##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936676_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 711636 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.108472589919565 33.0 31.0 34.0 31.0 34.0 2 32.19767409181098 33.0 31.0 34.0 31.0 34.0 3 32.16543570027373 34.0 31.0 34.0 30.0 34.0 4 35.80291609755549 37.0 35.0 37.0 35.0 37.0 5 35.85598536330371 37.0 35.0 37.0 35.0 37.0 6 35.96052335744678 37.0 36.0 37.0 35.0 37.0 7 35.88568172492679 37.0 36.0 37.0 35.0 37.0 8 35.8931588621149 37.0 36.0 37.0 35.0 37.0 9 37.50938120050138 39.0 37.0 39.0 35.0 39.0 10-11 37.41710930869152 39.0 37.0 39.0 35.0 39.0 12-13 37.36060851334109 39.0 37.0 39.0 35.0 39.0 14-15 38.47018911353557 40.0 38.0 41.0 34.5 41.0 16-17 38.41646431602673 40.0 38.0 41.0 34.5 41.0 18-19 38.41002633368745 40.0 38.0 41.0 34.0 41.0 20-21 38.42076918537005 40.0 38.0 41.0 34.0 41.0 22-23 38.40707117121674 40.0 38.0 41.0 34.0 41.0 24-25 38.50492316296533 40.0 38.0 41.0 35.0 41.0 26-27 38.42837560213367 40.0 38.0 41.0 34.0 41.0 28-29 38.315471673720836 40.0 38.0 41.0 34.0 41.0 30-31 38.08904973891146 40.0 37.5 41.0 34.0 41.0 32-33 37.8312908565615 40.0 37.0 41.0 34.0 41.0 34-35 37.609046619339104 40.0 36.5 41.0 33.0 41.0 36-37 37.39297688706024 40.0 36.0 41.0 33.0 41.0 38-39 37.18384398765661 39.0 36.0 41.0 33.0 41.0 40-41 36.960802994789475 39.0 36.0 41.0 32.5 41.0 42-43 36.65336070687823 39.0 35.0 41.0 31.0 41.0 44-45 36.33864363241882 39.0 35.0 41.0 30.5 41.0 46-47 35.94829379064578 39.0 35.0 41.0 29.5 41.0 48-49 35.5886323907166 39.0 35.0 41.0 27.5 41.0 50-51 34.60616804096476 38.0 34.0 40.0 25.5 40.5 52-53 34.72286744908914 38.5 35.0 39.5 26.0 40.5 54-55 35.171092384308835 39.0 35.0 40.0 26.5 41.0 56-57 35.014777217566284 39.0 35.0 41.0 25.5 41.0 58-59 34.82453670134731 39.0 35.0 40.0 25.0 41.0 60-61 34.53832015243748 38.0 35.0 40.0 23.5 41.0 62-63 34.2361530332923 38.0 34.0 40.0 23.0 41.0 64-65 33.91713108948957 37.5 34.0 40.0 22.0 41.0 66-67 33.50082767032584 37.0 34.0 39.5 20.5 41.0 68-69 33.07919568431052 36.5 33.5 39.0 18.5 41.0 70-71 32.65078424925102 36.0 33.0 39.0 17.5 40.5 72-73 32.218285050222306 35.0 33.0 38.5 13.0 40.0 74-75 31.807374135091536 35.0 32.0 37.0 9.0 39.5 76-77 31.33185715731076 35.0 32.0 37.0 6.5 39.0 78-79 30.847119875891607 35.0 31.0 36.0 2.0 39.0 80-81 30.348957191597954 35.0 31.0 36.0 2.0 37.5 82-83 29.922423823415343 34.0 30.5 35.5 2.0 37.0 84-85 29.505930700526676 34.0 30.0 35.0 2.0 37.0 86-87 29.23740086223856 34.0 30.0 35.0 2.0 36.0 88-89 28.889913523205685 34.0 29.5 35.0 2.0 36.0 90-91 28.500161599469394 34.0 29.0 35.0 2.0 36.0 92-93 28.099044314790145 34.0 29.0 35.0 2.0 35.0 94-95 27.685278709902256 34.0 28.0 35.0 2.0 35.0 96-97 27.18624479368666 34.0 27.0 35.0 2.0 35.0 98-99 26.564270216796224 34.0 25.5 35.0 2.0 35.0 100-101 24.065171660792878 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5044759184648022 1101 2 0.44131582433439576 1101 3 0.5354822074517074 1101 4 0.4595505339330259 1101 5 0.4677108478319738 1101 6 0.45937619822554865 1101 7 0.44672232090625386 1101 8 0.47897452660754425 1101 9 0.6518101312859912 1101 10-11 0.6944504642178302 1101 12-13 0.6601101960678974 1101 14-15 0.9885691933230802 1101 16-17 0.9320063265584295 1101 18-19 0.9799068402592042 1101 20-21 0.8672146590595204 1101 22-23 0.7954252032601303 1101 24-25 0.8107275014815158 1101 26-27 