##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936678_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 749890 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13084719092134 33.0 31.0 34.0 31.0 34.0 2 32.22558375228367 33.0 31.0 34.0 31.0 34.0 3 32.25810052140981 34.0 31.0 34.0 31.0 34.0 4 35.8811745722706 37.0 35.0 37.0 35.0 37.0 5 35.85621224446252 37.0 35.0 37.0 35.0 37.0 6 36.00011735054475 37.0 36.0 37.0 35.0 37.0 7 35.91598234407713 37.0 36.0 37.0 35.0 37.0 8 35.92696928882903 37.0 36.0 37.0 35.0 37.0 9 37.56065822987372 39.0 37.0 39.0 35.0 39.0 10-11 37.46652242328875 39.0 37.0 39.0 35.0 39.0 12-13 37.409120004267294 39.0 37.0 39.0 35.0 39.0 14-15 38.58357625785115 40.0 38.0 41.0 34.5 41.0 16-17 38.55810652228993 40.0 38.0 41.0 35.0 41.0 18-19 38.606791662777205 40.0 38.0 41.0 35.0 41.0 20-21 38.55838389630479 40.0 38.0 41.0 35.0 41.0 22-23 38.45746576164504 40.0 38.0 41.0 34.0 41.0 24-25 38.32788475643095 40.0 38.0 41.0 34.0 41.0 26-27 38.14582672125245 40.0 38.0 41.0 34.0 41.0 28-29 38.23188934377042 40.0 38.0 41.0 34.0 41.0 30-31 38.11063089253091 40.0 37.5 41.0 34.0 41.0 32-33 37.87093640400592 40.0 37.0 41.0 34.0 41.0 34-35 37.675950472736005 40.0 37.0 41.0 33.0 41.0 36-37 37.442168851431546 40.0 36.0 41.0 33.0 41.0 38-39 37.23885036472016 39.5 36.0 41.0 33.0 41.0 40-41 37.08534251690248 39.0 36.0 41.0 33.0 41.0 42-43 36.78322020563016 39.0 36.0 41.0 31.5 41.0 44-45 36.40175959140674 39.0 35.0 41.0 31.0 41.0 46-47 35.880425129018924 39.0 35.0 40.0 29.5 41.0 48-49 35.481826001146835 39.0 35.0 40.0 27.5 41.0 50-51 34.753573857499106 38.0 34.5 40.0 26.0 40.5 52-53 34.93132526103828 38.5 35.0 40.0 27.0 40.5 54-55 35.3136693381696 39.0 35.0 40.5 27.0 41.0 56-57 35.20527077304671 39.0 35.0 41.0 26.5 41.0 58-59 34.99817839949859 39.0 35.0 40.5 26.0 41.0 60-61 34.691376068490044 38.5 35.0 40.0 25.5 41.0 62-63 34.41353198469109 38.0 35.0 40.0 24.0 41.0 64-65 34.13025177025964 38.0 34.0 40.0 23.0 41.0 66-67 33.708076517889296 37.0 34.0 40.0 22.0 41.0 68-69 33.3252030297777 36.5 34.0 39.0 21.0 41.0 70-71 32.88702543039646 36.0 33.0 39.0 19.5 40.5 72-73 32.46046286788729 35.5 33.0 38.5 18.0 40.0 74-75 32.02817079838376 35.0 33.0 37.5 14.5 39.5 76-77 31.60719172145248 35.0 32.0 37.0 9.0 39.0 78-79 31.12724199549267 35.0 32.0 36.5 6.5 39.0 80-81 30.65973676139167 35.0 31.0 36.0 2.0 38.5 82-83 30.24462854552001 35.0 31.0 35.5 2.0 37.0 84-85 29.822693995119284 34.0 30.5 35.0 2.0 37.0 86-87 29.554577338009572 34.0 30.0 35.0 2.0 36.0 88-89 29.163411300324046 34.0 30.0 35.0 2.0 36.0 90-91 28.79047060235501 34.0 29.0 35.0 2.0 36.0 92-93 28.45422728666871 34.0 29.0 35.0 2.0 35.0 94-95 28.097885689901183 34.0 29.0 35.0 2.0 35.0 96-97 27.6156689647815 34.0 28.5 35.0 2.0 35.0 98-99 27.057345744042458 34.0 27.0 35.0 2.0 35.0 100-101 25.102586379335634 32.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.320948329095593 1101 2 0.381581049884673 1101 3 0.43202488680668694 1101 4 0.41292526565966625 1101 5 0.2862921715595377 1101 6 0.340344661544961 1101 7 0.21881467403635924 1101 8 0.3304708308505653 1101 9 0.45156233217964825 1101 10-11 0.40031754136558817 1101 12-13 0.48722906078833006 1101 14-15 0.5080881162849167 1101 16-17 0.47641841813322117 1101 18-19 0.5035428008318306 1101 20-21 0.5458898448467338 1101 22-23 0.5203120795035474 1101 24-25 0.47776610877679104 1101 26-27 0.5053039181966383 1101 28-29 0.6051380863360478 1101 30-31 