##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936677_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 761782 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.850499224187498 31.0 31.0 31.0 28.0 34.0 2 30.933973236437723 31.0 31.0 33.0 28.0 34.0 3 30.954056409839037 31.0 31.0 34.0 27.0 34.0 4 34.807607688288776 35.0 35.0 37.0 32.0 37.0 5 34.523823088495135 35.0 35.0 37.0 30.0 37.0 6 34.79048074120943 35.0 35.0 37.0 32.0 37.0 7 34.71978072466926 35.0 35.0 37.0 32.0 37.0 8 34.72452486406873 35.0 35.0 37.0 31.0 37.0 9 36.073794077570746 37.0 35.0 39.0 31.0 39.0 10-11 35.87589100293785 37.0 35.0 39.0 30.0 39.0 12-13 35.741717446723605 37.0 35.0 39.0 30.0 39.0 14-15 36.3055447621498 38.0 34.5 40.0 30.0 41.0 16-17 36.25228805616305 38.0 34.5 40.0 30.0 41.0 18-19 36.5894534131812 38.0 35.0 40.0 30.5 41.0 20-21 36.69175236484978 38.0 35.0 40.0 30.5 41.0 22-23 36.54499318702726 38.0 35.0 40.0 30.0 41.0 24-25 36.29271497620054 38.0 34.5 40.0 30.0 41.0 26-27 35.84239979416684 38.0 34.0 40.0 28.0 41.0 28-29 35.6440976552347 38.0 34.0 40.0 27.5 41.0 30-31 35.46138002735691 38.0 34.0 40.0 27.0 41.0 32-33 35.1140437815543 37.0 33.5 40.0 27.0 41.0 34-35 34.74611765570728 37.0 33.0 40.0 26.5 41.0 36-37 34.28200062485068 37.0 33.0 40.0 24.5 41.0 38-39 33.9604473458286 37.0 33.0 39.0 24.0 40.5 40-41 33.6432719596945 37.0 32.5 39.0 23.5 40.0 42-43 33.13057738303084 36.0 31.5 39.0 21.5 40.0 44-45 32.58721655276706 36.0 31.0 39.0 18.0 40.0 46-47 31.849918349344037 36.0 30.0 39.0 11.5 40.0 48-49 31.141153899672084 35.0 29.5 39.0 7.5 40.0 50-51 30.591510432118376 35.0 28.0 39.0 2.0 40.0 52-53 30.245408135135776 35.0 28.0 38.0 2.0 40.0 54-55 29.868532598564943 35.0 27.0 38.0 2.0 40.0 56-57 29.396414591050984 34.0 26.5 38.0 2.0 40.0 58-59 28.92931311057494 34.0 26.0 38.0 2.0 40.0 60-61 28.309294522579954 33.5 25.0 38.0 2.0 39.5 62-63 27.832319482476613 33.0 23.5 37.0 2.0 39.0 64-65 27.277511151484283 32.5 22.5 36.5 2.0 39.0 66-67 26.617639954737708 32.0 22.0 36.0 2.0 39.0 68-69 26.137348349002735 31.0 20.5 36.0 2.0 38.5 70-71 25.50751199161965 31.0 18.5 35.0 2.0 38.0 72-73 24.871624690528265 30.5 16.5 35.0 2.0 37.5 74-75 24.186061104095398 30.0 12.5 34.0 2.0 37.0 76-77 23.382166420314473 28.5 13.0 33.5 2.0 35.5 78-79 23.992270754625338 30.0 10.0 34.0 2.0 36.0 80-81 23.590741839528896 30.0 7.0 34.0 2.0 36.0 82-83 23.031611405887773 29.5 4.5 34.0 2.0 35.0 84-85 22.335005290227386 29.0 2.0 34.0 2.0 35.0 86-87 21.755113667689706 29.0 2.0 34.0 2.0 35.0 88-89 20.999260287063752 28.5 2.0 33.0 2.0 35.0 90-91 20.107597711681294 26.5 2.0 33.0 2.0 34.5 92-93 19.215662748660378 25.5 2.0 32.5 2.0 34.0 94-95 18.170664967142834 24.5 2.0 32.0 2.0 34.0 96-97 16.885051760214864 19.0 2.0 32.0 2.0 34.0 98-99 15.520536715228241 2.0 2.0 31.0 2.0 34.0 100-101 12.774789900522723 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22937726313347184 1101 2 0.25332393970535705 1101 3 0.2831859798169596 1101 4 0.2089872471358376 1101 5 0.09617868859510281 1101 6 -0.15203970133401867 1101 7 -0.12767844425045638 1101 8 -0.16416010527365188 1101 9 0.22904729859141781 1101 10-11 0.14343480407859488 1101 12-13 0.19463463046070473 1101 14-15 0.2485746695806128 1101 16-17 0.42070690249828857 1101 18-19 0.3247246054549322 1101 20-21 0.27666674966982896 1101 22-23 0.40762896949729566 1101 24-25 0.1243696696221619 1101 26-27 0.23399668990807498 1101 28-29 0.21341223588218128 1101 30-31 0.24036421353995507 1101 