##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936682_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1921026 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.588905095506256 31.0 31.0 34.0 30.0 34.0 2 31.800796553508384 31.0 31.0 34.0 30.0 34.0 3 32.06421724640895 33.0 31.0 34.0 30.0 34.0 4 35.863370927827106 37.0 35.0 37.0 35.0 37.0 5 35.69551999816765 37.0 35.0 37.0 33.0 37.0 6 35.80637169928986 37.0 35.0 37.0 35.0 37.0 7 35.74719238573554 37.0 35.0 37.0 33.0 37.0 8 35.72804428466871 37.0 35.0 37.0 33.0 37.0 9 37.36280091992508 39.0 37.0 39.0 34.0 39.0 10-11 37.25863965401822 39.0 37.0 39.0 33.5 39.0 12-13 37.13868786783729 39.0 37.0 39.0 33.0 39.0 14-15 38.257331238619365 40.0 38.0 41.0 33.0 41.0 16-17 38.18472732800076 40.0 37.5 41.0 33.0 41.0 18-19 38.27711311559552 40.0 37.5 41.0 33.5 41.0 20-21 38.36681127689058 40.0 38.0 41.0 34.0 41.0 22-23 38.285851675094456 40.0 38.0 41.0 34.0 41.0 24-25 38.17670999767833 40.0 37.5 41.0 33.0 41.0 26-27 37.97522964290957 40.0 37.0 41.0 33.0 41.0 28-29 37.89547278381448 40.0 37.0 41.0 33.0 41.0 30-31 37.84979302726772 40.0 37.0 41.0 33.0 41.0 32-33 37.65258044399191 40.0 36.5 41.0 33.0 41.0 34-35 37.47749561952831 40.0 36.0 41.0 33.0 41.0 36-37 37.22957211406821 39.0 36.0 41.0 31.5 41.0 38-39 37.06833067329646 39.0 36.0 41.0 31.0 41.0 40-41 36.81741267426885 39.0 35.0 41.0 31.0 41.0 42-43 36.551386602784135 39.0 35.0 41.0 30.5 41.0 44-45 36.21825810790692 39.0 35.0 40.0 30.0 41.0 46-47 35.72976003448157 39.0 35.0 40.0 28.5 41.0 48-49 35.20963146776775 38.5 35.0 40.0 26.5 41.0 50-51 34.98912898628129 38.0 34.5 40.0 26.0 41.0 52-53 34.716974939433406 38.0 34.0 40.0 25.0 41.0 54-55 34.443430229471126 38.0 34.0 40.0 24.0 41.0 56-57 34.17392138367727 38.0 34.0 40.0 23.5 41.0 58-59 33.81728826158522 37.5 33.0 40.0 22.5 41.0 60-61 33.464413808037996 37.0 33.0 40.0 22.0 41.0 62-63 33.17040008828616 36.5 32.5 39.0 22.0 41.0 64-65 32.73761495159357 36.0 32.0 39.0 20.0 40.0 66-67 32.341100016345436 35.5 31.0 39.0 19.0 40.0 68-69 31.89458523726384 35.0 31.0 38.0 18.0 40.0 70-71 31.430784382928707 35.0 31.0 37.5 16.0 39.5 72-73 31.027424928137357 35.0 30.0 37.0 13.0 39.0 74-75 30.659641514482367 34.0 30.0 36.5 9.0 39.0 76-77 29.24231634553619 32.5 28.5 35.0 8.0 37.5 78-79 30.03227598168895 34.0 30.0 36.0 7.0 37.5 80-81 29.872889799513388 34.0 30.0 35.0 6.0 37.0 82-83 29.535799879855865 34.0 29.5 35.0 2.0 37.0 84-85 29.096245183563365 34.0 29.0 35.0 2.0 36.0 86-87 28.71668603131868 34.0 29.0 35.0 2.0 36.0 88-89 28.302334533733536 33.5 28.5 35.0 2.0 35.5 90-91 27.865647315549086 33.0 27.0 35.0 2.0 35.0 92-93 27.43391422083824 33.0 26.5 35.0 2.0 35.0 94-95 26.927564488976202 33.0 25.5 34.5 2.0 35.0 96-97 26.27100856521463 32.5 25.0 34.0 2.0 35.0 98-99 25.437363419339455 32.0 22.0 34.0 2.0 35.0 100-101 22.64973613058855 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2748267513715916 1101 2 0.2774429318339777 1101 3 0.14327658489518313 1101 4 0.10663320278299437 1101 5 0.08625019145235058 1101 6 0.029790801081105656 1101 7 0.07996014500356097 1101 8 0.0625571274751735 1101 9 0.21778945941957062 1101 10-11 0.14103063872740762 1101 12-13 0.15555883296996598 1101 14-15 0.19079108553913215 1101 16-17 0.08071229390073853 1101 18-19 0.02799376731855574 1101 20-21 -0.18597258510208547 1101 22-23 -0.1517174912213477 1101 24-25 -0.07833970389322076 1101 26-27 -0.030474802059231365 