##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936677_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 761782 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13027611573915 33.0 31.0 34.0 31.0 34.0 2 32.19891254978458 33.0 31.0 34.0 31.0 34.0 3 32.25113483910095 34.0 31.0 34.0 31.0 34.0 4 35.85071188345222 37.0 35.0 37.0 35.0 37.0 5 35.85421025962808 37.0 35.0 37.0 35.0 37.0 6 36.01142846641165 37.0 36.0 37.0 35.0 37.0 7 35.93677325008992 37.0 36.0 37.0 35.0 37.0 8 35.91473807467228 37.0 36.0 37.0 35.0 37.0 9 37.526808194470334 39.0 37.0 39.0 35.0 39.0 10-11 37.466348640424684 39.0 37.0 39.0 35.0 39.0 12-13 37.40495049764893 39.0 37.0 39.0 35.0 39.0 14-15 38.557337926073345 40.0 38.0 41.0 34.5 41.0 16-17 38.48379063826659 40.0 38.0 41.0 34.5 41.0 18-19 38.52079663210735 40.0 38.0 41.0 35.0 41.0 20-21 38.47980065162999 40.0 38.0 41.0 34.0 41.0 22-23 38.348195415486316 40.0 38.0 41.0 34.0 41.0 24-25 38.475572407854216 40.0 38.0 41.0 34.0 41.0 26-27 38.44105334597037 40.0 38.0 41.0 34.5 41.0 28-29 38.346279513036535 40.0 38.0 41.0 34.0 41.0 30-31 38.12453037745706 40.0 37.5 41.0 34.0 41.0 32-33 37.860425948630976 40.0 37.0 41.0 34.0 41.0 34-35 37.61918961067602 40.0 37.0 41.0 33.0 41.0 36-37 37.424650359289146 40.0 36.0 41.0 33.0 41.0 38-39 37.20264918309962 39.0 36.0 41.0 33.0 41.0 40-41 37.0249106699817 39.0 36.0 41.0 33.0 41.0 42-43 36.75164548387859 39.0 35.5 41.0 31.5 41.0 44-45 36.36460430936935 39.0 35.0 40.5 30.5 41.0 46-47 35.87059355563665 39.0 35.0 40.5 29.5 41.0 48-49 35.59856756919959 39.0 35.0 41.0 27.5 41.0 50-51 34.69053745034668 38.0 34.0 40.0 25.5 40.5 52-53 34.83473158987742 38.5 35.0 39.5 26.5 40.5 54-55 35.264247514380756 39.0 35.0 40.5 27.0 41.0 56-57 35.1559763816945 39.0 35.0 41.0 26.5 41.0 58-59 34.931511245999516 39.0 35.0 40.5 26.0 41.0 60-61 34.61919617423358 38.0 35.0 40.0 24.5 41.0 62-63 34.32430866048292 38.0 34.5 40.0 23.0 41.0 64-65 34.017579176194765 37.5 34.0 40.0 22.5 41.0 66-67 33.57954375398736 37.0 34.0 39.5 21.5 41.0 68-69 33.17324378890549 36.5 33.5 39.0 20.0 41.0 70-71 32.70981986972651 36.0 33.0 39.0 18.0 40.5 72-73 32.293820935648256 35.5 33.0 38.5 15.5 40.0 74-75 31.86447500728555 35.0 32.0 37.0 11.5 39.5 76-77 31.423254946953328 35.0 32.0 37.0 7.5 39.0 78-79 30.95822361252957 35.0 31.5 36.5 4.5 39.0 80-81 30.422826346645103 35.0 31.0 36.0 2.0 37.5 82-83 29.91932993953651 34.0 30.5 35.5 2.0 37.0 84-85 29.570253694626548 34.0 30.0 35.0 2.0 37.0 86-87 29.320154584907492 34.0 30.0 35.0 2.0 36.0 88-89 28.90480544302701 34.0 29.0 35.0 2.0 36.0 90-91 28.47002685807751 34.0 29.0 35.0 2.0 36.0 92-93 28.13434420871063 34.0 29.0 35.0 2.0 35.0 94-95 27.754417930589064 34.0 28.5 35.0 2.0 35.0 96-97 27.23625787955084 34.0 27.0 35.0 2.0 35.0 98-99 26.623354844299286 34.0 25.5 35.0 2.0 35.0 100-101 24.221846276231258 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19925208980613007 1101 2 0.21345125384055308 1101 3 0.25944442513007004 1101 4 0.28420871360309974 1101 5 0.21477351256104527 1101 6 0.07242225557595816 1101 7 0.10006923239503607 1101 8 0.15369935074566854 1101 9 0.3094738720768646 1101 10-11 0.15049415368556396 1101 12-13 0.3320903118738059 1101 14-15 0.4359129290477384 1101 16-17 0.4586237677108542 1101 18-19 0.5247897752101309 1101 20-21 0.5395245497459555 1101 22-23 0.43295188385074823 1101 24-25 