##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936681_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1970049 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.507947771857452 31.0 31.0 34.0 30.0 34.0 2 31.547232073923034 33.0 31.0 34.0 30.0 34.0 3 31.590703581484522 33.0 31.0 34.0 28.0 34.0 4 35.22231629771645 37.0 35.0 37.0 33.0 37.0 5 35.20209446567065 37.0 35.0 37.0 33.0 37.0 6 35.33716521771793 37.0 35.0 37.0 33.0 37.0 7 35.218303707166676 37.0 35.0 37.0 32.0 37.0 8 35.165785216509846 37.0 35.0 37.0 32.0 37.0 9 36.66103634985729 39.0 37.0 39.0 32.0 39.0 10-11 36.57557603897162 39.0 37.0 39.0 32.0 39.0 12-13 36.479844663762165 39.0 37.0 39.0 32.0 39.0 14-15 37.54654021295917 40.0 37.0 41.0 32.0 41.0 16-17 37.440273566799604 40.0 37.0 41.0 32.0 41.0 18-19 37.423024757252236 40.0 37.0 41.0 32.0 41.0 20-21 37.36014332638427 40.0 37.0 41.0 31.5 41.0 22-23 37.23195209865338 39.0 36.5 41.0 31.0 41.0 24-25 37.36348029922098 40.0 37.0 41.0 32.0 41.0 26-27 37.34477035850377 40.0 37.0 41.0 31.5 41.0 28-29 37.246635743577954 39.5 37.0 41.0 31.0 41.0 30-31 37.05176952451436 39.0 36.0 41.0 31.0 41.0 32-33 36.853473949125124 39.0 36.0 41.0 30.5 41.0 34-35 36.640752844218596 39.0 35.0 41.0 30.0 41.0 36-37 36.44616809023532 39.0 35.0 40.5 30.0 41.0 38-39 36.23977550812188 39.0 35.0 40.0 30.0 41.0 40-41 36.05887518533803 39.0 35.0 40.0 29.5 41.0 42-43 35.81220314824657 38.0 35.0 40.0 28.5 41.0 44-45 35.50578259728565 38.0 35.0 40.0 27.5 41.0 46-47 35.15265559384564 38.0 34.0 40.0 26.0 41.0 48-49 35.000001015203175 38.0 34.0 40.0 25.0 41.0 50-51 34.08016145791298 37.5 33.5 39.5 23.5 40.5 52-53 34.22044553206545 38.0 33.5 39.5 24.0 40.5 54-55 34.63220026506955 38.0 34.0 40.0 24.0 41.0 56-57 34.54820362336166 38.0 34.0 40.0 24.0 41.0 58-59 34.300053196646374 38.0 34.0 40.0 23.0 41.0 60-61 33.99656658286165 38.0 33.5 40.0 22.5 41.0 62-63 33.67498752569098 37.0 33.0 40.0 22.0 41.0 64-65 33.336907356111446 36.5 33.0 39.5 21.5 41.0 66-67 32.95932893039716 36.0 32.5 39.0 20.0 41.0 68-69 32.55293421635706 36.0 32.0 39.0 18.5 40.5 70-71 32.11854375195744 35.0 31.5 38.5 17.5 40.0 72-73 31.72816183759896 35.0 31.0 37.5 14.5 40.0 74-75 31.308444358490576 35.0 31.0 37.0 12.0 39.0 76-77 30.881899891830102 35.0 31.0 36.5 8.0 39.0 78-79 30.436435083594368 34.5 30.0 36.0 7.0 38.5 80-81 29.962542302247307 34.0 30.0 36.0 4.0 37.5 82-83 29.49135123035011 34.0 29.0 35.0 2.0 37.0 84-85 29.179191228238487 34.0 29.0 35.0 2.0 36.5 86-87 28.85943902918151 34.0 29.0 35.0 2.0 36.0 88-89 28.464814326953288 34.0 29.0 35.0 2.0 36.0 90-91 28.062147692773124 34.0 27.5 35.0 2.0 35.0 92-93 27.717205257331162 34.0 27.0 35.0 2.0 35.0 94-95 27.270529311707474 33.5 26.0 35.0 2.0 35.0 96-97 26.716138532594876 33.0 25.0 35.0 2.0 35.0 98-99 26.040713200534604 33.0 24.5 35.0 2.0 35.0 100-101 23.587990450998937 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29347807922287217 1101 2 0.4712104911214503 1101 3 0.4102030602743092 1101 4 0.5591160398429977 1101 5 0.4799899494480826 1101 6 0.3781136586994336 1101 7 0.48266625266914076 1101 8 0.47519035675373544 1101 9 0.7310824878827091 1101 10-11 0.47247001947531686 1101 12-13 0.6289232815748917 1101 14-15 0.7133528490137735 1101 16-17 0.7510439903787187 1101 18-19 0.7578623176181338 1101 20-21 0.7813443730563563 1101 22-23 0.7050859477627114 1101 24-25 0.6909137795552382 1101 