FastQCFastQC Report
Thu 26 May 2016
SRR936678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749890
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT158422.112576511221646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72720.9697422288602329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65460.8729280294443186No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC48590.6479617010494872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45460.606222245929403No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC45200.6027550707437092No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38050.5074077531371268No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33150.4420648361759725No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29340.3912573844163811No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25440.33924975663097257No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25180.33578258144527867No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22630.30177759404712695No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC21710.28950912800544076No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.2371014415447599No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14620.19496192774940324No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG14320.19096134099667952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13720.18296016749123203No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13190.1758924642280868No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT12930.1724252890423929No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11900.15868994119137475No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11720.1562895891397405No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.11948419101468215No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG8780.11708383896304791No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.11308325221032417No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA8020.10694901918948112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCC650.005840928329.232683
GTAAGCG1300.029.2287894-95
GTAGGAC1351.3412395E-524.6312393
GGACTAT1252.2579657E-422.7954066
CTCCCGC1302.944196E-421.9186617
GAGTACT71900.021.76369512-13
AGTACTT73250.021.10325612-13
CGCAATT804.9932052E-520.77983550-51
GTGTAGC3501.8189894E-1220.558671
GTACTTT76300.020.47752614-15
TACCTGG5050.019.7537632
GTGTAAC2051.4589352E-518.7200891
ACTTTTT86200.018.53889816-17
TACTTTT83300.018.5286814-15
GGTACTC2101.9532275E-518.0964183
GATATAC1600.00111434917.9888361
ATCGCAA800.001191788617.81128748-49
TAGGACA3751.70985E-1017.7344914
GTGGTAC2453.4211316E-617.6217171
GTGATCC1350.00940835117.5890489
Produced by FastQC (version 0.11.5)