0.2127593332394042 1101 26-27 0.14624861442858617 1101 28-29 0.30627571576346924 1101 30-31 0.3844295981090582 1101 32-33 0.15990346842411896 1101 34-35 0.12708773925535866 1101 36-37 0.02723719388222179 1101 38-39 0.25516430106544163 1101 40-41 0.40299807801240917 1101 42-43 0.2092237054566226 1101 44-45 0.30290880615440585 1101 46-47 0.23544679760795617 1101 48-49 0.3175372620984902 1101 50-51 0.6164712024677925 1101 52-53 0.5670127567500955 1101 54-55 0.538968079778634 1101 56-57 0.342593177273514 1101 58-59 0.3619869425253839 1101 60-61 0.19078568581635835 1101 62-63 0.36490983830021406 1101 64-65 0.3014842981080932 1101 66-67 0.31802707003227937 1101 68-69 0.6340172617769326 1101 70-71 0.5982421528745334 1101 72-73 0.3856340820477655 1101 74-75 0.46058465119205394 1101 76-77 0.4449728572426892 1101 78-79 0.3600493640284199 1101 80-81 0.5138473557128656 1101 82-83 0.4480546866237347 1101 84-85 0.34267996263859857 1101 86-87 0.27706348144840476 1101 88-89 0.2456981133655809 1101 90-91 0.3053289029729207 1101 92-93 0.4058017251889119 1101 94-95 0.3795927201323508 1101 96-97 0.4080049071632672 1101 98-99 0.6041103432183199 1101 100-101 1.01098632667615 1102 1 0.13217192230150232 1102 2 0.11707676305294967 1102 3 0.05643465798237912 1102 4 0.1011260162043115 1102 5 0.16671501647158493 1102 6 0.0953443782663328 1102 7 0.1283347478728345 1102 8 0.1328941751422974 1102 9 0.355675292947069 1102 10-11 0.2627860930298098 1102 12-13 0.30123260418100983 1102 14-15 0.29817015947813985 1102 16-17 0.2765799644682687 1102 18-19 0.46951953813341873 1102 20-21 0.44330788068624827 1102 22-23 0.2822671392786873 1102 24-25 0.0782163102057325 1102 26-27 0.22034888225087457 1102 28-29 0.22460475152018233 1102 30-31 0.1546951539322663 1102 32-33 0.23336829613143806 1102 34-35 0.14106920172879 1102 36-37 0.20850731466924088 1102 38-39 0.1801794009262423 1102 40-41 0.37869590517507135 1102 42-43 0.38127430808643226 1102 44-45 0.15472725658236186 1102 46-47 0.34941247790860075 1102 48-49 0.387986270847513 1102 50-51 0.32249085542611766 1102 52-53 0.1772334342443571 1102 54-55 0.27762835130320695 1102 56-57 0.27472864764753524 1102 58-59 0.1213669523269445 1102 60-61 0.09142860169738043 1102 62-63 0.06241601767350602 1102 64-65 0.17267269571917154 1102 66-67 0.12504254249476077 1102 68-69 0.03951883228112507 1102 70-71 -0.11835096253823707 1102 72-73 -0.06619469205713102 1102 74-75 -0.1638091294452373 1102 76-77 -0.07843934344484182 1102 78-79 -0.1542014633490254 1102 80-81 0.1819909806050326 1102 82-83 0.2108937215142035 1102 84-85 0.07781535113517535 1102 86-87 0.2939011612079483 1102 88-89 0.4550658415514768 1102 90-91 0.41199918490423215 1102 92-93 0.06322177899069814 1102 94-95 0.02303783792313041 1102 96-97 0.1888332457751254 1102 98-99 0.4194440887076425 1102 100-101 0.7935596040163908 1103 1 0.051445685259032814 1103 2 0.19686634090397348 1103 3 0.04371160139635322 1103 4 0.05719657704285197 1103 5 0.04417636235606892 1103 6 0.012967602490974173 1103 7 0.04665092758339284 1103 8 0.04050216173120447 1103 9 0.2514045701317258 1103 10-11 0.2650914316179538 1103 12-13 0.1434418398129509 1103 14-15 0.17392988731771908 1103 16-17 0.20731026323916524 1103 18-19 0.20253659000784552 1103 20-21 0.26886279894704757 1103 22-23 0.2984309721717935 1103 24-25 0.3149566131368289 1103 26-27 