##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936680_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1991708 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.476182251615196 31.0 31.0 34.0 30.0 34.0 2 31.534047661605015 33.0 31.0 34.0 30.0 34.0 3 31.478162963647282 33.0 31.0 34.0 28.0 34.0 4 35.14834252812159 37.0 35.0 37.0 32.0 37.0 5 35.183297953314444 37.0 35.0 37.0 33.0 37.0 6 35.25823966163715 37.0 35.0 37.0 32.0 37.0 7 35.13559216511657 37.0 35.0 37.0 32.0 37.0 8 35.11644879671116 37.0 35.0 37.0 32.0 37.0 9 36.62111213089469 39.0 37.0 39.0 32.0 39.0 10-11 36.503308968985415 39.0 37.0 39.0 32.0 39.0 12-13 36.416510602959875 39.0 37.0 39.0 32.0 39.0 14-15 37.41998249743436 40.0 37.0 41.0 32.0 41.0 16-17 37.346483771717544 40.0 37.0 41.0 32.0 41.0 18-19 37.260285895322 40.0 37.0 41.0 31.0 41.0 20-21 37.270978978846294 39.5 37.0 41.0 31.0 41.0 22-23 37.270666935112985 39.5 37.0 41.0 31.5 41.0 24-25 37.380466413751414 40.0 37.0 41.0 32.0 41.0 26-27 37.30860748663961 40.0 37.0 41.0 31.0 41.0 28-29 37.20054897605472 39.5 36.5 41.0 31.0 41.0 30-31 36.99528369620447 39.0 36.0 41.0 31.0 41.0 32-33 36.81068259001822 39.0 36.0 41.0 30.0 41.0 34-35 36.616703101056984 39.0 35.0 41.0 30.0 41.0 36-37 36.408468259403485 39.0 35.0 40.5 30.0 41.0 38-39 36.21983794813296 39.0 35.0 40.0 30.0 41.0 40-41 35.97613154137052 38.5 35.0 40.0 29.5 41.0 42-43 35.69319122080144 38.0 35.0 40.0 28.0 41.0 44-45 35.49299721645944 38.0 35.0 40.0 27.0 41.0 46-47 35.270111381788894 38.0 35.0 40.0 26.0 41.0 48-49 34.99942561861478 38.0 34.5 40.0 24.5 41.0 50-51 34.01147005484739 37.5 33.0 39.5 23.5 40.5 52-53 34.11489485406496 38.0 33.5 39.5 24.0 40.5 54-55 34.55810540500917 38.0 34.0 40.0 24.0 41.0 56-57 34.4229636071151 38.0 34.0 40.0 23.0 41.0 58-59 34.20405375687601 38.0 34.0 40.0 23.0 41.0 60-61 33.92988329614582 38.0 33.5 40.0 22.0 41.0 62-63 33.6012153890028 37.0 33.0 40.0 22.0 41.0 64-65 33.24963599081793 36.5 33.0 39.5 20.5 41.0 66-67 32.892382066045826 36.0 32.0 39.0 20.0 41.0 68-69 32.46421990572915 35.5 32.0 39.0 18.5 40.5 70-71 32.064901079877174 35.0 31.0 38.5 17.5 40.0 72-73 31.652498759858375 35.0 31.0 37.5 13.5 40.0 74-75 31.24440003253489 35.0 31.0 37.0 11.0 39.0 76-77 30.791678549265256 35.0 30.5 36.5 7.5 39.0 78-79 30.327130784231425 34.5 30.0 36.0 7.0 38.5 80-81 29.879465765061948 34.0 29.5 35.5 2.0 37.0 82-83 29.49081316136703 34.0 29.0 35.0 2.0 37.0 84-85 29.125695132017345 34.0 29.0 35.0 2.0 36.5 86-87 28.78885208072669 34.0 29.0 35.0 2.0 36.0 88-89 28.43819224504797 34.0 29.0 35.0 2.0 36.0 90-91 28.085772111172922 34.0 27.5 35.0 2.0 35.5 92-93 27.690741564526526 34.0 27.0 35.0 2.0 35.0 94-95 27.231917781120526 33.5 26.0 35.0 2.0 35.0 96-97 26.664274331377893 33.0 25.0 35.0 2.0 35.0 98-99 25.95886269473236 33.0 24.5 35.0 2.0 35.0 100-101 23.41079867129117 30.