1101 28-29 -0.08730789837950681 1101 30-31 -0.18925975056735922 1101 32-33 -0.11013112027072935 1101 34-35 0.05500787282665698 1101 36-37 0.04427558670164444 1101 38-39 0.06890385090135709 1101 40-41 0.19980880153353553 1101 42-43 0.29452961729528937 1101 44-45 0.40477996259780724 1101 46-47 0.29262388350261404 1101 48-49 0.41603478121454884 1101 50-51 0.3158713113195333 1101 52-53 0.41596755307229927 1101 54-55 0.302974567820236 1101 56-57 0.1006895887179482 1101 58-59 0.0028757722447849687 1101 60-61 0.1490080623446346 1101 62-63 0.07454134045377003 1101 64-65 0.14131620027767156 1101 66-67 0.07380244916981127 1101 68-69 -0.26625522414708414 1101 70-71 -0.03465573699730484 1101 72-73 0.05799028587853172 1101 74-75 -0.04895813246623959 1101 76-77 0.43440792181523946 1101 78-79 0.24530219949130583 1101 80-81 0.17222454776706186 1101 82-83 0.27982756657530317 1101 84-85 0.30654389765728496 1101 86-87 0.024716264731331705 1101 88-89 0.18922713078345765 1101 90-91 0.3919185447305509 1101 92-93 0.21883781428137894 1101 94-95 0.08747361304452639 1101 96-97 0.007890344322415643 1101 98-99 0.02930687657671527 1101 100-101 0.07538798368912936 1102 1 0.3635130931237356 1102 2 0.497284031087883 1102 3 0.19337806286102932 1102 4 0.05832203151447857 1102 5 0.08461307528121864 1102 6 0.07104681709037663 1102 7 0.11596013381549142 1102 8 -0.10889663555758489 1102 9 0.04070680516993974 1102 10-11 0.11029646667594761 1102 12-13 0.14040501956936424 1102 14-15 0.17565803461855722 1102 16-17 0.2817774413993348 1102 18-19 0.15251281468864164 1102 20-21 0.2216022130769275 1102 22-23 0.045831804607843196 1102 24-25 0.14816335443084228 1102 26-27 0.0012361747519804567 1102 28-29 -0.1769569522236054 1102 30-31 0.03679815157875055 1102 32-33 0.12033143981405203 1102 34-35 0.2017693454953715 1102 36-37 0.13911762725969368 1102 38-39 0.020570195910948996 1102 40-41 0.16086860887924104 1102 42-43 0.14992066515936386 1102 44-45 0.0668328404388987 1102 46-47 -0.08586988786095873 1102 48-49 -0.05523995114847935 1102 50-51 -0.18364932282040058 1102 52-53 -0.17728847068256215 1102 54-55 -0.027544104749431142 1102 56-57 -0.07892791691427448 1102 58-59 -0.002185324311071213 1102 60-61 0.03900984167840704 1102 62-63 -0.0034733017204828798 1102 64-65 -0.03458256230003798 1102 66-67 -0.09579248022377129 1102 68-69 -0.11525616157843288 1102 70-71 -0.2397507420179501 1102 72-73 -0.16229833626089984 1102 74-75 -0.5899156205891316 1102 76-77 -0.2578568387481859 1102 78-79 -0.08292291829004839 1102 80-81 0.09961761722971474 1102 82-83 0.03309120252232134 1102 84-85 0.2050025685960719 1102 86-87 0.13959893950676872 1102 88-89 -7.582752370609569E-4 1102 90-91 0.14112532117372112 1102 92-93 0.21523665356192723 1102 94-95 -0.1377235605902669 1102 96-97 -0.09013136785280906 1102 98-99 -0.39065742056385133 1102 100-101 -0.12982621733083377 1103 1 0.4540295157265284 1103 2 0.4728571261486927 1103 3 0.16135114689070207 1103 4 0.053266682792710185 1103 5 0.045159998973339555 1103 6 0.06772379786862359 1103 7 0.12436475586121531 1103 8 0.2298984335249017 1103 9 0.12360291945250168 1103 10-11 0.14247285453066638 1103 12-13 0.1792678198230817 1103 14-15 0.21035062445336905 1103 16-17 0.16765946340645144 1103 18-19 0.19381834956082145 1103 20-21 0.08844460573019575 1103 22-23 0.19525051892901502 1103 24-25 0.033077945865166214 1103 26-27 0.015709686238146503 1103 28-29 