32-33 0.3430297599798706 1101 34-35 0.5811165797597297 1101 36-37 0.6522743395958486 1101 38-39 0.7948187499083446 1101 40-41 0.4966021331319439 1101 42-43 0.7144370028988973 1101 44-45 0.5805776308171886 1101 46-47 0.4751357453257192 1101 48-49 0.8109792846082478 1101 50-51 1.0855378257111532 1101 52-53 0.7741456585768915 1101 54-55 0.8139965412999182 1101 56-57 0.8780938447011337 1101 58-59 0.735256466818246 1101 60-61 0.6254948384251371 1101 62-63 0.6638603904468212 1101 64-65 0.3436267693581705 1101 66-67 0.12002520094036484 1101 68-69 0.26525165138368934 1101 70-71 0.7411302497410475 1101 72-73 0.7010854610352446 1101 74-75 0.5605724743431999 1101 76-77 0.46242700192719965 1101 78-79 0.37041309298561487 1101 80-81 0.7047796697679871 1101 82-83 0.9813263661105047 1101 84-85 0.851719654394568 1101 86-87 0.8150211716117113 1101 88-89 0.39660631877499597 1101 90-91 0.6335900420172322 1101 92-93 1.0174641027254516 1101 94-95 0.844971493697745 1101 96-97 0.7627697767747392 1101 98-99 0.9821295861619301 1101 100-101 1.1991509748693954 1102 1 0.2425512242096488 1102 2 0.3899726368454637 1102 3 0.49953464954592164 1102 4 0.3997913429063331 1102 5 0.2500679262212273 1102 6 0.30833102546470315 1102 7 0.40676407126176173 1102 8 0.45199965303364564 1102 9 0.46181298791044867 1102 10-11 0.4791963769797718 1102 12-13 0.4141367681738046 1102 14-15 0.5571695459516093 1102 16-17 0.645554212943118 1102 18-19 0.41105131067124034 1102 20-21 0.44546717400854874 1102 22-23 0.4820431008310848 1102 24-25 0.5563568113045463 1102 26-27 0.3702917714473202 1102 28-29 0.3127135484131074 1102 30-31 0.3746883034247688 1102 32-33 0.46865170218389807 1102 34-35 0.4485771806926593 1102 36-37 0.6513434287271593 1102 38-39 0.5350578320266663 1102 40-41 0.5380463562040632 1102 42-43 0.5702977068312762 1102 44-45 0.9387528556538172 1102 46-47 0.9088624859035797 1102 48-49 0.48326899308897353 1102 50-51 0.533706441021284 1102 52-53 0.6369517051663394 1102 54-55 0.8472286946884005 1102 56-57 0.7205990332387664 1102 58-59 0.12173043275960538 1102 60-61 0.48750524991266175 1102 62-63 0.38317622732323287 1102 64-65 0.688089896625371 1102 66-67 0.5508120668500425 1102 68-69 0.521999123566129 1102 70-71 0.49806817596354236 1102 72-73 0.6495679398568726 1102 74-75 0.8268390886399466 1102 76-77 0.47038691283505685 1102 78-79 0.5850844618695774 1102 80-81 0.8625450327638937 1102 82-83 0.5354378594481766 1102 84-85 0.4340486047139507 1102 86-87 0.759138040381167 1102 88-89 0.932522628167284 1102 90-91 0.7209615745374052 1102 92-93 0.9233008960727282 1102 94-95 1.1924806989763432 1102 96-97 1.7838994955554064 1102 98-99 1.6437384422154597 1102 100-101 1.5074017286758075 1103 1 0.04238924740334937 1103 2 0.10666830999694454 1103 3 0.2521064621353126 1103 4 0.09099782394790878 1103 5 0.06617994130623828 1103 6 0.2657247251085053 1103 7 0.16902864486643665 1103 8 0.21710989818972593 1103 9 0.4179426284351706 1103 10-11 0.11548952053602335 1103 12-13 0.08168552737731005 1103 14-15 0.04281256679443146 1103 16-17 0.2638809777641242 1103 18-19 -0.1375595782072594 1103 20-21 0.2293019872059645 1103 22-23 0.1403298883977584 1103 24-25 0.39630132563259934 1103 26-27 0.3550042872326813 1103 28-29 0.23455050817245393 1103 30-31 0.259428143564989 1103 32-33 0.5935633903613109 1103 34-35 0.6309050926442339 1103 36-37 0.5153272211999109 1103 38-39 0.5898948582506662 1103 40-41 0.9346064414757365 1103 42-43 0.7210401415543757 