##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936676_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 711636 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.82882400553092 31.0 31.0 31.0 28.0 34.0 2 30.928124771652925 31.0 31.0 33.0 28.0 34.0 3 30.901604190906586 31.0 31.0 34.0 27.0 34.0 4 34.720102692949766 35.0 35.0 37.0 32.0 37.0 5 34.52082525335987 35.0 35.0 37.0 30.0 37.0 6 34.76810054578464 35.0 35.0 37.0 32.0 37.0 7 34.69759961553378 35.0 35.0 37.0 31.0 37.0 8 34.69040211568836 35.0 35.0 37.0 31.0 37.0 9 36.03252927058215 37.0 35.0 39.0 31.0 39.0 10-11 35.85639709064746 37.0 35.0 39.0 30.0 39.0 12-13 35.72243956179845 37.0 35.0 39.0 30.0 39.0 14-15 36.288663024355145 38.0 34.5 40.0 30.0 41.0 16-17 36.206629512840834 38.0 34.5 40.0 30.0 41.0 18-19 36.5533987881445 38.0 35.0 40.0 30.0 41.0 20-21 36.63061171722622 38.0 35.0 40.0 30.0 41.0 22-23 36.47112779567082 38.0 35.0 40.0 30.0 41.0 24-25 36.20704967146125 38.0 34.5 40.0 29.5 41.0 26-27 35.767471010460405 38.0 34.0 40.0 28.0 41.0 28-29 35.5593280834584 38.0 34.0 40.0 27.0 41.0 30-31 35.35259528747843 38.0 34.0 40.0 27.0 41.0 32-33 34.98022303537202 37.0 33.5 40.0 27.0 41.0 34-35 34.578312508079975 37.0 33.0 40.0 25.5 41.0 36-37 34.15329606709048 37.0 33.0 39.5 24.5 41.0 38-39 33.81211391778943 36.5 33.0 39.0 24.0 40.0 40-41 33.513878583995194 36.0 32.5 39.0 23.5 40.0 42-43 32.968396062031715 36.0 31.0 39.0 20.0 40.0 44-45 32.36337326948046 36.0 31.0 39.0 16.5 40.0 46-47 31.56450488733004 35.5 30.0 39.0 8.5 40.0 48-49 30.805172869275864 35.0 29.0 39.0 4.5 40.0 50-51 30.280006211040476 35.0 28.0 38.5 2.0 40.0 52-53 29.87616281357323 35.0 27.0 38.0 2.0 40.0 54-55 29.572225828935018 34.5 27.0 38.0 2.0 40.0 56-57 29.08776467182661 34.0 26.0 38.0 2.0 40.0 58-59 28.587603774963604 34.0 25.0 38.0 2.0 40.0 60-61 27.896539101450742 33.0 23.0 37.5 2.0 39.5 62-63 27.46661565744285 33.0 22.5 37.0 2.0 39.0 64-65 26.920609693719822 32.0 22.0 36.5 2.0 39.0 66-67 26.31359360684395 31.5 20.5 36.0 2.0 39.0 68-69 25.769355400794787 31.0 19.5 35.5 2.0 38.0 70-71 25.178616596124986 31.0 17.5 35.0 2.0 38.0 72-73 24.520490110112476 30.5 13.5 34.5 2.0 37.5 74-75 23.887889314199956 29.5 9.5 34.0 2.0 36.5 76-77 23.016699548645654 28.5 8.0 33.5 2.0 35.5 78-79 23.593278024158415 30.0 7.5 34.0 2.0 36.0 80-81 23.194003676036623 30.0 4.5 34.0 2.0 36.0 82-83 22.61919787644245 29.0 2.0 34.0 2.0 35.0 84-85 22.011696991158402 29.0 2.0 34.0 2.0 35.0 86-87 21.430371706883854 29.0 2.0 34.0 2.0 35.0 88-89 20.598931195161573 27.5 2.0 33.0 2.0 35.0 90-91 19.721304149874374 26.0 2.0 33.0 2.0 34.5 92-93 18.735685800043843 25.0 2.0 32.0 2.0 34.0 94-95 17.76969475968051 23.5 2.0 32.0 2.0 34.0 96-97 16.57315818761277 18.0 2.0 31.5 2.0 34.0 98-99 15.326601661523586 2.0 2.0 31.0 2.0 34.0 100-101 12.639685176129369 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24587548864663233 1101 2 0.5074029121863113 1101 3 0.3550222867397501 1101 4 0.4087714073953137 1101 5 0.1541402320240408 1101 6 0.3098063250534082 1101 7 0.39193945667400953 1101 8 0.2427896423005933 1101 9 0.23046222413316286 1101 10-11 0.39921689516733494 1101 12-13 0.49272562231478645 1101 14-15 0.7829211001893341 1101 16-17 0.5744202256347393 1101 18-19 0.5837026200201834 1101 20-21 0.44473296845796995 1101 22-23 0.7560398086492057 1101 24-25 0.4977264263473984 1101 26-27 0.6775401440208597 1101 28-29 0.8681507928111003 1101 30-31 0.9490376245492342 1101 32-33 0.6935795393706812 1101 34-35 1.025411676342479 1101 36-37 