##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936679_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 775223 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.90880301539041 31.0 31.0 31.0 28.0 34.0 2 31.007889342808458 31.0 31.0 34.0 28.0 34.0 3 31.035580729673914 31.0 31.0 34.0 27.0 34.0 4 34.879362454416345 35.0 35.0 37.0 32.0 37.0 5 34.72057846580919 35.0 35.0 37.0 32.0 37.0 6 34.8993115529338 35.0 35.0 37.0 32.0 37.0 7 34.80660661512881 35.0 35.0 37.0 32.0 37.0 8 34.802209170780536 35.0 35.0 37.0 32.0 37.0 9 36.13036248924503 38.0 35.0 39.0 32.0 39.0 10-11 35.961856781855026 37.0 35.0 39.0 30.0 39.0 12-13 35.79207724745009 37.0 35.0 39.0 30.0 39.0 14-15 36.34495622549899 38.0 35.0 40.0 30.0 41.0 16-17 36.35449412620626 38.0 34.5 40.0 30.5 41.0 18-19 36.680050772487405 38.5 35.0 40.0 31.0 41.0 20-21 36.7310625458739 38.5 35.0 40.0 30.5 41.0 22-23 36.551947633132656 38.0 35.0 40.0 30.0 41.0 24-25 36.312175335355114 38.0 34.5 40.0 30.0 41.0 26-27 35.87669032007564 38.0 34.0 40.0 28.5 41.0 28-29 35.74746685792346 38.0 34.0 40.0 29.0 41.0 30-31 35.49692153096593 38.0 34.0 40.0 27.5 41.0 32-33 35.07691980243104 37.0 33.5 40.0 27.0 41.0 34-35 34.71447764062728 37.0 33.0 40.0 26.0 41.0 36-37 34.33246949587409 37.0 33.0 40.0 25.0 41.0 38-39 33.95595009435995 37.0 33.0 39.0 24.0 40.0 40-41 33.643447111347314 37.0 32.5 39.0 23.5 40.0 42-43 33.1183162780258 36.0 31.5 39.0 21.5 40.0 44-45 32.541537080298184 36.0 31.0 39.0 18.0 40.0 46-47 31.74543324952949 36.0 30.0 39.0 11.5 40.0 48-49 30.991576617308823 35.0 29.0 39.0 7.5 40.0 50-51 30.470676179628313 35.0 28.0 38.0 3.5 40.0 52-53 30.119348239151833 35.0 28.0 38.0 2.0 40.0 54-55 29.653262351607214 34.5 27.0 38.0 2.0 40.0 56-57 29.13222969390743 34.0 26.0 38.0 2.0 40.0 58-59 28.598908314123808 34.0 25.0 38.0 2.0 40.0 60-61 27.923231766859345 33.0 23.0 37.5 2.0 39.0 62-63 27.441512313231158 33.0 23.0 37.0 2.0 39.0 64-65 26.94867089856725 32.0 22.0 36.5 2.0 39.0 66-67 26.27483511196133 31.5 20.5 36.0 2.0 39.0 68-69 25.617392672818013 31.0 18.5 35.5 2.0 38.0 70-71 24.876493602485994 30.5 15.5 35.0 2.0 38.0 72-73 24.05857862834307 29.5 10.5 34.0 2.0 37.0 74-75 23.356906335338348 29.0 8.0 34.0 2.0 36.5 76-77 22.599573929050095 28.5 7.5 32.5 2.0 35.0 78-79 23.162363603763048 29.0 7.0 34.0 2.0 36.0 80-81 22.783475851464676 29.0 2.0 34.0 2.0 35.5 82-83 22.107299448029792 29.0 2.0 34.0 2.0 35.0 84-85 21.37387564610441 28.5 2.0 33.5 2.0 35.0 86-87 20.702239871624037 27.0 2.0 33.0 2.0 35.0 88-89 19.789132288386696 26.0 2.0 32.5 2.0 34.5 90-91 18.78598803183084 25.0 2.0 32.0 2.0 34.0 92-93 17.790715058763737 23.0 2.0 32.0 2.0 34.0 94-95 16.638151860819402 19.0 2.0 31.0 2.0 34.0 96-97 15.337478377189532 4.5 2.0 31.0 2.0 34.0 98-99 13.956161001415076 2.0 2.0 31.0 2.0 34.0 100-101 11.111751070337181 2.0 2.0 24.