1103 44-45 0.42269979717231365 1103 46-47 0.4663913303295395 1103 48-49 0.5740486233978324 1103 50-51 0.8784444103047697 1103 52-53 0.8130134779015563 1103 54-55 0.7316817521852421 1103 56-57 0.6058305886838191 1103 58-59 0.7259950014409711 1103 60-61 1.0065879885515123 1103 62-63 0.5403934264045418 1103 64-65 0.6888856261487213 1103 66-67 0.8249018088933617 1103 68-69 0.882314436774621 1103 70-71 0.5366712121670822 1103 72-73 0.43247840213080124 1103 74-75 0.6127699998702596 1103 76-77 0.719692726522343 1103 78-79 0.7494595341806516 1103 80-81 0.693852835467279 1103 82-83 0.653147980044583 1103 84-85 0.5011871293464836 1103 86-87 0.5576400010882523 1103 88-89 0.46735318024233763 1103 90-91 0.5607626975437761 1103 92-93 0.4446803575121585 1103 94-95 0.6604207380181712 1103 96-97 0.924680849021783 1103 98-99 1.2518505754598461 1103 100-101 1.6077153433433242 1104 1 0.13117868212761508 1104 2 0.22388239598498316 1104 3 0.15221082797550878 1104 4 0.140720692099066 1104 5 -0.0298235532492086 1104 6 0.16253594325926457 1104 7 0.05232854102373352 1104 8 0.1898583817462196 1104 9 0.06855116760113589 1104 10-11 0.3583990518313911 1104 12-13 0.16443403709065763 1104 14-15 0.3667462173231897 1104 16-17 0.27896825010142123 1104 18-19 0.07214579100978824 1104 20-21 0.11284252640331971 1104 22-23 0.023686021787241884 1104 24-25 0.1477785338594515 1104 26-27 0.1859231251360498 1104 28-29 0.3271555737084526 1104 30-31 0.18115834086611926 1104 32-33 0.49536925943580457 1104 34-35 0.3678122802856407 1104 36-37 0.39282814322894666 1104 38-39 0.3404487295955718 1104 40-41 0.11609445248254247 1104 42-43 0.6826634619575103 1104 44-45 0.989044690962416 1104 46-47 0.951228203120916 1104 48-49 0.8500413413301864 1104 50-51 0.990725967893006 1104 52-53 0.8290796704316854 1104 54-55 0.9023728685335364 1104 56-57 1.1980975769053934 1104 58-59 0.7817287142230427 1104 60-61 0.864173083330865 1104 62-63 1.228928093388582 1104 64-65 1.5486502406359541 1104 66-67 1.1702958487930246 1104 68-69 1.507138204983061 1104 70-71 1.1422173803190212 1104 72-73 1.3502582608314846 1104 74-75 1.5879903059577423 1104 76-77 1.3284509561219586 1104 78-79 1.2723853611949245 1104 80-81 1.4525009447192225 1104 82-83 1.8693526731070023 1104 84-85 1.4871520163881193 1104 86-87 1.3557450679320837 1104 88-89 1.7786308378439095 1104 90-91 2.012908082559562 1104 92-93 2.077447307806292 1104 94-95 2.116867617374922 1104 96-97 1.856079926731173 1104 98-99 1.8934493868924829 1104 100-101 2.0486832515013855 1105 1 -0.02508507372612101 1105 2 0.07149393410369598 1105 3 -0.2783595642848731 1105 4 0.05726199908515639 1105 5 -0.2040485973022257 1105 6 0.03484596677706264 1105 7 -0.17371381843515366 1105 8 0.05913508952902902 1105 9 -0.17271947903540763 1105 10-11 -0.11803927052011431 1105 12-13 -0.41188841040568747 1105 14-15 0.006344563535314762 1105 16-17 0.1607594422411296 1105 18-19 -0.24808157054032876 1105 20-21 0.19942366965617708 1105 22-23 -0.028705868344196972 1105 24-25 0.02601668754105191 1105 26-27 -0.05670647986097066 1105 28-29 -0.39286677848254925 1105 30-31 -0.06439076196081572 1105 32-33 -0.06794435003165233 1105 34-35 -0.11702456928959748 1105 36-37 -0.6306663407165587 1105 38-39 -0.39326193980454605 1105 40-41 -0.3895978140707612 1105 42-43 -0.15834663766744228 1105 44-45 -0.1182067437602825 1105 46-47 0.12118850398415404 1105 48-49 -0.09716566327052334 1105 50-51 -0.024624364990085468 1105 52-53 -0.13692374410546648 1105 54-55 -0.039809692643590466 1105 56-57 -0.5593516678511001 1105 58-59 -0.08110425457302739 1105 60-61 -0.28083504162199446 1105 62-63 -0.5282496339558307 1105 64-65 -0.4992259583945362 1105 66-67 -0.1223800375810491 1105 68-69 -0.5167779537312107 1105 70-71 -0.4882817043381884 1105 72-73 -0.22191806093035993 1105 74-75 -0.42799562450363027 1105 76-77 -0.48778386530048934 1105 78-79 -0.3928000728558274 1105 80-81 -0.07294695616321079 1105 82-83 -0.11222528187321501 1105 84-85 -0.5378200433731379 1105 86-87 -0.7956992538571868 1105 88-89 -0.302086626348018 1105 90-91 0.2802512572563316 1105 92-93 0.014667134062573695 1105 94-95 0.44809554801456386 1105 96-97 0.6366280353655682 1105 98-99 0.9072501507066182 1105 100-101 0.8271271001921345 1106 1 0.13764552147053877 1106 2 0.057004144104855214 1106 3 0.08977188948006543 1106 4 0.21305443440247274 1106 5 0.13967892329181097 1106 6 0.1319526496869159 1106 7 0.004447801242299931 1106 8 0.05575737749747134 1106 9 0.0709297262088171 1106 10-11 0.16121441320188978 1106 12-13 0.16871331253200594 1106 14-15 0.3246130691624174 1106 16-17 0.38274937726839653 1106 18-19 0.28069496966154617 1106 20-21 0.069784935464277 1106 22-23 0.22379877256542358 1106 24-25 -0.11330341919987319 1106 26-27 0.036206706123486754 1106 28-29 0.29895651888373465 1106 30-31 0.4395337072351495 1106 32-33 0.2374035731612949 1106 34-35 0.253617326521983 1106 36-37 0.008631286618403067 1106 38-39 0.12926822610796762 1106 40-41 0.16170526633300142 1106 42-43 0.4260787597104425 1106 44-45 0.23958998439844237 1106 46-47 0.060567347237430624 1106 48-49 0.18440775011849908 1106 50-51 0.17498260797661658 1106 52-53 -0.20600513405789656 1106 54-55 -0.06571539516128055 1106 56-57 0.16115607666682408 1106 58-59 -0.2222480937655078 1106 60-61 -0.06890137762384896 1106 62-63 -0.28215669974072455 1106 64-65 0.1915966428353073 1106 66-67 0.26907561272640734 1106 68-69 0.22423173416762054 1106 70-71 -0.06674715856721392 1106 72-73 -0.017591862995566032 1106 74-75 -0.02389567091272582 1106 76-77 -0.30790293363788024 1106 78-79 0.07287967537482487 1106 80-81 0.37916465771310826 1106 82-83 0.7263537296130345 1106 84-85 0.3508322074680308 1106 86-87 0.2266525270918791 1106 88-89 0.35238532515447574 1106 90-91 0.6969041462225718 1106 92-93 0.9253629804484653 1106 94-95 1.0830793048308998 1106 96-97 0.7322692060350171 1106 98-99 1.2992975169904089 1106 100-101 0.8006166291398085 1107 1 0.05776542816927588 1107 2 0.2721391758127041 1107 3 0.23973726596523193 1107 4 0.1274159038973366 1107 5 0.1984341168190369 1107 6 0.23452481238098954 1107 7 -0.12186424774085935 1107 8 -0.021612510978364696 1107 9 0.08776313243376421 1107 10-11 0.03876106383240341 1107 12-13 0.02471365876715481 1107 14-15 0.22746449783797829 1107 16-17 0.3455632667647137 1107 18-19 0.20830448930583856 1107 20-21 0.23297330560787088 1107 22-23 0.35659361912858145 1107 24-25 0.3510161376191405 1107 26-27 0.34431270874758724 1107 28-29 0.29683355073737516 1107 30-31 0.18560667173693446 1107 32-33 0.29710407975798603 1107 34-35 0.4731076319378289 1107 36-37 0.7225294388160819 1107 38-39 0.508892287628484 1107 40-41 0.45601197600571197 1107 42-43 0.35279991285571555 1107 44-45 0.27328413120214634 1107 46-47 0.44135084093230503 1107 48-49 0.2557390242638462 1107 50-51 0.3629290690889455 1107 52-53 0.3017988239565348 1107 54-55 0.3623701978119662 1107 56-57 0.11765573043167521 1107 58-59 0.42820702322552506 