0.1134393413149013 1103 30-31 0.20407836999523 1103 32-33 0.066228353853937 1103 34-35 -0.057439391163846665 1103 36-37 -0.08975923769163074 1103 38-39 0.07706582482355628 1103 40-41 -0.01791200941589466 1103 42-43 0.15899664210429165 1103 44-45 0.15531269482791288 1103 46-47 0.22797253237013848 1103 48-49 0.046199832641271144 1103 50-51 -0.1341929923275842 1103 52-53 0.0295182914034271 1103 54-55 0.30512493361134574 1103 56-57 0.18877446305318557 1103 58-59 0.19652337098138872 1103 60-61 -0.014498997018812076 1103 62-63 -0.09898175432797274 1103 64-65 0.05903107218329495 1103 66-67 -0.0029779411873320782 1103 68-69 -0.10466572005724117 1103 70-71 -0.22685269728669866 1103 72-73 -0.27157783765357735 1103 74-75 -0.07349588588793665 1103 76-77 -0.2793868572253366 1103 78-79 -0.13871442983137072 1103 80-81 -0.1483279992728619 1103 82-83 -0.14883317904465443 1103 84-85 -0.011325497370116011 1103 86-87 -0.08969379924836574 1103 88-89 -0.23941633761813108 1103 90-91 -0.3104023256093278 1103 92-93 -0.11457179077220658 1103 94-95 -0.13051780233759658 1103 96-97 -0.5355606720639479 1103 98-99 -0.28697269041299833 1103 100-101 -0.23666298410505604 1104 1 0.1943580568800911 1104 2 0.30091921446989645 1104 3 0.1102160303319124 1104 4 0.03673813018374261 1104 5 0.025144648724186425 1104 6 0.13410354470406816 1104 7 0.07445124420533489 1104 8 0.056038991403362104 1104 9 0.10017523172697196 1104 10-11 0.023338768958751643 1104 12-13 0.09808470509715761 1104 14-15 0.1716730369626731 1104 16-17 0.02571880242930291 1104 18-19 -0.03963259264663321 1104 20-21 -0.07059187975007575 1104 22-23 0.016782794546173818 1104 24-25 0.08615246330602844 1104 26-27 0.1614565216811883 1104 28-29 -0.09213600664317312 1104 30-31 -0.07160820999216355 1104 32-33 -0.024967705485089198 1104 34-35 -0.050096931687356516 1104 36-37 -0.06144856697738277 1104 38-39 -0.1318334248471018 1104 40-41 0.025261482765785104 1104 42-43 0.0667676484620543 1104 44-45 0.24061299839627992 1104 46-47 0.2900001399078036 1104 48-49 0.21348593150145945 1104 50-51 0.09370471086274534 1104 52-53 -0.0365152729473337 1104 54-55 0.08484725447737418 1104 56-57 -0.026527902488943766 1104 58-59 -5.895220823575187E-4 1104 60-61 -0.25920682679142715 1104 62-63 -0.4592596625701404 1104 64-65 -0.36339896390124693 1104 66-67 -0.18727612550488004 1104 68-69 -0.2609249559814053 1104 70-71 -0.3013829682071396 1104 72-73 -0.3731245470871123 1104 74-75 -0.35677522605633527 1104 76-77 -0.2914408122055754 1104 78-79 -0.16860938372334644 1104 80-81 -0.2768654871014924 1104 82-83 -0.3188778551429401 1104 84-85 -0.08674974847269823 1104 86-87 -0.06259912028622949 1104 88-89 -0.32343489538203585 1104 90-91 -0.28631526449471423 1104 92-93 -0.24536416146926854 1104 94-95 -0.37125163589049137 1104 96-97 -0.38933076198891214 1104 98-99 -0.3871232144980006 1104 100-101 -0.39748823182829085 1105 1 0.4470472031149022 1105 2 0.44185107671618695 1105 3 0.26835327859549096 1105 4 0.17808341379461012 1105 5 -0.03581044522784538 1105 6 0.11116748053267855 1105 7 0.12723852436959504 1105 8 0.16739477505857536 1105 9 0.21253810410422602 1105 10-11 0.23845568566507325 1105 12-13 0.1478483348890549 1105 14-15 0.3182375864859779 1105 16-17 0.24054556123441984 1105 18-19 0.21752002611783183 1105 20-21 0.28631497503364756 1105 22-23 0.18937024007897918 1105 24-25 0.19385135140759502 1105 26-27 0.1436097841813435 1105 28-29 