26-27 0.5459750831575079 1101 28-29 0.562774184029017 1101 30-31 0.6547536813855857 1101 32-33 0.5513108025786693 1101 34-35 0.5140751426569139 1101 36-37 0.5925730859767029 1101 38-39 0.5458080315754756 1101 40-41 0.5541653200359278 1101 42-43 0.5789303324373236 1101 44-45 0.6420404583384212 1101 46-47 0.6871638004680776 1101 48-49 0.9019984934010594 1101 50-51 0.826749622228796 1101 52-53 0.8789126441025772 1101 54-55 0.9379083229912126 1101 56-57 0.7515245215477648 1101 58-59 0.8067672690869614 1101 60-61 0.6884314795847715 1101 62-63 0.8681782617380165 1101 64-65 0.8261942751665075 1101 66-67 0.8749132244021283 1101 68-69 0.9466135445992307 1101 70-71 0.9431498937305136 1101 72-73 0.9051865458284958 1101 74-75 0.7636535744387238 1101 76-77 0.8601598216286739 1101 78-79 0.6266222163589887 1101 80-81 0.8184219553427994 1101 82-83 0.908118947533314 1101 84-85 1.0488856243210378 1101 86-87 0.9676219639291048 1101 88-89 0.8755680132480528 1101 90-91 0.6107624870922557 1101 92-93 0.7085772608516372 1101 94-95 0.7624024857821325 1101 96-97 0.6007283859808688 1101 98-99 0.7529096187882978 1101 100-101 1.0338774258857768 1102 1 0.1970085522645597 1102 2 0.35527847091814024 1102 3 0.20423057388005716 1102 4 0.26918195165138314 1102 5 0.3168180128153324 1102 6 0.23118138744403893 1102 7 0.24980949277279763 1102 8 0.2773004707483935 1102 9 0.46343842015822645 1102 10-11 0.5015358929876186 1102 12-13 0.3264002081868327 1102 14-15 0.3933239993003568 1102 16-17 0.42810208553171236 1102 18-19 0.5882051526343659 1102 20-21 0.6471574626564163 1102 22-23 0.370385231626031 1102 24-25 0.37375415723968786 1102 26-27 0.30513571819160745 1102 28-29 0.38412637605406985 1102 30-31 0.22731347645900257 1102 32-33 0.37724043991479306 1102 34-35 0.20587510095673878 1102 36-37 0.23681122802111787 1102 38-39 0.10948632748502973 1102 40-41 0.27264871017511894 1102 42-43 0.4449725602479404 1102 44-45 0.22392556158891352 1102 46-47 0.35063614857093484 1102 48-49 0.2647698104883389 1102 50-51 0.28003882996800655 1102 52-53 0.4087557152779695 1102 54-55 0.4175841679516523 1102 56-57 0.6131988687059788 1102 58-59 0.7636665206113591 1102 60-61 0.5672239616388097 1102 62-63 0.5800496209509873 1102 64-65 0.49258776313090635 1102 66-67 0.6258812641960105 1102 68-69 0.5542857386667421 1102 70-71 0.5925035410066002 1102 72-73 0.4344081567318163 1102 74-75 0.35439166754925466 1102 76-77 0.38197305590281516 1102 78-79 0.326098411090296 1102 80-81 0.5670181912636707 1102 82-83 0.5853734164730682 1102 84-85 0.6743414540481538 1102 86-87 0.456122076689784 1102 88-89 0.42991972444454873 1102 90-91 0.3887190508275715 1102 92-93 0.20352409312757302 1102 94-95 0.4114942304238731 1102 96-97 0.4461041343500902 1102 98-99 0.702340283735893 1102 100-101 0.8689921651958485 1103 1 0.27804368589684003 1103 2 0.29904399843276863 1103 3 0.3163118092452919 1103 4 0.43089256724240954 1103 5 0.4651258644449854 1103 6 0.4437801641182091 1103 7 0.35636969234121807 1103 8 0.30935920875363365 1103 9 0.530510906550127 1103 10-11 0.48928477959785965 1103 12-13 0.4364198232320078 1103 14-15 0.4604022248147075 1103 16-17 0.6157981722154204 1103 18-19 0.5970606185745879 1103 20-21 0.6267976069252015 1103 22-23 0.7153211575122782 1103 24-25 0.5866245857098136 1103 26-27 0.6630694810663726 1103 28-29 0.4366119262191006 1103 30-31 0.37237234710173084 1103 