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42482942348954467 1101 2 0.3855762702765091 1101 3 0.4643246741176874 1101 4 0.5922197507025047 1101 5 0.43687145710506314 1101 6 0.4091714763255965 1101 7 0.4593356102663435 1101 8 0.4033626696458299 1101 9 0.5367532275696689 1101 10-11 0.6629781925395264 1101 12-13 0.6117628038804384 1101 14-15 0.8383074501022065 1101 16-17 0.902690601084501 1101 18-19 1.01181181335987 1101 20-21 0.8180354665012572 1101 22-23 0.8635486205548162 1101 24-25 0.877028718276236 1101 26-27 0.728173828182122 1101 28-29 0.6434647265503202 1101 30-31 0.44918903407340594 1101 32-33 0.3680823151859727 1101 34-35 0.5719979585835873 1101 36-37 0.7713067920674561 1101 38-39 0.6820582770867247 1101 40-41 0.6569367846051648 1101 42-43 0.8099953164441303 1101 44-45 0.7835504492380494 1101 46-47 0.9880801890683699 1101 48-49 0.9439133819562144 1101 50-51 0.8230735017763848 1101 52-53 0.8172079708342679 1101 54-55 0.7982240749845175 1101 56-57 0.928545216722803 1101 58-59 0.84332906397389 1101 60-61 0.8109408581867825 1101 62-63 0.7647218143121677 1101 64-65 0.5285321445664763 1101 66-67 0.6010047249697053 1101 68-69 0.7278505053503537 1101 70-71 0.6148054735715505 1101 72-73 0.6753319858721767 1101 74-75 0.5927719786026344 1101 76-77 0.6737060159547141 1101 78-79 0.7371269472845547 1101 80-81 0.6683654923424776 1101 82-83 0.6865176968762441 1101 84-85 0.7169933707170735 1101 86-87 0.7617776765060533 1101 88-89 0.8170012530106412 1101 90-91 0.7538057639062963 1101 92-93 0.939640661985397 1101 94-95 1.0259228018326674 1101 96-97 1.018096943558234 1101 98-99 0.9429587306928013 1101 100-101 1.220123391274349 1102 1 0.26637231318160204 1102 2 0.43929351134294237 1102 3 0.4546872592442881 1102 4 0.3057737704927064 1102 5 0.3924637769340791 1102 6 0.3842025528903861 1102 7 0.43016527641380264 1102 8 0.3488882996204765 1102 9 0.5373599388978718 1102 10-11 0.5896470771605351 1102 12-13 0.5202005070878997 1102 14-15 0.6511711820120141 1102 16-17 0.4391611300162026 1102 18-19 0.5564057008793455 1102 20-21 0.718283171674635 1102 22-23 0.4167089534496 1102 24-25 0.49197460523113534 1102 26-27 0.4378157797803084 1102 28-29 0.28241835618219113 1102 30-31 0.354845019273597 1102 32-33 0.44138096777871283 1102 34-35 0.13609885534285837 1102 36-37 0.38977237092833406 1102 38-39 0.4348342989839864 1102 40-41 0.45193922939574094 1102 42-43 0.3661174249121828 1102 44-45 0.34756575312299987 1102 46-47 0.5574637489859029 1102 48-49 0.5488944033241836 1102 50-51 0.3714649303910207 1102 52-53 0.29429075598002186 1102 54-55 0.5641673860878953 1102 56-57 0.44056037946520377 1102 58-59 0.24088134972706854 1102 60-61 0.4479406716769887 1102 62-63 0.39766206564672046 1102 64-65 0.47569230607388135 1102 66-67 0.4988709520781427 1102 68-69 0.5375443772433215 1102 70-71 0.31035252696880633 1102 72-73 0.42599197851259873 1102 74-75 0.35085294401767086 1102 76-77 0.5484197234166146 1102 78-79 0.4046160717470819 1102 80-81 0.5733335084327251 1102 82-83 0.42969471483663213 1102 84-85 0.4730437989334888 1102 86-87 0.32065811364469354 1102 88-89 0.44074062321242735 1102 90-91 0.6954568292805021 1102 92-93 0.6840736469990851 1102 94-95 0.7845049241125217 1102 96-97 0.6228058622550847 1102 98-99 0.6325410999721193 1102 100-101 1.1067579984191873 1103 1 0.47618358385019377 1103 2 0.43630140867255207 1103 3 0.3974901731394027 1103 4 0.44074490210295636 1103 5 0.4947316939687312 1103 6 0.3506112843658684 1103 7 0.5823124618096145 1103 8 0.46586230020243846 1103 9 0.4688066255418377 1103 10-11 0.44347245786082823 1103 12-13 0.4490718576997281 1103 14-15 0.7264731132077387 1103 16-17 0.7548162203990287 1103 18-19 0.8603124031443983 1103 20-21 0.7773076191933868 