1107 60-61 0.48356008093727 1107 62-63 0.3086024663792557 1107 64-65 0.2753126571220612 1107 66-67 0.9976796472770317 1107 68-69 0.8848256185615249 1107 70-71 0.44903159717300056 1107 72-73 0.7097251790628931 1107 74-75 0.5925714549710825 1107 76-77 0.4813332534019885 1107 78-79 0.6149039665039169 1107 80-81 0.9310088154505536 1107 82-83 0.7170554935900029 1107 84-85 0.8780595017314567 1107 86-87 1.0409064814617324 1107 88-89 1.2477516966101199 1107 90-91 0.9566420097694746 1107 92-93 1.0968086033072737 1107 94-95 1.4339339589602105 1107 96-97 1.3985235795316306 1107 98-99 1.4219535961506988 1107 100-101 1.4615954723499556 1108 1 -0.2116570861704581 1108 2 -0.09984596707363735 1108 3 -0.19360692596363904 1108 4 0.01912376935295157 1108 5 -0.19528418566311245 1108 6 0.04246659753409432 1108 7 0.03403644082184343 1108 8 0.06757009227410293 1108 9 -0.004330532533856513 1108 10-11 -0.27876183502935703 1108 12-13 -0.019904789347926055 1108 14-15 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44.0 5 90.0 6 247.0 7 593.0 8 1189.0 9 1940.0 10 3055.0 11 4675.0 12 6384.0 13 8571.0 14 10994.0 15 14589.0 16 17654.0 17 20639.0 18 16727.0 19 12722.0 20 12472.0 21 13074.0 22 13787.0 23 15353.0 24 17343.0 25 19786.0 26 22994.0 27 26769.0 28 30867.0 29 34850.0 30 40040.0 31 45218.0 32 50539.0 33 56428.0 34 63589.0 35 67102.0 36 61235.0 37 40284.0 38 9754.0 39 164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14209289896617 23.963641950423945 16.44424368087171 16.450021469738175 2 17.42670282844248 27.99653960160785 35.596157329979704 18.980600239969963 3 24.787663338418035 28.03145864456352 30.41829337772165 16.762584639296787 4 14.710303119408646 20.70915580503007 37.40139856835119 27.179142507210095 5 11.350610548535393 42.79641993557144 32.65233520702991 13.20063430886326 6 26.95443053261957 42.0301346054383 15.801764809354907 15.213670052587222 7 25.47881152350672 37.40715847840983 20.45138898004941 16.66264101803403 8 26.632291127907987 33.79129462234602 18.500699675235172 21.07571457451082 9 26.131452785902194 19.222095032057172 21.024490524624426 33.62196165741621 10-11 24.8124944619656 29.373012460987624 25.91662263587465 19.897870441172127 12-13 28.73713124895393 26.89413903666097 26.388479575729136 17.980250138655972 14-15 23.23497655124431 28.34414700023301 28.977936181315165 19.442940267207515 16-17 19.172842018207714 31.080648231409953 29.339035004233587 20.407474746148747 18-19 18.997560982013226 29.716966271190447 33.06011431091835 18.22535843587798 20-21 21.472692629054393 26.506616398534227 33.31624619395457 18.704444778456814 22-23 20.45037452446027 27.15820868875744 33.17068496099852 19.220731825783773 24-25 21.113465992888973 27.81183933040552 33.64683380711746 17.427860869588045 26-27 20.978134156715715 26.252271128054865 35.15364903326087 17.615945681968554 28-29 18.984933040842396 29.45855043696413 33.99780500516585 17.55871151702762 30-31 20.180460938746066 27.35439695499241 33.45005687401012 19.0150852322514 32-33 20.075126824923515 28.195334995671313 33.598939849313155 18.130598330092017 34-35 21.24886520209085 27.036121200027047 34.06034794561372 17.65466565226838 36-37 21.06153840094422 26.705129534835176 34.20998389102085 18.023348173199754 38-39 19.52483041379428 28.088884571101485 34.86813170943683 17.518153305667404 40-41 20.18266728365282 28.150058909050337 32.846964460941166 18.82030934635568 42-43 19.409881730868317 28.990738133541267 