0.7936678107171531 1101 28-29 0.8692439164892605 1101 30-31 0.8676200408450825 1101 32-33 0.8177637791898746 1101 34-35 0.699342517354296 1101 36-37 0.8670929498567403 1101 38-39 0.8776029500186411 1101 40-41 1.0183328408658952 1101 42-43 1.0813011917795237 1101 44-45 1.1824943822096259 1101 46-47 1.2977713816242797 1101 48-49 1.2102063397541016 1101 50-51 0.8971030312491663 1101 52-53 0.8784425663902127 1101 54-55 0.9875242708855936 1101 56-57 0.9938919664018258 1101 58-59 1.0639124524361918 1101 60-61 1.100644023734965 1101 62-63 1.0827705830250025 1101 64-65 1.0482778197510854 1101 66-67 1.0991176238372091 1101 68-69 1.1485323474211881 1101 70-71 1.2153070520212026 1101 72-73 1.1209605314670696 1101 74-75 0.9345984384001298 1101 76-77 0.9177505092169156 1101 78-79 0.9177617259755344 1101 80-81 1.1922897079872214 1101 82-83 1.190823919551292 1101 84-85 1.2765374076785427 1101 86-87 1.2191856191790116 1101 88-89 1.3158776220764388 1101 90-91 1.2194488638792187 1101 92-93 1.3254914217690548 1101 94-95 1.4148251650588577 1101 96-97 1.3292211202881923 1101 98-99 1.34902725530549 1101 100-101 1.6800251745365529 1102 1 0.22851591285335004 1102 2 0.28724177860102174 1102 3 0.4039266859049846 1102 4 0.29237118409690765 1102 5 0.2654637141389955 1102 6 0.2841150741485734 1102 7 0.44200997187358837 1102 8 0.26317189687446785 1102 9 0.40691719534588344 1102 10-11 0.4941915719705676 1102 12-13 0.4040708648516187 1102 14-15 0.5306315315154322 1102 16-17 0.5413506787539077 1102 18-19 0.48536972174266424 1102 20-21 0.5194605174212299 1102 22-23 0.4985115049269879 1102 24-25 0.5588457501966175 1102 26-27 0.51307707727414 1102 28-29 0.2952460080325423 1102 30-31 0.30008678658228405 1102 32-33 0.2956986352687352 1102 34-35 0.18388677772592388 1102 36-37 0.2924388834629923 1102 38-39 0.1733790528613497 1102 40-41 0.3790934484337143 1102 42-43 0.28096131599678387 1102 44-45 0.4264295443680055 1102 46-47 0.5098494063912113 1102 48-49 0.36804325039164354 1102 50-51 0.4672405119597869 1102 52-53 0.3152779593835433 1102 54-55 0.4005791994032748 1102 56-57 0.4765608521448996 1102 58-59 0.416915219905043 1102 60-61 0.5284258400617503 1102 62-63 0.5995061175801055 1102 64-65 0.5591908294053454 1102 66-67 0.5026566681039597 1102 68-69 0.7195096337713167 1102 70-71 0.38997132106508303 1102 72-73 0.6844981728694322 1102 74-75 0.4338874412186158 1102 76-77 0.4875417374884634 1102 78-79 0.4943326152640246 1102 80-81 0.6743151891059433 1102 82-83 0.5813603238823184 1102 84-85 0.7323274116320739 1102 86-87 0.6465472138532284 1102 88-89 0.8456699981461249 1102 90-91 1.0454981426793815 1102 92-93 0.6626131266318893 1102 94-95 0.7545791536573958 1102 96-97 0.7215487273841354 1102 98-99 0.9700689458846199 1102 100-101 1.4908865332744838 1103 1 0.31265101481782054 1103 2 0.44816987436742295 1103 3 0.27694959492228577 1103 4 0.4600994735689028 1103 5 0.41068910187046015 1103 6 0.477679743888217 1103 7 0.41346530044285856 1103 8 0.3765960019469432 1103 9 0.4553295012879772 1103 10-11 0.24407306821816377 1103 12-13 0.3342343615962875 1103 14-15 0.5679461211849741 1103 16-17 0.6164372185212059 1103 18-19 0.5583853283665761 1103 20-21 0.6605638962514604 1103 22-23 0.5573572655673402 1103 24-25 0.43520982447093104 1103 26-27 0.4780191833868841 1103 28-29 0.42117193870335967 1103 30-31 0.5456425755866761 1103 32-33 