392082620114763 1107 26-27 0.877527902074462 1107 28-29 0.7143742710355241 1107 30-31 0.89226402520368 1107 32-33 0.8597561944157306 1107 34-35 0.7444968409017818 1107 36-37 0.7739070241462116 1107 38-39 0.7866618192019672 1107 40-41 0.8126640545920694 1107 42-43 0.9811648993545674 1107 44-45 0.8063490512771452 1107 46-47 0.6800664126393059 1107 48-49 0.7421079135803623 1107 50-51 0.7355692494768817 1107 52-53 0.7745821632516936 1107 54-55 1.0006193410527615 1107 56-57 1.0293675902884516 1107 58-59 0.994392130380831 1107 60-61 1.054024857819492 1107 62-63 1.1722243432474997 1107 64-65 1.038066275960631 1107 66-67 0.9751681478096472 1107 68-69 0.9581127368184283 1107 70-71 1.0694862728021732 1107 72-73 1.1836366458427072 1107 74-75 0.9560505207132941 1107 76-77 1.0243612549131917 1107 78-79 1.1666190539819858 1107 80-81 1.2483316742995818 1107 82-83 1.1615777857617218 1107 84-85 1.109545136877852 1107 86-87 0.9796544661103468 1107 88-89 1.022399822181871 1107 90-91 0.9293013525072737 1107 92-93 1.0542482388738428 1107 94-95 0.970586785585283 1107 96-97 1.045869173786027 1107 98-99 1.2404236703256402 1107 100-101 1.4356099143687864 1108 1 0.16681297670289297 1108 2 0.09460656790498945 1108 3 -0.024618305141128616 1108 4 0.03941875082895763 1108 5 0.30396771289363755 1108 6 0.07913079182991822 1108 7 0.23069961838935882 1108 8 0.1281921841158109 1108 9 0.30697645031202114 1108 10-11 0.11705146232424823 1108 12-13 0.1729822886507293 1108 14-15 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-3.1595414862957583 2316 68-69 -2.5614816377337846 2316 70-71 -2.257886764662402 2316 72-73 -2.190854443524156 2316 74-75 -2.2221145451958257 2316 76-77 -3.020072334107134 2316 78-79 -3.0151692184858163 2316 80-81 -2.836524842044515 2316 82-83 -2.785816494932824 2316 84-85 -2.8972032627821207 2316 86-87 -2.6910126748548535 2316 88-89 -3.0783585544479024 2316 90-91 -3.138165442420405 2316 92-93 -3.5310682134399514 2316 94-95 -3.4081658092786 2316 96-97 -3.8757635154031433 2316 98-99 -3.9038207003019174 2316 100-101 -4.68750228562487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3491.0 3 1397.0 4 1340.0 5 1483.0 6 1754.0 7 1956.0 8 2501.0 9 3156.0 10 4114.0 11 5484.0 12 7260.0 13 9145.0 14 10501.0 15 13492.0 16 17139.0 17 21936.0 18 18647.0 19 11583.0 20 11309.0 21 11947.0 22 13094.0 23 14925.0 24 17121.0 25 19808.0 26 22857.0 27 27764.0 28 33113.0 29 40465.0 30 49282.0 31 61376.0 32 77820.0 33 100801.0 34 134984.0 35 185596.0 36 264456.0 37 354826.0 38 295119.0 39 47969.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.76764557196638 18.81352658413298 14.566646067085218 21.85218177681542 2 15.375424995431835 20.213201811856194 40.66478109514119 23.74659209757078 3 19.322292786716776 25.68366548507725 29.86242092730388 25.1316208009021 4 12.374244425096542 16.70237118255023 37.963394973526235 32.95998941882699 5 13.241793923839948 38.032062402060625 33.888416424807296 14.83772724929213 6 30.688647237567046 36.4221657157679 17.992872686513834 14.896314360151214 7 28.26449172734392 33.55934568538914 20.63113367879101 17.54502890847593 8 26.739713702411084 33.65389471667285 19.856356565616068 19.750035015299993 9 26.995530403409074 16.870449380582116 20.747622679623376 35.38639753638543 10-11 24.784494936330802 26.784756888415444 27.1145665409071 21.316181634346655 12-13 26.352893081679124 24.47983376969656 27.65831131988267 21.508961828741647 14-15 23.137889382350952 25.561757095180326 26.790651179015658 24.509702343453064 16-17 21.45909953449334 29.13216239442402 