0.1563488302818996 1105 30-31 0.2592707419489102 1105 32-33 0.2714587508119308 1105 34-35 0.2171654368594318 1105 36-37 0.36389335204162165 1105 38-39 0.29421994131833884 1105 40-41 0.30158836507278153 1105 42-43 0.32701830483403427 1105 44-45 0.07245183177554537 1105 46-47 0.3276510482427071 1105 48-49 0.33410984013104184 1105 50-51 0.16274011823299617 1105 52-53 0.3629146156530041 1105 54-55 0.44147372660552264 1105 56-57 0.2389863660318028 1105 58-59 0.39358438382923566 1105 60-61 0.21219962601512066 1105 62-63 0.16713474045799614 1105 64-65 0.2598614000049224 1105 66-67 0.35287986834195806 1105 68-69 0.19194429319389528 1105 70-71 0.295763423010591 1105 72-73 0.0750349410524187 1105 74-75 0.084157971345455 1105 76-77 0.0634661703716013 1105 78-79 0.23963563066294924 1105 80-81 0.03577190278394582 1105 82-83 0.2988461434189098 1105 84-85 0.3804163935096412 1105 86-87 0.24259334714789915 1105 88-89 0.18006917255067734 1105 90-91 0.17322260957532976 1105 92-93 0.39479664847420537 1105 94-95 0.4689374030550404 1105 96-97 0.4018561626425132 1105 98-99 0.41504989413502 1105 100-101 0.40845979056798853 1106 1 0.3836190738886245 1106 2 0.43358170503571714 1106 3 0.22888843706914486 1106 4 0.11916502773788551 1106 5 -0.017997363521885745 1106 6 0.10827152400555917 1106 7 0.006608339951519326 1106 8 0.14522735729831027 1106 9 0.13568182117811034 1106 10-11 -0.009176497264029138 1106 12-13 0.05238749527946851 1106 14-15 0.06904080877296792 1106 16-17 0.06950277704374486 1106 18-19 0.04982681591587834 1106 20-21 0.1096139034342869 1106 22-23 0.20300005592521586 1106 24-25 0.09384944246981064 1106 26-27 0.052699191485523045 1106 28-29 0.17475221429018006 1106 30-31 0.04480047952870336 1106 32-33 0.19779798080515576 1106 34-35 0.11855778671780115 1106 36-37 0.24256836321379893 1106 38-39 0.3014937285187642 1106 40-41 0.34565350194606737 1106 42-43 0.19251259001269005 1106 44-45 0.21467136843228474 1106 46-47 0.11482969202560867 1106 48-49 -0.1896815207996596 1106 50-51 -0.08566702001962057 1106 52-53 -0.09337437990797781 1106 54-55 -0.17011873867016192 1106 56-57 -0.22182412000822893 1106 58-59 -0.1345073489527593 1106 60-61 -0.0069190223002948414 1106 62-63 -0.4873639161882224 1106 64-65 -0.1459878301302595 1106 66-67 0.04416742730489176 1106 68-69 0.006471652432981756 1106 70-71 0.08845400230468314 1106 72-73 0.06828011572267556 1106 74-75 0.29412583266454817 1106 76-77 0.11038590345314958 1106 78-79 0.15261437635377106 1106 80-81 0.2468724005272378 1106 82-83 0.24612287234142016 1106 84-85 0.28995868406857284 1106 86-87 0.3232188032739032 1106 88-89 0.2802520634754373 1106 90-91 0.20562401416196607 1106 92-93 0.48214890770208285 1106 94-95 0.5540065237346639 1106 96-97 0.5186317611312212 1106 98-99 0.4786308607525953 1106 100-101 0.8344595722638211 1107 1 0.5092121645737997 1107 2 0.49659828961241814 1107 3 0.35904060808148586 1107 4 0.16994395005119145 1107 5 0.0340711295947429 1107 6 0.18658725834402645 1107 7 0.20320324316326577 1107 8 0.12164545007613015 1107 9 0.1516779690411596 1107 10-11 0.23988761081460552 1107 12-13 0.23984103219260788 1107 14-15 0.35254084979723643 1107 16-17 0.37007015504628527 1107 18-19 0.3678846195182217 1107 20-21 0.30942610347025123 1107 22-23 0.35392915190790575 1107 24-25 0.4096127879943836 1107 26-27 0.27272682277767757 1107 28-29 0.35758202658138316 1107 30-31 0.2807471827939878 1107 32-33 