32.247389172326116 19.351990963264303 44-45 21.219637544132098 26.899001083652596 31.990330484506263 19.891030887709043 46-47 21.847260764890745 29.135828885429166 30.701368633020998 18.315541716659098 48-49 21.744698084018083 29.005423506763467 30.544720137730685 18.705158271487765 50-51 19.99397461461819 29.654282027417473 31.036051232841107 19.31569212512323 52-53 21.37143937335334 30.889350971226186 28.80715807547957 18.9320515799409 54-55 20.50939727973136 29.346660312984486 29.266189090307616 20.87775331697654 56-57 21.589942450274098 29.41940938018525 29.39669929218038 19.593948877360273 58-59 23.488597698010963 28.412682993425886 28.711458486484215 19.38726082207893 60-61 20.882816074186533 30.098568372660257 30.39748318193961 18.621132371213598 62-63 20.847245915821517 29.139987436831348 30.48563801402165 19.52712863332548 64-65 20.698799625321886 30.90273925934775 29.140413051768988 19.258048063561372 66-67 21.56300581676978 29.18663678860748 28.88418051283667 20.366176881786068 68-69 21.01070672806801 29.65373790307759 28.75155559989708 20.583999768957316 70-71 21.980843588351778 30.135150302284842 28.831036196074596 19.052969913288784 72-73 22.05474057300384 28.394604706114013 29.791605132749172 19.759049588132978 74-75 20.43420184642448 31.90507290849598 28.24609366025921 19.41463158482033 76-77 21.49362238116586 29.422309096434535 28.400012864851455 20.684055657548157 78-79 21.182628147148517 29.908265190788185 28.91976617337984 19.989340488683457 80-81 21.685620854113363 29.78074142532514 28.912476419397994 19.621161301163507 82-83 21.503947132642953 29.522005420374388 29.121745757004707 19.852301689977953 84-85 20.030627107418706 30.49661729965533 29.34675517414983 20.126000418776137 86-87 20.907810374975714 30.25312567278761 27.739722430804616 21.09934152143206 88-89 20.415571287443917 30.361187965433604 27.898480089533916 21.324760657588556 90-91 21.46766190382102 28.62709393332891 28.448741588655295 21.456502574194772 92-93 21.7513045190509 30.51281546388369 28.072987430671787 19.66289258639362 94-95 21.155363371310727 29.986912042889642 28.334639503576543 20.523085082223087 96-97 19.765324714876815 30.074523219424083 29.056573060847285 21.10357900485182 98-99 20.930594327219644 30.532037300437604 27.368838368489833 21.16853000385292 100-101 20.677505298650935 29.89285861971866 27.27841990257965 22.151216179050753 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1443.0 1 1014.5 2 742.0 3 840.5 4 683.0 5 631.0 6 993.0 7 1805.0 8 2536.0 9 2829.0 10 2493.0 11 1995.0 12 1892.5 13 1950.5 14 2071.5 15 2164.0 16 2311.0 17 2501.0 18 2634.0 19 2799.0 20 3081.5 21 3422.0 22 3782.5 23 4236.5 24 4781.5 25 5507.5 26 6649.0 27 7927.0 28 8932.5 29 10228.0 30 12079.0 31 14000.5 32 16536.5 33 19324.5 34 21576.0 35 23183.5 36 25211.0 37 27015.0 38 26688.0 39 25612.0 40 24672.0 41 23840.0 42 23378.0 43 23199.5 44 23444.5 45 23864.5 46 24512.0 47 31704.0 48 45739.0 49 44918.5 50 30655.0 51 21347.5 52 16749.5 53 14599.5 54 13035.5 55 11468.0 56 9945.5 57 8574.5 58 7604.0 59 6826.0 60 5863.5 61 4907.0 62 4007.0 63 2942.5 64 2085.5 65 1534.5 66 1096.0 67 859.0 68 666.5 69 481.0 70 366.0 71 309.5 72 259.5 73 208.0 74 177.0 75 158.5 76 126.0 77 85.5 78 62.5 79 45.5 80 28.5 81 19.0 82 15.0 83 9.5 84 5.5 85 1.5 86 0.5 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032161432010732724 2 0.0026254230212843046 3 0.0026254230212843046 4 3.9381345319264565E-4 5 