0.959886283347096 1101 38-39 0.9504520873020184 1101 40-41 1.1512277071916301 1101 42-43 1.0344434190289533 1101 44-45 1.0959529481801198 1101 46-47 1.2390614126016217 1101 48-49 1.1087788859034582 1101 50-51 1.251775668077677 1101 52-53 1.4898517535142481 1101 54-55 1.3638506686683591 1101 56-57 1.6905285715732354 1101 58-59 1.846064869145529 1101 60-61 1.7281830754822813 1101 62-63 1.6518037742645646 1101 64-65 1.6747097733879706 1101 66-67 1.238922154134869 1101 68-69 1.2409107613147334 1101 70-71 1.5901510057689272 1101 72-73 1.9375031992978613 1101 74-75 1.793094466534825 1101 76-77 1.747087366192158 1101 78-79 1.8399401309985208 1101 80-81 1.5625169385639772 1101 82-83 1.7359362266790441 1101 84-85 1.900229806538384 1101 86-87 1.9618434363675803 1101 88-89 1.885715243505505 1101 90-91 1.8244024910211074 1101 92-93 1.8790213529784516 1101 94-95 1.9231421248221423 1101 96-97 2.0606536626191634 1101 98-99 2.2431092929242737 1101 100-101 2.5341763877781087 1102 1 0.35418682251560796 1102 2 0.3478513186598917 1102 3 0.30175169970477 1102 4 0.3443569757466918 1102 5 0.43562752334796073 1102 6 0.3436587182125308 1102 7 0.19327727917340098 1102 8 0.3298412039437295 1102 9 0.6873624039548716 1102 10-11 0.6202203258084396 1102 12-13 0.6122273503886504 1102 14-15 0.7405928554277565 1102 16-17 0.7688827372721363 1102 18-19 0.6925483176901537 1102 20-21 0.5553590285770866 1102 22-23 0.5989897774035882 1102 24-25 0.5839286173665172 1102 26-27 0.7945103395291397 1102 28-29 0.8593229497788641 1102 30-31 1.1209350624087193 1102 32-33 1.0829043789977746 1102 34-35 1.0248696605538754 1102 36-37 0.9700634268601149 1102 38-39 1.17662634856638 1102 40-41 0.9746051669868123 1102 42-43 1.3806148745645217 1102 44-45 1.1672988052664905 1102 46-47 0.9601573047596048 1102 48-49 1.2118345796199002 1102 50-51 0.9167467818244717 1102 52-53 0.8169627455944415 1102 54-55 0.6818730124721633 1102 56-57 0.7372936938137364 1102 58-59 0.890936762119729 1102 60-61 1.3682686502103216 1102 62-63 1.2676491275950923 1102 64-65 1.0321073324039247 1102 66-67 0.6708647387212174 1102 68-69 1.1214753504667634 1102 70-71 0.8552993905618784 1102 72-73 0.9473564086689983 1102 74-75 1.1768441955269324 1102 76-77 1.0477964503767758 1102 78-79 1.1901327060198845 1102 80-81 1.441123256555077 1102 82-83 1.6990925440324744 1102 84-85 1.671445579835499 1102 86-87 1.4458424926224431 1102 88-89 1.3949722355091758 1102 90-91 1.3561250809638459 1102 92-93 1.498115612712418 1102 94-95 1.2622571724557616 1102 96-97 1.3981356485650132 1102 98-99 1.791707703861512 1102 100-101 1.9319311670820323 1103 1 0.23241877215215112 1103 2 0.24571261418134682 1103 3 0.32527309315516106 1103 4 0.2603999496912408 1103 5 0.3203252939400727 1103 6 0.2882707048991122 1103 7 0.23990626378201085 1103 8 0.1763756417747402 1103 9 0.28806240882313006 1103 10-11 0.3336820958573554 1103 12-13 0.44219880699834135 1103 14-15 0.3947386931916341 1103 16-17 0.4766050823396881 1103 18-19 0.4858034684397339 1103 20-21 0.29540407281421466 1103 22-23 0.21867920789377138 1103 24-25 0.5981798145746922 1103 26-27 0.4368564063575775 1103 28-29 0.4848203452585551 1103 30-31 0.5214502014232352 1103 32-33 0.5962274035722501 1103 34-35 0.39649102248867507 1103 36-37 0.4138388411108025 1103 38-39 0.9038441623066689 1103 40-41 1.0643381299905812 1103 42-43 1.2145437159437265 1103 44-45 1.1693357886862188 1103 46-47 1.2117471767243089 1103 48-49 1.1198692621451478 1103 50-51 1.4263981117568534 1103 52-53 1.3476481712983492 