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32277246550592054 1101 2 0.4319296574933418 1101 3 0.3819258387727942 1101 4 0.38377138165725455 1101 5 0.44518181627199027 1101 6 0.4060303306874289 1101 7 0.4118631568304636 1101 8 0.20378816873952843 1101 9 0.5980595357110303 1101 10-11 0.3137011106381351 1101 12-13 0.4386326783473038 1101 14-15 0.5573742063962115 1101 16-17 0.32633299344166034 1101 18-19 0.7173630548319068 1101 20-21 0.3601774189230369 1101 22-23 0.27085039028051483 1101 24-25 0.49011207873509477 1101 26-27 0.47666051398091014 1101 28-29 0.4765278735471199 1101 30-31 0.39659050909776283 1101 32-33 0.45899408872337233 1101 34-35 0.5055349827047735 1101 36-37 0.6213660825088922 1101 38-39 0.34424105987181264 1101 40-41 0.4832077593727888 1101 42-43 0.7567840624773012 1101 44-45 0.8971411982203179 1101 46-47 0.7259766591610095 1101 48-49 0.9271288797582002 1101 50-51 1.2939780742330846 1101 52-53 0.9277261631044773 1101 54-55 1.1610141514658245 1101 56-57 1.0794054356021014 1101 58-59 0.9830247766230009 1101 60-61 1.055409908322634 1101 62-63 0.9551568297864463 1101 64-65 0.7378167477465176 1101 66-67 0.9808982226541794 1101 68-69 1.0735669641642929 1101 70-71 1.1418521252027496 1101 72-73 1.243275937221707 1101 74-75 1.2928456494229401 1101 76-77 1.219978161612552 1101 78-79 1.2370418785734465 1101 80-81 1.4506242628010142 1101 82-83 1.2988268924675594 1101 84-85 1.2026341331104469 1101 86-87 1.2563763194612214 1101 88-89 0.9258649176840557 1101 90-91 1.0620883475579035 1101 92-93 0.8188642976115581 1101 94-95 0.5790163556855035 1101 96-97 0.7949851201412699 1101 98-99 0.28027225039507364 1101 100-101 0.09078472586021924 1102 1 0.2606341535302299 1102 2 0.49709265905469735 1102 3 0.4092143802981667 1102 4 0.347721636156912 1102 5 0.2471809826259559 1102 6 0.1149601450180242 1102 7 0.24401417151233318 1102 8 0.21751659616826657 1102 9 0.2017351028527017 1102 10-11 0.3453766896607533 1102 12-13 0.4426429910041705 1102 14-15 0.3930013864251478 1102 16-17 0.5049975954647863 1102 18-19 0.3940524503989238 1102 20-21 0.2883888125475238 1102 22-23 0.23031608455815444 1102 24-25 0.18740005591577358 1102 26-27 0.35101659172583055 1102 28-29 0.613460669993934 1102 30-31 0.6488882336005233 1102 32-33 0.6156883554762445 1102 34-35 0.32338926494247033 1102 36-37 0.640957259326008 1102 38-39 0.4184791202685716 1102 40-41 0.6897544549365477 1102 42-43 0.7010570033369419 1102 44-45 0.7560713590783692 1102 46-47 0.4922503306985284 1102 48-49 0.38915442180138626 1102 50-51 0.3421394490955869 1102 52-53 0.5552002453265317 1102 54-55 0.715604600575297 1102 56-57 0.964626633973225 1102 58-59 0.4839163422439121 1102 60-61 0.1728109326577716 1102 62-63 0.4660199702558039 1102 64-65 0.514459015580556 1102 66-67 0.6031486511106188 1102 68-69 0.5324004933681401 1102 70-71 0.470480440851631 1102 72-73 0.42548749519921714 1102 74-75 0.5092871887572699 1102 76-77 0.6784701137600813 1102 78-79 0.5301491506004616 1102 80-81 0.9419952342956641 1102 82-83 0.8950070708994922 1102 84-85 0.6902596379266797 1102 86-87 0.9779048817386418 1102 88-89 0.8578683116728918 1102 90-91 0.8939859021632017 1102 92-93 0.7600204715921031 1102 94-95 0.5235595879549315 1102 96-97 1.0273609816332598 1102 98-99 0.840714062991049 1102 100-101 -2.435794918458356E-4 1103 1 0.3019541934105874 1103 2 0.4743070159839107 1103 3 0.3943240512852064 1103 4 0.43324506586778 1103 5 0.24489085301479463 1103 6 0.34387938729120293 1103 7 0.30099622535082204 1103 8 0.28538499098382175 1103 9 0.3514110749364505 1103 10-11 0.29354967071758153 1103 12-13 0.3145467796382704 1103 14-15 0.2523588141419921 