0.26447359822033434 1107 34-35 0.2810109714024094 1107 36-37 0.2722214471196125 1107 38-39 0.2637460186257883 1107 40-41 0.4018542572897914 1107 42-43 0.33184507965510335 1107 44-45 0.1662233702091811 1107 46-47 0.2784547617461399 1107 48-49 0.27445125526670466 1107 50-51 0.2725891752658214 1107 52-53 0.25557883834193973 1107 54-55 0.3510236751975313 1107 56-57 0.378167758016275 1107 58-59 0.20199769868926154 1107 60-61 0.16181004600137783 1107 62-63 0.21642929500055175 1107 64-65 0.22377753594292216 1107 66-67 0.18784558822442676 1107 68-69 0.3339919718752533 1107 70-71 0.15219949274354505 1107 72-73 0.2849835384723285 1107 74-75 0.39121949147362045 1107 76-77 0.36006620426460145 1107 78-79 0.4278250297335475 1107 80-81 0.4384403810477586 1107 82-83 0.1757849069957338 1107 84-85 0.2623634435462172 1107 86-87 0.512453373385199 1107 88-89 0.8233105508155205 1107 90-91 0.7210876753661886 1107 92-93 1.0048224797054708 1107 94-95 1.1011628792219241 1107 96-97 1.023047463727682 1107 98-99 1.022480826317075 1107 100-101 0.6619707752029811 1108 1 0.3452920052437243 1108 2 0.38893931136493265 1108 3 0.06559890057466333 1108 4 0.04269689724101511 1108 5 -0.027313224939440772 1108 6 -0.11552263797658924 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2316 46-47 -1.36854070736684 2316 48-49 -1.1900994387289145 2316 50-51 -1.5844599196423204 2316 52-53 -1.43718510294525 2316 54-55 -1.211186422350984 2316 56-57 -1.1683771274923984 2316 58-59 -1.4920374962658016 2316 60-61 -1.2895855440243196 2316 62-63 -1.3147052304253606 2316 64-65 -1.2186422883996109 2316 66-67 -1.2109459525610724 2316 68-69 -1.35315336889321 2316 70-71 -1.3641322423373445 2316 72-73 -1.2806256428783875 2316 74-75 -1.3241040252161795 2316 76-77 -1.2826390342290672 2316 78-79 -1.0456289491134036 2316 80-81 -1.103083286281926 2316 82-83 -0.8119005328744002 2316 84-85 -1.0429757550562755 2316 86-87 -1.088203129264361 2316 88-89 -1.1209944431805994 2316 90-91 -1.276564045886559 2316 92-93 -1.6695765890661463 2316 94-95 -1.8225234892044888 2316 96-97 -1.965117451875571 2316 98-99 -2.3959622052521645 2316 100-101 -2.788545692475413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 27.0 5 43.0 6 114.0 7 301.0 8 727.0 9 1393.0 10 2501.0 11 4121.0 12 5929.0 13 8308.0 14 11016.0 15 15291.0 16 19686.0 17 26244.0 18 21914.0 19 13136.0 20 11271.0 21 11171.0 22 11609.0 23 13102.0 24 15394.0 25 18181.0 26 22497.0 27 27761.0 28 35034.0 29 43775.0 30 54113.0 31 67897.0 32 84909.0 33 109315.0 34 144784.0 35 198064.0 36 282995.0 37 377204.0 38 245998.0 39 15187.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70789081217751 19.767522575678218 15.207950761567604 21.31663585057667 2 15.537136127796886 21.289328706089865 39.846251401761144 23.327283764352106 3 19.69494968320016 25.90824543749405 30.122924897276267 24.273879982029527 4 12.924322900342883 17.57033391886362 37.311260195151306 32.19408298564218 5 13.075356606313502 38.464601728451356 33.64951853853097 14.810523126704167 6 30.694222774704766 37.11490630527645 17.24791856018607 14.942952359832715 7 28.19488127698428 34.01890448125117 20.287960704331955 17.4982535374326 8 27.01821838954809 34.02239220083435 19.285163240893148 19.67422616872442 9 27.03767214210528 17.052414908935305 20.387705615265844 35.52220733369357 10-11 24.96877312409049 27.17559381818475 26.63145623305101 21.224176824673748 12-13 26.660950962065527 