1103 54-55 1.372282920154536 1103 56-57 1.2912827222879493 1103 58-59 1.600859433617078 1103 60-61 1.5727196511506634 1103 62-63 2.009288527722692 1103 64-65 1.5264498992080142 1103 66-67 1.530290463131447 1103 68-69 1.6765283504693258 1103 70-71 1.579156822220444 1103 72-73 1.6390698576278275 1103 74-75 1.511018371701656 1103 76-77 1.625960244341666 1103 78-79 1.663342530685572 1103 80-81 1.7931857342955873 1103 82-83 1.9970872043174488 1103 84-85 2.271184743468041 1103 86-87 2.3048710693219157 1103 88-89 2.283210385774499 1103 90-91 2.156496862465982 1103 92-93 2.1229826963414773 1103 94-95 2.121412580198349 1103 96-97 2.4510780006531157 1103 98-99 2.434672552445747 1103 100-101 1.8336736206349666 1104 1 0.22368702880303815 1104 2 0.1272288824710337 1104 3 0.07579206897240809 1104 4 0.05469788871967296 1104 5 0.06643214944213582 1104 6 0.06903364890258246 1104 7 0.24570187914660124 1104 8 0.10272005672283058 1104 9 -0.09451163194702872 1104 10-11 0.08776750256129873 1104 12-13 0.120383587495688 1104 14-15 0.5292231331668447 1104 16-17 0.27856642111891006 1104 18-19 0.39817352627697034 1104 20-21 0.4128564598387001 1104 22-23 0.5241475150217383 1104 24-25 0.20581295450061532 1104 26-27 0.3061484982364391 1104 28-29 0.23236289070445793 1104 30-31 0.2932409091463626 1104 32-33 -0.11199322622852748 1104 34-35 -0.00487251957578394 1104 36-37 0.1817248421758464 1104 38-39 -0.041573852728099325 1104 40-41 0.021631474546921936 1104 42-43 -0.004445579478051798 1104 44-45 0.205355551391051 1104 46-47 0.383805333729196 1104 48-49 0.7310245664017394 1104 50-51 0.7805596305093552 1104 52-53 0.702507383829353 1104 54-55 0.8860128201649751 1104 56-57 0.8077796123972156 1104 58-59 0.4663571548804839 1104 60-61 0.9185075231714244 1104 62-63 0.5771663361713948 1104 64-65 0.7131146983105268 1104 66-67 0.8504129823874003 1104 68-69 0.47934396771028887 1104 70-71 0.7310611430327292 1104 72-73 0.9532441410051433 1104 74-75 0.7068793826440825 1104 76-77 0.5635780957883902 1104 78-79 0.5832042076084285 1104 80-81 0.9407763783277296 1104 82-83 1.181490444893626 1104 84-85 1.398801921006001 1104 86-87 1.8868699091417298 1104 88-89 1.9028932786666104 1104 90-91 2.196624165152766 1104 92-93 2.274882392289701 1104 94-95 2.358119729841775 1104 96-97 2.4678305796755247 1104 98-99 2.4799975416433853 1104 100-101 2.388449849890799 1105 1 -0.10285755374316352 1105 2 -0.044530509106696314 1105 3 0.2518929521885127 1105 4 0.05495023617842065 1105 5 0.011760584539345587 1105 6 0.14853564607238923 1105 7 -0.01915699449145336 1105 8 0.21957505452698456 1105 9 0.24761436053831432 1105 10-11 0.2202324910941229 1105 12-13 0.2580830430664989 1105 14-15 0.3268028282586144 1105 16-17 0.1885648178063306 1105 18-19 0.15931410890681974 1105 20-21 0.2330749286054754 1105 22-23 0.2689935621591317 1105 24-25 0.11059222018345594 1105 26-27 -0.07608819252638455 1105 28-29 0.049077075838262374 1105 30-31 0.3741196638604407 1105 32-33 0.23778265855604275 1105 34-35 0.4200359870431143 1105 36-37 0.1343204658295818 1105 38-39 -0.19867339055582534 1105 40-41 0.4953936126688063 1105 42-43 0.2818739816325504 1105 44-45 0.5814362189267612 1105 46-47 0.4210398286508763 1105 48-49 0.6586384887317003 1105 50-51 0.6209728669003667 1105 52-53 0.9125859461459349 1105 54-55 0.5753598536881483 1105 56-57 0.6944867568975006 1105 58-59 0.7001668347059322 1105 60-61 0.7434034821266202 1105 62-63 1.1919844299024405 1105 64-65 1.0169710136193757 1105 66-67 0.4310700617095513 1105 