##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936675_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2309301 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00112025240538 31.0 31.0 34.0 30.0 34.0 2 32.12908884549913 33.0 31.0 34.0 30.0 34.0 3 32.24664606302946 34.0 31.0 34.0 30.0 34.0 4 35.96397697831508 37.0 35.0 37.0 35.0 37.0 5 35.84052317129729 37.0 35.0 37.0 35.0 37.0 6 35.8952600808643 37.0 35.0 37.0 35.0 37.0 7 35.82500245745358 37.0 35.0 37.0 35.0 37.0 8 35.79424812962883 37.0 35.0 37.0 33.0 37.0 9 37.42315445236459 39.0 37.0 39.0 34.0 39.0 10-11 37.328201260901025 39.0 37.0 39.0 34.0 39.0 12-13 37.20965997936172 39.0 37.0 39.0 33.0 39.0 14-15 38.32706303768976 40.0 38.0 41.0 33.0 41.0 16-17 38.310501099683414 40.0 38.0 41.0 33.5 41.0 18-19 38.454497269953116 40.0 38.0 41.0 34.0 41.0 20-21 38.479794535229495 40.0 38.0 41.0 34.0 41.0 22-23 38.38634158128369 40.0 38.0 41.0 34.0 41.0 24-25 38.27712000297926 40.0 38.0 41.0 34.0 41.0 26-27 38.09015412022946 40.0 37.0 41.0 33.0 41.0 28-29 38.101475511420986 40.0 37.5 41.0 33.0 41.0 30-31 37.972542124218535 40.0 37.0 41.0 33.0 41.0 32-33 37.78043377628122 40.0 37.0 41.0 33.0 41.0 34-35 37.60140514380758 40.0 36.5 41.0 33.0 41.0 36-37 37.418826302850945 40.0 36.0 41.0 32.5 41.0 38-39 37.228812311604244 39.0 36.0 41.0 32.0 41.0 40-41 37.0035489093886 39.0 36.0 41.0 31.0 41.0 42-43 36.728709250115074 39.0 35.0 41.0 31.0 41.0 44-45 36.418375300577964 39.0 35.0 40.0 30.0 41.0 46-47 35.99880786437108 39.0 35.0 40.0 29.5 41.0 48-49 35.574842127552884 39.0 35.0 40.0 28.0 41.0 50-51 35.257566034050996 38.0 35.0 40.0 27.0 41.0 52-53 34.99968042277728 38.0 34.0 40.0 26.5 41.0 54-55 34.66563302055471 38.0 34.0 40.0 26.0 41.0 56-57 34.33584340023236 38.0 33.5 40.0 25.0 41.0 58-59 33.97702443293447 37.0 33.0 40.0 24.0 41.0 60-61 33.60942488657823 37.0 33.0 39.5 23.0 41.0 62-63 33.29978681860875 36.0 32.0 39.0 23.0 40.0 64-65 32.99652492247654 36.0 32.0 39.0 22.0 40.0 66-67 32.55424390324172 35.5 31.5 39.0 21.0 40.0 68-69 32.10532104736455 35.0 31.0 38.0 19.5 40.0 70-71 31.621286484524973 35.0 31.0 37.5 18.5 39.5 72-73 31.0728183549914 34.5 30.0 37.0 16.5 39.0 74-75 30.548072771804108 34.0 29.5 36.0 12.5 39.0 76-77 29.163534333549414 32.5 28.0 35.0 11.0 37.0 78-79 29.932129895583124 34.0 29.0 35.0 9.0 37.0 80-81 29.776363497006237 34.0 29.0 35.0 7.0 37.0 82-83 29.39047421708993 34.0 29.0 35.0 6.0 36.5 84-85 28.959504412807163 34.0 29.0 35.0 2.0 36.0 86-87 28.55984971209903 33.0 29.0 35.0 2.0 36.0 88-89 28.079805101197287 33.0 27.5 35.0 2.0 35.0 90-91 27.52920277607813 33.0 26.5 34.5 2.0 35.0 92-93 26.976660036954904 32.0 25.5 34.0 2.0 35.0 94-95 26.266420878005942 32.0 25.0 34.0 2.0 35.0 96-97 25.47995064307338 32.0 23.5 34.0 2.0 35.0 98-99 24.40809037020293 31.0 10.5 34.0 2.0 35.0 100-101 20.66589998445417 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29309323747475347 1101 2 0.3542371360825243 1101 3 0.26469205415108377 1101 4 0.21568089608587115 1101 5 0.1824324760164231 1101 6 0.18321378124840493 1101 7 0.2175165832919177 1101 8 0.2017509122634351 1101 9 0.289724390318419 