32-33 0.667686586540448 1103 34-35 0.4851988282744557 1103 36-37 0.6347186164398195 1103 38-39 0.7260734116537293 1103 40-41 0.6422788046028103 1103 42-43 0.6421560540216262 1103 44-45 0.5314596264690508 1103 46-47 0.6001285550742566 1103 48-49 0.5446636239694129 1103 50-51 0.7603765964545914 1103 52-53 0.6592671348562789 1103 54-55 0.7325727152139692 1103 56-57 0.7573056827022953 1103 58-59 0.6460527712043955 1103 60-61 0.731372428910511 1103 62-63 0.7339901024251034 1103 64-65 0.6292770802008363 1103 66-67 0.5636570609137408 1103 68-69 0.5732898861392357 1103 70-71 0.6397428955721338 1103 72-73 0.7801188641488004 1103 74-75 0.7574291937579183 1103 76-77 0.735678360892539 1103 78-79 0.7718649792991314 1103 80-81 0.70033736809496 1103 82-83 0.6407540350739467 1103 84-85 0.6327398307692533 1103 86-87 0.5288348450894098 1103 88-89 0.7203594942674805 1103 90-91 0.8136736479921929 1103 92-93 0.8104292777232551 1103 94-95 0.6928967954290002 1103 96-97 0.9147053954265978 1103 98-99 0.9939393562937084 1103 100-101 1.3655168077122894 1104 1 0.17584317298048902 1104 2 0.20492634879341765 1104 3 0.15607195110009187 1104 4 0.34105938350236187 1104 5 0.407388776627144 1104 6 0.3741681766507057 1104 7 0.42917534301938787 1104 8 0.2984759678522835 1104 9 0.3965290577876246 1104 10-11 0.49306606806549524 1104 12-13 0.2764562301152296 1104 14-15 0.35533037616627183 1104 16-17 0.2693927542184227 1104 18-19 0.45880362607530856 1104 20-21 0.6390065690109807 1104 22-23 0.3700711393066456 1104 24-25 0.4177230128306846 1104 26-27 0.14379278436699394 1104 28-29 0.4156169904564777 1104 30-31 0.2851944442680576 1104 32-33 -0.005869377581731783 1104 34-35 -0.04915158589277979 1104 36-37 0.043300445724490544 1104 38-39 0.17918468888211692 1104 40-41 0.16144100321181298 1104 42-43 0.1977571602223378 1104 44-45 0.2461117409109974 1104 46-47 0.341136945459219 1104 48-49 0.20424358267990073 1104 50-51 0.21074606117382189 1104 52-53 0.2197828851433954 1104 54-55 0.2409609067319849 1104 56-57 0.1334863071687451 1104 58-59 0.22032233041745997 1104 60-61 0.2947893458498143 1104 62-63 0.3390988010336642 1104 64-65 0.17314388689735694 1104 66-67 0.19790881369964097 1104 68-69 0.14813522597921747 1104 70-71 0.08488405285390144 1104 72-73 0.0891878148110905 1104 74-75 -0.10976848522102856 1104 76-77 0.09420818347752657 1104 78-79 0.10597501328005876 1104 80-81 0.1614568216275991 1104 82-83 0.21299695615273606 1104 84-85 0.1639821262761032 1104 86-87 0.18504434851864815 1104 88-89 0.11318500444201973 1104 90-91 0.2226405374256366 1104 92-93 0.26365033712451336 1104 94-95 0.2163356731897892 1104 96-97 0.2652060942597707 1104 98-99 0.4704708669578217 1104 100-101 0.794651044220597 1105 1 0.32594715634694893 1105 2 0.3130481606605535 1105 3 0.35262531969353006 1105 4 0.41978503476629214 1105 5 0.3448183495867738 1105 6 0.41590466207701127 1105 7 0.49226491541405437 1105 8 0.5940733446371524 1105 9 0.6415359927814634 1105 10-11 0.6180777885967572 1105 12-13 0.36986856754403874 1105 14-15 0.22197945313437373 1105 16-17 0.30021899766887117 1105 18-19 0.39801934865466393 1105 20-21 0.3462718503879998 1105 22-23 0.2079178563591313 1105 24-25 0.4232578334311867 1105 26-27 0.38886689729190493 1105 28-29 0.26418950689667753 1105 30-31 0.13660671190778828 1105 32-33 0.13832698327342996 1105 34-35 -0.05773481282149362 1105 36-37 