27.364793888145627 22.04394418293701 18-19 21.316133621215975 27.280438499452085 30.372642782960423 21.030785096371517 20-21 22.460752162038254 26.060803917142888 30.19444351156823 21.284000409250623 22-23 21.968370952005607 26.190853024262196 30.2017528423556 21.639023181376597 24-25 21.87404182572251 26.465027322119905 30.382108839748234 21.27882201240935 26-27 21.53124606320322 26.48054792923188 30.49571624453794 21.49248976302696 28-29 21.16557830321496 26.748936167997613 30.629720351889 21.455765176898428 30-31 21.354119759425394 26.42856138864002 30.486474836548055 21.73084401538653 32-33 21.176827938321804 26.62924003762618 30.359947381654273 21.83398464239775 34-35 21.92474563265897 26.57645804263059 30.004765768094238 21.494030556616206 36-37 21.961271930726454 26.11038069786322 30.204124979534402 21.72422239187592 38-39 21.569991704389956 26.49430955510073 30.29524510833619 21.64045363217312 40-41 21.603455590868037 26.577882240251544 30.299618156688506 21.519044012191916 42-43 21.571020490496547 26.668957125474197 29.69514774110007 22.064874642929183 44-45 21.648614875425594 26.752049257665472 29.604365017083545 21.99497084982539 46-47 22.07591044959484 26.987663549845024 29.028190907320973 21.90823509323916 48-49 22.106591949318524 27.101080547157952 28.665537924563417 22.126789578960107 50-51 21.90914902300478 27.384445120021322 28.69015071379787 22.016255143176025 52-53 22.184131736916587 27.56739226568517 28.34669565835273 21.90178033904552 54-55 21.680899393461733 27.349662295381506 28.602952602143645 22.36648570901312 56-57 22.018058969242073 27.6834872951864 28.17073745259267 22.127716282978856 58-59 22.19145079527365 27.19622962101517 28.294266346134826 22.318053237576354 60-61 21.917506936455304 27.254439071113634 28.90953196495594 21.918522027475127 62-63 21.871811556481 27.44453409682457 28.78602290473712 21.897631441957312 64-65 21.70920433698914 27.601532355629775 28.53504802796929 22.154215279411797 66-67 21.678478758406825 27.69823374328827 28.18798287439741 22.43530462390749 68-69 21.73298974439065 27.4869332083919 28.550419074392213 22.229657972825237 70-71 21.878654034574783 27.51223174245193 28.264097349988702 22.345016872984587 72-73 21.891343668342664 27.382360726860433 28.391108237527696 22.335187367269207 74-75 21.62408096536671 28.136469631610495 28.09492810791634 22.144521295106458 76-77 21.84376081813047 27.74103120046435 28.136249228903182 22.278958752502 78-79 21.882534402439546 27.741389886677382 28.098875313873766 22.27720039700931 80-81 21.904511732514344 28.020133485613602 27.894546521457176 22.18080826041488 82-83 21.90867068603313 27.973822320680476 28.02611416906067 22.09139282422573 84-85 21.64352126009995 27.91109310994769 27.93345284555618 22.511932784396187 86-87 21.92087260142112 27.7558115381672 27.96220164018831 22.361114220223367 88-89 21.8691466666389 27.706784374521714 27.748587391210645 22.675481567628744 90-91 21.699593785345293 27.64339533497416 28.136840063880214 22.52017081580033 92-93 21.873743162478487 27.64088489691622 28.186012343657545 22.299359596947742 94-95 21.50552580383019 27.408471146552888 28.27252059844958 22.813482451167346 96-97 21.500515110515803 27.305880901451214 28.359033330956034 22.834570657076952 98-99 21.32544269634021 27.63892421515593 27.985614825872386 23.05001826263148 100-101 21.22240480940008 27.5155520964963 28.327247720922312 22.934795373181313 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 306.0 1 239.0 2 258.0 3 376.0 4 349.5 5 335.0 6 542.5 7 1128.5 8 1974.0 9 