1101 10-11 0.28038961078098623 1101 12-13 0.2987298979325743 1101 14-15 0.35305114139536187 1101 16-17 0.45251029764936845 1101 18-19 0.4246453468070328 1101 20-21 0.36752951730164085 1101 22-23 0.38804831724841193 1101 24-25 0.2884896574663216 1101 26-27 0.2733303960900031 1101 28-29 0.2733065894516713 1101 30-31 0.4143499078276278 1101 32-33 0.3690941988902452 1101 34-35 0.3581051802422692 1101 36-37 0.3764184293501174 1101 38-39 0.4211466452927368 1101 40-41 0.49753252289124106 1101 42-43 0.5876636751839612 1101 44-45 0.7005147309812685 1101 46-47 0.5647850534068724 1101 48-49 0.6281524367356042 1101 50-51 0.749608269267263 1101 52-53 0.5734808385140866 1101 54-55 0.5080932196193331 1101 56-57 0.48409432563102683 1101 58-59 0.49095036492605715 1101 60-61 0.5345954812996752 1101 62-63 0.41439650579925313 1101 64-65 0.5399266217146206 1101 66-67 0.511735807512359 1101 68-69 0.5232560836299314 1101 70-71 0.4532077064168014 1101 72-73 0.45139055468057165 1101 74-75 0.4172680750315685 1101 76-77 0.5355520489717129 1101 78-79 0.6172392408521645 1101 80-81 0.6018358902339926 1101 82-83 0.4818661216767417 1101 84-85 0.38784794797523503 1101 86-87 0.40694553498402897 1101 88-89 0.4886891753611593 1101 90-91 0.5554527914020753 1101 92-93 0.6213935533904404 1101 94-95 0.6247894441035875 1101 96-97 0.6569941510340449 1101 98-99 0.3170568837867229 1101 100-101 0.4588351303606437 1102 1 0.23448953330001743 1102 2 0.3655452257759322 1102 3 0.2540333961607786 1102 4 0.19791308057864399 1102 5 0.037880342489025054 1102 6 0.14688961767875242 1102 7 0.18464064888745924 1102 8 0.14758062455871368 1102 9 0.29171493087168443 1102 10-11 0.35382249616542083 1102 12-13 0.2822500532780481 1102 14-15 0.4253084405837839 1102 16-17 0.44491178480674876 1102 18-19 0.3619119664885275 1102 20-21 0.38081364867644396 1102 22-23 0.377596028987881 1102 24-25 0.3660420624243699 1102 26-27 0.346610270537127 1102 28-29 0.38178413136150624 1102 30-31 0.5719887083985213 1102 32-33 0.5446833388377712 1102 34-35 0.5633865378430372 1102 36-37 0.487828202502385 1102 38-39 0.5647875578414272 1102 40-41 0.5309415300320524 1102 42-43 0.5972664178700526 1102 44-45 0.654318160428133 1102 46-47 0.4260866370075078 1102 48-49 0.7006607798427922 1102 50-51 0.6589916447577266 1102 52-53 0.7846084062127758 1102 54-55 0.7853469036575618 1102 56-57 0.8065756581494838 1102 58-59 0.75547801375955 1102 60-61 0.7092081185458525 1102 62-63 0.611806668728299 1102 64-65 0.5650264504954592 1102 66-67 0.718941319968728 1102 68-69 0.7046424171846581 1102 70-71 0.4293644955981968 1102 72-73 0.3958670144004941 1102 74-75 0.41946366054459006 1102 76-77 0.5072245652340008 1102 78-79 0.5652178037180953 1102 80-81 0.7952121284523699 1102 82-83 0.5894333257878834 1102 84-85 0.5110417712497366 1102 86-87 0.7686876782146292 1102 88-89 0.6845087310862858 1102 90-91 0.44803079680477964 1102 92-93 0.7222998138414063 1102 94-95 0.6693475685354109 1102 96-97 0.7896191592770592 1102 98-99 0.9324520067489281 1102 100-101 0.6062780592681882 1103 1 0.24853330525219874 1103 2 0.32787038902767307 1103 3 