0.2745489556081466 1103 28-29 0.009749164428107804 1103 30-31 0.06360904775129939 1103 32-33 0.2810203709361687 1103 34-35 0.12685807554688466 1103 36-37 0.07910803199509786 1103 38-39 0.08607961533627417 1103 40-41 -0.1801893103796246 1103 42-43 -0.05464577186808128 1103 44-45 -0.06397712589952675 1103 46-47 -0.15156479856710092 1103 48-49 0.05052679581975639 1103 50-51 -0.022433584116896554 1103 52-53 0.04437614368800524 1103 54-55 0.08445254738868613 1103 56-57 0.28297211011100387 1103 58-59 0.1862524485101531 1103 60-61 0.0964602216377628 1103 62-63 0.0933153089634331 1103 64-65 0.1567507202913916 1103 66-67 0.07287764124399132 1103 68-69 -0.028859134934570818 1103 70-71 0.10483116782601343 1103 72-73 0.1642511626412997 1103 74-75 -0.05622132841576999 1103 76-77 0.1924465564495499 1103 78-79 0.2219318204230163 1103 80-81 0.367512272677768 1103 82-83 0.34775673787681427 1103 84-85 0.46933458526551064 1103 86-87 0.1292220746783599 1103 88-89 0.2336647175604476 1103 90-91 0.4043409805820595 1103 92-93 0.44654001418200195 1103 94-95 0.019712759334840513 1103 96-97 0.07315522021823995 1103 98-99 0.12516817180322093 1103 100-101 0.6147955510514542 1104 1 0.4016270830288988 1104 2 0.36813094623631315 1104 3 0.28669960324825894 1104 4 0.34465907465603607 1104 5 0.4871374226502283 1104 6 0.25961625580544023 1104 7 0.36165135917145363 1104 8 0.3493034193526583 1104 9 0.5241525305511772 1104 10-11 0.4560652250677535 1104 12-13 0.3010329506683007 1104 14-15 0.4414942135564388 1104 16-17 0.27651867842164535 1104 18-19 0.4898898827631868 1104 20-21 0.6108348686602909 1104 22-23 0.4706326467334634 1104 24-25 0.37205636752398163 1104 26-27 0.2877300473428477 1104 28-29 0.5044406312782215 1104 30-31 0.4410210364829297 1104 32-33 0.1961525022749555 1104 34-35 0.2933542867268528 1104 36-37 0.30219851639383677 1104 38-39 0.42340034281064476 1104 40-41 0.41866135753559064 1104 42-43 0.4758184599444064 1104 44-45 0.6016232130932764 1104 46-47 0.4674083357300205 1104 48-49 0.6203747460380384 1104 50-51 0.672393113658984 1104 52-53 0.6377266795111183 1104 54-55 0.8539635973508908 1104 56-57 0.7707627454347943 1104 58-59 0.7013161796804894 1104 60-61 0.6414316975904555 1104 62-63 0.7816753789591715 1104 64-65 0.709518246036275 1104 66-67 0.8851525288349222 1104 68-69 0.696773141574738 1104 70-71 0.8146444340583017 1104 72-73 0.7753726638353626 1104 74-75 0.700521254154868 1104 76-77 0.9274390625113966 1104 78-79 1.0073213875009586 1104 80-81 0.9880718200595986 1104 82-83 1.0681648370845238 1104 84-85 0.9873337691717232 1104 86-87 1.1470800115828013 1104 88-89 1.1654267215938354 1104 90-91 1.360793745230552 1104 92-93 1.4178727581142674 1104 94-95 1.4312692489806516 1104 96-97 1.318837377844659 1104 98-99 1.3648394847987326 1104 100-101 1.9320193150747933 1105 1 0.2584674705582941 1105 2 0.1437588302517554 1105 3 0.2512569818710588 1105 4 0.28798050053384117 1105 5 0.35610284531918523 1105 6 0.2567118422761965 1105 7 0.27760849233154516 1105 8 0.35026881459562276 1105 9 0.4355898587101379 1105 10-11 0.3995512069528786 1105 12-13 0.2522427801471423 1105 14-15 0.10517396014018487 1105 16-17 0.12186140058940254 1105 18-19 0.11678519086002126 1105 20-21 0.20575488758422722 1105 22-23 0.08788360150113306 1105 24-25 0.2708537373704658 1105 26-27 0.1878438652479062 1105 28-29 