0.6187840630224457 1103 34-35 0.5316639035551347 1103 36-37 0.2173472192091026 1103 38-39 0.34328909249924777 1103 40-41 0.328167581818974 1103 42-43 0.5766525195593317 1103 44-45 0.6580832418131877 1103 46-47 0.9100250481690964 1103 48-49 0.9975230125129784 1103 50-51 0.8703524845540258 1103 52-53 0.7227732896951409 1103 54-55 0.8106041044081067 1103 56-57 0.9478323397577384 1103 58-59 0.8027980280212077 1103 60-61 0.8956965128812158 1103 62-63 0.8219184440201488 1103 64-65 0.8846277559432707 1103 66-67 0.916209141565524 1103 68-69 0.9560879628299759 1103 70-71 0.8713681536199189 1103 72-73 1.041871540400166 1103 74-75 0.9788887607739127 1103 76-77 1.0478979405513655 1103 78-79 0.9071292269534936 1103 80-81 1.0302030960862645 1103 82-83 0.8627183384512342 1103 84-85 1.1672073577224715 1103 86-87 1.116382564099073 1103 88-89 0.9570987562524209 1103 90-91 1.0713005732233754 1103 92-93 0.921678343410484 1103 94-95 0.9130225139882313 1103 96-97 0.8968612570100767 1103 98-99 1.002861816698875 1103 100-101 1.5214637041991743 1104 1 0.13778754741187882 1104 2 0.03648429641138051 1104 3 0.18916438946600778 1104 4 0.20709826714008273 1104 5 0.2883918393935687 1104 6 0.17219954425265627 1104 7 0.2832225215073976 1104 8 0.31858963549121455 1104 9 0.3367387370093766 1104 10-11 0.36693456411062186 1104 12-13 0.4734070923406648 1104 14-15 0.5893281891334752 1104 16-17 0.5427670981822459 1104 18-19 0.6710093573102967 1104 20-21 0.5445888229473681 1104 22-23 0.45107590733726255 1104 24-25 0.5177682728422468 1104 26-27 0.2816633119898171 1104 28-29 0.18253357796660907 1104 30-31 0.2025397675013494 1104 32-33 0.3501184420155141 1104 34-35 0.3664419563287993 1104 36-37 0.25385787819938344 1104 38-39 -0.00132406404975427 1104 40-41 0.2639890039250332 1104 42-43 0.3462946475782118 1104 44-45 0.5628315236761949 1104 46-47 0.6924985311806537 1104 48-49 0.5280979888270636 1104 50-51 0.5389109408536896 1104 52-53 0.33369272588456766 1104 54-55 0.444295710544111 1104 56-57 0.4947529070567782 1104 58-59 0.5257614030620061 1104 60-61 0.4302343370016004 1104 62-63 0.4605124060619019 1104 64-65 0.6316397634117621 1104 66-67 0.7918703319526728 1104 68-69 0.7934301723785993 1104 70-71 0.6593511711190843 1104 72-73 0.7924935846165155 1104 74-75 0.8360868331305653 1104 76-77 0.8668649044866825 1104 78-79 0.7799421617733024 1104 80-81 0.8233269803378072 1104 82-83 0.658348238329534 1104 84-85 0.6827323656838473 1104 86-87 0.4471231105465847 1104 88-89 0.5530171155092205 1104 90-91 0.4978293220390775 1104 92-93 0.5979761793705194 1104 94-95 0.543960343922727 1104 96-97 0.5356719269678827 1104 98-99 0.6292177277376325 1104 100-101 1.2929445460301459 1105 1 0.24103669521639048 1105 2 0.3209328614466145 1105 3 0.23836796136760796 1105 4 0.3805627467954409 1105 5 0.32704979705217596 1105 6 0.24269092470479592 1105 7 0.1733774851540275 1105 8 0.3005990741226796 1105 9 0.32337547517448684 1105 10-11 0.4153713343419909 1105 12-13 0.4272268102970642 1105 14-15 0.4677768796453492 1105 16-17 0.5769706352292232 1105 18-19 0.4188222298190638 1105 20-21 0.42890145495924514 1105 22-23 0.46940943818011505 1105 24-25 0.3746335876143334 1105 26-27 0.3789007346996982 1105 28-29 0.47595929928299796 1105 30-31 0.27088131490712897 1105 32-33 0.19978279201168903 1105 34-35 0.24986858386534294 1105 36-37 0.28706317889019317 1105 38-39 -0.013871727744486861 1105 40-41 0.05103354116022274 1105 42-43 -0.10905030860953246 1105 44-45 -0.10891601733027301 1105 46-47 0.21985940895943656 1105 48-49 0.08544569155896653 1105 50-51 0.023342078578245662 1105 52-53 0.14633728542671776 1105 54-55 0.048801715995374195 1105 56-57 0.11816545332628436 1105 58-59 0.10640035798103753 1105 60-61 -0.02119365683610397 1105 62-63 0.25057395932325477 1105 64-65 0.23037802611801794 1105 66-67 0.11794437711032657 1105 68-69 0.23919847279756112 1105 70-71 0.2798254728067491 1105 72-73 0.06680781994096918 1105 74-75 0.24118188168305466 1105 76-77 0.32223983564880143 1105 78-79 0.35238400683753923 1105 80-81 0.6243510091137807 1105 82-83 0.4216470191148787 1105 84-85 0.40707971289861433 1105 86-87 0.4771273628103927 1105 88-89 0.4412641371818218 1105 90-91 0.4631817371157929 1105 92-93 0.6189147446148873 1105 94-95 0.690751278932801 1105 96-97 0.7602235222639031 1105 98-99 1.2392704976343936 1105 100-101 1.8906141660450615 1106 1 0.21219200066571275 1106 2 0.02330478009363901 1106 3 0.2395234922130669 1106 4 0.36625009230517236 1106 5 0.26043922897878957 1106 6 0.1253226499619302 1106 7 0.309222232297131 1106 8 0.39327745637949363 1106 9 0.3780490640068592 1106 10-11 0.4764897499574374 1106 12-13 0.45982735575570644 1106 14-15 0.45090748167842065 1106 16-17 0.38512997526934356 1106 18-19 0.41349536785916996 1106 20-21 0.20434755147386596 1106 22-23 0.15711558172740325 1106 24-25 0.013633880629306816 1106 26-27 0.11562302412335868 1106 28-29 0.07497657214004505 1106 30-31 0.18497608646814 1106 32-33 0.39539883721112545 1106 34-35 0.5099486746248516 1106 36-37 0.309537427381926 1106 38-39 0.47161651784714564 1106 40-41 0.41760128396821017 1106 42-43 0.5454159157798344 1106 44-45 0.39185859404922496 1106 46-47 0.6236190164696396 1106 48-49 0.7494431671222728 1106 50-51 0.4968783185199399 1106 52-53 0.5206233431732059 1106 54-55 0.8249563076902788 1106 56-57 0.8417869681385639 1106 58-59 0.6988939154082132 1106 60-61 0.82926051637331 1106 62-63 0.7611442866735771 1106 64-65 0.5333900697847653 1106 66-67 0.6466305430469603 1106 68-69 0.5488371627952304 1106 70-71 0.7489033772237548 1106 72-73 0.6210824576166871 1106 74-75 0.7099246971812931 1106 76-77 0.5608088707279535 1106 78-79 0.6340991211509426 1106 80-81 0.7173051259741641 1106 82-83 0.6351873161869825 1106 84-85 0.8542851765008912 1106 86-87 0.9870290149640546 1106 88-89 1.0607308499294774 1106 90-91 1.350050909686587 1106 92-93 1.3572290820629505 1106 94-95 1.2266959516951843 1106 96-97 1.3198122494519389 1106 98-99 1.417561093356376 1106 100-101 2.036062291124889 1107 1 0.46847787584725253 1107 2 0.42908663264437763 1107 3 0.34999352912971915 1107 4 0.5223736132515029 1107 5 0.46047742997530605 1107 6 0.4693588486993221 1107 7 0.5734183483096089 1107 8 0.5127527195876596 1107 9 0.5152515655134948 1107 10-11 0.581344899023648 1107 12-13 0.5946958093069199 1107 14-15 0.6291604117936558 1107 16-17 0.6345931854044622 1107 18-19 0.8167389947144699 1107 20-21 0.5372288945682158 1107 22-23 0.6504921546369715 1107 24-25 0.5063567554518897 1107 26-27 0.4415903161666819 1107 28-29 0.5833437429940034 1107 30-31 0.4491672626965908 1107 32-33 0.4071538766141316 1107 34-35 0.3664495950712805 1107 36-37 0.39288146358066456 1107 38-39 0.5539964728621811 1107 40-41 0.5905513559243971 1107 42-43 0.3246594372530822 