2459.5 10 2294.0 11 2137.5 12 2208.0 13 2335.5 14 2604.5 15 2824.5 16 2981.0 17 3153.0 18 3324.0 19 3625.0 20 4058.0 21 4517.0 22 5212.5 23 6139.5 24 7252.5 25 8857.0 26 11381.5 27 14358.0 28 16791.5 29 19928.5 30 24066.0 31 28742.0 32 34663.5 33 39587.0 34 44127.5 35 48301.5 36 53019.5 37 57861.0 38 59063.5 39 58761.5 40 58766.5 41 60324.0 42 62081.0 43 64061.0 44 66879.0 45 69486.5 46 69734.5 47 71642.0 48 81700.0 49 79891.0 50 67376.5 51 61218.0 52 57801.5 53 56914.0 54 55972.0 55 52700.5 56 47814.5 57 42722.5 58 39364.5 59 35198.0 60 29518.5 61 24591.5 62 20587.5 63 15941.5 64 11466.0 65 8834.5 66 6565.5 67 5077.5 68 3720.5 69 2434.5 70 1910.5 71 1510.0 72 1210.5 73 1092.0 74 877.0 75 668.0 76 518.5 77 326.0 78 185.5 79 111.5 80 69.0 81 53.0 82 35.5 83 22.0 84 11.5 85 8.5 86 7.5 87 4.5 88 4.5 89 4.5 90 2.0 91 4.5 92 5.5 93 2.0 94 0.5 95 1.0 96 1.0 97 1.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014315266945892456 2 0.006402828488526443 3 0.008693271199869236 4 0.03300319724980297 5 0.04336224496701242 6 0.056636401589567245 7 0.025090758792436958 8 0.02274826056492728 9 0.014107044881224927 10-11 0.04307593962809457 12-13 0.041566329659255004 14-15 0.01853176375540987 16-17 0.022409899709842553 18-19 0.02954150542470534 20-21 0.023034565903845134 22-23 0.01353443420338923 24-25 0.002863053389178491 26-27 0.002498664776010319 28-29 0.0011712491137548372 30-31 0.006506939520860207 32-33 0.001197276871838278 34-35 0.0017959153077574171 36-37 0.006220634181942358 38-39 0.0067932448597780555 40-41 0.004216496809517414 42-43 7.027494682529024E-4 44-45 0.0013274156622554822 46-47 0.0027068868406778463 48-49 4.424718874184941E-4 50-51 0.0017698875496739764 52-53 8.068605005866656E-4 54-55 0.0022123594370924704 56-57 0.002160303920925589 58-59 0.0027068868406778463 60-61 8.328882586701065E-4 62-63 0.0013534434203389231 64-65 0.0032274420023466624 66-67 0.0022123594370924704 68-69 0.006558995037027088 70-71 0.010619325298043858 72-73 0.004138413535267092 74-75 0.0029411366634288136 76-77 0.003175386486179781 78-79 0.034773084799476946 80-81 0.023138676936178895 82-83 0.003982246986766447 84-85 0.00845902137711827 86-87 0.007756271908865367 88-89 0.005830217810690746 90-91 0.012675518186635685 92-93 0.014705683317144067 94-95 0.006715161585527733 96-97 0.004112385777183651 98-99 0.022774288323010723 100-101 0.04094166346525242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1921026.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.52138474320917 #Duplication Level Percentage of deduplicated Percentage of total 1 77.00007187341842 40.44150400118567 2 13.045094146159379 13.702928173236446 3 4.198947570537707 6.616036226063231 4 1.8223317359809428 3.828455449408614 5 1.0096393023353387 2.6513827124909803 6 0.5879856854893108 1.8529093446650207 7 0.3870274411982393 1.4229052001746743 8 0.28684451226154695 1.205237679197351 9 0.21268146813752795 1.0053292694221516 >10 1.2498987408775637 12.539850719959716 >50 0.1244774216869601 4.504676627846862 >100 0.0695156673837953 6.592440181726348 >500 0.004088396651996823 1.4828295225720045 >1k 0.0011966038981454116 1.269672451372624 >5k 1.994339830242353E-4 0.8838424406783155 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9428 0.49077940642136025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7448 0.38770948441093456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4322 0.2249839408732625 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3768 0.19614518491681007 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2711 