##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936679_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 775223 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19344369297608 33.0 31.0 34.0 31.0 34.0 2 32.29115751209652 33.0 31.0 34.0 31.0 34.0 3 32.31829421985674 34.0 31.0 34.0 31.0 34.0 4 35.91913810606754 37.0 35.0 37.0 35.0 37.0 5 35.95765347519359 37.0 35.0 37.0 35.0 37.0 6 35.99656872925597 37.0 36.0 37.0 35.0 37.0 7 35.97613331905787 37.0 36.0 37.0 35.0 37.0 8 35.963579511959786 37.0 36.0 37.0 35.0 37.0 9 37.594408318638635 39.0 38.0 39.0 35.0 39.0 10-11 37.502821768703974 39.0 37.0 39.0 35.0 39.0 12-13 37.45082189253931 39.0 37.0 39.0 35.0 39.0 14-15 38.644333952939995 40.0 38.0 41.0 35.0 41.0 16-17 38.582705879469515 40.0 38.0 41.0 35.0 41.0 18-19 38.63492440239776 40.0 38.0 41.0 35.0 41.0 20-21 38.55221465307402 40.0 38.0 41.0 35.0 41.0 22-23 38.6464591478839 40.0 38.0 41.0 35.0 41.0 24-25 38.673456540892104 40.0 38.0 41.0 35.0 41.0 26-27 38.57644832519159 40.0 38.0 41.0 35.0 41.0 28-29 38.447238407529184 40.0 38.0 41.0 35.0 41.0 30-31 38.20429153933771 40.0 38.0 41.0 34.0 41.0 32-33 37.935787508884545 40.0 37.0 41.0 34.0 41.0 34-35 37.71542897979033 40.0 37.0 41.0 33.5 41.0 36-37 37.50759523388754 40.0 36.5 41.0 33.0 41.0 38-39 37.28940833798791 39.5 36.0 41.0 33.0 41.0 40-41 37.086084907181544 39.0 36.0 41.0 33.0 41.0 42-43 36.80925359541706 39.0 36.0 41.0 32.0 41.0 44-45 36.42498287589507 39.0 35.0 41.0 31.0 41.0 46-47 35.985105576072954 39.0 35.0 41.0 30.0 41.0 48-49 35.6891455748862 39.0 35.0 41.0 28.5 41.0 50-51 34.723690473579865 38.0 34.0 40.0 26.0 40.5 52-53 34.83979641986886 38.5 35.0 39.5 27.0 40.5 54-55 35.286382756961544 39.0 35.0 40.5 27.0 41.0 56-57 35.166858439442585 39.0 35.0 41.0 26.5 41.0 58-59 34.9607390389604 39.0 35.0 40.5 26.0 41.0 60-61 34.679110397911316 38.5 35.0 40.0 25.5 41.0 62-63 34.35977067243877 38.0 34.5 40.0 23.5 41.0 64-65 34.031347109154396 37.5 34.0 40.0 22.5 41.0 66-67 33.53945509872643 37.0 34.0 39.5 21.5 41.0 68-69 33.150600536877775 36.5 33.5 39.0 20.0 41.0 70-71 32.71806886534584 36.0 33.0 39.0 18.0 40.5 72-73 32.31255909589886 35.5 33.0 38.5 16.5 40.0 74-75 31.88099424294687 35.0 32.5 37.5 11.5 39.5 76-77 31.418136458799598 35.0 32.0 37.0 7.5 39.0 78-79 30.919140685970362 35.0 31.0 36.0 3.5 39.0 80-81 30.40439009162525 35.0 31.0 36.0 2.0 37.5 82-83 29.982183191159187 34.0 30.5 35.5 2.0 37.0 84-85 29.581425602697546 34.0 30.0 35.0 2.0 37.0 86-87 29.293344624707988 34.0 30.0 35.0 2.0 36.0 88-89 28.874216193276 34.0 29.0 35.0 2.0 36.0 90-91 28.448903089820607 34.0 29.0 35.0 2.0 36.0 92-93 28.02817318371617 34.0 29.0 35.0 2.0 35.0 94-95 27.586199712856818 34.0 27.0 35.0 2.0 35.0 96-97 27.08854806939423 34.0 26.5 35.0 2.0 35.0 98-99 26.442405604580877 33.5 25.0 35.0 2.0 35.0 100-101 23.57785759710432 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39897516207042116 1101 2 0.3727434235204612 1101 3 0.3254464675802424 1101 4 0.3569600248741054 1101 5 0.33926134753797044 1101 6 0.36820761902912125 1101 7 0.3465380094209749 1101 8 0.39347825299530825 1101 9 0.29957280457573177 1101 10-11 0.5020318837084474 1101 12-13 0.4518172729865242 1101 14-15 0.4145654687861793 1101 16-17 0.5236112590713589 1101 18-19 0.3765283620440911 1101 20-21 0.3825255284984337 1101 22-23 0.43945382153727763 1101 24-25 0.30566576946738166 1101 26-27 0.28857496071019284 1101 28-29 0.3202345888364633 1101 30-31 0.3688747001468755 1101 32-33 0.28455229082721445 1101 34-35 0.3483518509319268 1101 36-37 0.3393358933332422 1101 38-39 