FastQCFastQC Report
Thu 26 May 2016
SRR936680_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936680_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991708
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93490.4693961162981722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72960.36631875756888055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.20871533377382628No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37790.18973664814320174No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26650.1338047545122076No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.10925296278370122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA173700.028.6698971
GTACATG122250.025.1100941
ATCAACG204100.024.262363
TCAACGC204300.024.2392184
TATCAAC205500.024.0934372
CAACGCA206050.024.0339555
TACATGG125350.023.9571762
AACGCAG209200.023.7623426
ACATGGG123500.023.473143
GAGTACT90950.023.4125712-13
GGTATCA121800.022.7840161
AGTACTT94950.022.30111312-13
ACGCAGA226400.021.8311277
CGCAGAG227350.021.7442748
GTACTTT96400.021.66711614-15
TACCTGG17400.021.5735052
GCAGAGT230000.020.978659
GTACCTG18400.020.660311
CATGGGG47550.020.2891924
AGAGTAC177200.019.81040410-11
Produced by FastQC (version 0.11.5)