0.38292427993808786 1101 32-33 0.26647760562136114 1101 34-35 0.35351430719718024 1101 36-37 0.6031101974679345 1101 38-39 0.7357840032794982 1101 40-41 0.805306689766148 1101 42-43 0.6189048121813627 1101 44-45 0.7656780547195225 1101 46-47 0.9343626402691072 1101 48-49 1.0817234917332144 1101 50-51 1.062922535308438 1101 52-53 0.9233601966100977 1101 54-55 1.0708204062767166 1101 56-57 0.9146076723841645 1101 58-59 1.0320242825097168 1101 60-61 1.111480367310179 1101 62-63 1.2159165772174134 1101 64-65 1.2933040671241827 1101 66-67 1.0172996003562602 1101 68-69 0.8828188082425399 1101 70-71 0.9236563512019487 1101 72-73 0.9557865167090966 1101 74-75 0.7347015464261766 1101 76-77 0.7874933331521774 1101 78-79 0.7755762186824207 1101 80-81 0.6704287760236909 1101 82-83 0.8529775968806916 1101 84-85 0.7330955163182473 1101 86-87 0.7293346487036523 1101 88-89 0.6858871567732123 1101 90-91 0.8217606266543136 1101 92-93 0.5207545210320141 1101 94-95 0.4712817337248367 1101 96-97 0.6449452699473319 1101 98-99 0.41799950745750536 1101 100-101 0.3916950098747414 1102 1 0.10422681802847933 1102 2 0.19931771688617061 1102 3 0.3284663949207882 1102 4 0.2462245103439571 1102 5 0.27739442903493483 1102 6 0.24933644033100677 1102 7 0.22319093042495552 1102 8 0.09169497239147972 1102 9 0.3095203777261162 1102 10-11 0.27752942067483133 1102 12-13 0.38854181668011734 1102 14-15 0.41787442281408715 1102 16-17 0.2546798244170745 1102 18-19 0.1898150799827718 1102 20-21 0.26780816037530286 1102 22-23 0.0374237744920336 1102 24-25 0.20800306321216055 1102 26-27 0.2714803941242039 1102 28-29 0.3188829927052197 1102 30-31 0.30281736663904013 1102 32-33 0.44801055401517687 1102 34-35 0.28688652982953755 1102 36-37 0.3350772012693284 1102 38-39 0.27595697190866275 1102 40-41 0.20802393834018318 1102 42-43 0.33592767497435005 1102 44-45 0.3871445847088637 1102 46-47 0.43720248695489516 1102 48-49 0.6695705454452323 1102 50-51 0.5636589711525914 1102 52-53 0.48864520393814104 1102 54-55 0.505941437117464 1102 56-57 0.6230510878489639 1102 58-59 0.5268553137498415 1102 60-61 0.10787201321100781 1102 62-63 0.3930660077352641 1102 64-65 0.5964088370742076 1102 66-67 0.5880591640376949 1102 68-69 0.7006178030106369 1102 70-71 0.7475235289159556 1102 72-73 0.4284996625427624 1102 74-75 0.45878015444745657 1102 76-77 0.5012836238174145 1102 78-79 0.4557488484421768 1102 80-81 0.6665420649086542 1102 82-83 0.6695439541676542 1102 84-85 0.7233953794392143 1102 86-87 0.6263703885342444 1102 88-89 0.2626011400984183 1102 90-91 0.18054466638917432 1102 92-93 0.40377801073341146 1102 94-95 0.753469184180311 1102 96-97 0.42574154044741874 1102 98-99 0.10413926446393518 1102 100-101 0.5971291439484254 1103 1 0.30427277358255367 1103 2 0.32555381414050544 1103 3 0.34332456369911313 1103 4 0.28034234228949373 1103 5 0.43879009683104186 1103 6 0.2976040588094264 1103 7 0.23286064571482257 1103 8 0.26000659390820147 1103 9 0.4301406110279373 1103 10-11 0.512329561636939 1103 12-13 0.4981940307615673 1103 14-15 0.3889801658296719 1103 16-17 0.6129265843123974 1103 18-19 0.47233637311967414 1103 20-21 0.48149553402728884 1103 22-23 0.46142686041939385 1103 24-25 0.4405339998720734 1103 26-27 0.45481626731544367 1103 28-29 0.5862455010688947 1103 30-31 0.4788676177278077 1103 32-33 0.5268213528560537 1103 34-35 0.517975104993063 1103 36-37 0.7031180898068712 1103 38-39 0.6460235955564926 1103 40-41 