68-69 0.8840110015123024 1105 70-71 1.080482207771702 1105 72-73 1.4231353248772116 1105 74-75 1.1091268086302932 1105 76-77 1.3004856543615837 1105 78-79 1.3854118993262716 1105 80-81 1.423882343347632 1105 82-83 1.44093639583226 1105 84-85 1.7992013549669537 1105 86-87 1.8909953021275854 1105 88-89 1.9981886018735153 1105 90-91 2.1653477192087998 1105 92-93 1.7492300880506804 1105 94-95 2.234762950966463 1105 96-97 2.1175974022585287 1105 98-99 1.9833373114634654 1105 100-101 2.1293419887794958 1106 1 -0.04749725802343363 1106 2 0.22122806158128938 1106 3 -0.0723288676598699 1106 4 0.3501320181602523 1106 5 0.12026177538225369 1106 6 -0.0056308014591479605 1106 7 -0.045585442488814465 1106 8 0.13333398584878609 1106 9 -0.05133604479906495 1106 10-11 -0.02511871364821161 1106 12-13 0.1372541863508232 1106 14-15 0.18662070818258059 1106 16-17 0.15365095669542939 1106 18-19 0.27506854733235286 1106 20-21 0.1068500884489012 1106 22-23 0.0896368427247225 1106 24-25 0.21352011596027864 1106 26-27 0.05152734177514873 1106 28-29 -0.1929668163021816 1106 30-31 -0.04092402542346463 1106 32-33 -0.0659927266835183 1106 34-35 0.40152194862057655 1106 36-37 0.19013531041894538 1106 38-39 0.49141218484293603 1106 40-41 0.3135943504654932 1106 42-43 0.2963053447532431 1106 44-45 0.2732505826710394 1106 46-47 0.5478386776279542 1106 48-49 0.25175714476968736 1106 50-51 0.6382652582782136 1106 52-53 0.5134529699402499 1106 54-55 0.6686610805944362 1106 56-57 0.3634482066454332 1106 58-59 0.5573926590922902 1106 60-61 0.4914301389760318 1106 62-63 0.5919160597418838 1106 64-65 0.46821933253987424 1106 66-67 0.8277559872236431 1106 68-69 0.7153651063515589 1106 70-71 0.7186462811306278 1106 72-73 0.5902823357488209 1106 74-75 0.856207553038594 1106 76-77 0.7575191407138462 1106 78-79 0.9173370077042442 1106 80-81 1.181491341161287 1106 82-83 1.1739892361992048 1106 84-85 1.3260122250711603 1106 86-87 1.512736261864326 1106 88-89 1.9268079002904805 1106 90-91 2.187831734866318 1106 92-93 1.905945765854419 1106 94-95 1.9562386945858705 1106 96-97 1.9818638505413837 1106 98-99 2.0189927258542575 1106 100-101 1.9976084671151693 1107 1 -0.0842459580369379 1107 2 0.12369367245144858 1107 3 0.11135041521521671 1107 4 0.2535954654050059 1107 5 0.2500430746771798 1107 6 0.06418151967950081 1107 7 0.04785386567796479 1107 8 0.3048564602195398 1107 9 0.4416593414426515 1107 10-11 0.40641917284872164 1107 12-13 0.09632231255901047 1107 14-15 0.26762904983122127 1107 16-17 0.5086254838757611 1107 18-19 0.2959251388154982 1107 20-21 0.28225687966398283 1107 22-23 0.40892472345858266 1107 24-25 0.5355973280779054 1107 26-27 0.37678165832309674 1107 28-29 0.49550076389521536 1107 30-31 0.44570562579484374 1107 32-33 0.6813192075053891 1107 34-35 0.45332217058155067 1107 36-37 0.6087251996689886 1107 38-39 0.6315299822261693 1107 40-41 0.7942345135442253 1107 42-43 0.592090556357121 1107 44-45 0.780167103614076 1107 46-47 0.8281845942378112 1107 48-49 0.5394830987243147 1107 50-51 0.784948851938168 1107 52-53 1.2353875869587938 1107 54-55 1.0712798247696078 1107 56-57 1.1887313386002383 1107 58-59 1.2003310731581607 1107 60-61 1.4621125207423056 1107 62-63 1.1431490298364295 1107 64-65 1.1366898436757964 1107 66-67 1.3298158771652524 1107 68-69 1.1895094534273696 1107 70-71 0.8856356803540955 1107 72-73 1.2041921089468666 1107 74-75 1.4928609152202625 1107 76-77 0.8899028828966387 1107 78-79 1.1761783978253355 1107 80-81 1.5090270146349773 1107 82-83 