FastQCFastQC Report
Thu 26 May 2016
SRR936681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970049
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111110.5639961239542772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97430.4945562267740548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58450.2966931279374269No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49360.2505521436268844No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28660.1454786149989163No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21090.10705317481950957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG8850.027.3698885
GTAAGGT7250.026.8591354
TATCACG908.944923E-426.3913652
GTATCAA198000.025.4660131
GGTATCA143900.023.7453651
GTACATG119650.023.4341981
GAGTACT112150.022.99716212-13
TACATGG125400.021.9717582
AGTACTT114850.021.96024712-13
ACATGGG121050.021.8172633
GTACTTT118050.021.74692714-15
TATCAAC234900.021.3962172
TCAACGC235000.021.342354
ATCAACG235300.021.276933
CAACGCA235950.021.2362885
AACGCAG241550.020.783816
TACCTGG17850.019.6937242
GTATAGG6550.019.5899471
AGGTAAG8050.019.4738772
CATGGGG44750.019.3163264
Produced by FastQC (version 0.11.5)