24.78736589827399 27.45727146421464 21.094411675445844 14-15 23.146034837140288 25.849878541372064 26.780631897198603 24.223454724289045 16-17 21.273944236048862 29.34046702126884 27.419358197130073 21.96623054555222 18-19 21.231909406744105 27.119908840380084 30.881934966002543 20.766246786873268 20-21 22.339266188814257 25.841041672794034 30.963279769060847 20.856412369330858 22-23 21.951232208824408 25.94930300679099 30.938627585663 21.160837198721605 24-25 21.882837608652874 26.270336788778497 31.127142474906638 20.719683127661987 26-27 21.491432182594092 26.30635920596598 31.219098544215434 20.983110067224494 28-29 21.15137952323394 26.610493559170983 31.312824501073905 20.92530241652117 30-31 21.274444300713345 26.23395681108866 31.23477538429344 21.256823503904553 32-33 21.17232710819681 26.493448418841005 31.081390261113196 21.252834211848988 34-35 21.89148789582988 26.347786049172534 30.707468435248824 21.053257619748763 36-37 22.03540972410025 25.943566516803102 30.810457948447382 21.210565810649257 38-39 21.55515576856706 26.358820274405875 30.911550772687264 21.1744731843398 40-41 21.6324917892317 26.506322642979814 30.794506684963142 21.06667888282535 42-43 21.597269475318395 26.68167948509035 30.218455510533875 21.502595529057377 44-45 21.692514917457817 26.6848430820086 30.071799009623955 21.550842990909626 46-47 22.269431516403078 27.01337561000241 29.336753086223737 21.380439787370772 48-49 22.427084312184594 27.23437740374416 28.77777150156065 21.560766782510594 50-51 22.14147506242787 27.48502485922821 28.801150640520103 21.57234943782382 52-53 22.42364345847033 27.690412433601875 28.459938323404415 21.42600578452338 54-55 21.90302603400797 27.607975679067398 28.554188861027445 21.934809425897193 56-57 22.27546821027107 27.809476362971925 28.255139296346073 21.659916130410938 58-59 22.497153830577048 27.33616241099051 28.20047652190028 21.966207236532163 60-61 22.040401663585662 27.5823554553022 28.881088192008896 21.496154689103246 62-63 22.082974353902095 27.560718496789356 28.864392971204428 21.49191417810412 64-65 21.93380723831857 27.692515548298974 28.564042903959656 21.809634309422798 66-67 21.928945531362427 27.809703083378928 28.226964716391713 22.034386668866937 68-69 21.957166549888548 27.665456199876942 28.456649637536685 21.920727612697824 70-71 22.132558390736087 27.76435741234069 28.20113456385403 21.901949633069194 72-73 22.21532775471428 27.589203680326907 28.222225114196846 21.973243450761963 74-75 21.987052752018972 28.38662777078499 27.852043647509195 21.774275829686847 76-77 22.221820232831675 27.943054703497104 28.03006660848521 21.805058455186003 78-79 22.165463700692627 27.892454633674994 27.91564799813412 22.02643366749826 80-81 22.204436956642276 28.263985982584437 27.675857417414573 21.85571964335871 82-83 22.234893486755997 28.27703022812441 27.70976481600838 21.778311469111213 84-85 22.050992386282292 28.165835882084988 27.597553198412182 22.185618533220538 86-87 22.138777630879897 28.031551894995694 27.62821178596501 22.2014586881594 88-89 22.11334387229939 28.02846874655036 27.462910545839296 22.395276835310952 90-91 22.029180422359318 27.96455841069975 27.74894516909967 22.257315997841264 92-93 22.14792281966276 27.946104634531878 27.810627015408528 22.09534553039683 94-95 21.75830989347031 27.69909827458661 27.874721545880444 22.667870286062637 96-97 21.935810174122285 