0.14112250432841617 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2019 0.10510008714093406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.205551616688166E-5 2 0.0 0.0 0.0 0.0 1.0411103233376332E-4 3 0.0 0.0 0.0 0.0 1.0411103233376332E-4 4 0.0 0.0 0.0 0.0 1.0411103233376332E-4 5 0.0 0.0 0.0 0.0 1.0411103233376332E-4 6 0.0 0.0 0.0 0.0 1.0411103233376332E-4 7 0.0 0.0 0.0 0.0 1.0411103233376332E-4 8 0.0 0.0 0.0 0.0 2.0822206466752664E-4 9 0.0 0.0 0.0 0.0 2.0822206466752664E-4 10-11 0.0 0.0 0.0 0.0 2.3424982275096747E-4 12-13 0.0 0.0 0.0 0.0 2.602775808344083E-4 14-15 0.0 0.0 0.0 2.602775808344083E-5 2.602775808344083E-4 16-17 0.0 0.0 0.0 1.0411103233376332E-4 2.602775808344083E-4 18-19 0.0 0.0 0.0 1.0411103233376332E-4 2.602775808344083E-4 20-21 0.0 0.0 0.0 1.5616654850064498E-4 2.602775808344083E-4 22-23 0.0 0.0 0.0 2.0822206466752664E-4 3.383608550847308E-4 24-25 0.0 0.0 0.0 3.643886131681716E-4 4.684996455019349E-4 26-27 0.0 0.0 0.0 5.465829197522574E-4 4.684996455019349E-4 28-29 0.0 0.0 0.0 9.630270490873107E-4 4.684996455019349E-4 30-31 0.0 0.0 0.0 0.001665776517340213 4.945274035853758E-4 32-33 0.0 0.0 0.0 0.0025767480502606422 6.246661940025799E-4 34-35 0.0 0.0 0.0 0.003930191470599565 6.246661940025799E-4 36-37 0.0 0.0 0.0 0.0055178847136894554 6.246661940025799E-4 38-39 0.0 0.0 0.0 0.006663106069360852 6.506939520860207E-4 40-41 0.0 0.0 0.0 0.008875465506453323 6.767217101694616E-4 42-43 0.0 0.0 0.0 0.013065934557887295 6.767217101694616E-4 44-45 0.0 0.0 0.0 0.018375597206909226 6.767217101694616E-4 46-47 0.0 0.0 0.0 0.03120728194204555 7.287772263363432E-4 48-49 0.0 0.0 0.0 0.05697476244465197 7.808327425032249E-4 50-51 0.0 0.0 0.0 0.07743258029823646 7.808327425032249E-4 52-53 0.0 0.0 0.0 0.09440267856863989 7.808327425032249E-4 54-55 0.0 0.0 0.0 0.12040440889399727 7.808327425032249E-4 56-57 0.0 0.0 0.0 0.14086222674758175 7.808327425032249E-4 58-59 0.0 0.0 0.0 0.16933659409086604 8.068605005866657E-4 60-61 0.0 0.0 0.0 0.21912769530448833 8.589160167535474E-4 62-63 0.0 0.0 0.0 0.2565816391865597 8.849437748369882E-4 64-65 0.0 0.0 0.0 0.30095896671882627 8.849437748369882E-4 66-67 0.0 0.0 0.0 0.3890629278312735 8.849437748369882E-4 68-69 0.0 0.0 0.0 0.4827108014154936 8.849437748369882E-4 70-71 0.0 0.0 0.0 0.559961187407146 9.369992910038698E-4 72-73 0.0 0.0 0.0 0.648351453858511 0.0010931658395045148 74-75 0.0 0.0 0.0 0.7263045893184163 0.0011191935975879558 76-77 0.0 0.0 0.0 0.7845286841510735 0.0011452213556713965 78-79 0.0 0.0 0.0 0.8679736765665848 0.0011712491137548372 80-81 0.0 0.0 0.0 0.996290523917948 0.0012493323880051598 82-83 0.0 0.0 0.0 1.135513001906273 0.0013013879041720415 84-85 0.0 0.0 0.0 1.311174341211415 0.0013274156622554824 86-87 0.0 0.0 0.0 1.5931851000454964 0.0013534434203389231 88-89 0.0 0.0 0.0 1.9622326819106042 0.0013534434203389231 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17110 0.0 26.606758 1 GTACATG 11890 0.0 25.458572 1 GAGTACT 8835 0.0 24.22716 12-13 TACATGG 12150 0.0 24.169409 2 GGTATCA 12400 0.0 23.87497 1 TATCACG 120 1.7071229E-4 23.758764 2 ACATGGG 11985 0.0 23.47132 3 GTACTTT 9385 0.0 22.629566 14-15 TATCAAC 20020 0.0 22.572014 2 ATCAACG 20075 0.0 22.51017 3 TCAACGC 20040 0.0 22.50265 4 CAACGCA 20190 0.0 22.312511 5 AACGCAG 20425 0.0 22.102903 6 AGTACTT 9295 0.0 21.21353 12-13 CATGGGG 4635 0.0 21.119453 4 CGTATAG 160 4.975364E-5 20.79 1 TACCTGG 1670 0.0 20.202063 2 GTACCTG 1835 0.0 20.199238 1 ACGCAGA 22400 0.0 20.153574 7 CGCAGAG 22465 0.0 20.031803 8 >>END_MODULE