1103 16-17 0.29718403614275246 1103 18-19 0.155497116400916 1103 20-21 0.19517046758242174 1103 22-23 0.07582755016134968 1103 24-25 0.250151800681472 1103 26-27 0.25537401944267657 1103 28-29 0.45912916451037944 1103 30-31 0.6129360900312335 1103 32-33 0.34990670044135186 1103 34-35 0.36557371160248664 1103 36-37 0.4488784956171372 1103 38-39 0.17904741538323066 1103 40-41 0.4161238467610815 1103 42-43 0.6488441419211881 1103 44-45 0.5719033630664043 1103 46-47 0.9168535211272477 1103 48-49 0.7307242124295001 1103 50-51 0.5145133274604916 1103 52-53 0.6086000459247352 1103 54-55 0.21045903726522752 1103 56-57 0.28568994404301407 1103 58-59 0.07396469717910747 1103 60-61 0.0026030562868832874 1103 62-63 0.4287243570952839 1103 64-65 0.5042680883622097 1103 66-67 0.479603287202341 1103 68-69 0.4850595162993443 1103 70-71 0.3038019762454489 1103 72-73 0.7742277743617301 1103 74-75 0.1836296613395234 1103 76-77 0.04112116061950388 1103 78-79 0.5208934177988667 1103 80-81 0.9418531605170024 1103 82-83 0.7386471506602135 1103 84-85 0.4590388004391457 1103 86-87 0.2930893283986613 1103 88-89 0.5140138749829646 1103 90-91 0.3622765302788551 1103 92-93 0.2354536719909035 1103 94-95 0.3673008641264168 1103 96-97 0.32073785102508445 1103 98-99 0.12848774195416013 1103 100-101 0.32463229289100504 1104 1 0.2264031730024243 1104 2 0.28618456700431594 1104 3 0.3056505818974493 1104 4 0.2263062903575772 1104 5 0.31531942444335925 1104 6 0.23065489749528467 1104 7 0.21836357228959713 1104 8 0.13587478690218546 1104 9 0.2845743402425711 1104 10-11 0.34094762990125815 1104 12-13 0.37488351433214717 1104 14-15 0.4100833039379168 1104 16-17 0.3307758728774459 1104 18-19 0.2028032388499028 1104 20-21 0.24943577370486736 1104 22-23 0.2720724481205252 1104 24-25 0.11760078027330678 1104 26-27 0.12115973372839761 1104 28-29 0.30032304206140026 1104 30-31 0.21201772268428698 1104 32-33 0.382192414727065 1104 34-35 0.5699002422147359 1104 36-37 0.4195927813314242 1104 38-39 0.5183331296689389 1104 40-41 0.5400932345161777 1104 42-43 0.5313645500844615 1104 44-45 0.8811176487806804 1104 46-47 0.9384657660251996 1104 48-49 0.9538160491641925 1104 50-51 0.9481255723584496 1104 52-53 0.8396204540879992 1104 54-55 0.4341937311427806 1104 56-57 0.7770266787368918 1104 58-59 0.8400769420770651 1104 60-61 0.5727153011848394 1104 62-63 0.3199998672993658 1104 64-65 0.28201298632139427 1104 66-67 0.3388481851615275 1104 68-69 0.5577942101768976 1104 70-71 0.10973054767402246 1104 72-73 0.2537277743617281 1104 74-75 0.4027827225640124 1104 76-77 0.22148850755828065 1104 78-79 0.4051893361662131 1104 80-81 0.43986336459863296 1104 82-83 0.26379000780306683 1104 84-85 0.06268165758200439 1104 86-87 0.16064034880682243 1104 88-89 0.47754448722786336 1104 90-91 0.6408173466053881 1104 92-93 0.6464026515827399 1104 94-95 0.8761273947386634 1104 96-97 0.8614317285761057 1104 98-99 0.2897938644031388 1104 100-101 0.29470372146243307 1105 1 0.18526943349409564 1105 2 0.3814510660882924 1105 3 0.39944931224554026 1105 4 0.3131198049074513 1105 5 0.31257561293128333 1105 6 0.23373742486949567 1105 7 0.30531981616501724 1105 8 0.05679208910490985 1105 9 0.07975136721202603 1105 10-11 