0.2280032052049492 1103 4 0.1649248616839074 1103 5 0.0839539943818437 1103 6 0.19436611667201475 1103 7 0.24141918734093082 1103 8 0.19482354990007877 1103 9 0.26838613786041066 1103 10-11 0.3272044677768591 1103 12-13 0.1535999965708612 1103 14-15 0.3371792749788156 1103 16-17 0.31823125673044217 1103 18-19 0.26348292104283644 1103 20-21 0.2773994607633128 1103 22-23 0.29894088237357863 1103 24-25 0.2667739629644643 1103 26-27 0.351606888715402 1103 28-29 0.3431661785408764 1103 30-31 0.38918859748226 1103 32-33 0.4542203883653855 1103 34-35 0.4551117228283701 1103 36-37 0.4059139540099963 1103 38-39 0.2791977874930467 1103 40-41 0.43112665588702725 1103 42-43 0.5548770936848086 1103 44-45 0.4384160883675463 1103 46-47 0.36131522692212314 1103 48-49 0.4851870562541052 1103 50-51 0.47264733471180875 1103 52-53 0.45106632423466664 1103 54-55 0.5087035037994596 1103 56-57 0.599624016014296 1103 58-59 0.3131059890132377 1103 60-61 0.20561926562979949 1103 62-63 0.40748280824296756 1103 64-65 0.3304527723724391 1103 66-67 0.2558294069630449 1103 68-69 0.3339847637130262 1103 70-71 0.26230718636666595 1103 72-73 0.492640747107302 1103 74-75 0.5207253329818968 1103 76-77 0.464190103127887 1103 78-79 0.3484532578252413 1103 80-81 0.5930677162335982 1103 82-83 0.41155574835884323 1103 84-85 0.6149270097696551 1103 86-87 0.4957179890799246 1103 88-89 0.4890609816371203 1103 90-91 0.4061181439826633 1103 92-93 0.5317586724031891 1103 94-95 0.5749240926347134 1103 96-97 0.6640289017964562 1103 98-99 0.6707545882998609 1103 100-101 0.59817995458722 1104 1 0.2150527569791869 1104 2 0.27247768877723644 1104 3 0.2262225495999317 1104 4 0.15155352226256014 1104 5 0.10345909411849874 1104 6 0.15096762166618305 1104 7 0.09436143333650904 1104 8 0.10944944213405705 1104 9 0.22562576976441306 1104 10-11 0.2713460756455177 1104 12-13 0.20968947510152702 1104 14-15 0.25368553014746453 1104 16-17 0.2787365969377191 1104 18-19 0.15708464304707093 1104 20-21 0.25821401099528174 1104 22-23 0.1647025583951347 1104 24-25 0.09294831002808479 1104 26-27 0.1640626802449674 1104 28-29 0.16596455272489408 1104 30-31 0.2585714091116529 1104 32-33 0.20028106907318488 1104 34-35 0.09784804386250556 1104 36-37 0.09974207030393245 1104 38-39 0.19386532219998998 1104 40-41 0.20400508919161808 1104 42-43 0.15397914120414669 1104 44-45 0.2036809872819063 1104 46-47 0.2072738542454573 1104 48-49 0.18890027451345048 1104 50-51 0.17129131502840522 1104 52-53 0.3311946534330801 1104 54-55 0.14803980489212876 1104 56-57 0.17998284509833695 1104 58-59 0.34351789758748197 1104 60-61 0.1718806513270792 1104 62-63 0.22967531377086203 1104 64-65 0.2554417752404916 1104 66-67 0.348878633058213 1104 68-69 0.2716618175373924 1104 70-71 0.1683482775167633 1104 72-73 0.2559824675866018 1104 74-75 0.3626716200568971 1104 76-77 0.24590377886792325 1104 78-79 0.3742328811661437 1104 80-81 0.2869808770145994 1104 82-83 0.12564028844412078 1104 84-85 0.11088768989094433 1104 86-87 0.11367703869312251 1104 88-89 0.2189203943022946 1104 90-91 0.2585771026862318 1104 92-93 0.35141567918915584 1104 