FastQCFastQC Report
Thu 26 May 2016
SRR936680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991708
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111370.5591683118208091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99610.5001235120810882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58030.29135797014421794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50590.2540030968394965No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.1445493014036194No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21350.10719442809889804No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20160.10121965669666437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG10700.027.5204335
GTAAGGT8300.026.3224984
GGTATCA146750.025.3484251
TACCTGG18050.025.0004772
GTACATG117850.024.9533211
ACATGGG120300.023.7684043
GTATCAA196350.023.6874941
TACATGG125050.023.2851312
GAGTACT115700.022.84207512-13
AGGTAAG8550.022.2226452
GTACCTG20750.022.208591
GTACTTT121400.021.67179114-15
GGATACG1100.002875266121.5945741
GGTAAGG9150.021.2829553
AGTACTT118900.020.84934412-13
CATGGGG48000.020.4821934
ATCAACG233600.019.8041083
CATGGGT16800.019.7895584
TATCAAC234450.019.7743152
TCAACGC234300.019.764224
Produced by FastQC (version 0.11.5)