1.5248480999830178 1107 84-85 1.6718143966002188 1107 86-87 1.0678156771256226 1107 88-89 1.1084075340878279 1107 90-91 1.378779909945436 1107 92-93 1.361102406406097 1107 94-95 1.5857672415978001 1107 96-97 2.1339976725840195 1107 98-99 1.9331911470992793 1107 100-101 1.748060457354157 1108 1 0.05951966809774234 1108 2 0.17104150891260161 1108 3 0.18495015793512337 1108 4 0.23745321441167988 1108 5 0.15925847413564753 1108 6 0.18840215810760697 1108 7 0.1677640251293795 1108 8 0.012106986710051615 1108 9 0.021623654203949627 1108 10-11 0.2135265354165412 1108 12-13 0.0767902925990569 1108 14-15 0.14307384354030717 1108 16-17 0.2371119922321867 1108 18-19 0.2792252747174828 1108 20-21 -0.07417460394997022 1108 22-23 0.024731887879227088 1108 24-25 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-2.8428473849936253 2316 60-61 -2.374359998356578 2316 62-63 -2.2898583361782983 2316 64-65 -2.381971052620358 2316 66-67 -2.2783101852407164 2316 68-69 -2.98457044601448 2316 70-71 -2.466047561473925 2316 72-73 -2.151411858671324 2316 74-75 -2.472635720901213 2316 76-77 -2.12863426956228 2316 78-79 -1.9092895167002375 2316 80-81 -1.9079180756654601 2316 82-83 -2.0031000969390185 2316 84-85 -1.808798526164086 2316 86-87 -2.322933168474634 2316 88-89 -2.923756094341993 2316 90-91 -2.7356359214503314 2316 92-93 -2.774680403621062 2316 94-95 -2.8516949025572593 2316 96-97 -1.9811752757105943 2316 98-99 -2.167681246953199 2316 100-101 -2.0811437395157757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 44.0 5 105.0 6 262.0 7 560.0 8 1178.0 9 2048.0 10 3074.0 11 4662.0 12 6334.0 13 8534.0 14 10960.0 15 14577.0 16 17382.0 17 20193.0 18 16433.0 19 12437.0 20 12036.0 21 12568.0 22 13386.0 23 14726.0 24 16456.0 25 18981.0 26 21857.0 27 25532.0 28 28957.0 29 32778.0 30 36817.0 31 41347.0 32 46453.0 33 51598.0 34 57188.0 35 60808.0 36 55304.0 37 36534.0 38 9315.0 39 196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.488471782042545 24.254302439161506 16.229735142618214 16.02749063617773 2 17.82098630106185 28.094065667211925 35.32619999213028 18.758748039595947 3 25.074936446305035 28.163877892588985 30.377190652653237 16.383995008452747 4 14.921813961069985 20.85153083879961 37.166332226025666 27.06032297410474 5 11.364476315342015 43.026835914060996 32.53515565167414 13.073532118922854 6 26.817923770017256 42.19657240499357 15.682455637432621 15.30304818755656 7 25.423250088528405 37.62457211270931 20.510064133911158 16.44211366485113 8 26.785884918694393 33.78693601785182 18.400137148767065 21.027041914686723 9 26.120516668633964 19.353995582011027 21.074538106560095 33.45094964279491 10-11 24.79406606748394 29.574810717838893 25.821557650259404 19.809565564417763 12-13 28.896466151044358 27.022463857724215 26.3958759583605 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19.338572032820565 28.119998257514116 35.277329275027505 17.264100434637808 40-41 20.147913008714447 28.092510972978403 33.1982921007905 18.561283917516647 42-43 19.260739485359114 29.01421157583653 32.54230237927608 19.182746559528276 44-45 21.214588894267823 26.798791776831425 32.31933740243261 19.66728192646814 46-47 21.873478412694176 29.152084605348115 31.04983112796123 17.924605853996475 48-49 21.73602367515795 29.075139959979314 30.702455763653123 18.486380601209614 50-51 19.938381333127236 29.771652602809024 31.239399411215018 19.050566652848723 52-53 21.355174102472404 30.932549036690833 28.971292185395477 18.74098467544129 54-55 20.527822355126737 