0.35226052666747876 1105 12-13 0.3665018944608178 1105 14-15 0.4977627807390732 1105 16-17 0.42388758520328906 1105 18-19 0.4171944024134504 1105 20-21 0.17954207509807674 1105 22-23 0.23936825997345323 1105 24-25 0.25084073596330825 1105 26-27 0.5784293430334913 1105 28-29 0.3410467010448244 1105 30-31 0.7806396390917669 1105 32-33 0.6744307087920873 1105 34-35 0.221893127051338 1105 36-37 0.49932734739166307 1105 38-39 0.24366119408886533 1105 40-41 0.4173545356963544 1105 42-43 0.5040915323178581 1105 44-45 0.4736914632751663 1105 46-47 0.7501290952399806 1105 48-49 0.772293105957683 1105 50-51 1.042147982992848 1105 52-53 1.2483077703506211 1105 54-55 1.0191709370564617 1105 56-57 1.2015260609532454 1105 58-59 1.3249657410204456 1105 60-61 0.8845873986668416 1105 62-63 0.9332076556339075 1105 64-65 0.8423672532891437 1105 66-67 1.2652776087054747 1105 68-69 1.077165988115631 1105 70-71 0.8121537507757175 1105 72-73 0.8253874820861533 1105 74-75 0.8815597239700068 1105 76-77 1.0237370270078117 1105 78-79 0.8617399731224573 1105 80-81 1.1501047262790074 1105 82-83 1.3512713677792085 1105 84-85 1.4284511177669117 1105 86-87 1.5178492448913552 1105 88-89 1.8851996787407934 1105 90-91 1.8280771565836602 1105 92-93 1.5966572210514478 1105 94-95 1.623370801495934 1105 96-97 1.4414038217975236 1105 98-99 0.8867528776535334 1105 100-101 0.9320508732667871 1106 1 0.05363740354786373 1106 2 0.26231757564600144 1106 3 0.3603377788459241 1106 4 0.15318170789523577 1106 5 0.15064903675290964 1106 6 0.10597653618243896 1106 7 0.3059624344321321 1106 8 0.18218752530272297 1106 9 0.21822733681607076 1106 10-11 0.43519961791927386 1106 12-13 0.29431921892021506 1106 14-15 0.14767085215677866 1106 16-17 0.32581444796957726 1106 18-19 0.39032182601021503 1106 20-21 0.145225377825291 1106 22-23 0.21716651531446018 1106 24-25 0.18092265601410418 1106 26-27 0.12152555059368098 1106 28-29 0.40118882660119226 1106 30-31 0.20526977535329394 1106 32-33 0.2979225399344898 1106 34-35 0.5383941973469462 1106 36-37 0.7187781788272716 1106 38-39 0.49462291696718097 1106 40-41 0.4673630231074313 1106 42-43 0.588448682575887 1106 44-45 0.3803558445869939 1106 46-47 0.8184659815285151 1106 48-49 0.7367110128518846 1106 50-51 0.7977667593506688 1106 52-53 0.6065514714791149 1106 54-55 0.4659912442346048 1106 56-57 0.5748150760387887 1106 58-59 0.2986674426912508 1106 60-61 0.19405659377368423 1106 62-63 0.6076763106327725 1106 64-65 0.1951376765353885 1106 66-67 0.27472894718122376 1106 68-69 -0.130075647375417 1106 70-71 0.07607916737533316 1106 72-73 0.12276156528041682 1106 74-75 0.1512014670417443 1106 76-77 -0.05931284149242799 1106 78-79 -0.06517680395403502 1106 80-81 -0.004839025333051694 1106 82-83 0.15947186026950533 1106 84-85 0.2995989905130614 1106 86-87 0.2599636684197826 1106 88-89 0.3276506226070417 1106 90-91 0.38035519976143206 1106 92-93 0.3987952770595413 1106 94-95 0.2639719306522892 1106 96-97 0.39487407066605584 1106 98-99 0.17081825937760264 1106 100-101 -0.20173993519769517 1107 1 -0.021601362144966885 1107 2 0.15008479376168538 1107 3 0.07487960684076 1107 4 0.21768951031222628 1107 5 0.14055751968145813 1107 6 0.21299049840231277 1107 7 -0.018892663538068177 