1107 44-45 0.6233525164682732 1107 46-47 0.6378777030798588 1107 48-49 0.5891422354790024 1107 50-51 0.5841865314048746 1107 52-53 0.7243120445698494 1107 54-55 0.543429782696613 1107 56-57 0.6660837898815117 1107 58-59 0.6741019626457287 1107 60-61 0.789873971686383 1107 62-63 0.9636038051434284 1107 64-65 0.74804426843653 1107 66-67 0.5074142562496817 1107 68-69 0.5376888935308912 1107 70-71 0.7591880075797164 1107 72-73 0.6646546459739469 1107 74-75 0.6832638594597853 1107 76-77 0.4830937184344144 1107 78-79 0.5161313153700782 1107 80-81 0.4486245093573906 1107 82-83 0.49741673951659493 1107 84-85 0.5762971834692827 1107 86-87 0.8582776606399314 1107 88-89 1.0550986728349265 1107 90-91 0.6988474136367664 1107 92-93 1.212172323944415 1107 94-95 1.3699299295056733 1107 96-97 1.5869928183174657 1107 98-99 1.433828625473204 1107 100-101 1.8945275990216253 1108 1 0.1844116054749243 1108 2 0.199168485061044 1108 3 0.3145760195595244 1108 4 0.2767229954680559 1108 5 0.20296910468197638 1108 6 0.25311908025140895 1108 7 0.5403557815257116 1108 8 0.5321265330388414 1108 9 0.24056875659583454 1108 10-11 0.31974913999550125 1108 12-13 0.2578793392312946 1108 14-15 0.5568548501864825 1108 16-17 0.587123452125077 1108 18-19 0.576936830656912 1108 20-21 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2316 64-65 -3.6785772642937147 2316 66-67 -3.191591885352345 2316 68-69 -3.481720978622185 2316 70-71 -3.009818068893974 2316 72-73 -2.4854336029205015 2316 74-75 -2.869042334896193 2316 76-77 -3.486675336488311 2316 78-79 -3.582862126206546 2316 80-81 -4.092935509660084 2316 82-83 -3.1785372277759585 2316 84-85 -3.1950079232434945 2316 86-87 -4.140922889043221 2316 88-89 -5.260781894823648 2316 90-91 -4.207341732074855 2316 92-93 -3.306018020128576 2316 94-95 -3.2914287778271287 2316 96-97 -4.41839492322725 2316 98-99 -6.218208588857635 2316 100-101 -7.456364333531468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1587.0 3 434.0 4 341.0 5 308.0 6 337.0 7 412.0 8 485.0 9 583.0 10 782.0 11 1247.0 12 2107.0 13 3050.0 14 3501.0 15 5102.0 16 7696.0 17 11998.0 18 8244.0 19 4140.0 20 3477.0 21 3576.0 22 3647.0 23 4070.0 24 4431.0 25 5074.0 26 5750.0 27 6887.0 28 8254.0 29 10180.0 30 12888.0 31 16847.0 32 22541.0 33 30981.0 34 45564.0 35 71405.0 36 104943.0 37 151911.0 38 128640.0 39 18211.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.264998882175696 19.453236277124816 14.861579605260383 21.420185235439106 2 14.150036404321293 20.945275449993954 40.40415824494627 24.50052990073849 3 19.69182726699075 27.357497515982736 31.11816614175552 21.83250907527099 4 10.929715158695693 16.91058144617361 40.3934399503035 31.766263444827192 5 11.150574071289126 38.9948235902611 35.393824569885744 14.460777768564029 6 27.18626048187826 36.91434306066629 19.30886367022458 16.59053278723087 7 24.28616425604437 35.78248434243867 22.099730299033407 17.831621102483556 8 24.803948859402723 31.46364231949096 21.617362159669558 22.115046661436764 9 25.4845141079935 17.035887226037218 23.70618142142885 33.773417244540425 10-11 23.291747329174733 26.542991183710885 27.60105834834113 22.564203138773255 12-13 26.021647455721116 25.517641270733765 28.2689063817824 20.19180489176272 14-15 23.15416902113256 25.294905638629544 29.930111018338128 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27.887165802078524 