29.476744676246657 29.270806692316988 20.724626276309614 56-57 21.545657244086907 29.466695618138516 29.55811762027334 19.429529517501233 58-59 23.544244727683665 28.441342493941967 28.844602306269433 19.169810472104935 60-61 20.92522668960443 30.183326411245126 30.49352029296245 18.397926606187994 62-63 20.856425899652653 29.20073896212222 30.65252266743121 19.29031247079392 64-65 20.725041602014887 30.994414648406753 29.20812813420585 19.072415615372506 66-67 21.601342297912286 29.30374619439852 28.949200797340698 20.145710710348496 68-69 21.07967851203261 29.775437605435982 28.730510371633518 20.41437351089789 70-71 22.110054936832128 30.215749495352608 28.88255293615903 18.791642631656234 72-73 22.15916715790598 28.5143166691375 29.93716586651453 19.38935030644199 74-75 20.400055928293746 32.09827360036986 28.349532162633295 19.152138308703105 76-77 21.539018229468574 29.575231249714562 28.452725988849426 20.43302453196744 78-79 21.265140751546223 30.07997773945892 28.886003263192954 19.768878245801908 80-81 21.754797979265618 29.91489388614712 28.96924697497693 19.36106115961033 82-83 21.50435021534989 29.6175844130108 29.294893372114352 19.58317199952496 84-85 20.12529817000575 30.592066885664366 29.461164454902356 19.82147048942753 86-87 20.899786735436 30.297692034726637 27.826141949174872 20.976379280662492 88-89 20.43385021329118 30.555217779114823 27.933518098998878 21.07741390859512 90-91 21.645908355082756 28.6672060383385 28.57174990493047 21.11513570164827 92-93 21.868426656508916 30.545784732027208 28.04038549793483 19.545403113529044 94-95 21.208487921235193 30.174949345301638 28.407674915962044 20.20888781750112 96-97 19.810316733938713 30.17942023728685 29.175892848356018 20.83437018041842 98-99 21.011101761815084 30.67040076144041 27.453188496569936 20.86530898017457 100-101 20.716934573920256 30.027593334012913 27.356628903050733 21.898843189016095 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1508.0 1 1063.5 2 782.5 3 926.5 4 757.0 5 631.5 6 996.0 7 1855.5 8 2578.5 9 2864.0 10 2512.0 11 1988.0 12 1897.5 13 1894.5 14 2023.0 15 2190.0 16 2300.0 17 2468.5 18 2629.0 19 2781.5 20 3019.5 21 3333.5 22 3633.0 23 4086.5 24 4623.0 25 5339.5 26 6526.0 27 7603.5 28 8453.0 29 9708.5 30 11464.5 31 13336.0 32 15617.0 33 18022.5 34 20202.5 35 21914.0 36 23684.0 37 25390.5 38 25026.5 39 23740.0 40 22821.0 41 22160.5 42 21749.0 43 21757.0 44 21838.5 45 21958.5 46 22648.5 47 29335.5 48 42029.5 49 41278.5 50 28386.5 51 19718.5 52 15331.0 53 13196.0 54 11758.5 55 10353.0 56 9079.0 57 7982.5 58 7094.0 59 6239.5 60 5413.0 61 4572.5 62 3633.5 63 2682.5 64 1917.0 65 1409.5 66 1045.0 67 785.0 68 603.5 69 479.5 70 383.0 71 290.5 72 242.5 73 198.0 74 157.5 75 131.0 76 103.0 77 79.0 78 52.0 79 41.0 80 34.5 81 21.5 82 14.0 83 8.5 84 4.5 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017003074605556775 2 0.00674502133112996 3 0.004637202165151847 4 0.0 5 0.0021078191659781127 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.620851109274967E-4 14-15 2.8104255546374836E-4 16-17 0.0 18-19 9.836489441231193E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.00168625533278249 28-29 0.0034427713044309174 30-31 0.0011944308607209303 32-33 0.007798930914119016 34-35 0.0018267766105143641 36-37 7.728670275253079E-4 38-39 0.0012646914995868674 40-41 2.1078191659781124E-4 42-43 0.004637202165151847 44-45 0.0017565159716484268 46-47 3.513031943296854E-4 48-49 5.620851109274967E-4 50-51 1.4052127773187418E-4 