1107 8 0.04249610209492971 1107 9 0.07974440826978224 1107 10-11 -0.12728366261575275 1107 12-13 0.23026900186048493 1107 14-15 0.2771921474753256 1107 16-17 0.3222951472538611 1107 18-19 0.4574971164009227 1107 20-21 0.13672602313797455 1107 22-23 0.16538310571689863 1107 24-25 0.29959624512591887 1107 26-27 0.03687401944267776 1107 28-29 0.27951805339926494 1107 30-31 -0.10150835441321959 1107 32-33 -0.07542663289198259 1107 34-35 -0.29142628839750984 1107 36-37 0.10443405117269577 1107 38-39 0.25904741538322895 1107 40-41 0.11884606898330219 1107 42-43 0.17312191969897128 1107 44-45 0.021514474177514842 1107 46-47 -0.22547981220608904 1107 48-49 -0.13410912090383675 1107 50-51 -0.351375561428398 1107 52-53 -0.449177731853041 1107 54-55 0.07301459282078682 1107 56-57 0.16352327737634909 1107 58-59 -0.01497974726533613 1107 60-61 -0.12956361037978326 1107 62-63 -0.42322008734915784 1107 64-65 -0.47578746719334575 1107 66-67 -0.6345078239087698 1107 68-69 -0.4289404837006572 1107 70-71 -0.28392024597677334 1107 72-73 -0.10849444786049389 1107 74-75 -0.10987033866047824 1107 76-77 -0.1629899504916068 1107 78-79 0.08039341779886655 1107 80-81 0.14268649385033427 1107 82-83 -0.0927972937842334 1107 84-85 -0.08946119956085496 1107 86-87 0.28975599506532745 1107 88-89 0.24323609720518746 1107 90-91 0.16933208583441228 1107 92-93 0.2372870053242373 1107 94-95 0.6703564196819691 1107 96-97 0.4417378510250831 1107 98-99 0.49670996417638236 1107 100-101 -0.06331215155343983 1108 1 -0.04741880430108125 1108 2 0.06599122651022427 1108 3 0.048477369363009615 1108 4 0.01763865854512403 1108 5 0.061282157362619216 1108 6 -0.08809772941359739 1108 7 0.01887494388628852 1108 8 0.09319277130418868 1108 9 0.014168512007387335 1108 10-11 0.14564921305168355 1108 12-13 -0.15591203043037893 1108 14-15 -0.1963929021051456 1108 16-17 0.24529158763016312 1108 18-19 0.07725684180137904 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-2.170722775088823 2316 74-75 -2.1103648441549794 2316 76-77 -1.8938568613585183 2316 78-79 -1.791199988794542 2316 80-81 -2.1589984878346478 2316 82-83 -1.9414297724167113 2316 84-85 -2.167263397363051 2316 86-87 -2.6037238584145257 2316 88-89 -2.164645465676376 2316 90-91 -2.403256436754109 2316 92-93 -2.702694679657448 2316 94-95 -2.616572762247209 2316 96-97 -1.9674929182056857 2316 98-99 -1.6131496206832026 2316 100-101 -1.3687578169991053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 18.0 4 30.0 5 93.0 6 209.0 7 512.0 8 1132.0 9 1972.0 10 3143.0 11 4560.0 12 6573.0 13 8643.0 14 11457.0 15 14933.0 16 18247.0 17 21580.0 18 18344.0 19 14075.0 20 14449.0 21 14907.0 22 16045.0 23 17599.0 24 19440.0 25 22135.0 26 25286.0 27 29259.0 28 33601.0 29 37892.0 30 42471.0 31 47240.0 32 52684.0 33 58066.0 34 63100.0 35 63787.0 36 54145.0 37 31121.0 38 6396.0 39 76.