1103 22-23 0.5863892315662866 1103 24-25 0.45257719138962216 1103 26-27 0.41816628440282244 1103 28-29 0.44887262364999714 1103 30-31 0.4451417689634738 1103 32-33 0.517162966767934 1103 34-35 0.4694861307896687 1103 36-37 0.42946280785945845 1103 38-39 0.27632749155733194 1103 40-41 0.5550117857924803 1103 42-43 0.47133190862489727 1103 44-45 0.49710717709270114 1103 46-47 0.4433954756270069 1103 48-49 0.5922911228949559 1103 50-51 0.42834224356327155 1103 52-53 0.5489500783513606 1103 54-55 0.43183019512770926 1103 56-57 0.5692249803361094 1103 58-59 0.555903734853338 1103 60-61 0.5777614375188662 1103 62-63 0.5927747059685373 1103 64-65 0.628041238377385 1103 66-67 0.6824524701827883 1103 68-69 0.6769563911899965 1103 70-71 0.629183191273583 1103 72-73 0.6319559683231546 1103 74-75 0.471513930596803 1103 76-77 0.44161423663583577 1103 78-79 0.4716308607347237 1103 80-81 0.6346884594509028 1103 82-83 0.5322614745832261 1103 84-85 0.5232111896496576 1103 86-87 0.49647227370472535 1103 88-89 0.35141319798691484 1103 90-91 0.42995994073647736 1103 92-93 0.6048127390359035 1103 94-95 0.4999875698966143 1103 96-97 0.5421048530100521 1103 98-99 0.6879589131214026 1103 100-101 1.1857453841325238 1104 1 0.3398932770671479 1104 2 0.4195936577176944 1104 3 0.45552612721009567 1104 4 0.5481022229313339 1104 5 0.3902661762298152 1104 6 0.3048574366500816 1104 7 0.33589014397330175 1104 8 0.35157846959229033 1104 9 0.5526543813455049 1104 10-11 0.5306596153820564 1104 12-13 0.5644424577788882 1104 14-15 0.5530466540836088 1104 16-17 0.48365707475844033 1104 18-19 0.45640392979694866 1104 20-21 0.5076138588025145 1104 22-23 0.42686402734019424 1104 24-25 0.5537013063818179 1104 26-27 0.1529119651813815 1104 28-29 0.26844982935811146 1104 30-31 0.2871687314622946 1104 32-33 0.3113419518754341 1104 34-35 0.37484492900946265 1104 36-37 0.32510671482044984 1104 38-39 0.18190977313921053 1104 40-41 0.36038968857490516 1104 42-43 0.36177577270067474 1104 44-45 0.5530511078343352 1104 46-47 0.5955496256456385 1104 48-49 0.5697779650750547 1104 50-51 0.4422560312229038 1104 52-53 0.3766263969295309 1104 54-55 0.5026884281094297 1104 56-57 0.6252106167381513 1104 58-59 0.5614417410320911 1104 60-61 0.6087352625829965 1104 62-63 0.48319982356058944 1104 64-65 0.46838586193850773 1104 66-67 0.5143298968255863 1104 68-69 0.4943037131916057 1104 70-71 0.5272577167611203 1104 72-73 0.5802613809806516 1104 74-75 0.4266350592109518 1104 76-77 0.37249321291774606 1104 78-79 0.3805851924046735 1104 80-81 0.5612579561415174 1104 82-83 0.5178758653109163 1104 84-85 0.4809338668076144 1104 86-87 0.5497524133639224 1104 88-89 0.3498068981573432 1104 90-91 0.7327696805668786 1104 92-93 0.47976168646296813 1104 94-95 0.4532357352161718 1104 96-97 0.37442155821232603 1104 98-99 0.36496795166480567 1104 100-101 1.1270385587063103 1105 1 0.44368423079849606 1105 2 0.42465591779684075 1105 3 0.5644687939214883 1105 4 0.5766830698610761 1105 5 0.5347474483188748 1105 6 0.44145610190958706 1105 7 0.5735527396728557 1105 8 0.48256030979328557 1105 9 0.4803461180477768 1105 10-11 0.6127339421414604 1105 12-13 0.5989490218155495 1105 14-15 0.6981626977194182 1105 16-17 0.7912835581498712 1105 18-19 0.7192169826954355 1105 20-21 