0.018685010662089496 1105 30-31 0.057613081571268765 1105 32-33 0.1481734557398937 1105 34-35 0.05889488749340899 1105 36-37 0.006306931376663272 1105 38-39 0.08219071902681918 1105 40-41 -0.03197046896752198 1105 42-43 0.25419991448238477 1105 44-45 -0.07241876240158973 1105 46-47 0.0795196578689783 1105 48-49 0.05572000511090636 1105 50-51 0.2147443447436146 1105 52-53 0.24769603095474935 1105 54-55 0.22299689937286615 1105 56-57 0.3034504249893928 1105 58-59 0.34542404614328603 1105 60-61 0.12263597330404963 1105 62-63 0.0929419802605338 1105 64-65 0.004503401713115807 1105 66-67 0.27450986906132613 1105 68-69 0.4165767714059996 1105 70-71 0.09375953412892102 1105 72-73 -0.016257606242184863 1105 74-75 0.12442301421850033 1105 76-77 -0.09390590858845016 1105 78-79 0.007367326053000056 1105 80-81 -0.14006382894243785 1105 82-83 -0.009500798821598266 1105 84-85 0.06979072749105697 1105 86-87 -0.04034996640783106 1105 88-89 -0.22751176874193035 1105 90-91 -0.3139421080951941 1105 92-93 -0.39703258789902307 1105 94-95 -0.28614763309439795 1105 96-97 -0.11007237004182002 1105 98-99 0.13423892393700498 1105 100-101 0.5091268318560758 1106 1 0.14668536126487197 1106 2 0.2014003779951068 1106 3 0.28672658391305816 1106 4 0.32076272585000254 1106 5 0.2618488715403302 1106 6 0.12757998243166213 1106 7 0.3364088171952204 1106 8 0.3509606001855943 1106 9 0.5305341677947197 1106 10-11 0.40238506238853944 1106 12-13 0.4687071321601337 1106 14-15 0.5027374825905824 1106 16-17 0.39078108384732246 1106 18-19 0.23506602390330045 1106 20-21 0.36810260773737724 1106 22-23 0.12423570151273822 1106 24-25 0.16176480593991016 1106 26-27 0.09284444536161374 1106 28-29 0.26099125268552825 1106 30-31 0.27159382179635827 1106 32-33 0.10556918030189877 1106 34-35 0.076881312832775 1106 36-37 0.11219073460210183 1106 38-39 0.19885375998284616 1106 40-41 0.09578942197109797 1106 42-43 -0.09999720815365265 1106 44-45 0.033364971210197325 1106 46-47 0.03426571309580595 1106 48-49 0.360416750673032 1106 50-51 0.39788188970242544 1106 52-53 0.3929644785704838 1106 54-55 0.33933652693987426 1106 56-57 0.3843378539254587 1106 58-59 0.4436053896866241 1106 60-61 0.4175773818201165 1106 62-63 0.7537922819196581 1106 64-65 0.6109912483310538 1106 66-67 0.47729078912165335 1106 68-69 0.2918167934503231 1106 70-71 0.43682282453384147 1106 72-73 0.5380975973777211 1106 74-75 0.3155987596646064 1106 76-77 0.33755510777075415 1106 78-79 0.190009598938488 1106 80-81 0.576245342655195 1106 82-83 0.8180973854225151 1106 84-85 0.6005062977428253 1106 86-87 0.6850510371888703 1106 88-89 0.6574574852318484 1106 90-91 0.6169801276630125 1106 92-93 0.8322609786400186 1106 94-95 0.6847611150202297 1106 96-97 0.8400008693130907 1106 98-99 1.0971174481277473 1106 100-101 1.3843494504893314 1107 1 0.0856204148402071 1107 2 0.17624821675887148 1107 3 -0.03542244298394337 1107 4 -0.042510836362986026 1107 5 0.24215902534162126 1107 6 0.20884957205189636 1107 7 0.2233539298127596 1107 8 0.22013382709717888 1107 9 0.29650654187446435 1107 10-11 0.20677131503086343 1107 12-13 0.30903578220751626 1107 14-15 0.2309975598556946 1107 16-17 0.18747954947892964 1107 18-19 0.46377711800636945 1107 20-21 0.49315727644501095 1107 22-23 0.4391935154933506 1107 24-25 0.3929906605940374 1107 26-27 0.3032460781336752 1107 28-29 0.30321827360542386 