94-95 0.38795642713520806 1104 96-97 0.5806735741992277 1104 98-99 0.4089031991421521 1104 100-101 0.1776678108209815 1105 1 0.1666741385892827 1105 2 0.2541312907242741 1105 3 0.17740272500755339 1105 4 0.11888890807486518 1105 5 0.19155753121462737 1105 6 0.14463155254728832 1105 7 0.15664111814534465 1105 8 0.09761467436872096 1105 9 0.17306183453266044 1105 10-11 0.17449158923871977 1105 12-13 0.16319494027464287 1105 14-15 0.3372083722934107 1105 16-17 0.30439360774010993 1105 18-19 0.27179799977150054 1105 20-21 0.22706802650915137 1105 22-23 0.16639802886579957 1105 24-25 0.17997725714732127 1105 26-27 0.2688000166800606 1105 28-29 0.15466593750839763 1105 30-31 0.29399919230640137 1105 32-33 0.40539767905355717 1105 34-35 0.15644171188811384 1105 36-37 0.2755514815086286 1105 38-39 0.035706206455017764 1105 40-41 0.2129418180724585 1105 42-43 0.2984066096172171 1105 44-45 0.3248650270825948 1105 46-47 0.4714568166226414 1105 48-49 0.4210026323050684 1105 50-51 0.5756544963196291 1105 52-53 0.45879512125497257 1105 54-55 0.5280974892761421 1105 56-57 0.7068767176040751 1105 58-59 0.5954031327908282 1105 60-61 0.4229579468661413 1105 62-63 0.47683779998316567 1105 64-65 0.44360931075579657 1105 66-67 0.5331810077405521 1105 68-69 0.5564637481984036 1105 70-71 0.40432500926390347 1105 72-73 0.5859414947832278 1105 74-75 0.6908148943494687 1105 76-77 0.5706184919687267 1105 78-79 0.6454935690281118 1105 80-81 0.6723338035885718 1105 82-83 0.6380892643799605 1105 84-85 0.48920435003164897 1105 86-87 0.6113246214000903 1105 88-89 0.5658522965305899 1105 90-91 0.7384382404556824 1105 92-93 0.6928243144441915 1105 94-95 0.8669425741817633 1105 96-97 0.9245405776567068 1105 98-99 0.9004890590463859 1105 100-101 0.6182398433637779 1106 1 0.03618930255401054 1106 2 0.20606842700589567 1106 3 0.10186046328177412 1106 4 0.06295544370430406 1106 5 0.12536716796624603 1106 6 0.002216249912592616 1106 7 0.07314629346697643 1106 8 0.06957673743495718 1106 9 0.22863534780056227 1106 10-11 0.1301018318624898 1106 12-13 0.1919965780180064 1106 14-15 0.18991913023807427 1106 16-17 0.2789942592379475 1106 18-19 0.1986021650273031 1106 20-21 0.111480436216155 1106 22-23 0.10422356220558271 1106 24-25 0.14898372550961625 1106 26-27 0.10499391967528027 1106 28-29 0.15684186582963378 1106 30-31 0.06748954491675363 1106 32-33 0.07282437530177788 1106 34-35 0.13882698900008705 1106 36-37 0.1517734668370352 1106 38-39 0.1921283539663321 1106 40-41 0.09682941292461322 1106 42-43 0.23427162180654193 1106 44-45 0.07551316958635113 1106 46-47 0.13283870783784124 1106 48-49 0.21605786379648606 1106 50-51 0.17350920140817294 1106 52-53 0.16610373373396925 1106 54-55 0.18890180240560284 1106 56-57 0.17357509343572275 1106 58-59 0.20121057158676336 1106 60-61 0.2224494732449287 1106 62-63 0.1459715318923358 1106 64-65 0.08054312841802158 1106 66-67 -0.05071398079412148 1106 68-69 0.039467157659345276 1106 70-71 0.20421541883805716 1106 72-73 0.3088688773803412 1106 74-75 0.2678792642958747 1106 76-77 0.0025288231630149482 1106 78-79 -0.06980978283723971 1106 