FastQCFastQC Report
Thu 26 May 2016
SRR936675_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936675_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309301
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81470.35279073624443064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65550.28385212668248966No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53070.2298097995887067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37640.162993044215544No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35730.15472214319397948No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26360.11414709472693252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122450.027.4650081
GTATCAA169000.026.505151
TACATGG127850.026.0465852
ACATGGG123900.025.0749443
GAGTACT82550.023.27969612-13
TCAACGC195700.022.5040174
ATCAACG196700.022.3876693
CAACGCA197450.022.2112065
GGTATCA122250.022.1478371
TATCAAC201250.021.9523282
GTACTTT87200.021.82083114-15
AACGCAG201800.021.7098186
TACCTGG17400.021.2950612
GTACCTG19450.020.7589131
AGTACTT88050.020.6654712-13
CATGGGA68500.020.3905014
CGCGGAA3500.020.35967482-83
AGAGTAC157500.020.10699810-11
TCGCGCT3900.020.0899560-61
CATGGGG50150.020.0833454
Produced by FastQC (version 0.11.5)