1107 30-31 0.4478457352011205 1107 32-33 0.2801833103405329 1107 34-35 0.23114729431831194 1107 36-37 0.41395744500372444 1107 38-39 0.3498054510681996 1107 40-41 0.14343775639780887 1107 42-43 0.22365065247758764 1107 44-45 0.44613193673247054 1107 46-47 0.4639048085109252 1107 48-49 0.4193928968936049 1107 50-51 0.2930774214467249 1107 52-53 0.43944426937157033 1107 54-55 0.4282893478451868 1107 56-57 0.3271293292516475 1107 58-59 0.2891025104680196 1107 60-61 0.3206679860915713 1107 62-63 0.3801977232783571 1107 64-65 0.6252347245319427 1107 66-67 0.4330934168642031 1107 68-69 0.4491556118773232 1107 70-71 0.7500517406355911 1107 72-73 0.6795483226492465 1107 74-75 0.6280327016428835 1107 76-77 0.4527010732201333 1107 78-79 0.3466559470688182 1107 80-81 0.5801564331136824 1107 82-83 0.7346843268905516 1107 84-85 0.578333581072517 1107 86-87 0.5548187862959502 1107 88-89 0.7047459173333159 1107 90-91 0.5695573016552871 1107 92-93 0.6980379120899798 1107 94-95 0.7212205872849715 1107 96-97 0.8312971554855508 1107 98-99 1.1958309112081444 1107 100-101 1.4306925695213302 1108 1 -0.15918183097284455 1108 2 0.01971198098681981 1108 3 -0.12449486342876526 1108 4 -0.04428174554680453 1108 5 0.049851701538308646 1108 6 0.04587642471992126 1108 7 -0.20503043891959294 1108 8 -0.1640863979951206 1108 9 0.010143021645539818 1108 10-11 -0.19167446864036464 1108 12-13 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1307.0 12 2128.0 13 3179.0 14 3754.0 15 5303.0 16 7924.0 17 12151.0 18 9237.0 19 4288.0 20 3712.0 21 3690.0 22 3835.0 23 4230.0 24 4645.0 25 5314.0 26 6210.0 27 7291.0 28 8936.0 29 10945.0 30 13558.0 31 17829.0 32 23870.0 33 33559.0 34 49318.0 35 77225.0 36 113884.0 37 163083.0 38 136309.0 39 19799.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47696634967658 19.50290070882871 14.796372603862185 21.223760337632527 2 14.210610624279491 21.128813283369265 40.2798530489198 24.38072304343145 3 19.818813226634454 27.478418423281187 31.04005146754722 21.66271688253714 4 10.950957550477922 16.973373503786338 40.367627938252376 31.708041007483367 5 11.180093224357062 39.26429197164656 35.17965192562541 14.375962878370977 6 27.28873748105578 37.06763184782637 19.20700877015573 16.436621900962116 7 24.427958116145877 35.745870647550234 22.086601815772504 17.73956942053139 8 24.96359087834954 31.41020505985016 21.400815511796033 22.22538855000427 9 25.529858419886615 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20.635945825670188 34-35 20.15707665445762 26.542147480078242 32.68270909639899 20.618066769065155 36-37 19.527814171351153 25.914361181199734 34.34945794726243 20.20836670018668 38-39 19.71939801987477 26.88030173836957 33.330379490087886 20.069920751667773 40-41 18.98520817252406 27.799787863035135 33.7605609027373 19.454443061703504 42-43 18.635337020795635 27.722194545470057 32.00511177579074 21.637356657943567 44-45 18.773871167403875 27.39148156632271 31.73182509453294 22.102822171740478 46-47 19.96327018728529 27.747023743106595 31.695938060776157 20.593768008831958 48-49 19.84819704454191 27.45370364292962 31.197647080444803 21.500452232083667 50-51 18.933509587426276 28.17995770426856 32.1663500831606 20.720182625144563 52-53 19.35276159995379 29.096519308259367 30.260469347609053 21.29024974417779 54-55 18.755037794948944 