33.89550884929326 19.3775949062022 42-43 18.418645239620922 27.72756399446899 32.21286803143233 21.64092273447776 44-45 18.454091888591527 27.566928763190603 31.8996365404383 22.079342807779568 46-47 19.715893282220918 27.785209812909446 31.91120716548113 20.587689739388505 48-49 19.60303921127774 27.495461137216985 31.347803359039506 21.55369629246577 50-51 18.622172414641085 28.231202419805307 32.420753835398436 20.725871330155172 52-53 19.14197587246271 29.17668467683574 30.351584730936505 21.32975471976505 54-55 18.520800691365658 27.99496933118804 31.631875891433108 21.852354086013197 56-57 20.029032471118594 27.95077970312682 29.938154934466155 22.08203289128843 58-59 20.41543472884252 27.890744446443026 30.45638378001619 21.237437044698265 60-61 20.278709557743735 27.84207723148225 31.824013738035784 20.055199472738234 62-63 19.543478902393268 28.554525512878342 31.44791960889294 20.45407597583545 64-65 18.819813021599614 29.536362141721927 30.991325584957412 20.65249925172104 66-67 19.101054124068074 28.916078290299712 29.631193584900096 22.351674000732118 68-69 18.718443081964725 28.656386705658004 30.75591861283763 21.869251599539634 70-71 20.17090606068697 28.335176548124767 29.702551505791725 21.791365885396537 72-73 19.500913562895292 27.88917779339424 31.42874209416725 21.181166549543217 74-75 18.970009739561593 29.621478192798346 30.454962608797086 20.953549458842975 76-77 18.644549016962113 28.837558400377812 30.527936604316707 21.98995597834337 78-79 19.015859139806103 28.36852120970213 31.062143541799596 21.55347610869217 80-81 20.029049095360296 28.755301776112564 29.580397271324454 21.635251857202686 82-83 19.287736876497494 28.340160622817436 31.118035989066968 21.254066511618102 84-85 19.508736823097337 28.64788449364087 30.10365865226186 21.739720030999933 86-87 19.03129162989652 29.466589285413164 30.642976386539033 20.85914269815128 88-89 19.04627069639664 28.987215924061395 29.393678574816178 22.572834804725787 90-91 18.484172243317154 28.8309551179177 29.678554464739925 23.00631817402522 92-93 19.52353380964962 29.15867965574812 30.2700218670774 21.04776466752486 94-95 19.1682957095858 28.285829926513884 30.288041958336787 22.257832405563526 96-97 18.15832318549223 28.538282247473347 31.44540156992313 21.857992997111293 98-99 18.669842175612487 29.09863426879198 29.617828575926193 22.61369497966934 100-101 18.37152462523155 28.045722734593948 30.87675999921294 22.70599264096157 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1033.0 1 764.0 2 792.0 3 1019.0 4 757.0 5 640.0 6 1105.0 7 1973.5 8 2926.0 9 3249.0 10 2580.0 11 1956.5 12 1900.5 13 1943.5 14 1956.0 15 1938.0 16 1993.0 17 2029.0 18 2095.5 19 2241.5 20 2379.5 21 2678.5 22 3043.5 23 3435.5 24 3817.5 25 4468.0 26 5536.0 27 6775.5 28 7623.0 29 8830.5 30 10656.5 31 12535.5 32 15108.0 33 17595.5 34 19991.0 35 21631.0 36 23554.5 37 25520.5 38 24835.0 39 23421.5 40 22385.5 41 22051.0 42 21914.0 43 22185.0 44 22786.5 45 23037.5 46 23336.0 47 29306.5 48 46098.0 49 44438.0 50 26197.5 51 18306.0 52 15264.5 53 13749.5 54 12545.5 55 11362.5 56 10001.0 57 8637.5 58 7818.5 59 6925.0 60 5826.0 61 4882.5 62 3990.5 63 2870.0 64 1849.5 65 1410.5 66 1089.5 67 800.5 68 589.0 69 410.5 70 306.0 71 253.0 72 243.5 73 245.5 74 171.0 75 137.5 76 127.0 77 85.0 78 59.0 79 41.5 80 28.0 81 23.5 82 19.0 83 10.0 84 4.0 85 2.0 86 2.