0.2671358684601586 1101 40-41 0.0943307271578675 1101 42-43 0.22964415852467823 1101 44-45 0.23998503252983738 1101 46-47 0.2864588830616128 1101 48-49 0.1842472357232694 1101 50-51 0.06624297281225466 1101 52-53 0.21010370876468443 1101 54-55 0.030496833919194444 1101 56-57 0.08133580390045836 1101 58-59 0.04056940632749928 1101 60-61 0.2914885164349954 1101 62-63 0.29739507603541426 1101 64-65 0.1002476341228018 1101 66-67 0.2774709685946064 1101 68-69 0.3428461488766672 1101 70-71 0.31970304717604847 1101 72-73 0.12523847551337752 1101 74-75 0.21819255152548322 1101 76-77 0.1525841602917133 1101 78-79 0.3219401444069092 1101 80-81 0.3475390556927209 1101 82-83 0.3297097817162715 1101 84-85 0.4034217884793314 1101 86-87 0.4358975180747642 1101 88-89 0.2626427485764147 1101 90-91 0.20686772185142033 1101 92-93 0.13089791343919543 1101 94-95 0.290528755871339 1101 96-97 0.311982833271383 1101 98-99 0.2208551999018944 1101 100-101 0.4898191185430285 1102 1 0.10081997519807118 1102 2 0.0659557428682831 1102 3 0.12920797352913382 1102 4 0.14527828756369132 1102 5 0.15158703185542066 1102 6 0.26656913824506034 1102 7 0.2805225503364568 1102 8 0.3265647927801254 1102 9 0.3194882320767434 1102 10-11 0.4061997178425898 1102 12-13 0.38999974642024426 1102 14-15 0.26119786553832824 1102 16-17 0.22978816034370908 1102 18-19 0.32413143168323444 1102 20-21 0.16281871052139252 1102 22-23 0.09326993031124431 1102 24-25 0.25874248767423325 1102 26-27 0.016891005624117383 1102 28-29 -0.11332916874877697 1102 30-31 0.15125942284075933 1102 32-33 0.24756048570632316 1102 34-35 0.2110631212916516 1102 36-37 0.1214785418524329 1102 38-39 0.02575474866166161 1102 40-41 0.011975013761805542 1102 42-43 -0.12193219483827988 1102 44-45 0.04791476864422606 1102 46-47 0.22630666840468905 1102 48-49 0.18379526492266507 1102 50-51 0.24594666214148475 1102 52-53 0.1684763564621079 1102 54-55 0.3528896356530211 1102 56-57 0.39448028307464966 1102 58-59 0.33927804703893116 1102 60-61 0.21218620489889872 1102 62-63 0.2910689699445541 1102 64-65 0.20952110467440122 1102 66-67 0.17715176731066862 1102 68-69 0.4107175099671778 1102 70-71 0.37587762176409 1102 72-73 0.2417929827958929 1102 74-75 0.14349212450466098 1102 76-77 0.22778309179907907 1102 78-79 0.20144512499642175 1102 80-81 0.41501191021752604 1102 82-83 0.24335464652601502 1102 84-85 0.08840210076986921 1102 86-87 0.3331843071613285 1102 88-89 0.16257205697693422 1102 90-91 -0.02771403436948816 1102 92-93 0.2147100321971216 1102 94-95 -0.042582139842711086 1102 96-97 -0.16301966667663947 1102 98-99 0.0016637177445950613 1102 100-101 0.24869492409558802 1103 1 0.3183882703127239 1103 2 0.2787543470557168 1103 3 0.2672488508970261 1103 4 0.42015565546000744 1103 5 0.41259600493618365 1103 6 0.29705667563289495 1103 7 0.3469143748628838 1103 8 0.4620284019526082 1103 9 0.30877935869688145 1103 10-11 0.4524474745724021 1103 12-13 0.1585620594810635 1103 14-15 0.4214919831853834 1103 16-17 0.46575326502965453 1103 18-19 0.48705865002820303 1103 20-21 0.3765101362442209 1103 22-23 0.25406454646279286 1103 24-25 0.4121786790999522 1103 26-27 0.3208266985453463 1103 28-29 0.46784680333497164 1103 30-31 0.3484054846553093 1103 32-33 0.15524742488877763 1103 34-35 0.5295008082308357 1103 36-37 0.5505667771465497 1103 38-39 0.6224516579338442 1103 40-41 0.48752586121943153 1103 42-43 0.6394147642843535 1103 44-45 0.678103701844627 1103 46-47 0.7526267082850495 1103 48-49 0.6920789139755072 1103 50-51 0.6460428735073904 1103 52-53 0.5796622986355899 1103 54-55 