52-53 7.026063886593709E-5 54-55 0.0019672978882462385 56-57 0.014122388412053355 58-59 0.025855915102664842 60-61 0.02044584590998769 62-63 0.01370082457885773 64-65 0.018548808660607388 66-67 0.0028806861935034202 68-69 0.0010539095829890563 70-71 7.026063886593709E-5 72-73 7.026063886593709E-5 74-75 0.0014754734161846787 76-77 0.001897037249380301 78-79 0.008852840497108072 80-81 0.015808643744835843 82-83 0.01679229268895896 84-85 0.029931032156889196 86-87 0.011030920301952121 88-89 0.0399783035147182 90-91 0.044193941846674425 92-93 0.027050345963385777 94-95 0.02831503746297264 96-97 0.04847984081749659 98-99 0.04953375040048564 100-101 0.05810554834212996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 711636.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.23947113960712 #Duplication Level Percentage of deduplicated Percentage of total 1 81.7427424086512 44.33683117746375 2 10.900601766788967 11.824857498682013 3 3.157514311039183 5.137857190395186 4 1.3180908223120298 2.8597019646469746 5 0.7117293822407991 1.9301912643630115 6 0.41913577358110266 1.3640221616837473 7 0.27038419082764475 1.0265846860501444 8 0.19761712648263585 0.8574918742837614 9 0.15996023835788376 0.78085428587074 >10 0.9748964336678422 10.0376634555535 >50 0.09198231787260441 3.4268153164296313 >100 0.04703045211384113 4.8797015899507 >500 0.003377877776119786 1.3835498645000512 >1k 0.0041573880321474285 5.94375389481768 >5k 5.196735040184286E-4 1.9878752000380182 >10k+ 2.598367520092143E-4 2.2222485752711427 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15768 2.215739507276192 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7286 1.0238380295544351 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6819 0.95821459285365 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4866 0.6837765374432997 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4709 0.6617146968393954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4659 0.6546886329528017 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3988 0.5603988555947141 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3228 0.4536026845184898 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3025 0.42507686513891935 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2663 0.3742081625999809 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2474 0.34764964110865665 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2115 0.29720250240291385 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2034 0.28582027890663203 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1812 0.254624555250156 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.2207589273167743 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1394 0.19588666115823256 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1386 0.1947624909363776 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1225 0.17213856522154586 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1025 0.144034309675171 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 994 0.13967815006548293 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 990 0.13911606495455542 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 939 0.13194947979022983 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 915 0.12857696912466487 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 861 0.12098882012714365 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 742 0.10426678807705062 