0.0010501692085137218 6 0.0 7 0.0 8 0.0 9 0.002362880719155874 10-11 5.907201797889685E-4 12-13 5.907201797889685E-4 14-15 0.0019034316904311208 16-17 0.0022316095680916586 18-19 0.0 20-21 6.563557553210761E-5 22-23 0.0021003384170274436 24-25 9.84533632981614E-4 26-27 0.0060384729489539 28-29 0.006432286402146546 30-31 0.0013783470861742598 32-33 4.594490287247533E-4 34-35 0.010829869962797755 36-37 0.012470759351100447 38-39 0.009845336329816141 40-41 0.0019034316904311208 42-43 3.281778776605381E-4 44-45 8.532624819173989E-4 46-47 0.0 48-49 7.219913308531838E-4 50-51 6.563557553210762E-4 52-53 0.0017065249638347978 54-55 0.002494151870220089 56-57 7.876269063852913E-4 58-59 7.876269063852913E-4 60-61 0.0034786855032017034 62-63 0.0041350412585227795 64-65 0.008073175790449236 66-67 0.002494151870220089 68-69 0.002362880719155874 70-71 6.563557553210761E-5 72-73 9.188980574495065E-4 74-75 0.0051852104670365015 76-77 0.0021659739925595513 78-79 0.0027566941723485196 80-81 0.004331947985119103 82-83 0.0034786855032017034 84-85 0.005053939315972286 86-87 0.0034130499276695957 88-89 0.007810633488320805 90-91 0.011486225718118831 92-93 9.188980574495065E-4 94-95 0.0017065249638347978 96-97 7.876269063852913E-4 98-99 0.002822329747880627 100-101 0.0041350412585227795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 761782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.16181592990751 #Duplication Level Percentage of deduplicated Percentage of total 1 81.72475613371276 44.263611986307296 2 10.737580301772672 11.631336956744244 3 3.2612050538840336 5.298983635144534 4 1.3493746601915368 2.9233832786310203 5 0.7241649823620677 1.9611045238789513 6 0.4330455885875991 1.4072721275003999 7 0.28762312945596863 1.0904733696344693 8 0.19805524469764824 0.858162536581345 9 0.15201537665913184 0.7410085964214711 >10 0.9854631268568346 10.147500690786133 >50 0.08899997678143129 3.3072916717758027 >100 0.048501197511495106 4.814868712991595 >500 0.004122601788343143 1.5188810572317144 >1k 0.004122601788343143 5.35115298795203 >5k 7.275179626487899E-4 2.534839633648812 >10k+ 2.4250598754959666E-4 2.1501282347702495 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16370 2.1489087429212033 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7489 0.9830896503199078 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6749 0.8859489985323885 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5061 0.6643632955359933 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4755 0.6241943233103434 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4719 0.6194685618720317 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4012 0.5266598580696314 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3342 0.43870818685660723 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3007 0.39473235125009515 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2557 0.3356603332711983 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2516 0.3302782160775655 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2360 0.3097999165115479 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2239 0.2939161072327779 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1801 0.2364193430666516 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1458 0.1913933382516258 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1433 0.18811155947502042 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1396 