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06084571325790151 2 0.025574872547201095 3 0.012225351162673053 4 0.016440989494629278 5 0.01953245760473051 6 0.023888617214418605 7 0.06716917075583584 8 0.048058276984300964 9 0.05733268131460466 10-11 0.04763671315110534 12-13 0.0331630215447223 14-15 0.031195723656476063 16-17 0.041313255653171004 18-19 0.03702735668234884 20-21 0.015668122467103966 22-23 0.012295611801538988 24-25 0.01897037249380301 26-27 0.021710537409574557 28-29 0.00505876599834747 30-31 4.918244720615596E-4 32-33 0.008220494747314639 34-35 0.009906750080097127 36-37 0.0036535532210287282 38-39 0.008361016025046512 40-41 0.003583292582162791 42-43 0.00168625533278249 44-45 3.513031943296854E-4 46-47 2.8104255546374836E-4 48-49 5.620851109274967E-4 50-51 0.0011944308607209303 52-53 1.4052127773187418E-4 54-55 1.4052127773187418E-4 56-57 0.002669904276905609 58-59 0.002810425554637483 60-61 0.004918244720615595 62-63 0.0021078191659781127 64-65 4.215638331956225E-4 66-67 3.513031943296854E-4 68-69 0.0035130319432968536 70-71 0.0023888617214418606 72-73 0.019110893771534885 74-75 0.014754734161846788 76-77 0.02360757465895486 78-79 0.017143595883288648 80-81 0.011522744774013681 82-83 0.004356159609688099 84-85 0.0030212074712352944 86-87 0.0011241702218549934 88-89 0.0011944308607209303 90-91 0.005339808553811218 92-93 0.008993361774839947 94-95 0.010258053274426812 96-97 0.010187792635560878 98-99 0.02093767038204925 100-101 0.018267766105143638 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 711636.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.37267020731051 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2267720771264 42.81917047869535 2 11.6649067208603 12.451744388230336 3 3.571781127168214 5.719064884591347 4 1.4667474144063217 3.1313690410613613 5 0.744115653470738 1.9857719684395525 6 0.45181102547793545 1.446861651531637 7 0.2995384874565257 1.1191018233789638 8 0.2149714567825191 0.9178880533470798 9 0.16471609620353672 0.7912204092456299 >10 1.041515560735932 10.59390886581507 >50 0.09351718403304574 3.4021166987417395 >100 0.04980512400172333 4.5665040139771715 >500 0.004503654829930567 1.8562380695872742 >1k 0.003973813085232853 4.251475188631182 >5k 0.0013246043617442845 4.947564464726409 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9643 1.3550466811684625 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7608 1.0690858809840986 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6310 0.886689262488126 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6080 0.854369368609795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5350 0.7517888358655267 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3805 0.5346834617697812 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3655 0.51360527011 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3532 0.49632115294897955 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3355 0.4714488867904378 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1962 0.27570274690993707 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1948 0.2737354490216909 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1642 0.2307359380357374 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1575 0.2213210124277018 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1334 