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4052127773187415E-4 0.0 12-13 0.0 0.0 0.0 1.4052127773187415E-4 0.0 14-15 0.0 0.0 0.0 1.4052127773187415E-4 0.0 16-17 0.0 0.0 0.0 1.4052127773187415E-4 0.0 18-19 0.0 0.0 0.0 1.4052127773187415E-4 0.0 20-21 0.0 0.0 0.0 3.513031943296854E-4 0.0 22-23 0.0 0.0 0.0 8.43127666391245E-4 7.026063886593708E-5 24-25 0.0 0.0 0.0 0.0013349521384528045 1.4052127773187415E-4 26-27 0.0 0.0 0.0 0.001897037249380301 1.4052127773187415E-4 28-29 0.0 0.0 0.0 0.004145377693090288 1.4052127773187415E-4 30-31 0.0 0.0 0.0 0.007377367080923393 1.4052127773187415E-4 32-33 0.0 0.0 0.0 0.011522744774013681 1.4052127773187415E-4 34-35 0.0 0.0 0.0 0.016792292688958963 1.4052127773187415E-4 36-37 0.0 0.0 0.0 0.025434351269469222 1.4052127773187415E-4 38-39 0.0 0.0 0.0 0.037097617321214776 1.4052127773187415E-4 40-41 0.0 0.0 0.0 0.05557616534295623 1.4052127773187415E-4 42-43 0.0 0.0 0.0 0.07286028250397675 1.4052127773187415E-4 44-45 1.4052127773187415E-4 0.0 0.0 0.0956949901354063 1.4052127773187415E-4 46-47 1.4052127773187415E-4 0.0 0.0 0.13223052234569357 1.4052127773187415E-4 48-49 1.4052127773187415E-4 0.0 0.0 0.16117790555845968 1.4052127773187415E-4 50-51 1.4052127773187415E-4 0.0 0.0 0.19223310793720386 1.4052127773187415E-4 52-53 1.4052127773187415E-4 0.0 0.0 0.22061840603904243 1.4052127773187415E-4 54-55 1.4052127773187415E-4 0.0 0.0 0.25153308714005473 1.4052127773187415E-4 56-57 1.4052127773187415E-4 0.0 0.0 0.3105520237874419 1.4052127773187415E-4 58-59 1.4052127773187415E-4 0.0 0.0 0.3653553221028728 1.4052127773187415E-4 60-61 1.4052127773187415E-4 0.0 0.0 0.4175589767802641 1.4052127773187415E-4 62-63 1.4052127773187415E-4 0.0 0.0 0.47060575912404656 1.4052127773187415E-4 64-65 1.4052127773187415E-4 0.0 0.0 0.5267440095779303 1.4052127773187415E-4 66-67 1.4052127773187415E-4 0.0 0.0 0.5968641271661355 1.4052127773187415E-4 68-69 1.4052127773187415E-4 0.0 0.0 0.6794906384724775 1.4052127773187415E-4 70-71 1.4052127773187415E-4 0.0 0.0 0.7758882349965432 1.4052127773187415E-4 72-73 1.4052127773187415E-4 0.0 0.0 0.8680701931886526 1.4052127773187415E-4 74-75 1.4052127773187415E-4 0.0 0.0 0.9730395876543626 1.4052127773187415E-4 76-77 1.4052127773187415E-4 0.0 0.0 1.0893912056163544 1.4052127773187415E-4 78-79 1.4052127773187415E-4 0.0 0.0 1.233987600402453 1.4052127773187415E-4 80-81 1.4052127773187415E-4 0.0 0.0 1.3907390857123585 1.4052127773187415E-4 82-83 1.4052127773187415E-4 0.0 0.0 1.5425723263016486 1.4052127773187415E-4 84-85 1.4052127773187415E-4 0.0 0.0 1.7145001096065966 1.4052127773187415E-4 86-87 1.4052127773187415E-4 0.0 0.0 1.8777858343310343 1.4052127773187415E-4 88-89 1.4052127773187415E-4 0.0 0.0 2.068192165657724 1.4052127773187415E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 7585 0.0 20.945286 12-13 AGTACTT 7650 0.0 20.456896 12-13 ATACTGC 165 6.323747E-5 20.149294 6 GTACTTT 7905 0.0 19.857079 14-15 GCGCTCA 120 0.004764786 19.789484 9 TAGCCCT 365 5.456968E-12 19.518396 4 GGTACAC 150 7.7155314E-4 19.000576 3 CTACACG 150 7.7228283E-4 18.997906 4 GTAAGGT 205 1.5895157E-5 18.534544 4 GTACACA 335 7.3123374E-10 18.430805 4 CCTAGAC 155 9.6176536E-4 18.387653 3 TACTTTT 8530 0.0 18.179415 14-15 GGTACTC 185 1.5199538E-4 17.973518 3 ACTTTTT 8870 0.0 17.910936 16-17 GTAGTAA 240 3.0796527E-6 17.816797 1 GGGATCG 165 0.0014641513 17.270823 7 AGTCTAC 170 0.0017837804 16.76875 1 GTGGTAC 255 5.4876364E-6 16.76875 1 TCGGGAG 170 0.0017862982 16.765213 3 TGTATGG 285 8.562092E-7 16.668343 2 >>END_MODULE