FastQCFastQC Report
Thu 26 May 2016
SRR936677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761782
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT163702.1489087429212033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74890.9830896503199078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67490.8859489985323885No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC50610.6643632955359933No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC47550.6241943233103434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47190.6194685618720317No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40120.5266598580696314No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33420.43870818685660723No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30070.39473235125009515No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25570.3356603332711983No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25160.3302782160775655No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23600.3097999165115479No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC22390.2939161072327779No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.2364193430666516No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14580.1913933382516258No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.18811155947502042No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT13960.18325452688564445No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG13890.18233562882819496No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13490.17708478278562634No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12050.15818173703237934No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12030.15791919473025093No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8900.11683132444715155No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.1138120879726746No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA8450.11092412264926187No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG8440.11079285149819765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG250.00502046357.0004735
TATCACG600.00394996231.666932
GCAACGC350.008386699527.14664684-85
GTAAGCG1354.0017767E-1122.8705694-95
CATGGGT5250.022.6192364
GGGATCG1050.00219544122.6192369
AGTACGC2154.4161425E-822.0932062
AGTACTT77850.021.5994112-13
CGGTATA1100.002876346721.5910872
CGTATAG1553.8940758E-521.4588341
GAGTACT77550.020.886712-13
GTGTAGC3001.6552804E-1020.5902611
GTACTTT80650.020.05441314-15
GACATAT3353.45608E-1119.8574281
TATCGTG1200.00476182219.791836
ATAGCCC1707.948856E-519.5589878
GAGTACG2451.7478669E-719.3942831
GTACGCA2502.1679443E-719.0014043
TACGCAG2502.1694541E-719.0001564
TACTTTT88900.018.8879514-15
Produced by FastQC (version 0.11.5)