FastQCFastQC Report
Thu 26 May 2016
SRR936675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309301
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101190.4381845415560813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88040.38124090363274427No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67880.2939417598658642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51200.22171211115398123No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29440.1274844639135392No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.11761134646371349No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24600.10652574090601442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG124850.024.9250681
TATAACG1000.001653196923.752352
GTATCAA210450.023.3203471
TACATGG130950.023.289822
ACATGGG128100.022.767653
GAGTACT109600.022.73155812-13
TACCTGG18000.022.4327752
GGTATCA159100.021.70941
GTACTTT115600.021.45015514-15
GTACCTG19800.021.1154841
TAAGGTG11100.020.5399045
AGTACTT116200.020.27541712-13
ATCAACG243550.020.0300143
TCAACGC243650.020.0209274
CAACGCA244700.019.9544285
ATGGGTA10250.019.9262125
CATGGGA72300.019.8403264
TATCAAC247000.019.7519552
AACGCAG248650.019.6930396
AGAGTAC192200.019.12833410-11
Produced by FastQC (version 0.11.5)