FastQCFastQC Report
Thu 26 May 2016
SRR936682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921026
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111190.5788052842595571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95350.4963493466512166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56210.2926040563740418No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49410.2572063053805623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.1475253328169426No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20730.10791108501394568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA196650.024.329861
TACCTGG17000.023.7500722
GGTATCA147650.023.4661671
GTACCTG18350.023.2751431
GTACATG111450.023.1223851
GAGTACT114200.022.97520312-13
AGGTAAG8550.022.2222882
TACATGG117650.022.0038912
GTACTTT119300.021.8736114-15
GTAAGGT8950.021.7564434
ACATGGG114700.021.4505623
AGTACTT118450.020.76767512-13
TAAGGTG10650.020.5116885
ATCAACG233100.020.3968963
TCAACGC233800.020.333714
TATCAAC235150.020.2201352
CAACGCA235650.020.1725045
AACGCAG239800.019.8233976
CCCTATA3951.8189894E-1219.2405642
ACTTTTT137600.019.08533916-17
Produced by FastQC (version 0.11.5)