0.5008411804830857 1103 42-43 0.5339312276006041 1103 44-45 0.5229557478606068 1103 46-47 0.6622114756707873 1103 48-49 0.7445298878760198 1103 50-51 0.20054818493164106 1103 52-53 0.4717928498470272 1103 54-55 0.7593857225562175 1103 56-57 0.5394393622774345 1103 58-59 0.6102146409414786 1103 60-61 0.8539401941092137 1103 62-63 0.7517752995673206 1103 64-65 0.6798080479721023 1103 66-67 0.6609897949622123 1103 68-69 0.681279562989296 1103 70-71 0.8053388304486759 1103 72-73 0.796248864273359 1103 74-75 0.683463525442118 1103 76-77 0.6702060017911293 1103 78-79 0.9571269197851962 1103 80-81 0.805566545170386 1103 82-83 0.7918006099843673 1103 84-85 0.7408090818139037 1103 86-87 0.7548209244045765 1103 88-89 0.6688851454225961 1103 90-91 0.9751727478519427 1103 92-93 0.9146037582254998 1103 94-95 1.0073694534846211 1103 96-97 0.9490616030231678 1103 98-99 1.0060146320488386 1103 100-101 1.1877246850691208 1104 1 0.21146677615345766 1104 2 0.29356066318043617 1104 3 0.35815243386863216 1104 4 0.19219861287678697 1104 5 0.2644609412112118 1104 6 0.06345376154904159 1104 7 0.19467373320578218 1104 8 0.1564449927191589 1104 9 0.45335905723987935 1104 10-11 0.35083231330401077 1104 12-13 0.29593143736556726 1104 14-15 0.4179379468210769 1104 16-17 0.1837870326131963 1104 18-19 0.28237845153214636 1104 20-21 0.3262121324044074 1104 22-23 0.33590339090866905 1104 24-25 0.3170102592116777 1104 26-27 0.24389445884753513 1104 28-29 0.1807951415445146 1104 30-31 0.35906088719033136 1104 32-33 0.38954178749894197 1104 34-35 0.4683898635694206 1104 36-37 0.31553975767864273 1104 38-39 0.6155016826457498 1104 40-41 0.49001653398192246 1104 42-43 0.2935469984690826 1104 44-45 0.26243488942082394 1104 46-47 0.36875346370003115 1104 48-49 0.3822927524216908 1104 50-51 0.5263633914070951 1104 52-53 0.5344974674255596 1104 54-55 0.6956768469235328 1104 56-57 0.7643316556011257 1104 58-59 0.8050231289305572 1104 60-61 0.690140684187142 1104 62-63 0.8412132665473138 1104 64-65 0.7768043121321426 1104 66-67 0.7114939325053982 1104 68-69 0.6967250518621171 1104 70-71 0.8690125570494658 1104 72-73 0.6642378877328241 1104 74-75 0.681220264013664 1104 76-77 0.5841348099376731 1104 78-79 0.6918370909107665 1104 80-81 0.886503467722001 1104 82-83 0.7825626912087564 1104 84-85 0.8415976754509238 1104 86-87 0.6901040247500418 1104 88-89 0.6916403776070581 1104 90-91 0.6957053058980556 1104 92-93 0.8075950412655892 1104 94-95 1.0722282045893046 1104 96-97 1.1616893948605593 1104 98-99 1.0920004619676966 1104 100-101 0.9611477488595916 1105 1 0.0036732143476356782 1105 2 0.038723835189387046 1105 3 0.039280754321353584 1105 4 0.24015777500355995 1105 5 0.21080052480849787 1105 6 0.1474298057232062 1105 7 0.2052448088424228 1105 8 0.006566672266437479 1105 9 0.1898766466651054 1105 10-11 0.19216455917587894 1105 12-13 0.022326660981427437 1105 14-15 0.09547130971646567 1105 16-17 0.10900558069376842 1105 18-19 0.01841697723002511 1105 20-21 -0.20183751073930267 1105 22-23 -0.17742527056352486 1105 24-25 -0.14976409781353084 1105 26-27 -0.02536970982512088 1105 28-29 0.07872751305513503 1105 30-31 0.1344413667291562 1105 32-33 -0.015248585827634997 1105 34-35 -0.11486164620716721 1105 36-37 -0.17273307901209733 1105 38-39 -0.2488018669129346 1105 40-41 -0.30651269550411797 1105 42-43 -0.32842552993161434 1105 44-45 -0.12191609140607085 1105 46-47 -0.049848594030443394 1105 48-49 0.026176177608569162 