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.141013815372744 23.914179301839255 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19.55403109604998 44-45 21.257916643285196 27.03052749241634 31.689083852724476 20.02247201157399 46-47 21.83345953357937 29.191213366992464 30.499288591798745 18.476038507629415 48-49 21.749778286598836 29.079703952142154 30.312333714949126 18.858184046309884 50-51 20.031178386940937 29.793483497835766 30.779466123334576 19.395871991888715 52-53 21.296898874317368 30.900578353070777 28.73080299296263 19.07171977964922 54-55 20.351873852746508 29.48604333473938 29.138852017682698 21.02323079483141 56-57 21.581559757596086 29.482392174443095 29.218250602300838 19.717797465659984 58-59 23.41041972362417 28.468258703258787 28.608929809568345 19.512391763548703 60-61 20.897093472875188 30.01740833213472 30.269019683218662 18.816478511771425 62-63 20.891566840101415 29.139424382384604 30.346114874561337 19.622893902952647 64-65 20.624219966912086 30.938426163317562 29.06499227626152 19.372361593508835 66-67 21.493277022037127 29.17618285003699 28.79454654163461 20.53599358629128 68-69 20.94292976819874 29.76187788751844 28.546209647302707 20.748982696980114 70-71 21.930124993791914 30.168015030988837 28.57816417343429 19.32369580178496 72-73 22.043180668930145 28.38721994613059 29.644882405754323 19.924716979184943 74-75 20.4121123304742 31.876010253142184 28.056882492643613 19.654994923739995 76-77 21.373259512857096 29.492243432136156 28.285995866947705 20.84850118805904 78-79 21.124988147936968 29.94608359461623 28.71991914151718 20.209009115929625 80-81 21.659311641922738 29.840739312209514 28.676921132033122 19.823027913834625 82-83 21.392424168152303 29.47818762077497 29.018823480847573 20.110564730225157 84-85 20.099714915958256 30.36280491737723 29.21414842429793 20.323331742366584 86-87 20.836628101220427 30.284667945445094 27.62090454439326 21.257799408941217 88-89 20.395146325527456 30.420926553118427 27.687332343059555 21.496594778294558 90-91 21.3692392040507 28.752668752217243 28.211823136711068 21.666268907020996 92-93 21.571579222337245 30.57516858389528 27.9263939009878 19.926858292779677 94-95 21.108717525347643 30.031603931787103 28.05448775831377 20.805190784551485 96-97 19.651931738219336 30.062246725613264 28.820730591192323 21.46509094497508 98-99 20.88830439418048 30.57828212304015 27.162664461696927 21.370749021082442 100-101 20.67695117845335 30.075553623465094 26.990587078872718 22.256908119208834 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1474.0 1 998.0 2 689.5 3 856.5 4 724.5 5 650.0 6 1020.0 7 1712.5 8 2364.5 9 2677.0 10 2344.5 11 1906.5 12 1870.5 13 1942.0 14 2066.5 15 2180.5 16 2269.0 17 2394.0 18 2595.0 19 2853.5 20 3069.5 21 3327.0 22 3684.0 23 4204.5 24 4792.5 25 5504.0 26 6700.5 27 7909.0 28 8896.5 29 10237.0 30 12100.0 31 14123.5 32 16730.5 33 19491.5 34 21725.0 35 23411.5 36 25675.0 37 27663.5 38 27097.0 39 25964.5 40 25069.0 41 24280.0 42 23754.5 43 23627.5 44 23997.5 45 24311.5 46 25302.0 47 32514.0 48 46103.5 49 45706.0 50 31819.0 51 22224.0 52 17363.5 53 15010.0 54 13432.5 55 11882.5 56 10334.5 57 8864.0 58 7874.5 59 7084.0 60 6119.0 61 5097.0 62 4138.0 63 3110.0 64 2200.5 65 1648.0 66 1180.5 67 857.0 68 680.5 69 515.5 70 408.5 71 332.5 72 260.5 73 235.5 74 209.0 75 145.0 76 98.5 77 84.5 78 64.0 79 42.5 80 27.5 81 18.0 82 15.0 83 11.0 84 5.5 85 4.0 86 2.