27.576094550596252 27.930211564351737 22.557883710929733 98-99 21.68920639035917 27.873847308794996 27.512206956260076 22.924739344585753 100-101 21.5042225906452 28.066750329745503 27.61792680599927 22.811100273610023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 587.0 1 440.0 2 375.0 3 462.5 4 418.5 5 392.5 6 622.5 7 1216.5 8 2008.0 9 2515.0 10 2505.0 11 2360.5 12 2460.0 13 2671.0 14 2984.0 15 3284.5 16 3564.5 17 3898.5 18 4205.5 19 4500.0 20 4905.0 21 5403.0 22 6057.5 23 7032.5 24 8146.0 25 9675.0 26 12250.5 27 15189.0 28 17688.0 29 20633.5 30 24710.5 31 29294.0 32 34813.5 33 39995.5 34 44439.5 35 48178.0 36 53006.0 37 57581.5 38 58555.0 39 58259.5 40 58201.5 41 59964.5 42 61652.5 43 63398.5 44 65832.0 45 67918.0 46 68129.0 47 70530.0 48 80944.5 49 78987.0 50 66525.5 51 60271.5 52 56862.5 53 56095.5 54 54971.0 55 51723.0 56 46793.5 57 42078.5 58 39136.5 59 35249.5 60 29695.5 61 24733.0 62 20669.5 63 16093.5 64 11516.0 65 8704.5 66 6629.0 67 5111.0 68 3720.5 69 2528.0 70 1931.5 71 1531.5 72 1333.5 73 1205.0 74 966.5 75 765.0 76 582.0 77 364.5 78 220.5 79 152.0 80 109.0 81 82.0 82 57.5 83 30.0 84 15.0 85 10.5 86 7.0 87 1.0 88 2.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0110222349931757 2 0.011868657686049017 3 0.005049385068187521 4 0.01353443420338923 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004372663358018059 10-11 2.3424982275096747E-4 12-13 1.5616654850064495E-4 14-15 7.808327425032248E-5 16-17 0.0 18-19 0.0 20-21 2.602775808344083E-5 22-23 0.0012753601460886005 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.602775808344083E-5 32-33 0.004034302502933329 34-35 7.548049844197841E-4 36-37 0.0029411366634288136 38-39 1.5616654850064495E-4 40-41 0.003800052680182361 42-43 0.01160838010521461 44-45 0.0020041373724249438 46-47 0.0015356377269230088 48-49 0.010124797894458483 50-51 4.6849964550193493E-4 52-53 0.001978109614341503 54-55 0.01100974166929547 56-57 0.0035137473412645117 58-59 0.001197276871838278 60-61 0.005986384359191391 62-63 0.003695941647848598 64-65 0.016527626382984925 66-67 0.002550720292177201 68-69 2.0822206466752664E-4 70-71 0.0012233046299217189 72-73 5.205551616688166E-5 74-75 0.0 76-77 0.0012753601460886005 78-79 0.011348102524380201 80-81 0.014185128155475252 82-83 0.012076879750716545 84-85 0.00786038294119913 86-87 0.009916575829790955 88-89 0.027797645633114803 90-91 0.02381539864634836 92-93 0.0023164704694262337 94-95 0.007964493973532894 96-97 0.009994659104041277 98-99 0.010150825652541922 100-101 0.011790574411798694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1921026.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.388995818549716 #Duplication Level Percentage of deduplicated Percentage of total 1 75.82072241542002 38.20530064750018 2 13.508333477255501 13.613427182020054 3 4.363753379762438 6.596554524180951 4 2.037328581349885 4.106357654666048 5 1.0595602278517986 2.669508794536293 6 0.6485507527027382 1.9607892699633331 7 0.44329076359627834 1.5635883503278207 8 0.31416803526473364 1.2664489452221315 9 0.23107901806569633 1.0479455707560255 >10 1.3625622736323628 13.046574577620246 >50 0.13063873155608133 4.567859947756922 >100 0.07314609269406033 6.852197678713661 >500 0.004993636980695023 1.7309134395234467 >1k 0.0015605115564671945 1.3951521935696725 >5k 2.0806820752895927E-4 0.7945038944067766 >10k+ 1.0403410376447963E-4 0.5828773292365367 