0.12457731541330475 1105 38-39 0.07210745823654463 1105 40-41 -0.1386213322111587 1105 42-43 0.20070871638843357 1105 44-45 0.06769949962021116 1105 46-47 0.22306567845733838 1105 48-49 0.21064518849357938 1105 50-51 0.3249909159496198 1105 52-53 0.13312727078935183 1105 54-55 0.2413954268833507 1105 56-57 0.3480020575450453 1105 58-59 0.22797254371332087 1105 60-61 0.03468411150209505 1105 62-63 -0.0017920495457630636 1105 64-65 0.10404082147103821 1105 66-67 0.18691798194427633 1105 68-69 0.3526959428413292 1105 70-71 0.46183449761691264 1105 72-73 0.3874889252884053 1105 74-75 0.3152616558312644 1105 76-77 0.10328847962063392 1105 78-79 0.347930930789353 1105 80-81 0.12664191819172288 1105 82-83 0.35998844117193585 1105 84-85 0.5815443602200432 1105 86-87 0.6297884824966076 1105 88-89 0.4345872523627641 1105 90-91 0.5425373350323 1105 92-93 0.5894241355000425 1105 94-95 0.6997509137496856 1105 96-97 0.7211408134524611 1105 98-99 0.7082648386524113 1105 100-101 1.1094179878360002 1106 1 0.005917210549949203 1106 2 0.10872331785329337 1106 3 0.17406152778303152 1106 4 0.15710900612968004 1106 5 0.19639930410022544 1106 6 0.19345676211553808 1106 7 0.2805733964591397 1106 8 0.1406607946350391 1106 9 0.3981994117059173 1106 10-11 0.2133096340203693 1106 12-13 0.4137910029876011 1106 14-15 0.4479931874529228 1106 16-17 0.46416643669301294 1106 18-19 0.5750916502549401 1106 20-21 0.2897102007770229 1106 22-23 0.3405136143236618 1106 24-25 0.3015233524950318 1106 26-27 0.12496489640029296 1106 28-29 0.27829499598446006 1106 30-31 0.3297177665364188 1106 32-33 0.2000882984274739 1106 34-35 0.18552120508345382 1106 36-37 0.11413738754949065 1106 38-39 0.1652253714512284 1106 40-41 0.15558162444459356 1106 42-43 0.04292429687696142 1106 44-45 0.15474895103418618 1106 46-47 -0.12865925593222727 1106 48-49 -0.34054217899954864 1106 50-51 -0.3808745871780985 1106 52-53 -0.11385331235184282 1106 54-55 -0.021895731188905643 1106 56-57 -0.1039907399309925 1106 58-59 -0.015108605462941682 1106 60-61 0.13682673882468777 1106 62-63 0.1643817823260818 1106 64-65 0.01966710115206638 1106 66-67 0.1821974088085554 1106 68-69 0.0973330076489134 1106 70-71 0.23920252530945874 1106 72-73 0.24641096232880244 1106 74-75 0.03054498099481151 1106 76-77 0.09637038525859509 1106 78-79 0.09077896368636118 1106 80-81 0.21697113536481538 1106 82-83 0.539702559167857 1106 84-85 0.35569755178237017 1106 86-87 0.4893453169767348 1106 88-89 0.42690992261203675 1106 90-91 0.5115038721927228 1106 92-93 0.6407177858614297 1106 94-95 0.6767430051948082 1106 96-97 0.8343152999158256 1106 98-99 1.101285521323792 1106 100-101 1.5391347534478577 1107 1 0.16392781341206586 1107 2 0.25123054888963736 1107 3 0.06468849003014654 1107 4 0.20478127024640003 1107 5 0.3624582348038743 1107 6 0.21828024708634786 1107 7 0.3775313278744292 1107 8 0.29122931057321466 1107 9 0.35519991204778734 1107 10-11 0.4269233431881858 1107 12-13 0.3136436413440862 1107 14-15 0.24974306336415708 1107 16-17 0.339496592556479 1107 18-19 0.4398710214188952 1107 20-21 0.503502194111114 1107 22-23 0.4802972463670727 1107 24-25 0.38595118366254155 1107 26-27 0.25997422548370963 1107 28-29 0.35743074012837184 1107 30-31 0.292662069117668 1107 32-33 0.17851902898640049 1107 34-35 0.41892258790079495 1107 36-37 0.3546181841894267 1107 38-39 0.19559622245100883 1107 40-41 