0.6340017991624975 1103 56-57 0.5833576509709602 1103 58-59 0.4693777479362353 1103 60-61 0.4745431341112578 1103 62-63 0.6961661783194302 1103 64-65 0.4324248933085002 1103 66-67 0.46401069054097377 1103 68-69 0.4076023554307895 1103 70-71 0.29492846922172333 1103 72-73 0.258695774421021 1103 74-75 0.27432462699718485 1103 76-77 0.46395208482001493 1103 78-79 0.3495662615866486 1103 80-81 0.31172078359739075 1103 82-83 0.42725993067357493 1103 84-85 0.4806084816272964 1103 86-87 0.5167351546189529 1103 88-89 0.38086866714642653 1103 90-91 0.47274309424466665 1103 92-93 0.7040091348890449 1103 94-95 0.683085856169253 1103 96-97 0.820859695237619 1103 98-99 0.6416193508452928 1103 100-101 0.8647108762391582 1104 1 0.2460821478637456 1104 2 0.29567271440265586 1104 3 0.15163660599906592 1104 4 0.25860463505184583 1104 5 0.2190041682014865 1104 6 0.32242402257167413 1104 7 0.32644498710777725 1104 8 0.26590595297301434 1104 9 0.3506773178805531 1104 10-11 0.3686821684499506 1104 12-13 0.2255212431545246 1104 14-15 0.13271647298130773 1104 16-17 0.22220224462148508 1104 18-19 0.2497525275792256 1104 20-21 -0.001500067837412189 1104 22-23 0.12822781176891596 1104 24-25 0.1099439852224009 1104 26-27 0.11536751487187757 1104 28-29 -0.01977564564462142 1104 30-31 0.2435585458798073 1104 32-33 0.3694310983581701 1104 34-35 0.24135795108797709 1104 36-37 -0.12017812081263202 1104 38-39 -0.1386911992090134 1104 40-41 0.020536065301065776 1104 42-43 0.18639435612109168 1104 44-45 0.1970118651099355 1104 46-47 -0.25792431212311584 1104 48-49 0.08992585275101561 1104 50-51 -0.19241631016608096 1104 52-53 0.08591740067640075 1104 54-55 0.2736650644686236 1104 56-57 0.23790867137912386 1104 58-59 0.34243897242603794 1104 60-61 0.4556247667643163 1104 62-63 0.40152332117656897 1104 64-65 0.3182922402472741 1104 66-67 0.41720456809200357 1104 68-69 0.3327758248185475 1104 70-71 0.24158153044621145 1104 72-73 0.005318223400614386 1104 74-75 0.10391646373187413 1104 76-77 0.17451330930981257 1104 78-79 0.13912748607644687 1104 80-81 0.27565955910759143 1104 82-83 0.2975456449592855 1104 84-85 0.022669706117095956 1104 86-87 0.15654127706793375 1104 88-89 0.12373601408519974 1104 90-91 0.030283910571199613 1104 92-93 0.027254032848230025 1104 94-95 0.06349401943455746 1104 96-97 0.2566147972784343 1104 98-99 0.2718948610493719 1104 100-101 0.30987414154527926 1105 1 0.2944780406676344 1105 2 0.15466040133546954 1105 3 0.3532300617529742 1105 4 0.32394271182743495 1105 5 0.1799112450196887 1105 6 0.31386878419239395 1105 7 0.38686635816142 1105 8 0.3609636837897696 1105 9 0.47122821047140917 1105 10-11 0.35266772509431377 1105 12-13 0.3429587191887862 1105 14-15 0.49388237984509686 1105 16-17 0.595108171083929 1105 18-19 0.4143279610929156 1105 20-21 0.38032537632772545 1105 22-23 0.03473051723523923 1105 24-25 0.23686657054045668 1105 26-27 0.10461062338876559 1105 28-29 0.1289877219153439 1105 30-31 0.15772176858015285 1105 32-33 0.059464543886690535 1105 34-35 0.2581041485231097 1105 36-37 0.1724425600275552 1105 38-39 0.3362491527146432 1105 40-41 0.35932648752422836 1105 42-43 0.27285317764552985 1105 44-45 0.3916872091515202 1105 46-47 0.5564743074499745 1105 48-49 0.702328392054838 1105 50-51 0.5355533119207436 1105 52-53 0.704790691119932 1105 54-55 0.49713019164683914 1105 56-57 0.5470486739354712 1105 58-59 0.6597326539905168 1105 60-61 0.6123468919400636 1105 62-63 0.6057434225782998 1105 64-65 0.39694994550056606 1105 66-67 0.563251817889622 1105 68-69 0.5795825224453992 1105 70-71 0.59013932517162 