80-81 0.025497830130014165 1106 82-83 0.12790485091156967 1106 84-85 0.20767767924296265 1106 86-87 0.11234462515328048 1106 88-89 -0.0589497346318133 1106 90-91 0.26499214748755406 1106 92-93 0.024050106750696187 1106 94-95 0.2600037141305158 1106 96-97 0.48290004074216597 1106 98-99 0.36023771482197375 1106 100-101 0.014436126680809735 1107 1 0.06805482737255986 1107 2 0.21947561838386065 1107 3 0.13595339846121846 1107 4 0.0550731909996216 1107 5 -0.029460160539883873 1107 6 0.05448729040323741 1107 7 0.04632830704250779 1107 8 0.019594369670286937 1107 9 0.05254088363604836 1107 10-11 0.08335435721902229 1107 12-13 0.14240169042243167 1107 14-15 0.19343708294249495 1107 16-17 0.2218007791323373 1107 18-19 0.10905151675307678 1107 20-21 0.1304707397737488 1107 22-23 0.15165908013426588 1107 24-25 0.12400421064919698 1107 26-27 0.1522614380089493 1107 28-29 0.24091279289052636 1107 30-31 0.2622981171861838 1107 32-33 0.12698914360734648 1107 34-35 0.11151264013579976 1107 36-37 0.21319962723975294 1107 38-39 0.23465207168239033 1107 40-41 0.19365312231791876 1107 42-43 0.2146416670840665 1107 44-45 0.06351535581192991 1107 46-47 0.08719103851046128 1107 48-49 0.11353795567287506 1107 50-51 0.10938655312364887 1107 52-53 0.11483854577262775 1107 54-55 0.26253255851532487 1107 56-57 0.10565572294512293 1107 58-59 0.2174309410657429 1107 60-61 0.24144586871837959 1107 62-63 0.0031742785741712964 1107 64-65 0.014862065095563537 1107 66-67 0.1163734570747792 1107 68-69 0.19340094797216523 1107 70-71 -0.06622729184141818 1107 72-73 0.2261688029903297 1107 74-75 0.22830309003618865 1107 76-77 0.08358493828821523 1107 78-79 0.15270079006883464 1107 80-81 0.1633783925425476 1107 82-83 0.2136320068706219 1107 84-85 0.21854814537748624 1107 86-87 0.31388407803059337 1107 88-89 0.20049389326709743 1107 90-91 0.27306985630942293 1107 92-93 0.2822645404727986 1107 94-95 0.3757411062242362 1107 96-97 0.6725990400377384 1107 98-99 0.7316775262643063 1107 100-101 0.8056181213799896 1108 1 0.05727078585499967 1108 2 0.1907818172238933 1108 3 0.014526712324624214 1108 4 0.13164192531742458 1108 5 0.1440501160153076 1108 6 0.018504264886672672 1108 7 0.12047735479866617 1108 8 0.20220872119840294 1108 9 0.18190430622938436 1108 10-11 0.132650372054556 1108 12-13 0.08907543550353836 1108 14-15 0.13238344467175267 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-1.5708665292409663 2316 58-59 -1.7551558535228011 2316 60-61 -1.4407194613313976 2316 62-63 -1.607731467756146 2316 64-65 -1.290905108106685 2316 66-67 -1.366329191895744 2316 68-69 -1.5413406162704142 2316 70-71 -1.3263179744299087 2316 72-73 -1.2569433285314915 2316 74-75 -1.3675375249465311 2316 76-77 -1.566455838948361 2316 78-79 -1.5661453477443317 2316 80-81 -1.5194742183228982 2316 82-83 -1.742202237670945 2316 84-85 -1.3566061721462503 2316 86-87 -1.6712544095881974 2316 88-89 -1.7584407038933527 2316 90-91 -2.2455007278578982 2316 92-93 -1.906466191882096 2316 94-95 -1.9632182031173322 2316 96-97 -2.059585155496329 2316 98-99 -1.6611667367579557 2316 100-101 -1.4570017118555434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 25.0 5 60.0 6 157.0 7 327.0 8 786.0 9 1572.0 10 2552.0 11 4195.0 12 5980.0 13 8760.0 14 11612.0 15 15797.0 16 20749.0 17 27557.0 18 24179.0 19 15159.0 20 13722.0 21 13981.0 22 14959.0 23 16578.0 24 19487.0 25 23513.0 26 28680.0 27 35446.0 28 43751.0 29 54810.0 30 67946.0 31 85119.0 32 107316.0 33 138599.0 34 184616.0 35 253938.0 36 359550.0 37 457317.0 38 240917.0 39 9573.