0.04070744248360825 1107 42-43 0.3011481679681651 1107 44-45 0.5453486287023921 1107 46-47 0.43338164053687933 1107 48-49 0.35737903509693325 1107 50-51 0.15221674813666652 1107 52-53 0.42685365974264045 1107 54-55 0.3841078977846806 1107 56-57 0.3240555895331596 1107 58-59 0.2951947990544852 1107 60-61 0.28131278706083407 1107 62-63 0.2812700396413419 1107 64-65 0.3289949885332888 1107 66-67 0.2996364657737729 1107 68-69 0.21357540534295794 1107 70-71 0.34886136591170924 1107 72-73 0.28712491130190543 1107 74-75 0.33053942440933426 1107 76-77 0.3345440776420361 1107 78-79 0.46181372947254573 1107 80-81 0.43060353260037587 1107 82-83 0.5737038787946176 1107 84-85 0.5070944474700596 1107 86-87 0.4594588606928589 1107 88-89 0.4639957701134172 1107 90-91 0.5746958290477089 1107 92-93 0.47767162085227355 1107 94-95 0.31639656828672 1107 96-97 0.3915528646263766 1107 98-99 0.5301607288859316 1107 100-101 0.8406025641232802 1108 1 0.00889470549700988 1108 2 0.23570061130597253 1108 3 0.17698787016145445 1108 4 0.24146738154487224 1108 5 0.40814263557094677 1108 6 0.20604834338944045 1108 7 0.06472487661194037 1108 8 0.1806652192023961 1108 9 0.25642007090413443 1108 10-11 0.08437698819140138 1108 12-13 0.2805318105853942 1108 14-15 0.23601447626915473 1108 16-17 0.29494231469958976 1108 18-19 0.16364609424469023 1108 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-2.687744409567017 2316 60-61 -2.1357972411468076 2316 62-63 -2.0861752131460634 2316 64-65 -2.381175407708149 2316 66-67 -2.3572242492186106 2316 68-69 -2.96231879529482 2316 70-71 -3.0362893586059343 2316 72-73 -2.6929263502192846 2316 74-75 -2.165026960180917 2316 76-77 -2.329198749656335 2316 78-79 -3.0654381652250606 2316 80-81 -2.6912217272734473 2316 82-83 -2.5607678836537886 2316 84-85 -2.3983745218236443 2316 86-87 -3.0693635508073918 2316 88-89 -3.249575188440218 2316 90-91 -3.327093722621054 2316 92-93 -3.695397214075509 2316 94-95 -4.1918052751033485 2316 96-97 -5.382388888664895 2316 98-99 -6.8623156995573105 2316 100-101 -6.834570109896703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3791.0 3 1478.0 4 1427.0 5 1654.0 6 1828.0 7 2037.0 8 2647.0 9 3349.0 10 4284.0 11 5733.0 12 7668.0 13 9468.0 14 11171.0 15 14159.0 16 18113.0 17 23375.0 18 18843.0 19 12284.0 20 12077.0 21 12646.0 22 14115.0 23 15920.0 24 18278.0 25 21037.0 26 24463.0 27 29151.0 28 35489.0 29 42160.0 30 51837.0 31 64534.0 32 81070.0 33 104514.0 34 139834.0 35 192075.0 36 270647.0 37 361052.0 38 291833.0 39 43996.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.8004265420893 18.795475607124267 14.553514529089917 21.850583321696522 2 15.400120636326156 20.237200668577078 40.6424595187951 23.72021917630166 3 19.390520115699736 25.590011276510076 29.791869438220616 25.22759916956957 4 12.425947152739441 16.72008544295601 37.91221376178053 32.94175364252402 5 13.251287645963258 37.96479562477176 33.89100428004833 14.892912449216645 6 30.697289621660275 36.39102418253917 17.95326009230969 14.958426103490865 7 28.304028465022828 33.50516223102641 20.578279574588894 17.612529729361867 8 26.759916774710234 33.62569847438923 19.866711559976498 19.747673190924036 9 26.980017510324387 16.871258488546175 20.745468543372585 35.40325545775685 10-11 24.810656319476276 26.813960073083802 27.110510685141616 21.264872922298306 12-13 26.378263164830912 