27.88220946199632 31.48218163105093 21.880571112003803 56-57 20.136603681932012 27.736455328101563 29.992746416697024 22.134194573269397 58-59 20.63553119564147 27.715185926679574 30.405789490958025 21.243493386720928 60-61 20.392752516815417 27.734363974471155 31.69726929547548 20.175614213237953 62-63 19.735440393354047 28.43887777787259 31.31663464637322 20.50904718240015 64-65 19.077833290888996 29.389969833889484 30.883704261849193 20.64849261337233 66-67 19.358662739674415 28.8130475564713 29.4890030870255 22.339286616828787 68-69 18.905225733352765 28.59895256888455 30.62856370122087 21.867257996541824 70-71 20.27713049813912 28.274006051973455 29.60058287987292 21.848280570014506 72-73 19.65422407172816 27.769522096709615 31.33262882910084 21.24362500246139 74-75 19.13142905152168 29.43701206552746 30.443435373747796 20.988123509203067 76-77 18.917327602560206 28.636383626600047 30.365133304145598 22.081155466694145 78-79 19.209335481050598 28.248126983126763 30.967496741009715 21.57504079481292 80-81 20.141251597597712 28.58103127188799 29.61839487298319 21.65932225753111 82-83 19.529853631448464 28.08997540504226 30.983739234372816 21.39643172913646 84-85 19.66521593572146 28.548546971517098 30.037088822807327 21.749148269954116 86-87 19.163657176763717 29.295234569554335 30.540382300392384 21.000725953289567 88-89 19.33705900422978 28.827542658800485 29.27211575572832 22.563282581241417 90-91 18.765568397899862 28.555338003661262 29.69933423869345 22.97975935974543 92-93 19.759562741087162 29.07278107907265 30.1648123688709 21.002843810969285 94-95 19.341071074579798 28.2798426195418 30.137609439676734 22.24147686620167 96-97 18.364304703140895 28.407693065082544 31.30663953526535 21.921362696511206 98-99 18.851823526129387 29.022868340461567 29.49362725950823 22.631680873900812 100-101 18.69152803666831 27.94954102237668 30.625077557730858 22.733853383224158 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 917.0 1 689.0 2 774.5 3 966.5 4 703.5 5 656.0 6 1043.5 7 1887.0 8 2959.5 9 3322.5 10 2632.0 11 1996.5 12 1946.0 13 1989.5 14 2009.5 15 2035.5 16 2043.0 17 2124.5 18 2234.0 19 2282.0 20 2464.5 21 2730.5 22 3066.5 23 3557.0 24 4049.0 25 4793.0 26 5928.5 27 7172.0 28 8119.0 29 9409.0 30 11299.5 31 13234.0 32 15797.0 33 18573.0 34 21209.0 35 23063.0 36 25301.5 37 27122.5 38 26341.0 39 25023.0 40 24023.0 41 23582.0 42 23115.0 43 23450.0 44 24354.0 45 24626.5 46 25119.5 47 31942.5 48 50139.0 49 48310.0 50 28453.0 51 19909.0 52 16656.5 53 15165.5 54 13911.0 55 12271.0 56 10773.5 57 9406.0 58 8493.5 59 7587.0 60 6343.5 61 5272.0 62 4317.5 63 3073.0 64 2003.5 65 1529.0 66 1131.5 67 831.0 68 640.5 69 473.0 70 348.5 71 277.0 72 260.5 73 245.5 74 200.5 75 153.0 76 112.5 77 76.5 78 55.5 79 38.5 80 23.5 81 23.0 82 19.5 83 12.5 84 7.0 85 5.0 86 3.5 87 2.0 88 2.0 89 1.5 90 2.