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.773159175754216 19.383028297719378 14.718625217624792 22.125187308901612 2 15.756669991178423 20.89245601445751 39.37363773330949 23.97723626105458 3 19.77346937007942 25.058852060038628 29.78516183233875 25.3825167375432 4 13.036706007677667 17.037580733513906 36.89108580757331 33.03462745123512 5 13.501555128459414 38.00710569416933 33.66625756668951 14.825081610681753 6 31.8737574703341 36.5753966243465 16.880649166132955 14.670196739186448 7 29.008532048553388 33.2914879142664 20.045485782878135 17.654494254302076 8 27.110483107560956 33.988623185456106 19.25826390987928 19.64262979710366 9 27.001094271465064 16.845501600269174 20.321361590681846 35.832042537583916 10-11 25.371400263542952 26.75532552924023 26.609762001575373 21.263512205641447 12-13 26.702322477667483 24.193035035276907 27.359317819547996 21.745324667507614 14-15 23.223222074533442 25.473506848763392 26.357351657038507 24.94591941966465 16-17 22.102113754103677 29.022535422302614 26.877796682760202 21.997554140833504 18-19 21.867957010387116 26.971398357609193 29.88246644860216 21.278178183401533 20-21 22.736235539112787 25.84786359614072 30.151373398614595 21.2645274661319 22-23 22.355584462936893 25.76035241985527 30.01895386939108 21.865109247816754 24-25 22.504718434447593 26.115173320325024 30.06467129448899 21.3154369507384 26-27 22.0457399039058 26.315070412136336 30.171266444131362 21.4679232398265 28-29 21.628237641658153 26.57378284030164 30.230669416994456 21.56731010104575 30-31 21.852213595936927 26.1332130652533 30.116284885519782 21.89828845328999 32-33 21.725086598167614 26.42728373842525 30.093767227070163 21.753862436336977 34-35 22.592885372070874 26.212361447885694 29.640501202861753 21.554251977181675 36-37 22.43808385541334 25.9530869570395 29.762122053590474 21.846707133956688 38-39 21.98637826672051 26.499175572254625 29.84465247907534 21.669793681949525 40-41 22.194695837417363 26.306159075511857 29.860050634402334 21.639094452668438 42-43 22.048976072746783 26.504538987528747 29.269806200702774 22.176678739021696 44-45 22.218748118895746 26.491274491165363 29.12464721852076 22.16533017141813 46-47 22.702822195980517 26.911519979422344 28.449301325379412 21.93635649921773 48-49 22.899416803886098 27.05330609514097 28.069736701082494 21.97754039989044 50-51 22.47543864017486 27.17091025617431 28.18664360018924 22.167007503461594 52-53 22.944522931856685 27.37322659045268 27.702765463373442 21.979485014317188 54-55 22.34919467793938 27.17638533170426 27.94311333747746 22.5313066528789 56-57 22.61219914967408 27.477125349231724 27.68461803978091 22.22605746131329 