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21.743793117189917 38-39 21.633841928515768 26.420559395473152 30.260127271481185 21.685471404529892 40-41 21.67139169697539 26.52226770998043 30.286166786378715 21.520173806665465 42-43 21.5924976878243 26.681949192032917 29.7037673996932 22.021785720449582 44-45 21.64627793430614 26.739294360943376 29.60411451463362 22.01031319011686 46-47 22.076077565859002 26.98470387940968 29.00610748400288 21.933111070728444 48-49 22.119584087129727 27.141340123084547 28.624999238589506 22.114076551196227 50-51 21.98432267324182 27.345374528722093 28.648087541258626 22.022215256777457 52-53 22.231243757860177 27.514400545183893 28.323673747542806 21.930681949413128 54-55 21.743034853336276 27.340128103111965 28.537068553059232 22.379768490492523 56-57 22.037708605902573 27.692654097131363 28.117849420439732 22.151787876526324 58-59 22.25751026552927 27.169302989790182 28.221023184599815 22.35216356008073 60-61 21.98050281675054 27.26559695972684 28.87211185979654 21.88178836372608 62-63 21.961632158689824 27.381314370909436 28.722528860147893 21.934524610252844 64-65 21.71926224151334 27.543509715623752 28.502394737997566 22.234833304865337 66-67 21.73027652686762 27.600323671416653 28.197054772858916 22.47234502885681 68-69 21.7753776232581 27.483032845811113 28.50756058827591 22.234028942654877 70-71 21.898362868504336 27.46739176507705 28.247263431186592 22.38698193523202 72-73 21.91271017443904 27.365323003725127 28.326870983027668 22.395095838808164 74-75 21.65442624681722 28.05234683062129 28.081230431233678 22.211996491327813 76-77 21.845488921614464 27.707893276721812 28.109458418806476 22.337159382857248 78-79 21.953377566681887 27.65832212947506 28.05168606116507 22.33661424267798 80-81 21.919563094634654 28.004784109689318 27.877262000822906 22.198390794853122 82-83 21.91204864566732 27.93026716587514 27.981132214353032 22.176551974104513 84-85 21.71081757276802 27.850101843747527 27.88399052842994 22.555090055054517 86-87 21.939138618605934 27.729711180780036 27.93395473595296 22.397195464661067 88-89 21.934634643577986 27.654509440015595 27.73892945471676 22.671926461689658 90-91 21.7382409921783 27.564755477054582 28.11108400971894 22.58591952104818 92-93 21.96993446565406 27.560013920686107 28.13651410687909 22.33353750678075 94-95 21.494047502707758 27.38664034255193 28.244873156129536 22.874438998610774 96-97 21.552258051415343 27.25716301831621 28.32115806359907 22.869420866669373 98-99 21.369978815414864 27.62510519307928 27.904731562493257 23.1001844290126 100-101 21.375167414610612 27.41141148765734 28.18438140698095 23.029039690751098 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 286.0 1 224.5 2 265.0 3 369.0 4 326.5 5 318.5 6 548.5 7 1164.0 8 1910.0 9 2385.5 10 2317.0 11 2057.0 12 2102.0 13 2295.5 14 2554.0 15 2775.5 16 2969.5 17 3189.0 18 3398.0 19 3637.0 20 3999.0 21 4622.0 22 5434.0 23 6449.0 24 7483.5 25 9013.5 26 11739.0 27 14624.5 28 16902.0 29 20032.5 30 24421.0 31 29333.5 32 35332.0 33 40558.5 34 45272.5 35 49309.5 36 54110.5 37 59173.5 38 60208.5 39 59690.5 40 59980.5 41 62183.0 42 64144.5 43 65715.5 44 68551.5 45 71834.0 46 71854.0 47 73067.5 48 83143.5 49 81877.5 50 69462.0 51 62734.0 52 59150.5 53 58711.5 54 57865.0 55 54164.5 56 49289.5 57 44127.0 58 40638.5 59 36459.5 60 30504.5 61 25406.0 62 21191.5 63 16424.5 64 11950.0 65 9152.5 66 6804.0 67 5265.0 68 3954.5 69 2654.5 70 1979.0 71 1564.0 72 1293.0 73 1167.0 74 956.5 75 748.5 76 524.5 77 307.0 78 195.0 79 139.0 80 78.0 81 42.5 82 34.5 83 26.0 84 18.0 85 13.0 86 12.0 87 6.5 88 6.0 89 6.5 90 4.5 91 3.0 92 2.