1105 72-73 0.6974734362164341 1105 74-75 0.8209321426548044 1105 76-77 0.6422245274087395 1105 78-79 0.702819915031327 1105 80-81 0.8514765247247347 1105 82-83 0.5727693252455985 1105 84-85 0.7638673542786556 1105 86-87 0.6142518560803332 1105 88-89 0.6969657443906847 1105 90-91 0.8249549940359024 1105 92-93 0.798725210045621 1105 94-95 0.8614052716181 1105 96-97 1.132586208807556 1105 98-99 1.19407968487452 1105 100-101 1.0662724628779863 1106 1 0.2746406462366977 1106 2 0.22108486447915965 1106 3 0.23103448975658125 1106 4 0.32765512747690195 1106 5 0.33127815910724934 1106 6 0.3764959575758766 1106 7 0.3917306367425013 1106 8 0.42440432592304234 1106 9 0.5420517979788215 1106 10-11 0.44469826654705713 1106 12-13 0.5082642770100989 1106 14-15 0.454781317926674 1106 16-17 0.44222950578994613 1106 18-19 0.4126299277473109 1106 20-21 0.30228363149237225 1106 22-23 0.4512419487859134 1106 24-25 0.4128877604093546 1106 26-27 0.3292453891472462 1106 28-29 0.32396559953348714 1106 30-31 0.36093188381054375 1106 32-33 0.2005589349204584 1106 34-35 0.3024078832044381 1106 36-37 0.1818239049184669 1106 38-39 0.19049495254841986 1106 40-41 0.28013673767138414 1106 42-43 0.3775150810742218 1106 44-45 0.26617603552995206 1106 46-47 0.3107565921287616 1106 48-49 0.5250076362563973 1106 50-51 0.31560095164888935 1106 52-53 0.3555276629439348 1106 54-55 0.5346147875468645 1106 56-57 0.5921686330195399 1106 58-59 0.7623978112942069 1106 60-61 0.7406930813129406 1106 62-63 0.627726368393354 1106 64-65 0.7058647032345817 1106 66-67 0.564374470900006 1106 68-69 0.6085532530020643 1106 70-71 0.6245039708056765 1106 72-73 0.44843019891943925 1106 74-75 0.6713415224565296 1106 76-77 0.5146411027714386 1106 78-79 0.5744770324419832 1106 80-81 0.6301463379796495 1106 82-83 0.3094214935035602 1106 84-85 0.4551945428733397 1106 86-87 0.4682942887266641 1106 88-89 0.31115008214114326 1106 90-91 0.4420498524284078 1106 92-93 0.4766775614993968 1106 94-95 0.5385188023149752 1106 96-97 0.5301321345195582 1106 98-99 0.7475332895992501 1106 100-101 0.986953748467247 1107 1 0.17027715920161768 1107 2 -0.05357898627761415 1107 3 0.10558218423035726 1107 4 0.1417112110155614 1107 5 0.10748489382506676 1107 6 0.14405667563289626 1107 7 0.1699143748628842 1107 8 0.21313951306372303 1107 9 0.21577935869687792 1107 10-11 0.19972525235017713 1107 12-13 0.06200650392550244 1107 14-15 0.0114919831853868 1107 16-17 0.08086437614075948 1107 18-19 0.1389475389170869 1107 20-21 0.1278990251331038 1107 22-23 0.06339787979612055 1107 24-25 0.08390090132217409 1107 26-27 0.14721558743423202 1107 28-29 0.15690235889052673 1107 30-31 0.09812770687754124 1107 32-33 -0.03864146400011492 1107 34-35 -0.014276969546941132 1107 36-37 0.012622332702100891 1107 38-39 0.08122943571162011 1107 40-41 0.1678036389972135 1107 42-43 0.12463698650658017 1107 44-45 0.18993703517796234 1107 46-47 0.03296004161837374 1107 48-49 0.16313446953106592 1107 50-51 -0.12001268204816995 1107 52-53 -0.028504368031079252 1107 54-55 -0.02116486750417579 1107 56-57 -0.22353123791792484 1107 58-59 -0.15173336317486985 1107 60-61 -0.13179019922208113 1107 62-63 0.1919995116527673 1107 64-65 -0.14063066224705523 1107 66-67 -0.15471153168124374 1107 68-69 -0.13145320012476702 1107 70-71 -0.006627086333836019 1107 72-73 -0.10135978113453348 1107 74-75 -0.04178648411392771 1107 76-77 -0.1879923596244275 1107 78-79 -0.21948929396890193 1107 80-81 -0.08961254973594279 1107 82-83 -0.040073402659761115 1107 84-85 -0.14594707392825867 1107 86-87 0.10512404350784621 