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009287650134219444 2 0.007094732741417631 3 0.0036118639410853397 4 1.2899514075304783E-4 5 0.003482868800332291 6 0.0 7 3.869854222591435E-4 8 0.0016769368297896217 9 0.0 10-11 0.0 12-13 0.0 14-15 3.224878518826196E-4 16-17 0.0026444003854374804 18-19 0.00148344411866005 20-21 7.73970844518287E-4 22-23 1.9349271112957176E-4 24-25 4.5148299263566747E-4 26-27 1.2899514075304783E-4 28-29 0.0 30-31 5.159805630121913E-4 32-33 0.003998849363344483 34-35 9.674635556478588E-4 36-37 7.73970844518287E-4 38-39 0.005482293482004534 40-41 6.449757037652391E-5 42-43 0.001547941689036574 44-45 0.004708322637486246 46-47 0.0 48-49 0.0013544489779070022 50-51 8.384684148948109E-4 52-53 5.804781333887153E-4 54-55 2.5799028150609566E-4 56-57 0.009094157423089872 58-59 0.0013544489779070022 60-61 0.0025154052446844325 62-63 4.5148299263566747E-4 64-65 0.0019994246816722413 66-67 0.0010964586964009066 68-69 0.00148344411866005 70-71 0.003160380948449672 72-73 0.0019349271112957176 74-75 0.0059337764746402 76-77 0.0011609562667774305 78-79 0.005353298341251485 80-81 0.0020639222520487653 82-83 0.0027088979558140044 84-85 0.0016769368297896217 86-87 5.159805630121913E-4 88-89 0.002128419822425289 90-91 0.006191766756146296 92-93 0.0019994246816722413 94-95 3.869854222591435E-4 96-97 1.9349271112957176E-4 98-99 5.804781333887153E-4 100-101 0.001547941689036574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 775223.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.22432359804771 #Duplication Level Percentage of deduplicated Percentage of total 1 81.67926125141967 44.290026933464596 2 10.785983989390198 11.697253723281111 3 3.310259383716971 5.38489728048429 4 1.3541204503351056 2.9370506195881942 5 0.6903927746732964 1.871804061181943 6 0.4042895769193542 1.3153397307715715 7 0.2786015146178916 1.057488507848274 8 0.20862199233403456 0.9049909137592093 9 0.16120327760791012 0.7867024821069513 >10 0.9777122621156706 10.069507396600361 >50 0.09302389278878624 3.4258851308845437 >100 0.04698876756977926 4.784037352056963 >500 0.0045319115928578605 1.640766115519171 >1k 0.004293389930075868 5.922718171840565 >5k 4.770433255639853E-4 1.7861406318240385 >10k+ 2.3852166278199265E-4 2.125390948788155 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16433 2.119777147994835 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7251 0.9353437656003499 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6559 0.8460791281992407 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4838 0.6240784909632454 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4736 0.6109209866064346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4272 0.5510672412970203 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3997 0.5155935775899322 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3294 0.4249099936405396 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3030 0.3908552764817349 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2603 0.3357743513801835 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2549 0.3288086137795189 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2367 0.30533149816246424 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2178 0.28095141656013817 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1743 0.22483853033256235 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1709 