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03675035494041011 2 0.025024758267433958 3 0.023755754298497144 4 0.051572321297592094 5 0.058171141936063514 6 0.05202916272640934 7 0.05258752447274154 8 0.04751150859699429 9 0.04695314685066209 10-11 0.05464331090241918 12-13 0.049110453597854674 14-15 0.04398367756334995 16-17 0.050379457566791484 18-19 0.05426260971173814 20-21 0.012613899451231923 22-23 0.02223294953577297 24-25 0.0061419792096541765 26-27 0.011573316196703737 28-29 0.006471920241577747 30-31 0.009948991116464617 32-33 0.004086192779976539 34-35 0.008350046115604233 36-37 0.01012665167211577 38-39 0.012385478736823295 40-41 0.0022334469853287913 42-43 0.002182686826571319 44-45 0.0024111075409799453 46-47 3.553211113023077E-4 48-49 0.001065963333906923 50-51 3.299410319235714E-4 52-53 0.003629351351159286 54-55 0.007690164051757088 56-57 0.018578218105234946 58-59 0.011598696276082473 60-61 0.011471795879188791 62-63 0.005989698733381759 64-65 2.538007937873626E-5 66-67 0.007614023813620879 68-69 0.018324417311447582 70-71 0.005558237383943242 72-73 0.0024618676997374177 74-75 0.0037054915892954943 76-77 0.006700340955986373 78-79 0.005964318654003022 80-81 0.009872850878328407 82-83 0.006624200717850166 84-85 0.018527457946477473 86-87 0.004923735399474835 88-89 0.006954141749773737 90-91 0.004086192779976539 92-93 0.016167110564255 94-95 0.012461618974959504 96-97 0.01060887318031176 98-99 0.02317201247278621 100-101 0.03865386089381533 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1970049.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.485218517705704 #Duplication Level Percentage of deduplicated Percentage of total 1 77.03684255926456 40.4329551563709 2 12.933027096408795 13.575855065008488 3 4.228775756300219 6.658446588953796 4 1.882058732456894 3.9512105534462516 5 0.9842793176911686 2.583005753573963 6 0.6048477142749904 1.9047338672194614 7 0.3847062900280322 1.4133975588079994 8 0.27787826607302446 1.1667601212931085 9 0.21067534380917854 0.9951607310505759 >10 1.2587957415152582 12.563193771001218 >50 0.12181847940304241 4.417634946709077 >100 0.0708601131506292 6.713217934528065 >500 0.003978895974904853 1.4593876961377057 >1k 0.0012616011627747093 1.2942057088916294 >5k 1.9409248658072454E-4 0.8708345470077604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9428 0.478566776765451 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7669 0.3892796575110568 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4294 0.21796412170458704 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3773 0.19151807899194384 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2671 0.13558038404120912 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2094 0.10629177243814747 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.076015875747253E-5 2 0.0 0.0 0.0 0.0 5.076015875747253E-5 3 0.0 0.0 0.0 0.0 5.076015875747253E-5 4 0.0 0.0 0.0 0.0 5.076015875747253E-5 5 0.0 0.0 0.0 0.0 5.076015875747253E-5 6 0.0 0.0 0.0 0.0 5.076015875747253E-5 7 0.0 0.0 0.0 0.0 5.076015875747253E-5 8 0.0 0.0 0.0 