1107 88-89 -0.00796466618690772 1107 90-91 -0.10564579464422152 1107 92-93 0.14945357933349257 1107 94-95 0.3114191895025833 1107 96-97 0.13108191745984143 1107 98-99 0.35367490640084753 1107 100-101 0.50282198735027 1108 1 0.30612511241798757 1108 2 0.3352234546072026 1108 3 0.32915960604577066 1108 4 0.3440503923021083 1108 5 0.38200561592016413 1108 6 0.34890793421413235 1108 7 0.4165047638788977 1108 8 0.3001748550418526 1108 9 0.36470384382273835 1108 10-11 0.31255388189504885 1108 12-13 0.33585725398906874 1108 14-15 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-2.2485051598468218 2316 48-49 -2.6059925145959184 2316 50-51 -2.5293472363827263 2316 52-53 -2.5499146244413353 2316 54-55 -1.9196630360023406 2316 56-57 -1.9239830083696958 2316 58-59 -2.1485343399758534 2316 60-61 -2.127303019734896 2316 62-63 -2.187487667834418 2316 64-65 -2.092108073724468 2316 66-67 -2.177654144623858 2316 68-69 -2.4355130291845946 2316 70-71 -2.085027574734319 2316 72-73 -2.1038262036009527 2316 74-75 -1.6006753730028187 2316 76-77 -1.8549215415536118 2316 78-79 -2.34992274940236 2316 80-81 -2.2729275680509637 2316 82-83 -2.000531278117638 2316 84-85 -1.9847871227683065 2316 86-87 -1.7958527574689604 2316 88-89 -2.465235728457973 2316 90-91 -2.159943718942145 2316 92-93 -3.2992765793966683 2316 94-95 -2.8670789789955826 2316 96-97 -3.4495468981689754 2316 98-99 -4.624572956847015 2316 100-101 -5.687025387497105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1576.0 3 409.0 4 330.0 5 345.0 6 373.0 7 373.0 8 526.0 9 648.0 10 860.0 11 1280.0 12 2178.0 13 3177.0 14 3724.0 15 5478.0 16 8087.0 17 12907.0 18 8921.0 19 4429.0 20 3753.0 21 3651.0 22 3845.0 23 4248.0 24 4810.0 25 5285.0 26 6238.0 27 7023.0 28 8655.0 29 10864.0 30 13682.0 31 17587.0 32 23703.0 33 33882.0 34 50083.0 35 78505.0 36 117432.0 37 168781.0 38 138669.0 39 18897.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7188926774958 19.40601008087665 14.793757442290401 21.081339799337144 2 14.221081217062615 21.319753078456287 40.353224235465625 24.105941469015473 3 19.992620506991813 27.346698128421238 31.061472982927828 21.59920838165912 4 10.982008657003336 16.98707900321896 40.550255130016325 31.480657209761386 5 11.289331953559069 39.190922392858894 35.104631448014594 14.415114205567441 6 27.25440298969711 37.11828711828712 19.252498296615943 16.37481159539983 7 24.618157143815438 35.7034680619411 22.091544244750953 17.58683054949251 8 24.901493276770992 31.444223390282733 21.476013903188598 22.178269429757673 9 25.67778381370574 17.26914833629897 23.524720996039942 33.52834685395535 10-11 23.443784752645687 26.5967344610751 27.584293513957835 22.375187272321376 12-13 26.348298622782192 25.38565905101196 28.162997763238796 20.103044562967053 14-15 23.314162588985905 25.37337793246264 29.890039706987594 21.42241977156386 16-17 19.202884526058277 29.729030543254986 29.80670475541655 21.261380175270183 18-19 18.544963322170748 28.6278021235789 33.34247241663382 19.48476213761653 20-21 20.46349560734185 26.31213522224624 32.04727229318372 21.177096877228188 22-23 19.22741498933093 26.40936194587286 32.98960959209844 21.373613472697762 24-25 20.322459136299194 26.273060207766008 32.78113103861073 20.62334961732407 26-27 19.752392869121024 25.6473001212559 34.61198111503831 19.98832589458476 28-29 18.638120901985623 27.444232175773937 33.99912025314006 19.918526669100377 30-31 18.624900752635256 26.921561203777816 33.584663242646016 20.868874800940908 32-33 18.88706357821521 26.705907621220298 33.78346913515748 