58-59 23.03924519248882 26.95616457457418 27.65896821090475 22.345622022032252 60-61 22.629458504359366 27.233406712946273 28.319420653990118 21.817714128704246 62-63 22.458648138665545 27.271518719437402 28.293669395129907 21.976163746767146 64-65 22.280247892476574 27.347679548177812 27.989496052142123 22.382576507203492 66-67 22.375521998585267 27.55451010891521 27.56016123685653 22.509806655642997 68-69 22.35980155491807 27.213751110477908 27.962542873703562 22.463904460900462 70-71 22.51027576042143 27.378289374680758 27.747487058810282 22.363947806087527 72-73 22.669707496585453 27.115373123082158 27.92035727802851 22.29456210230388 74-75 22.408107213880363 27.89984773314671 27.383543573611234 22.30850147936169 76-77 22.57620304558856 27.437838160859613 27.603496249579685 22.382462543972142 78-79 22.570018329433953 27.435102652408755 27.534555800838195 22.460323217319093 80-81 22.550812061950985 27.96132502181449 27.225521761441513 22.262341154793017 82-83 22.717298858140598 27.755220819533555 27.17630139406621 22.351178928259635 84-85 22.32954695543085 27.795645586021045 27.080952502616658 22.793854955931444 86-87 22.35660006378599 27.72655339781651 27.27422714697994 22.642619391417558 88-89 22.39270458749922 27.751347634191802 26.916134888135968 22.93981289017301 90-91 22.40582390146832 27.589823762886486 27.22165081276079 22.78270152288441 92-93 22.631340659140793 27.348468658539726 27.385428974592653 22.634761707726827 94-95 22.32124027892592 27.09684850778617 27.518558758612503 23.063352454675403 96-97 22.384056089833457 27.089625385750765 27.567931431902064 22.958387092513714 98-99 22.04417040086884 27.490663772840414 27.067242487281774 23.397923339008976 100-101 21.768969811817527 27.789501170943957 27.165098768083812 23.276430249154703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 461.0 1 337.5 2 272.0 3 341.5 4 298.5 5 293.0 6 496.5 7 948.0 8 1508.0 9 1928.5 10 1978.0 11 1933.0 12 2122.5 13 2388.0 14 2690.5 15 3018.5 16 3361.5 17 3671.0 18 3912.5 19 4265.5 20 4688.5 21 5161.0 22 5890.5 23 6794.0 24 7833.0 25 9432.5 26 11923.0 27 14860.0 28 17460.5 29 20843.0 30 25317.0 31 30112.5 32 36201.5 33 42329.5 34 48537.0 35 54035.0 36 60519.0 37 67342.5 38 70040.5 39 70007.0 40 69397.5 41 71449.0 42 74778.5 43 77594.5 44 79258.0 45 80459.5 46 79622.0 47 83382.0 48 97927.5 49 98195.0 50 86138.0 51 79508.5 52 74939.0 53 73768.5 54 72760.5 55 67767.0 56 61191.0 57 55073.0 58 50184.0 59 45089.5 60 39211.0 61 32879.5 62 27016.5 63 21333.0 64 15599.0 65 12023.0 66 9015.5 67 7055.0 68 5215.5 69 3329.5 70 2603.0 71 2131.0 72 1827.5 73 1583.5 74 1303.0 75 1112.5 76 819.0 77 495.0 78 313.0 79 211.0 80 143.5 81 101.0 82 66.5 83 46.5 84 26.5 85 14.0 86 7.5 87 2.5 88 2.5 89 3.0 90 3.0 91 2.