0.6194887959059443 1105 22-23 0.6340225387407159 1105 24-25 0.48916759683022804 1105 26-27 0.5788920550299395 1105 28-29 0.6568626037484364 1105 30-31 0.41204541117703997 1105 32-33 0.6042875154846215 1105 34-35 0.5594270119374656 1105 36-37 0.5923610182360832 1105 38-39 0.5202012915419942 1105 40-41 0.5813073893257084 1105 42-43 0.4944379727431638 1105 44-45 0.5018001864264505 1105 46-47 0.6497365804882662 1105 48-49 0.7474317488037414 1105 50-51 0.8624378098390153 1105 52-53 0.8473762659756474 1105 54-55 0.8496320418174008 1105 56-57 0.742791831800993 1105 58-59 0.7834879245326789 1105 60-61 0.612447665485746 1105 62-63 0.6840539482424575 1105 64-65 0.8230577977250917 1105 66-67 0.8287322224289824 1105 68-69 0.8787697008763544 1105 70-71 0.8582226985307884 1105 72-73 0.7893103375211723 1105 74-75 0.859446469272509 1105 76-77 0.8491320287677624 1105 78-79 0.9597522998849151 1105 80-81 0.9642757443669332 1105 82-83 0.7008413450924103 1105 84-85 0.8749816363769405 1105 86-87 1.0426977834451954 1105 88-89 0.9194904929253802 1105 90-91 0.89801660497832 1105 92-93 0.9628929336514354 1105 94-95 1.0539493801380395 1105 96-97 1.3141134230730778 1105 98-99 1.432339102932243 1105 100-101 1.884910736531797 1106 1 0.21512856815176917 1106 2 0.034008014218201765 1106 3 0.2477414157903013 1106 4 0.3461881538135074 1106 5 0.24444518919138858 1106 6 0.1794543475596697 1106 7 0.32676376277864705 1106 8 0.36541948926846146 1106 9 0.3197563295434094 1106 10-11 0.4178429949979545 1106 12-13 0.35557975438170075 1106 14-15 0.19778194840477425 1106 16-17 0.17812787656616536 1106 18-19 0.452068524852983 1106 20-21 0.21545649317874904 1106 22-23 -0.03500402929885382 1106 24-25 -0.17103351473446082 1106 26-27 -0.09935288937843012 1106 28-29 0.037797603475993924 1106 30-31 -0.002561785052002108 1106 32-33 0.08589698861688788 1106 34-35 0.17321645689652598 1106 36-37 -0.06414593172295469 1106 38-39 -0.021979926373461467 1106 40-41 0.05653076970074267 1106 42-43 0.2099107093057242 1106 44-45 0.2043467628983393 1106 46-47 0.260771566549046 1106 48-49 0.2201027688903352 1106 50-51 0.06428452217475922 1106 52-53 0.39537334592987605 1106 54-55 0.4486848134490984 1106 56-57 0.409229862784386 1106 58-59 0.24055860679079188 1106 60-61 0.4331528132164806 1106 62-63 0.30448986936102074 1106 64-65 0.17701151647349178 1106 66-67 -0.02654005017770089 1106 68-69 0.09550426969840942 1106 70-71 -0.06983421601075435 1106 72-73 0.06618532133094845 1106 74-75 0.17064661790989888 1106 76-77 0.08682299520555148 1106 78-79 0.24565148907868917 1106 80-81 0.3966012941868122 1106 82-83 0.31416183195131353 1106 84-85 0.4693109950298435 1106 86-87 0.5250617208352715 1106 88-89 0.5498677674838213 1106 90-91 0.5824516846520673 1106 92-93 0.6730585160571998 1106 94-95 0.8047472788484491 1106 96-97 0.7362669367304804 1106 98-99 0.6914851257655883 1106 100-101 1.228287105331166 1107 1 0.22328552620227526 1107 2 0.3000288410115388 1107 3 0.4123677545092086 1107 4 0.3716690207002955 1107 5 0.3273713351897243 1107 6 0.3584719004902652 1107 7 0.45591379703057555 1107 8 0.43415101495875774 1107 9 0.40857781552308836 1107 10-11 0.4841641623288666 1107 12-13 0.42974970249262157 1107 14-15 0.18425975581300236 1107 16-17 0.3174322223948849 1107 18-19 0.5393664643777285 1107 