20.623559665407008 34-35 20.29345104569911 26.47399522459989 32.70155813230516 20.530995597395847 36-37 19.732528403135873 25.772342779008607 34.351557133597126 20.14357168425839 38-39 19.84306451175984 26.835181618708425 33.29448429677654 20.027269572755195 40-41 19.013776098253462 27.720019942738798 33.85344408906123 19.412759869946512 42-43 18.713090075051795 27.654547612351944 32.00066305640624 21.631699256190032 44-45 18.80074792009716 27.328946171811356 31.830785335451452 22.039520572640033 46-47 20.09521174325465 27.678418822023954 31.68012414548396 20.546245289237437 48-49 19.945679971053398 27.44354670703368 31.182353923819093 21.42841939809383 50-51 18.94339729734785 28.17319109934643 32.23478024745824 20.64863135584748 52-53 19.47571558121745 29.018367949804503 30.271578374348078 21.234338094629965 54-55 18.765012138708936 27.796353485396143 31.523943313132254 21.914691062762675 56-57 20.245607698459793 27.761280668713397 29.98516550140605 22.00794613142076 58-59 20.711918586276333 27.598713107955223 30.45455307720191 21.234815228566525 60-61 20.448895980846554 27.6276971619413 31.83466995522521 20.088736901986938 62-63 19.851035642831253 28.308129702703365 31.347722385545246 20.49311226892014 64-65 19.07656010350597 29.34769568935826 30.924923344439843 20.65082086269593 66-67 19.425390415863042 28.598244176820813 29.61651841292162 22.359846994394523 68-69 19.045406977569428 28.44016986485911 30.635548002565265 21.8788751550062 70-71 20.43874506008549 28.065360109686267 29.6952334865715 21.800661343656746 72-73 19.8283696992019 27.54102569229747 31.382711446556783 21.247893161943846 74-75 19.136502128521542 29.40346562916697 30.430794181417514 21.029238060893974 76-77 19.0547181520383 28.46270260785647 30.43456103297591 22.04801820712932 78-79 19.288710569327073 28.074684293288648 31.060339648865607 21.57626548851867 80-81 20.206278449445904 28.39971886961836 29.737669728595595 21.65633295234014 82-83 19.534214491033033 27.993586496801314 31.148204186843287 21.323994825322366 84-85 19.707739158424495 28.36048686058265 30.177022033092538 21.75475194790032 86-87 19.190548224889287 29.14510203620858 30.649309598531786 21.015040140370342 88-89 19.37997597726226 28.56647989482634 29.407598255190624 22.645945872720773 90-91 18.81233343075074 28.348916892629887 29.8845635990506 22.95418607756877 92-93 19.942639326379783 28.775873424629612 30.197956251842516 21.08353099714809 94-95 19.456176789308014 28.130902007327517 30.168287837349713 22.244633366014757 96-97 18.388840509595227 28.236736010320918 31.358684083212385 22.015739396871474 98-99 18.943846687422365 28.766433837169913 29.585262376796635 22.70445709861109 100-101 18.786032226274475 27.84235495627245 30.615347977806763 22.756264839646306 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 876.0 1 657.5 2 790.0 3 1011.5 4 707.5 5 610.0 6 1029.0 7 1974.5 8 3054.5 9 3399.0 10 2681.5 11 1970.5 12 1878.5 13 1980.5 14 2030.0 15 2020.0 16 2051.5 17 2101.0 18 2188.5 19 2340.5 20 2549.0 21 2838.5 22 3159.5 23 3551.5 24 4052.5 25 4808.0 26 6017.0 27 7210.0 28 8177.5 29 9551.5 30 11400.0 31 13330.5 32 15832.0 33 18572.0 34 21502.5 35 23483.5 36 25578.0 37 27609.5 38 26933.5 39 25531.0 40 24519.5 41 24100.0 42 23710.0 43 23906.0 44 24662.0 45 24982.5 46 25559.0 47 32789.5 48 51943.5 49 49664.5 50 28813.0 51 20204.5 52 16858.5 53 15172.5 54 14012.0 55 12714.0 56 11077.5 57 9460.0 58 8545.5 59 7679.5 60 6499.5 61 5510.5 62 4390.5 63 3048.0 64 2033.5 65 1570.5 66 1174.0 67 898.0 68 694.0 69 486.0 70 370.0 71 302.0 72 251.0 73 