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03203016085966851 2 0.022053553378788157 3 0.017984147695797485 4 0.04725761438311748 5 0.05158956236823658 6 0.044107106757576314 7 0.04502600481502582 8 0.03898753186607192 9 0.04003770107458564 10-11 0.04391020003097999 12-13 0.03550884636287022 14-15 0.03025800032030161 16-17 0.037281006902237124 18-19 0.0414160481607599 20-21 0.008729531545770313 22-23 0.01562126697664161 24-25 0.004069405682990672 26-27 0.008073175790449236 28-29 0.004988303740440178 30-31 0.007351184459596052 32-33 0.002494151870220089 34-35 0.006826099855339191 36-37 0.00630101525108233 38-39 0.008204446941513451 40-41 0.0013127115106421523 42-43 0.001509618237238475 44-45 0.0013783470861742598 46-47 3.9381345319264565E-4 48-49 6.563557553210762E-4 50-51 3.9381345319264565E-4 52-53 0.0029536008989448424 54-55 0.005250846042568609 56-57 0.011748768020247264 58-59 0.007482455610660268 60-61 0.007285548884063945 62-63 0.0033474143521374884 64-65 0.0 66-67 0.0059728373734217926 68-69 0.01430855546599946 70-71 0.006169744100018116 72-73 0.0022316095680916586 74-75 0.0032817787766053807 76-77 0.006104108524486008 78-79 0.005644659495761254 80-81 0.012011310322375693 82-83 0.005316481618100717 84-85 0.012733301653228876 86-87 0.0032817787766053807 88-89 0.005250846042568609 90-91 0.0029536008989448424 92-93 0.01680270733621955 94-95 0.008007540214917128 96-97 0.007482455610660268 98-99 0.01831232557345802 100-101 0.033145965643714345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 761782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.88987502222038 #Duplication Level Percentage of deduplicated Percentage of total 1 79.94014417826047 42.28024234846474 2 11.745523103728734 12.424384980536296 3 3.633913664065896 5.765917186019527 4 1.537931985896202 3.2536412210690124 5 0.8079942499365553 2.136735744890856 6 0.4591083087740072 1.4569308643632124 7 0.31696446852545795 1.1734947788757184 8 0.20720312262483606 0.8767157807873106 9 0.16149058133779115 0.7687094997799341 >10 1.0326820954498237 10.500073964638402 >50 0.09586151938744346 3.481599742829908 >100 0.05077918223759663 4.513686861524987 >500 0.0042109565756802275 1.586792884968238 >1k 0.004954066559623797 4.952177266495988 >5k 9.908133119247594E-4 3.498621531792999 >10k+ 2.4770332798118984E-4 1.33027534296297 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10154 1.3329272679060413 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8052 1.056995308369061 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6523 0.856281718391876 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6317 0.8292398612726475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5813 0.763079201136283 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4208 0.5523890036782176 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3874 0.5085444392227698 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3704 0.48622834354185324 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3637 0.4774331764205508 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2002 0.2628048444305589 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1969 0.25847289644543975 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1735 0.22775544709641343 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1680 0.22053553378788157 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1443 0.18942427098566256 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1431 0.18784901717289199 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 0.18758647487076355 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1423 0.18679884796437826 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1391 0.18259817113032337 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1262 0.1656641926430396 