0.18745538449432012 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1314 0.18464495893968264 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1292 0.18155349082958142 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1272 0.17874306527494394 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1199 0.16848501200051713 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1109 0.15583809700464843 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1074 0.15091985228403285 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1000 0.14052127773187414 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 991 0.1392565862322873 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 897 0.1260475861254911 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 869 0.12211299034899864 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 858 0.12056725629394803 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 851 0.11958360734982491 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 845 0.11874047968343365 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 803 0.11283858601869494 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 746 0.10482887318797812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.810425554637483E-4 0.0 24-25 0.0 0.0 0.0 6.323457497934337E-4 0.0 26-27 0.0 0.0 0.0 0.0013349521384528045 0.0 28-29 0.0 0.0 0.0 0.003653553221028728 0.0 30-31 0.0 0.0 0.0 0.007026063886593708 0.0 32-33 0.0 0.0 0.0 0.010820138385354311 0.0 34-35 0.0 0.0 0.0 0.0163004682168974 0.0 36-37 0.0 0.0 0.0 0.02480200551967579 0.0 38-39 0.0 0.0 0.0 0.03660579284915322 0.0 40-41 0.0 0.0 0.0 0.055505904704090295 0.0 42-43 0.0 0.0 0.0 0.07264950058737894 0.0 44-45 0.0 0.0 0.0 0.09597603269087004 0.0 46-47 0.0 0.0 0.0 0.13307365001208482 0.0 48-49 0.0 0.0 0.0 0.16342624600216965 0.0 50-51 0.0 0.0 0.0 0.196519006908026 0.0 52-53 0.0 0.0 0.0 0.2260284752317196 0.0 54-55 0.0 0.0 0.0 0.2594725393319056 0.0 56-57 0.0 0.0 0.0 0.32158294408939403 0.0 58-59 0.0 0.0 0.0 0.38095318393111083 0.0 60-61 0.0 0.0 0.0 0.4396910780230342 0.0 62-63 0.0 0.0 0.0 0.4992720997813489 0.0 64-65 0.0 0.0 0.0 0.5631390205104857 0.0 66-67 0.0 0.0 0.0 0.6425335424289946 0.0 68-69 0.0 0.0 0.0 0.7360504527595568 0.0 70-71 0.0 0.0 0.0 0.8425655812803174 0.0 72-73 0.0 0.0 0.0 0.9473944544682956 0.0 74-75 0.0 0.0 0.0 1.0656431096796677 0.0 76-77 0.0 0.0 0.0 1.1936579936934049 0.0 78-79 0.0 0.0 0.0 1.353571207752278 0.0 80-81 0.0 0.0 0.0 1.5299956719446457 0.0 82-83 0.0 0.0 0.0 1.7053662265540248 0.0 84-85 0.0 0.0 0.0 1.9034309675171013 0.0 86-87 0.0 0.0 0.0 2.1017767510356418 0.0 88-89 0.0 0.0 0.0 2.326189231573445 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACGAC 65 0.0058268313 29.246933 3 TACGTGG 70 0.008366861 27.157867 2 CATTCCG 105 6.879558E-5 27.155956 9 GCGGTAT 110 9.440626E-5 25.921597 1 GTACATG 5980 0.0 25.827677 1 TACATGG 5930 0.0 25.806845 2 ACATGGG 5935 0.0 24.74409 3 GTATCAA 10295 0.0 24.142292 1 GAGTACT 5350 0.0 23.493326 12-13 CATGGGG 1560 0.0 22.850775 4 CATGGGA 4010 0.0 22.638792 4 CCTATAC 235 4.611138E-9 22.24634 3 GTACTTT 5730 0.0 21.931452 14-15 CATGGGC 770 0.0 21.604368 4 AGTACTT 5510 0.0 21.301882 12-13 ATCAACG 11675 0.0 21.12731 3 CTCCCGC 135 3.778047E-4 21.124273 7 TCAACGC 11710 0.0 21.065643 4 TATCAAC 11745 0.0 21.00139 2 CAACGCA 11780 0.0 20.900118 5 >>END_MODULE