0.22045269554695876 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1500 0.19349271112957175 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1477 0.19052582289225165 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1408 0.18162515818029135 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1389 0.17917425050598346 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1368 0.17646535255016943 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1335 0.17220851290531886 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 856 0.11041984048460894 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 846 0.10912988907707848 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 846 0.10912988907707848 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.10900089393632542 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 789 0.10177716605415474 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.449757037652391E-5 0.0 18-19 0.0 0.0 0.0 1.9349271112957176E-4 0.0 20-21 0.0 0.0 0.0 3.2248785188261956E-4 0.0 22-23 0.0 0.0 0.0 7.094732741417631E-4 0.0 24-25 0.0 0.0 0.0 0.001676936829789622 0.0 26-27 0.0 0.0 0.0 0.0020639222520487653 0.0 28-29 0.0 0.0 0.0 0.003934351792967959 0.0 30-31 0.0 0.0 0.0 0.008707172000830729 0.0 32-33 0.0 0.0 0.0 0.014898938756977025 0.0 34-35 0.0 0.0 0.0 0.021606686076135515 0.0 36-37 0.0 0.0 0.0 0.03192629733637934 0.0 38-39 0.0 0.0 0.0 0.048050689930510315 0.0 40-41 0.0 0.0 0.0 0.06997986385852845 0.0 42-43 0.0 0.0 0.0 0.09416645274972492 0.0 44-45 0.0 0.0 0.0 0.12364184241179635 0.0 46-47 0.0 0.0 0.0 0.1705315760755292 0.0 48-49 0.0 0.0 0.0 0.20910112316069054 0.0 50-51 0.0 0.0 0.0 0.2512825341869372 0.0 52-53 0.0 0.0 0.0 0.28533725134574184 0.0 54-55 0.0 0.0 0.0 0.3283571307868832 0.0 56-57 0.0 0.0 0.0 0.3976920189416465 0.0 58-59 0.0 0.0 0.0 0.46934881962996455 0.0 60-61 0.0 0.0 0.0 0.5452624599631333 0.0 62-63 0.0 0.0 0.0 0.6136298845622485 0.0 64-65 0.0 0.0 0.0 0.6907689787325711 0.0 66-67 0.0 0.0 0.0 0.7819685432449759 0.0 68-69 0.0 0.0 0.0 0.880456333209928 0.0 70-71 0.0 0.0 0.0 1.0016472679474164 0.0 72-73 0.0 0.0 0.0 1.1227092075441518 0.0 74-75 0.0 0.0 0.0 1.2545422413937666 0.0 76-77 0.0 0.0 0.0 1.3990812966075568 0.0 78-79 0.0 0.0 0.0 1.5716122973647582 0.0 80-81 0.0 0.0 0.0 1.7678139064501441 0.0 82-83 0.0 0.0 0.0 1.9624030762761167 0.0 84-85 0.0 0.0 0.0 2.1700852528885237 0.0 86-87 0.0 0.0 0.0 2.365383895988638 0.0 88-89 0.0 0.0 0.0 2.5862880745282326 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 205 0.0 35.912 94-95 TACCTGG 505 0.0 25.397455 2 GTACCTG 585 0.0 22.737776 1 GAGTACT 7240 0.0 22.404844 12-13 ATCCCGA 110 0.0028788317 21.587934 6 AGTACTT 7220 0.0 21.51297 12-13 GTACTTT 7540 0.0 21.355913 14-15 GGACCGG 115 0.0037251748 20.649326 6 CGGGTCG 105 8.6124055E-7 20.356962 14-15 GGTACTC 215 1.0779568E-6 19.883547 3 GTACTCC 220 1.3445388E-6 19.430393 4 ACTTTTT 8580 0.0 19.042892 16-17 TACTTTT 8380 0.0 18.47836 14-15 GGCTTCG 130 0.0075669754 18.266712 7 GTGTAGC 315 6.7848305E-9 18.097414 1 CATCCCG 135 0.009401691 17.591303 5 GGTGTGC 405 2.7284841E-11 17.590168 8 GGTAAGG 225 3.5354635E-5 16.88874 3 TGTATGG 310 1.1143493E-7 16.855776 2 CGAGTCG 100 2.760618E-4 16.62539 60-61 >>END_MODULE