1105 50-51 0.13443713290799764 1105 52-53 0.12198070679377793 1105 54-55 0.17034783704136203 1105 56-57 0.22339942134291846 1105 58-59 0.1275966645528399 1105 60-61 0.37476894129260074 1105 62-63 0.05671078016698061 1105 64-65 0.03764461366515093 1105 66-67 -0.03682208590755209 1105 68-69 0.13041101833029956 1105 70-71 0.2955805339488293 1105 72-73 -0.003520859469333004 1105 74-75 0.08587932372902785 1105 76-77 0.36534719749626987 1105 78-79 0.23705912309118204 1105 80-81 0.4832063743058974 1105 82-83 0.42231666624993025 1105 84-85 0.22599880619968715 1105 86-87 0.16320387060502028 1105 88-89 0.07853448736675972 1105 90-91 -0.022471929204776586 1105 92-93 0.020460343854381335 1105 94-95 0.05275749742261837 1105 96-97 0.09786372126792386 1105 98-99 -0.2471086906569937 1105 100-101 -0.12127958654308557 1106 1 0.05009463273344039 1106 2 0.05550412259729853 1106 3 0.03688568839975659 1106 4 0.15782115030783217 1106 5 0.3118407678463626 1106 6 0.24442795382642402 1106 7 0.4211808254043632 1106 8 0.2973778138381533 1106 9 0.29772469947786107 1106 10-11 0.3649920847776116 1106 12-13 0.3398144160889771 1106 14-15 0.31623384910633945 1106 16-17 0.13000452670302565 1106 18-19 -0.03659810481143211 1106 20-21 0.15279802680944954 1106 22-23 0.17104671242167058 1106 24-25 0.09833413628023635 1106 26-27 0.05282373386723549 1106 28-29 0.012195358250579602 1106 30-31 0.16546602903429886 1106 32-33 -0.009665265298536951 1106 34-35 -0.02013456511458145 1106 36-37 0.04943455673301855 1106 38-39 0.2321043225196675 1106 40-41 0.12049574989996614 1106 42-43 0.12978616316569713 1106 44-45 0.21455023615052937 1106 46-47 0.07375001610349585 1106 48-49 0.10602945454935053 1106 50-51 0.3036363425497228 1106 52-53 0.2887700245571594 1106 54-55 0.2319874261197512 1106 56-57 0.2569808872796102 1106 58-59 0.1609382195530955 1106 60-61 0.4454105817442695 1106 62-63 0.33622656441966114 1106 64-65 0.492968614732618 1106 66-67 0.34437415315156983 1106 68-69 0.3962916397265772 1106 70-71 0.36762120559162526 1106 72-73 0.3017846765429155 1106 74-75 0.2024106698450332 1106 76-77 0.10530059007951564 1106 78-79 0.07141746916136071 1106 80-81 0.03788989404193899 1106 82-83 0.07832953518039076 1106 84-85 0.027435441408368177 1106 86-87 0.3879714400376706 1106 88-89 0.24319425861572697 1106 90-91 0.35042851314786105 1106 92-93 0.29779401683690665 1106 94-95 0.3689061990731517 1106 96-97 0.25261975341059895 1106 98-99 0.6913528063333381 1106 100-101 1.1435625439738537 1107 1 0.23825060725106795 1107 2 0.21051470435144637 1107 3 0.26799066047792763 1107 4 0.14329749079798404 1107 5 0.22680169882531942 1107 6 0.21572797482838268 1107 7 0.2394250619833116 1107 8 0.3906130303467634 1107 9 0.42838647645864114 1107 10-11 0.27643922716547564 1107 12-13 0.38478552493833007 1107 14-15 0.5738214784164555 1107 16-17 0.5452145193254196 1107 18-19 0.5867924184603481 1107 20-21 0.6219547713987552 1107 22-23 0.545632731581911 1107 24-25 0.5210092468097542 1107 26-27 0.5532491580798009 1107 28-29 0.4578070267212624 1107 30-31 0.5494517692455005 1107 32-33 0.39847676176705704 1107 34-35 0.1460083989613068 1107 36-37 0.3988572722319219 1107 38-39 0.41592931699992164 1107 40-41 0.2980780479446352 1107 42-43 0.24176158549347093 1107 44-45 0.4968784374677284 1107 46-47 0.7351810031415198 1107 48-49 0.5484571728215926 1107 50-51 0.21270730223135814 1107 52-53 0.24048303015229777 1107 54-55 0.3794871164755449 