20-21 0.38452916446428986 1107 22-23 0.33684590807484227 1107 24-25 0.31443148502008 1107 26-27 0.2816998045435568 1107 28-29 0.2822201240845743 1107 30-31 0.18307533007546084 1107 32-33 0.40569277866991627 1107 34-35 0.3510939678600664 1107 36-37 0.2880652204884697 1107 38-39 0.36421742508103705 1107 40-41 0.4261860840640779 1107 42-43 0.335290441802897 1107 44-45 0.43357367820262027 1107 46-47 0.5219829362973698 1107 48-49 0.6934494321761022 1107 50-51 0.6346444218825908 1107 52-53 0.6632483031994099 1107 54-55 0.6585341680220793 1107 56-57 0.8109434950515464 1107 58-59 0.8237896647542371 1107 60-61 0.8323437155802509 1107 62-63 0.8107434921610519 1107 64-65 0.9128578813779029 1107 66-67 0.9622610783118475 1107 68-69 0.9373648542196023 1107 70-71 1.0399562729878724 1107 72-73 0.9874089280926981 1107 74-75 0.9199402985756429 1107 76-77 1.0006655480348137 1107 78-79 0.8518206418062562 1107 80-81 0.8644481698300197 1107 82-83 0.9713360681963827 1107 84-85 0.9518285499871872 1107 86-87 0.9737534143037117 1107 88-89 1.0097782620852591 1107 90-91 1.0654053267736963 1107 92-93 1.1123568267007897 1107 94-95 0.9822973584993839 1107 96-97 1.3246262131027748 1107 98-99 1.4607545936289021 1107 100-101 1.819496880709682 1108 1 0.11444036396343904 1108 2 0.15831085270540513 1108 3 0.3113786409269501 1108 4 0.23270452202602598 1108 5 0.1999970475050148 1108 6 0.2170741555041289 1108 7 0.3552644057247747 1108 8 0.2913695683865214 1108 9 -0.00883604690817208 1108 10-11 0.11262034372062146 1108 12-13 0.06640640861035507 1108 14-15 0.5665948003572012 1108 16-17 0.5476024128202397 1108 18-19 0.55357392825006 1108 20-21 0.502820113288891 1108 22-23 0.5819086882877471 1108 24-25 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-5.09824775031969 2316 94-95 -4.2570338066795905 2316 96-97 -5.814946246674175 2316 98-99 -7.342685894322397 2316 100-101 -8.070610477901301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4175.0 3 1607.0 4 1571.0 5 1663.0 6 1839.0 7 2243.0 8 2793.0 9 3614.0 10 4656.0 11 6151.0 12 7911.0 13 9745.0 14 11513.0 15 14567.0 16 18796.0 17 24233.0 18 18362.0 19 12679.0 20 12454.0 21 13197.0 22 14402.0 23 16186.0 24 18711.0 25 21584.0 26 25101.0 27 30179.0 28 35445.0 29 43259.0 30 52651.0 31 65168.0 32 81947.0 33 105553.0 34 140348.0 35 193774.0 36 272508.0 37 364743.0 38 293137.0 39 43229.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82708201017791 18.759597064001866 14.555078103017197 21.858242822803025 2 15.40172999762443 20.195057910171307 40.59216483744421 23.811047254760055 3 19.37770787762653 25.592594601217627 29.8252194925008 25.204478028655046 4 12.416228884908218 16.73624471385118 37.87576790711945 32.97175849412115 5 13.253595941749785 37.99025011513141 33.847578574359325 14.908575368759488 6 30.7205256184766 36.37894994832008 17.95638797007888 14.94413646312444 7 28.261994681759806 33.59746991656834 20.581499116641506 17.559036285030338 8 26.748023311833702 33.58563873690895 19.890715104213502 19.77562284704385 9 26.974515220425605 16.88722123353126 20.728679194849384 35.40958435119374 10-11 24.778752836944903 26.77051109595562 27.09400131375512 21.356734753344362 12-13 26.38581588354631 24.47846529941439 27.66068964685874 21.475029170180566 14-15 23.133566456223154 25.468211074417233 