254.0 74 211.0 75 164.0 76 131.0 77 85.0 78 60.0 79 38.0 80 27.5 81 16.5 82 7.0 83 6.5 84 4.5 85 3.5 86 3.0 87 2.0 88 2.0 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0118675529492804 2 0.01006162097873773 3 0.01315750435681088 4 0.016511378016390122 5 0.027991945543411383 6 0.03805356652214911 7 0.004514829926356674 8 0.019220275972204125 9 0.030184862936213194 10-11 0.03637662969235949 12-13 0.02818543825454095 14-15 0.021993671498394655 16-17 0.024638071883832137 18-19 0.021090705513123323 20-21 0.04521279683394327 22-23 0.010964586964009067 24-25 0.003095883378073148 26-27 3.869854222591435E-4 28-29 0.0 30-31 0.0017414344001661455 32-33 0.0016124392594130978 34-35 0.0 36-37 6.449757037652391E-5 38-39 0.0 40-41 4.5148299263566747E-4 42-43 0.0032248785188261956 44-45 8.384684148948109E-4 46-47 4.5148299263566747E-4 48-49 3.224878518826196E-4 50-51 0.0027733955261905283 52-53 0.0018059319705426699 54-55 0.002192917392801813 56-57 3.869854222591435E-4 58-59 0.0028378930965670523 60-61 0.0012899514075304784 62-63 5.159805630121913E-4 64-65 2.5799028150609566E-4 66-67 0.01135157238626821 68-69 0.03276476575127415 70-71 0.03682811268499516 72-73 0.008836167141583777 74-75 0.01992974924634589 76-77 0.018317309986932793 78-79 0.0431488745818945 80-81 0.0636591019616291 82-83 0.03811806409252563 84-85 0.012061045660409973 86-87 0.016833865868272742 88-89 0.01599539745337793 90-91 0.026766491706257424 92-93 0.016446880446013598 94-95 0.008126693867442013 96-97 0.012641523793798688 98-99 0.04327786972264755 100-101 0.06224015541334558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 775223.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.72597176406557 #Duplication Level Percentage of deduplicated Percentage of total 1 79.86358896243505 42.10885336610289 2 11.864395782991929 12.51123594103462 3 3.6105872010572653 5.711151564439257 4 1.5526200529008458 3.2745360427828794 5 0.7540846227827585 1.9879922264279877 6 0.4622987148129052 1.4625089390273422 7 0.316319160534079 1.167476458872694 8 0.21799296796932924 0.9195112859132561 9 0.15238879374136285 0.7231362512370398 >10 1.0487269236178816 10.541696484391643 >50 0.09625559653283886 3.5035432962051076 >100 0.05045440100236818 4.588427326642237 >500 0.0041637127039322154 1.6480681529188093 >1k 0.0048984855340379 5.126677448889187 >5k 9.7969710680758E-4 3.430311420308912 >10k+ 2.44924276701895E-4 1.2948737948062137 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10027 1.2934342763308107 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8078 1.0420227470031205 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6492 0.8374364537687865 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6303 0.8130563721664605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5690 0.7339823508848421 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4856 0.6264004034968003 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3950 0.5095308059745389 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3866 0.49869521415128293 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3493 0.4505800266503961 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2000 0.25799028150609565 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1824 0.23528713673355925 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1733 0.22354857892503188 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1691 0.21813078301340388 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1505 0.194137686833337 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1505 