1107 56-57 0.3608301686358004 1107 58-59 0.47210372034201953 1107 60-61 0.2284590424086872 1107 62-63 0.528954502943165 1107 64-65 0.2738778378218427 1107 66-67 0.30591095773107213 1107 68-69 0.3594897527817196 1107 70-71 0.39625117807367616 1107 72-73 0.2665471659746217 1107 74-75 0.16211249593807153 1107 76-77 0.2510110754400756 1107 78-79 0.4764644004526559 1107 80-81 0.4757200690043817 1107 82-83 0.6676511181892657 1107 84-85 0.5199030095435582 1107 86-87 0.5588371513846475 1107 88-89 0.5293647406375115 1107 90-91 0.8622744234210451 1107 92-93 0.6037278768346432 1107 94-95 0.6551911148229976 1107 96-97 0.8927081770254119 1107 98-99 0.8688462499538581 1107 100-101 1.013886243333566 1108 1 0.12221234314902318 1108 2 0.05900914737892293 1108 3 0.02418453740073545 1108 4 -0.042553996875824396 1108 5 0.21349926524953844 1108 6 0.19172935857464068 1108 7 0.3712342222662741 1108 8 0.2996551671793384 1108 9 0.3338109376917586 1108 10-11 0.20527157399327223 1108 12-13 0.2682877222218494 1108 14-15 0.49168133306446293 1108 16-17 0.5418951407213015 1108 18-19 0.313315638719331 1108 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-2.7931744727712022 2316 62-63 -2.822538497630255 2316 64-65 -2.7759125213294915 2316 66-67 -3.498272126856513 2316 68-69 -3.1149035513369228 2316 70-71 -2.5130535087276265 2316 72-73 -2.516588976949407 2316 74-75 -2.7273539062882755 2316 76-77 -3.3277725867777477 2316 78-79 -3.1216893396768626 2316 80-81 -3.1775294956686366 2316 82-83 -2.8518636303206257 2316 84-85 -2.6729928749724827 2316 86-87 -2.7952559743945997 2316 88-89 -2.7703322402186217 2316 90-91 -2.970370628581456 2316 92-93 -3.1500914269578324 2316 94-95 -3.0301569100287793 2316 96-97 -3.1476417679997617 2316 98-99 -3.5125738366380688 2316 100-101 -4.083094349763279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1477.0 3 360.0 4 328.0 5 312.0 6 326.0 7 387.0 8 489.0 9 631.0 10 838.0 11 1260.0 12 2084.0 13 3029.0 14 3531.0 15 5180.0 16 7514.0 17 11579.0 18 9458.0 19 4191.0 20 3644.0 21 3546.0 22 3619.0 23 3980.0 24 4379.0 25 4954.0 26 5733.0 27 6817.0 28 8171.0 29 10029.0 30 12840.0 31 16748.0 32 22551.0 33 31605.0 34 47469.0 35 74522.0 36 111497.0 37 162635.0 38 140330.0 39 21839.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56429686947778 19.447434086723188 14.803330439270695 21.18493860452834 2 14.244715609788624 21.228912449156496 40.33139961325599 24.194972327798894 3 19.777814823458804 27.333844563731535 31.0265728670023 21.861767745807356 4 10.912450142070087 16.99838587036271 40.40793458105999 31.68122940650721 5 11.254305847948535 39.09683255020932 35.2165664748213 14.432295127020847 6 27.281569460417927 37.06988336317521 19.241873980995482 16.40667319541138 7 24.580254488222582 35.676634566649774 22.029103422519807 17.71400752260784 8 24.874756582281858 31.628564569050603 21.371808893749833 22.124869954917706 9 25.540002027174815 17.26966226200701 23.542034536657187 33.64830117416099 10-11 23.4005413460012 26.631808679945518 27.610446990647176 22.357202983406104 12-13 26.229246860557183 25.425434772893567 28.24381299479533 20.10150537175392 14-15 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33.24993898787893 20.081056103145826 40-41 18.989361524792546 27.824263458121212 33.73606846497238 19.45030655211386 42-43 18.695537663982932 27.749562434365156 31.931189678451766 21.623710223200145 44-45 18.77730014675652 27.510079925961634 