26.707991855517054 24.690230613842555 16-17 21.48692324096548 29.029297335667337 27.261314390048895 22.222465033318286 18-19 21.425381498101867 27.13824555833579 30.299567123923527 21.13680581963882 20-21 22.526641308220622 26.001053072704956 30.17859299543443 21.29371262364 22-23 22.02487360828511 26.124569906619772 30.202849412582328 21.64770707251279 24-25 21.918553949552656 26.404894024817143 30.383059438456705 21.2934925871735 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27.36054912242737 28.592494704238163 22.035917290216787 52-53 22.248835358020873 27.519817804480727 28.28569370035193 21.94565313714646 54-55 21.7543555756389 27.29663101872772 28.515890947431842 22.433122458201538 56-57 22.11692592268086 27.630228227804533 28.07519072959641 22.177655119918196 58-59 22.238889191544313 27.167614675513352 28.21386798404624 22.379628148896096 60-61 21.905255166443897 27.258834847524753 28.875874798277966 21.960035187753377 62-63 21.98115483557239 27.343187299585292 28.727013737092054 21.94864412775026 64-65 21.7663990890176 27.558799134004385 28.45908712976277 22.215714647215236 66-67 21.744712300760458 27.60129798794797 28.18351476980948 22.47047494148209 68-69 21.796836439938 27.480484450574583 28.46350478028657 22.259174329200846 70-71 21.92547213816214 27.488626156150413 28.25141805886733 22.334483646820118 72-73 21.958406381464666 27.362553590251835 28.280662512862413 22.398377515421085 74-75 21.678447571958433 28.06687697541075 28.045183236766064 22.209492215864753 76-77 21.863773182471856 27.666870983707454 28.10653346635285 22.36282236746784 78-79 21.960894007649852 27.64321751757224 28.047530219876105 22.34835825490181 80-81 21.92283936668625 27.965205907312836 27.90690609260682 22.205048633394096 82-83 21.940733465788785 27.855788248018065 27.98754075214412 22.215937534049036 84-85 21.73824739252849 27.81306290191589 27.84328936186771 22.605400343687904 86-87 21.951327890174827 27.717531152806345 27.91570610559105 22.415434851427783 88-89 21.89688886166434 27.62166758340315 27.73365922457399 22.747784330358524 90-91 21.75346039720914 27.556418414512567 28.122554536733695 22.5675666515446 92-93 21.957710547808563 27.56215887655948 28.114534372388107 22.365596203243854 94-95 21.561600286025403 27.36978399708953 28.20167301762576 22.8669426992593 96-97 21.60313335592965 27.241265895881195 28.264125836377556 22.891474911811596 98-99 21.42488560402306 27.57508652361028 27.84554892115108 23.154478951215573 100-101 21.41554168147048 27.375400016822994 28.13218351120926 23.07687479049727 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 277.0 1 219.0 2 278.5 3 378.0 4 331.5 5 337.5 6 556.5 7 1103.5 8 1851.0 9 2331.5 10 2291.5 11 2129.5 12 2194.0 13 2348.0 14 2544.0 15 2813.5 16 3021.5 17 3141.0 18 3371.5 19 3727.0 20 4123.5 21 4652.0 22 5420.5 23 6369.5 24 7454.0 25 9019.5 26 11526.5 27 14514.5 28 17140.0 29 20340.0 30 24652.0 31 29674.5 32 35805.5 33 40942.5 34 45553.5 35 49626.0 36 54392.0 37 59563.0 38 61026.0 39 60404.5 40 60171.0 41 62359.5 42 64521.5 43 66339.0 44 69265.5 45 72279.5 46 72607.0 47 74165.0 48 84174.0 49 83066.0 50 70809.0 51 63943.0 52 60200.5 53 59432.0 54 58396.0 55 55019.0 56 50101.0 57 44682.0 58 40980.5 59 36842.0 60 31088.0 61 25819.5 62 21664.5 63 