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1216 0.1596257196940857 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1132 0.14859894300469162 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1119 0.14689241804085684 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1102 0.1446608084727652 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1025 0.1345529298408206 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1018 0.1336340317833711 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 920 0.12076945897907801 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 919 0.12063818782801379 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 910 0.11945674746843586 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 801 0.10514819200243639 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 780 0.10239149783008789 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 776 0.10186641322583101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.563557553210761E-5 0.0 12-13 0.0 0.0 0.0 1.3127115106421522E-4 0.0 14-15 0.0 0.0 0.0 2.6254230212843045E-4 0.0 16-17 0.0 0.0 0.0 5.250846042568609E-4 0.0 18-19 0.0 0.0 0.0 5.250846042568609E-4 0.0 20-21 0.0 0.0 0.0 5.250846042568609E-4 0.0 22-23 0.0 0.0 0.0 7.876269063852913E-4 0.0 24-25 0.0 0.0 0.0 0.0016408893883026903 0.0 26-27 0.0 0.0 0.0 0.002428516294687982 0.0 28-29 0.0 0.0 0.0 0.0043975835606512095 0.0 30-31 0.0 0.0 0.0 0.008007540214917128 0.0 32-33 0.0 0.0 0.0 0.012667666077696768 0.0 34-35 0.0 0.0 0.0 0.018903045753246993 0.0 36-37 0.0 0.0 0.0 0.026713679241567798 0.0 38-39 0.0 0.0 0.0 0.040300243376714076 0.0 40-41 0.0 0.0 0.0 0.06268197463316277 0.0 42-43 0.0 0.0 0.0 0.08237264729279506 0.0 44-45 0.0 0.0 0.0 0.10974268228968392 0.0 46-47 0.0 0.0 0.0 0.14971474778873745 0.0 48-49 0.0 0.0 0.0 0.18857100850374517 0.0 50-51 0.0 0.0 0.0 0.22289841450703746 0.0 52-53 0.0 0.0 0.0 0.25505984651777014 0.0 54-55 0.0 0.0 0.0 0.2960820812253374 0.0 56-57 0.0 0.0 0.0 0.3615207500308487 0.0 58-59 0.0 0.0 0.0 0.4282721303470022 0.0 60-61 0.0 0.0 0.0 0.502571601849348 0.0 62-63 0.0 0.0 0.0 0.5785119627399964 0.0 64-65 0.0 0.0 0.0 0.6549117726593697 0.0 66-67 0.0 0.0 0.0 0.7451606890160176 0.0 68-69 0.0 0.0 0.0 0.8568068029961327 0.0 70-71 0.0 0.0 0.0 0.9840741839528895 0.0 72-73 0.0 0.0 0.0 1.1062219900181418 0.0 74-75 0.0 0.0 0.0 1.2397247506504485 0.0 76-77 0.0 0.0 0.0 1.3829415764615074 0.0 78-79 0.0 0.0 0.0 1.5633737735992712 0.0 80-81 0.0 0.0 0.0 1.7595585088647407 0.0 82-83 0.0 0.0 0.0 1.9533147278355225 0.0 84-85 0.0 0.0 0.0 2.1762131423425597 0.0 86-87 0.0 0.0 0.0 2.4010149885400285 0.0 88-89 0.0 0.0 0.0 2.6471483967854317 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 85 6.3875417E-4 27.954353 9 ATACGCT 85 6.3875417E-4 27.954353 8 CCGATGT 70 0.008370546 27.155659 9 ATAACGC 90 8.916747E-4 26.40307 3 GTACATG 6515 0.0 25.898188 1 TACATGG 6515 0.0 25.4588 2 ACATGGG 6555 0.0 24.578419 3 GAGTACT 5730 0.0 23.513165 12-13 ATGGGCG 125 2.246744E-4 22.81225 5 GTGTAGC 335 0.0 22.700249 1 CATGGGT 610 0.0 22.595585 4 CATGGGA 4415 0.0 21.961143 4 GTACTTT 6165 0.0 21.854803 14-15 AGTACTT 5875 0.0 21.800354 12-13 GTATCAA 12560 0.0 21.683052 1 CATGGGG 1660 0.0 21.473785 4 ATGGGAT 1650 0.0 20.450377 5 CCATCCG 140 4.8342702E-4 20.366745 9 GGGCGTA 70 4.8416143E-4 20.364069 18-19 TACCTGG 565 0.0 20.187834 2 >>END_MODULE