0.194137686833337 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1464 0.18884888606246203 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1432 0.1847210415583645 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1424 0.1836890804323401 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1424 0.1836890804323401 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1382 0.1782712845207121 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1239 0.15982497939302626 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1235 0.15930899883001406 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1233 0.15905100854850798 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1229 0.1585350279854958 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1214 0.15660010087420007 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 967 0.12473830110819725 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 927 0.11957849547807535 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 925 0.11932050519656925 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 925 0.11932050519656925 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 898 0.11583763639623695 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 803 0.10358309802469741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.449757037652391E-5 0.0 18-19 0.0 0.0 0.0 1.9349271112957176E-4 0.0 20-21 0.0 0.0 0.0 3.2248785188261956E-4 0.0 22-23 0.0 0.0 0.0 7.094732741417631E-4 0.0 24-25 0.0 0.0 0.0 0.001676936829789622 0.0 26-27 0.0 0.0 0.0 0.0020639222520487653 0.0 28-29 0.0 0.0 0.0 0.003805356652214911 0.0 30-31 0.0 0.0 0.0 0.008449181719324633 0.0 32-33 0.0 0.0 0.0 0.013544489779070024 0.0 34-35 0.0 0.0 0.0 0.019736256535216316 0.0 36-37 0.0 0.0 0.0 0.030442853217719286 0.0 38-39 0.0 0.0 0.0 0.04695423123410941 0.0 40-41 0.0 0.0 0.0 0.06881890759175102 0.0 42-43 0.0 0.0 0.0 0.09281200377181792 0.0 44-45 0.0 0.0 0.0 0.12344834970066679 0.0 46-47 0.0 0.0 0.0 0.17240200561644842 0.0 48-49 0.0 0.0 0.0 0.21393844093892983 0.0 50-51 0.0 0.0 0.0 0.2592802329136261 0.0 52-53 0.0 0.0 0.0 0.29668882373201 0.0 54-55 0.0 0.0 0.0 0.3434495622549899 0.0 56-57 0.0 0.0 0.0 0.41704129005460366 0.0 58-59 0.0 0.0 0.0 0.4937289012322906 0.0 60-61 0.0 0.0 0.0 0.5782852159959134 0.0 62-63 0.0 0.0 0.0 0.6567142615737664 0.0 64-65 0.0 0.0 0.0 0.7419155520411547 0.0 66-67 0.0 0.0 0.0 0.8433057326730502 0.0 68-69 0.0 0.0 0.0 0.9535320804465296 0.0 70-71 0.0 0.0 0.0 1.0898154466521246 0.0 72-73 0.0 0.0 0.0 1.2243573784575537 0.0 74-75 0.0 0.0 0.0 1.3714763364864044 0.0 76-77 0.0 0.0 0.0 1.5323977745758317 0.0 78-79 0.0 0.0 0.0 1.7249230221497556 0.0 80-81 0.0 0.0 0.0 1.947568635089516 0.0 82-83 0.0 0.0 0.0 2.1685373111994872 0.0 84-85 0.0 0.0 0.0 2.4114351612374763 0.0 86-87 0.0 0.0 0.0 2.6469802882525415 0.0 88-89 0.0 0.0 0.0 2.910323868099889 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTTAT 30 0.003947343 31.67327 64-65 GTACACG 95 3.4890363E-5 30.000448 1 GTACCTG 585 0.0 26.795275 1 GTACATG 6515 0.0 26.247515 1 TACATGG 6400 0.0 26.19961 2 TACCTGG 545 0.0 26.14718 2 GAGTACT 5510 0.0 25.17279 12-13 ACATGGG 6495 0.0 25.08991 3 AGTACTT 5590 0.0 24.005278 12-13 TTGCGCC 70 1.7771308E-5 23.754951 80-81 CATGGGG 1545 0.0 23.678076 4 GTACTTT 6005 0.0 23.100746 14-15 GTGTAGC 330 0.0 23.030647 1 CATGGGA 4380 0.0 22.995663 4 TGCACCG 145 2.3304794E-5 22.934334 5 ATGGGGA 770 0.0 22.827963 5 ATGGGAG 1480 0.0 22.790443 5 GGTAAGG 150 3.0266921E-5 22.171288 3 CATGGGT 695 0.0 21.875065 4 CTATTAC 135 3.7940242E-4 21.111425 1 >>END_MODULE