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11119 0.5788052842595571 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9535 0.4963493466512166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5621 0.2926040563740418 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4941 0.2572063053805623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2834 0.1475253328169426 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2073 0.10791108501394568 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 1.0411103233376332E-4 16-17 0.0 0.0 0.0 0.0 1.5616654850064498E-4 18-19 0.0 0.0 0.0 0.0 1.5616654850064498E-4 20-21 0.0 0.0 0.0 5.205551616688166E-5 1.5616654850064498E-4 22-23 0.0 0.0 0.0 1.0411103233376332E-4 2.3424982275096747E-4 24-25 0.0 0.0 0.0 2.602775808344083E-4 2.602775808344083E-4 26-27 0.0 0.0 0.0 3.9041637125161244E-4 3.643886131681716E-4 28-29 0.0 0.0 0.0 8.068605005866657E-4 3.643886131681716E-4 30-31 0.0 0.0 0.0 0.0014575544526726865 4.164441293350533E-4 32-33 0.0 0.0 0.0 0.0023685259855931156 4.684996455019349E-4 34-35 0.0 0.0 0.0 0.0037219694059320383 4.684996455019349E-4 36-37 0.0 0.0 0.0 0.00536171816518881 4.684996455019349E-4 38-39 0.0 0.0 0.0 0.00661105055319397 5.205551616688166E-4 40-41 0.0 0.0 0.0 0.00882340999028644 5.205551616688166E-4 42-43 0.0 0.0 0.0 0.012831684735136328 5.205551616688166E-4 44-45 0.0 0.0 0.0 0.018141347384158256 5.205551616688166E-4 46-47 0.0 0.0 0.0 0.03118125418396211 5.205551616688166E-4 48-49 0.0 0.0 0.0 0.05713092899315262 5.205551616688166E-4 50-51 0.0 0.0 0.0 0.0774586080563199 5.205551616688166E-4 52-53 0.0 0.0 0.0 0.0942204842620558 6.506939520860207E-4 54-55 0.0 0.0 0.0 0.12027427010358008 7.808327425032249E-4 56-57 0.0 0.0 0.0 0.14109647657033275 8.328882586701065E-4 58-59 0.0 0.0 0.0 0.16933659409086604 8.849437748369882E-4 60-61 0.0 0.0 0.0 0.2195441394338234 9.109715329204289E-4 62-63 0.0 0.0 0.0 0.2574926107194801 9.890548071707515E-4 64-65 0.0 0.0 0.0 0.3021041880744977 0.0010411103233376332 66-67 0.0 0.0 0.0 0.39067664883244685 0.0010411103233376332 68-69 0.0 0.0 0.0 0.4850012441268364 0.0010931658395045148 70-71 0.0 0.0 0.0 0.562381768908906 0.0011452213556713965 72-73 0.0 0.0 0.0 0.650589841053687 0.0011452213556713965 74-75 0.0 0.0 0.0 0.7289854484010108 0.0011452213556713965 76-77 0.0 0.0 0.0 0.7873136542660016 0.0011452213556713965 78-79 0.0 0.0 0.0 0.8712271463270149 0.0011452213556713965 80-81 0.0 0.0 0.0 0.9998563267753795 0.0011452213556713965 82-83 0.0 0.0 0.0 1.1392609990702884 0.0011452213556713965 84-85 0.0 0.0 0.0 1.3145058942460954 0.0011972768718382782 86-87 0.0 0.0 0.0 1.5964125420478432 0.0012753601460886005 88-89 0.0 0.0 0.0 1.9657464292518685 0.0013013879041720415 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19665 0.0 24.32986 1 TACCTGG 1700 0.0 23.750072 2 GGTATCA 14765 0.0 23.466167 1 GTACCTG 1835 0.0 23.275143 1 GTACATG 11145 0.0 23.122385 1 GAGTACT 11420 0.0 22.975203 12-13 AGGTAAG 855 0.0 22.222288 2 TACATGG 11765 0.0 22.003891 2 GTACTTT 11930 0.0 21.87361 14-15 GTAAGGT 895 0.0 21.756443 4 ACATGGG 11470 0.0 21.450562 3 AGTACTT 11845 0.0 20.767675 12-13 TAAGGTG 1065 0.0 20.511688 5 ATCAACG 23310 0.0 20.396896 3 TCAACGC 23380 0.0 20.33371 4 TATCAAC 23515 0.0 20.220135 2 CAACGCA 23565 0.0 20.172504 5 AACGCAG 23980 0.0 19.823397 6 CCCTATA 395 1.8189894E-12 19.240564 2 ACTTTTT 13760 0.0 19.085339 16-17 >>END_MODULE