FastQCFastQC Report
Thu 26 May 2016
SRR936681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970049
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94280.478566776765451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76690.3892796575110568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.21796412170458704No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37730.19151807899194384No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26710.13558038404120912No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20940.10629177243814747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA174250.026.4832121
GTACATG117550.026.3602261
CGTAAGG1109.444209E-525.9235082
TACATGG121500.024.8389172
GGTATCA123200.024.765581
GAGTACT90800.024.62497912-13
ACATGGG119900.024.0605033
GTACTTT97200.022.70965614-15
ATCAACG202600.022.6606773
TCAACGC203550.022.5794124
TATCAAC203700.022.4916462
CAACGCA204900.022.4306455
AACGCAG208350.022.0358526
TACCTGG17350.021.9142062
AGTACTT94250.020.8747412-13
CATGGGA70000.020.8447934
TACGACG1404.8335994E-420.3695055
ACGCAGA226400.020.2585417
CGCAGAG226750.020.2472048
ACTTTTT112550.019.8433916-17
Produced by FastQC (version 0.11.5)