31.726079387209843 21.986540540071996 46-47 20.02409738169346 27.764264396418596 31.648450272979193 20.56318794890875 48-49 19.84234937043764 27.517649349367773 31.159602926311464 21.480398353883125 50-51 18.864647280937056 28.176785265405268 32.20982248528601 20.748744968371668 52-53 19.36701045225541 29.111824997399605 30.250264706902115 21.27089984344287 54-55 18.668024219923627 27.931391552098106 31.470431543243336 21.930152684734928 56-57 20.191910833673944 27.835938974195184 29.900137288724387 22.07201290340648 58-59 20.679766174518303 27.833086959101877 30.281560072305336 21.20558679407448 60-61 20.431785919317015 27.743768882049324 31.742779397654708 20.08166580097895 62-63 19.761349437178865 28.449571466482105 31.26747772652534 20.52160136981369 64-65 19.115392745004296 29.45019099990065 30.79142437450783 20.64299188058722 66-67 19.406301050175028 28.82140356726121 29.446774462410403 22.32552092015336 68-69 18.917758327987826 28.574743515711788 30.596278460654187 21.911219695646203 70-71 20.3560447386478 28.271928719035127 29.63371949190574 21.738307050411333 72-73 19.667795972771298 27.67851248982324 31.40035512798543 21.253336409420026 74-75 19.165617042386597 29.490852766107263 30.346732740209486 20.996797451296658 76-77 18.935710985148788 28.5877647036859 30.408143261878866 22.068381049286444 78-79 19.231320780138105 28.194198121356152 31.024569908215515 21.549911190290228 80-81 20.12890051092033 28.57125708009131 29.685865026599735 21.613977382388622 82-83 19.553039845145562 28.20352449872677 30.959685436921685 21.283750219205977 84-85 19.700800842852097 28.62796481892683 29.943587589269637 21.727646748951436 86-87 19.195453957449534 29.3813071872762 30.479409579923157 20.94382927535111 88-89 19.282461275846664 28.869114695507797 29.280260850431084 22.568163178214455 90-91 18.723802080763274 28.593290932102462 29.67955018597772 23.003356801156542 92-93 19.894197959046224 28.951122071121965 30.14844694527115 21.006233024560665 94-95 19.403136348057554 28.231133302127216 30.111635213835246 22.254095135979984 96-97 18.40625873074402 28.36825853204146 31.271716423433833 21.95376631378069 98-99 18.965452850296575 29.051563965112727 29.43016743383276 22.55281575075794 100-101 18.573351547929377 27.943573885178672 30.756274830505536 22.726799736386415 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 958.0 1 721.0 2 759.0 3 954.5 4 709.0 5 622.5 6 1056.0 7 1943.5 8 2945.5 9 3248.5 10 2615.0 11 1991.0 12 1934.5 13 2004.5 14 1994.0 15 2007.0 16 1976.5 17 2074.0 18 2194.0 19 2255.0 20 2435.5 21 2652.0 22 3010.5 23 3515.0 24 3946.0 25 4614.5 26 5841.0 27 7163.5 28 7957.5 29 9090.5 30 10891.0 31 12952.5 32 15622.5 33 18213.0 34 20847.0 35 22652.5 36 24708.5 37 26659.0 38 25963.0 39 24755.5 40 23760.5 41 23345.5 42 23023.5 43 23229.0 44 23967.5 45 24149.0 46 24654.5 47 31453.0 48 49635.0 49 47649.0 50 27821.5 51 19474.0 52 16319.5 53 14776.0 54 13553.0 55 12160.5 56 10677.5 57 9223.0 58 8316.5 59 7378.0 60 6214.0 61 5236.0 62 4261.0 63 3054.0 64 1976.0 65 1532.5 66 1178.0 67 861.0 68 636.0 69 460.5 70 353.0 71 286.0 72 253.0 73 258.0 74 211.5 75 161.5 76 136.5 77 93.0 78 61.5 79 40.5 80 26.5 81 19.5 82 13.5 83 9.0 84 5.0 85 3.5 86 2.