0.0 1.0152031751494506E-4 9 0.0 0.0 0.0 5.076015875747253E-5 1.0152031751494506E-4 10-11 0.0 0.0 0.0 1.2690039689368133E-4 1.0152031751494506E-4 12-13 0.0 0.0 0.0 1.5228047627241758E-4 1.0152031751494506E-4 14-15 0.0 0.0 0.0 1.7766055565115384E-4 1.0152031751494506E-4 16-17 0.0 0.0 0.0 2.0304063502989012E-4 1.0152031751494506E-4 18-19 0.0 0.0 0.0 2.5380079378736265E-4 1.0152031751494506E-4 20-21 0.0 0.0 0.0 3.2994103192357147E-4 1.0152031751494506E-4 22-23 0.0 0.0 0.0 4.0608127005978023E-4 1.7766055565115386E-4 24-25 0.0 0.0 0.0 5.837418257109341E-4 2.5380079378736265E-4 26-27 0.0 0.0 0.0 7.61402381362088E-4 3.553211113023077E-4 28-29 0.0 0.0 0.0 0.0011167234926643956 3.553211113023077E-4 30-31 0.0 0.0 0.0 0.002385727461601209 4.314613494385165E-4 32-33 0.0 0.0 0.0 0.0036039712717805495 6.598820638471429E-4 34-35 0.0 0.0 0.0 0.004796835002581154 6.598820638471429E-4 36-37 0.0 0.0 0.0 0.006192739368411649 6.598820638471429E-4 38-39 0.0 0.0 0.0 0.007537883575484671 6.598820638471429E-4 40-41 0.0 0.0 0.0 0.009872850878328407 6.598820638471429E-4 42-43 0.0 0.0 0.0 0.013959043658304946 6.598820638471429E-4 44-45 0.0 0.0 0.0 0.02045634397926143 6.598820638471429E-4 46-47 0.0 0.0 0.0 0.03657269438475896 7.360223019833518E-4 48-49 0.0 0.0 0.0 0.06489686297142863 8.121625401195605E-4 50-51 0.0 0.0 0.0 0.08613998941143089 8.121625401195605E-4 52-53 0.0 0.0 0.0 0.10532732942175549 8.375426194982968E-4 54-55 0.0 0.0 0.0 0.13007290681602335 8.62922698877033E-4 56-57 0.0 0.0 0.0 0.15365100055886935 9.136828576345055E-4 58-59 0.0 0.0 0.0 0.18710194518004375 9.390629370132418E-4 60-61 0.0 0.0 0.0 0.2408061931454497 9.644430163919781E-4 62-63 0.0 0.0 0.0 0.28009455602373345 9.644430163919781E-4 64-65 0.0 0.0 0.0 0.32608325985800357 9.644430163919781E-4 66-67 2.5380079378736265E-5 0.0 0.0 0.41384757434967356 0.0010152031751494506 68-69 5.076015875747253E-5 0.0 0.0 0.5142511683719542 0.0010913434132856594 70-71 5.076015875747253E-5 0.0 0.0 0.593183215239824 0.0011674836514218682 72-73 5.076015875747253E-5 0.0 0.0 0.6840946595744573 0.001421284445209231 74-75 5.076015875747253E-5 0.0 0.0 0.7614023813620879 0.001421284445209231 76-77 5.076015875747253E-5 0.0 0.0 0.8253348013171247 0.001421284445209231 78-79 5.076015875747253E-5 0.0 0.0 0.9116778313635854 0.001421284445209231 80-81 5.076015875747253E-5 0.0 0.0 1.0402025533375059 0.001421284445209231 82-83 5.076015875747253E-5 0.0 0.0 1.1889044384175216 0.001421284445209231 84-85 5.076015875747253E-5 0.0 0.0 1.3687476808952468 0.0014720446039667035 86-87 5.076015875747253E-5 0.0 0.0 1.6561009396212987 0.001522804762724176 88-89 5.076015875747253E-5 0.0 0.0 2.019467536086666 0.001522804762724176 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17425 0.0 26.483212 1 GTACATG 11755 0.0 26.360226 1 CGTAAGG 110 9.444209E-5 25.923508 2 TACATGG 12150 0.0 24.838917 2 GGTATCA 12320 0.0 24.76558 1 GAGTACT 9080 0.0 24.624979 12-13 ACATGGG 11990 0.0 24.060503 3 GTACTTT 9720 0.0 22.709656 14-15 ATCAACG 20260 0.0 22.660677 3 TCAACGC 20355 0.0 22.579412 4 TATCAAC 20370 0.0 22.491646 2 CAACGCA 20490 0.0 22.430645 5 AACGCAG 20835 0.0 22.035852 6 TACCTGG 1735 0.0 21.914206 2 AGTACTT 9425 0.0 20.87474 12-13 CATGGGA 7000 0.0 20.844793 4 TACGACG 140 4.8335994E-4 20.369505 5 ACGCAGA 22640 0.0 20.258541 7 CGCAGAG 22675 0.0 20.247204 8 ACTTTTT 11255 0.0 19.84339 16-17 >>END_MODULE