0.18325452688564445 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1389 0.18233562882819496 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1349 0.17708478278562634 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1205 0.15818173703237934 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1203 0.15791919473025093 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 890 0.11683132444715155 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 867 0.1138120879726746 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 845 0.11092412264926187 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 844 0.11079285149819765 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.3127115106421522E-4 0.0 16-17 0.0 0.0 0.0 3.9381345319264565E-4 0.0 18-19 0.0 0.0 0.0 3.9381345319264565E-4 0.0 20-21 0.0 0.0 0.0 3.9381345319264565E-4 0.0 22-23 0.0 0.0 0.0 6.56355755321076E-4 0.0 24-25 0.0 0.0 0.0 0.0013783470861742598 0.0 26-27 0.0 0.0 0.0 0.002165973992559551 0.0 28-29 0.0 0.0 0.0 0.0041350412585227795 0.0 30-31 0.0 0.0 0.0 0.007285548884063945 0.0 32-33 0.0 0.0 0.0 0.011945674746843585 0.0 34-35 0.0 0.0 0.0 0.01791851212026538 0.0 36-37 0.0 0.0 0.0 0.025400967730925643 0.0 38-39 0.0 0.0 0.0 0.03826554053521874 0.0 40-41 0.0 0.0 0.0 0.0594658314320895 0.0 42-43 0.0 0.0 0.0 0.07895959736512545 0.0 44-45 0.0 0.0 0.0 0.10646090351307855 0.0 46-47 0.0 0.0 0.0 0.14617042671000366 0.0 48-49 0.0 1.3127115106421522E-4 0.0 0.18312325573458021 0.0 50-51 0.0 1.3127115106421522E-4 0.0 0.21587540792510193 0.0 52-53 0.0 1.3127115106421522E-4 0.0 0.2466584928496604 0.0 54-55 0.0 1.3127115106421522E-4 0.0 0.28531784683807176 0.0 56-57 0.0 1.3127115106421522E-4 0.0 0.34859054165102354 0.0 58-59 0.0 1.3127115106421522E-4 0.0 0.4105505249533331 0.0 60-61 0.0 1.3127115106421522E-4 0.0 0.4786802523556608 0.0 62-63 0.0 1.3127115106421522E-4 0.0 0.5486477758728875 0.0 64-65 0.0 1.3127115106421522E-4 0.0 0.6185496638145821 0.0 66-67 0.0 1.3127115106421522E-4 0.0 0.7011192178339736 0.0 68-69 0.0 1.3127115106421522E-4 0.0 0.8016729195491623 0.0 70-71 0.0 1.3127115106421522E-4 0.0 0.9173884392122681 0.0 72-73 0.0 1.3127115106421522E-4 0.0 1.027590570530677 0.0 74-75 0.0 1.3127115106421522E-4 0.0 1.148950749689544 0.0 76-77 0.0 1.3127115106421522E-4 0.0 1.2812064343867406 0.0 78-79 0.0 1.3127115106421522E-4 0.0 1.4449015597638168 0.0 80-81 0.0 1.3127115106421522E-4 0.0 1.6198203685568837 0.0 82-83 0.0 1.3127115106421522E-4 0.0 1.7936890081414367 0.0 84-85 0.0 1.3127115106421522E-4 0.0 1.9905957347377594 0.0 86-87 0.0 1.3127115106421522E-4 0.0 2.1839581402553487 0.0 88-89 0.0 1.3127115106421522E-4 0.0 2.3911696522102126 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGACCG 25 0.005020463 57.000473 5 TATCACG 60 0.003949962 31.66693 2 GCAACGC 35 0.0083866995 27.146646 84-85 GTAAGCG 135 4.0017767E-11 22.87056 94-95 CATGGGT 525 0.0 22.619236 4 GGGATCG 105 0.002195441 22.619236 9 AGTACGC 215 4.4161425E-8 22.093206 2 AGTACTT 7785 0.0 21.59941 12-13 CGGTATA 110 0.0028763467 21.591087 2 CGTATAG 155 3.8940758E-5 21.458834 1 GAGTACT 7755 0.0 20.8867 12-13 GTGTAGC 300 1.6552804E-10 20.590261 1 GTACTTT 8065 0.0 20.054413 14-15 GACATAT 335 3.45608E-11 19.857428 1 TATCGTG 120 0.004761822 19.79183 6 ATAGCCC 170 7.948856E-5 19.558987 8 GAGTACG 245 1.7478669E-7 19.394283 1 GTACGCA 250 2.1679443E-7 19.001404 3 TACGCAG 250 2.1694541E-7 19.000156 4 TACTTTT 8890 0.0 18.88795 14-15 >>END_MODULE