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010534878448839163 2 0.00533411567029831 3 0.008667937964234755 4 0.03467175185693902 5 0.04373974849644614 6 0.051207510434863775 7 0.020002933763618665 8 0.020002933763618665 9 0.010401525557081705 10-11 0.0369387510168158 12-13 0.036605368787422154 14-15 0.013735347851018148 16-17 0.017535905266105694 18-19 0.024336902745736043 20-21 0.021269786235314513 22-23 0.009601408206536958 24-25 0.0014002053634533064 26-27 0.0014668818093320354 28-29 4.0005867527237324E-4 30-31 0.0038005574150875463 32-33 0.0012001760258171198 34-35 8.001173505447465E-4 36-37 0.0044006454279961055 38-39 0.005200762778540853 40-41 0.0023336756057555108 42-43 3.333822293936444E-4 44-45 0.001133499579938391 46-47 0.002133646268119324 48-49 5.33411567029831E-4 50-51 0.001133499579938391 52-53 5.33411567029831E-4 54-55 0.0015335582552107642 56-57 0.0014002053634533064 58-59 0.0019336169304831374 60-61 6.000880129085599E-4 62-63 9.334702423022043E-4 64-65 0.002733734281027884 66-67 0.0020002933763618664 68-69 0.005200762778540853 70-71 0.009134673085385858 72-73 0.0026003813892704264 74-75 0.0024670284975129683 76-77 0.0024670284975129683 78-79 0.03380495806051554 80-81 0.0219365506941018 82-83 0.003267145848057715 84-85 0.0073344090466601775 86-87 0.0068009974796303455 88-89 0.0046673512115110215 90-91 0.009201349531264585 92-93 0.012468495379322302 94-95 0.004934056995025937 96-97 0.0034004987398151727 98-99 0.0214698155729507 100-101 0.04107269066129699 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 749890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.94428341647066 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87374589274174 42.28858240080478 2 11.91537452999391 12.617019322587868 3 3.551290062422142 5.64061522676921 4 1.5272487043349683 3.2343635299899267 5 0.8056201301935781 2.1326490249491394 6 0.45966565648561386 1.460200127627543 7 0.2921646342318939 1.0827913039330015 8 0.2169661913411846 0.9189695620927905 9 0.1651485908827077 0.7869306421372355 >10 1.0388635505307786 10.554940516678741 >50 0.09244809270599486 3.3744699917607384 >100 0.05138790444136854 4.644921201861128 >500 0.003778522385324141 1.4757107240726974 >1k 0.005038029847098854 5.016082787446743 >5k 0.0012595074617747135 4.77175363728848 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9928 1.3239275093680407 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7936 1.0582885489871847 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6284 0.8379895718038646 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6130 0.8174532264732162 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5501 0.7335742575577752 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4315 0.5754177279334303 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3959 0.5279440984677752 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3726 0.4968728746882876 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3598 0.47980370454333304 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1981 0.2641720785715238 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1912 0.2549707290402592 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1738 0.23176732587446158 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1663 0.22176585899265225 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1404 0.1872274600274707 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1352 0.1802931096560829 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1334 0.17789275760444864 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1306 0.17415887663523982 