16826.5 64 12097.0 65 9340.5 66 6935.0 67 5344.0 68 4008.0 69 2719.5 70 2018.0 71 1552.0 72 1285.0 73 1164.0 74 919.0 75 725.0 76 564.5 77 351.0 78 205.5 79 127.5 80 85.5 81 63.5 82 41.5 83 24.5 84 17.5 85 12.5 86 8.0 87 7.5 88 6.5 89 5.0 90 6.0 91 6.0 92 3.5 93 2.5 94 2.0 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0761155751746742 2 0.03047635496769607 3 0.014861616261018182 4 0.021037220315427766 5 0.025355122337210073 6 0.030426146804652086 7 0.07912806495731302 8 0.05678543240274177 9 0.07139600784854006 10-11 0.061781144625617804 12-13 0.040417571250404176 14-15 0.045814948777632064 16-17 0.056961160973395694 18-19 0.053672526294014984 20-21 0.019706703994762282 22-23 0.014058285652314496 24-25 0.023271483570884887 26-27 0.02522960192960012 28-29 0.006652581603327396 30-31 0.001004163260879607 32-33 0.009690175467488207 34-35 0.012125271375121252 36-37 0.004594046918524201 38-39 0.009238302000092383 40-41 0.004493630592436241 42-43 0.0022593673369791156 44-45 7.531224456597052E-4 46-47 5.020816304398035E-4 48-49 0.001029267342401597 50-51 0.0010543714239235873 52-53 7.53122445659705E-5 54-55 4.016653043518427E-4 56-57 0.003991548961996437 58-59 0.0038911326359084763 60-61 0.005322065282661916 62-63 0.0028367612119848894 64-65 6.02497956527764E-4 66-67 5.020816304398035E-4 68-69 0.004568942837002212 70-71 0.0038660285543864865 72-73 0.022141799902395332 74-75 0.01757285706539312 76-77 0.02618355702743575 78-79 0.021463989701301596 80-81 0.013957869326226535 82-83 0.004769775489178132 84-85 0.0037907163098205156 86-87 0.0016819734619733415 88-89 0.0020836387663251844 90-91 0.0069036224185472965 92-93 0.010970483625109705 94-95 0.013882557081660566 96-97 0.01169850198924742 98-99 0.0245266876469844 100-101 0.01935524685345442 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1991708.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.54674709636657 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95873855154514 40.439313715234384 2 13.047870596385561 13.712463127487794 3 4.169587662420211 6.572948051799752 4 1.8714759821689402 3.933599005278222 5 1.0374413314544024 2.7257083635626147 6 0.5612654223321515 1.7695603320733766 7 0.39689254146630504 1.459878840060488 8 0.2932716898975304 1.2328378655655658 9 0.21555137904865673 1.019387142102853 >10 1.2518895385763367 12.535497004315598 >50 0.12147089927574899 4.410697693577868 >100 0.06906302945965023 6.584367301014085 >500 0.003942744118329563 1.4321584367651317 >1k 0.001346302869673509 1.3304894375847123 >5k 1.9232898138192987E-4 0.8410936835775744 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9349 0.4693961162981722 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7296 0.36631875756888055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4157 0.20871533377382628 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3779 0.18973664814320174 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2665 0.1338047545122076 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2176 0.10925296278370122 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 7.531224456597052E-5 16-17 0.0 0.0 0.0 1.0041632608796068E-4 1.0041632608796068E-4 18-19 0.0 0.0 0.0 1.0041632608796068E-4 1.0041632608796068E-4 20-21 0.0 0.0 0.0 1.2552040760995084E-4 1.0041632608796068E-4 22-23 0.0 0.0 0.0 2.0083265217592136E-4 1.5062448913194103E-4 24-25 0.0 0.0 0.0 2.0083265217592136E-4 2.0083265217592136E-4 26-27 2.510408152199017E-5 0.0 0.0 3.2635305978587225E-4 2.0083265217592136E-4 28-29 5.020816304398034E-5 0.0 0.0 7.782265271816954E-4 2.2593673369791155E-4 30-31 5.020816304398034E-5 0.0 0.0 0.0020334306032812038 2.5104081521990174E-4 32-33 5.020816304398034E-5 0.0 0.0 0.003338842842424693 3.0124897826388206E-4 34-35 5.020816304398034E-5 0.0 0.0 0.004368110184826289 3.0124897826388206E-4 36-37 5.020816304398034E-5 0.0 0.0 0.00582414691310172 3.0124897826388206E-4 38-39 5.020816304398034E-5 0.0 0.0 0.007104455070723218 3.0124897826388206E-4 40-41 5.020816304398034E-5 0.0 0.0 0.009188093837048402 3.0124897826388206E-4 42-43 5.020816304398034E-5 0.0 0.0 0.01310433055447887 3.514571413078624E-4 44-45 5.020816304398034E-5 0.0 0.0 0.01930503869041044 4.016653043518427E-4 46-47 5.020816304398034E-5 0.0 0.0 0.03358926107642285 4.769775489178133E-4 48-49 5.020816304398034E-5 0.0 0.0 0.05881886300602297 5.020816304398035E-4 50-51 5.020816304398034E-5 0.0 0.0 0.07862598332687322 5.522897934837837E-4 52-53 5.020816304398034E-5 0.0 0.0 0.09760466895749778 6.276020380497542E-4 54-55 5.020816304398034E-5 0.0 0.0 0.1236124974142796 7.029142826157248E-4 56-57 5.020816304398034E-5 0.0 0.0 0.14575429731667494 7.029142826157248E-4 58-59 5.020816304398034E-5 0.0 0.0 0.17916782982244384 7.029142826157248E-4 60-61 5.020816304398034E-5 0.0 0.0 0.2309073418392656 7.029142826157248E-4 62-63 5.020816304398034E-5 0.0 0.0 0.2674839886168053 7.531224456597052E-4 64-65 5.020816304398034E-5 0.0 0.0 0.3123951904596457 7.531224456597052E-4 66-67 5.020816304398034E-5 0.0 0.0 0.39649386355831273 7.531224456597052E-4 68-69 5.020816304398034E-5 0.0 0.0 0.49332030598862886 7.531224456597052E-4 70-71 5.020816304398034E-5 0.0 0.0 0.5725236831905078 8.033306087036854E-4 72-73 5.020816304398034E-5 0.0 0.0 0.6597603664794236 0.0011547877500115479 74-75 5.020816304398034E-5 0.0 0.0 0.7392147844965226 0.0011547877500115479 76-77 5.020816304398034E-5 0.0 0.0 0.804711333187395 0.0011547877500115479 78-79 5.020816304398034E-5 0.0 0.0 0.8887095899599741 0.0011547877500115479 80-81 5.020816304398034E-5 0.0 0.0 1.0212089322330382 0.001179891831533538 82-83 5.020816304398034E-5 0.0 0.0 1.1691221805606042 0.0012049959130555283 84-85 5.020816304398034E-5 0.0 0.0 1.3520807266928687 0.0012300999945775186 86-87 5.020816304398034E-5 0.0 0.0 1.639422043793568 0.0012552040760995086 88-89 5.020816304398034E-5 0.0 0.0 2.0090796442048733 0.0012552040760995086 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17370 0.0 28.669897 1 GTACATG 12225 0.0 25.110094 1 ATCAACG 20410 0.0 24.26236 3 TCAACGC 20430 0.0 24.239218 4 TATCAAC 20550 0.0 24.093437 2 CAACGCA 20605 0.0 24.033955 5 TACATGG 12535 0.0 23.957176 2 AACGCAG 20920 0.0 23.762342 6 ACATGGG 12350 0.0 23.47314 3 GAGTACT 9095 0.0 23.41257 12-13 GGTATCA 12180 0.0 22.784016 1 AGTACTT 9495 0.0 22.301113 12-13 ACGCAGA 22640 0.0 21.831127 7 CGCAGAG 22735 0.0 21.744274 8 GTACTTT 9640 0.0 21.667116 14-15 TACCTGG 1740 0.0 21.573505 2 GCAGAGT 23000 0.0 20.97865 9 GTACCTG 1840 0.0 20.66031 1 CATGGGG 4755 0.0 20.289192 4 AGAGTAC 17720 0.0 19.810404 10-11 >>END_MODULE