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1290 0.17202523036712053 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1289 0.17189187747536305 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1236 0.1648241742122178 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1221 0.16282388083585592 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1155 0.1540225899798637 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1081 0.14415447598981182 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1026 0.13682006694315166 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1025 0.1366867140513942 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 966 0.1288188934377042 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 928 0.12375148355092079 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 897 0.1196175439064396 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 797 0.10628225473069385 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 796 0.10614890183893637 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 761 0.10148155062742535 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.667644587872889E-5 0.0 14-15 0.0 0.0 0.0 1.3335289175745777E-4 0.0 16-17 0.0 0.0 0.0 1.3335289175745777E-4 0.0 18-19 0.0 0.0 0.0 1.3335289175745777E-4 0.0 20-21 0.0 0.0 0.0 1.3335289175745777E-4 0.0 22-23 0.0 0.0 0.0 3.3338222939364445E-4 0.0 24-25 0.0 0.0 0.0 0.0010001466881809332 0.0 26-27 0.0 0.0 0.0 0.0014668818093320354 0.0 28-29 0.0 0.0 0.0 0.0037338809692088176 0.0 30-31 0.0 0.0 0.0 0.0061342330208430575 0.0 32-33 0.0 0.0 0.0 0.009801437544173145 0.0 34-35 0.0 0.0 0.0 0.014802170985077811 0.0 36-37 0.0 0.0 0.0 0.022269932923495446 0.0 38-39 0.0 0.0 0.0 0.033671605168758084 0.0 40-41 0.0 0.0 0.0 0.05480803851231514 0.0 42-43 0.0 0.0 0.0 0.07361079625011668 0.0 44-45 0.0 0.0 0.0 0.09881449279227619 0.0 46-47 0.0 0.0 0.0 0.1348197735667898 0.0 48-49 0.0 0.0 0.0 0.1659576737921562 0.0 50-51 0.0 0.0 0.0 0.203629865713638 0.0 52-53 0.0 0.0 0.0 0.2394351171505154 0.0 54-55 0.0 0.0 0.0 0.28004107269066125 0.0 56-57 0.0 0.0 0.0 0.34798437104108604 0.0 58-59 0.0 0.0 0.0 0.4124604942058169 0.0 60-61 0.0 0.0 0.0 0.4826041152702396 0.0 62-63 0.0 0.0 0.0 0.5557481763992052 0.0 64-65 0.0 0.0 0.0 0.6332262065102883 0.0 66-67 0.0 0.0 0.0 0.7222392617583913 0.0 68-69 0.0 0.0 0.0 0.8324554267959301 0.0 70-71 0.0 0.0 0.0 0.9582738801690915 0.0 72-73 0.0 0.0 0.0 1.081825334382376 0.0 74-75 0.0 0.0 0.0 1.2277133979650348 0.0 76-77 0.0 0.0 0.0 1.3794689887850218 0.0 78-79 0.0 0.0 0.0 1.569296830201763 0.0 80-81 0.0 0.0 0.0 1.7853951912947232 0.0 82-83 0.0 0.0 0.0 1.9902252330341783 0.0 84-85 0.0 0.0 0.0 2.2176585899265224 0.0 86-87 0.0 0.0 0.0 2.4407579778367494 0.0 88-89 0.0 0.0 0.0 2.6963287948899173 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6275 0.0 27.722307 1 TACATGG 6365 0.0 26.654703 2 ACATGGG 6365 0.0 26.132061 3 ATGGGTA 320 0.0 25.24829 5 TACCTGG 585 0.0 25.18314 2 GTGTAGC 305 0.0 24.933437 1 GTACCTG 620 0.0 24.531286 1 CATGGGG 1785 0.0 24.227423 4 GAGTACT 5400 0.0 23.231153 12-13 CATGGGA 4175 0.0 23.107 4 GTACTAT 105 0.002187642 22.63303 1 CGTAGCC 105 0.0021893398 22.63001 3 AGTACTT 5545 0.0 21.938099 12-13 GTACTTT 5815 0.0 21.488626 14-15 GTATCAA 12255 0.0 21.486143 1 ATGGGGA 895 0.0 21.240757 5 GATTGGC 225 7.10188E-8 21.122753 9 GTTGTGC 205 6.7866677E-7 20.865158 8 GGTAAGG 140 4.8294003E-4 20.369728 1 GTATAGT 210 8.5486136E-7 20.369728 1 >>END_MODULE