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01216818422544311 2 0.008227597874854772 3 0.005239680751881197 4 2.5981888025857175E-4 5 0.007231625500530247 6 0.0 7 3.4642517367809565E-4 8 0.0018620353085197644 9 8.660629341952391E-5 10-11 0.0 12-13 0.0 14-15 6.711987740013104E-4 16-17 0.0039838894972981 18-19 0.001970293175294169 20-21 4.979861871622625E-4 22-23 4.330314670976196E-4 24-25 3.247736003232147E-4 26-27 1.9486416019392884E-4 28-29 2.1651573354880978E-5 30-31 6.711987740013104E-4 32-33 0.0031178265631028614 34-35 7.145019207110724E-4 36-37 0.0012341396812282157 38-39 0.0053479386186556024 40-41 6.495472006464294E-5 42-43 0.0013207459746477396 44-45 0.006582078299883817 46-47 0.0 48-49 0.0018836868818746452 50-51 0.0014506554147770256 52-53 5.845924805817864E-4 54-55 3.247736003232147E-4 56-57 0.013164156599767635 58-59 0.002165157335488098 60-61 0.0033126907232967897 62-63 1.5156101348416686E-4 64-65 0.003204432856522385 66-67 0.0011908365345184538 68-69 0.002056899468713693 70-71 0.004525178831170124 72-73 0.0027280982427150035 74-75 0.008530719901823105 76-77 0.001147533387808692 78-79 0.0075130959541437 80-81 0.00242497621574667 82-83 0.003680767470329766 84-85 0.0025115825091661936 86-87 7.145019207110724E-4 88-89 0.002987917122973575 90-91 0.008487416755113344 92-93 0.002922962402908932 94-95 6.062440539366674E-4 96-97 2.381673069036908E-4 98-99 7.361534940659533E-4 100-101 0.002295066775617384 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2309301.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.73167609097417 #Duplication Level Percentage of deduplicated Percentage of total 1 73.56536104791279 33.64267262958716 2 14.260516389690222 13.043146328466834 3 4.994584020117244 6.8523209585147224 4 2.3101991700422033 4.225971206000296 5 1.2812189370593035 2.9296144715609147 6 0.7464197347487591 2.048101532246467 7 0.49902277079039487 1.597480340106475 8 0.34749112128344883 1.2713081122419283 9 0.2529034889594483 1.0409130395433677 >10 1.4522358623303815 12.679976949403358 >50 0.1626112574218886 5.219060804830571 >100 0.11650348564120978 9.970008644280268 >500 0.008270835810762581 2.567026739880399 >1k 0.002281609878831047 1.571996830563048 >5k 2.8520123485388087E-4 0.9004701132424872 >10k+ 9.506707828462697E-5 0.4399312995317076 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10119 0.4381845415560813 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8804 0.38124090363274427 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6788 0.2939417598658642 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5120 0.22171211115398123 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2944 0.1274844639135392 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2716 0.11761134646371349 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2460 0.10652574090601442 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.495472006464294E-5 0.0 20-21 0.0 0.0 0.0 1.2990944012928587E-4 0.0 22-23 0.0 0.0 0.0 1.7321258683904785E-4 0.0 24-25 0.0 2.1651573354880978E-5 0.0 4.113798937427386E-4 0.0 26-27 0.0 4.3303146709761956E-5 0.0 7.145019207110723E-4 0.0 28-29 0.0 4.3303146709761956E-5 0.0 0.0012557912545830968 0.0 30-31 0.0 4.3303146709761956E-5 0.0 0.002858007682844289 0.0 32-33 0.0 4.3303146709761956E-5 0.0 0.004308663097621315 0.0 34-35 0.0 4.3303146709761956E-5 0.0 0.005651060645623936 0.0 36-37 0.0 4.3303146709761956E-5 0.0 0.008444113608403582 0.0 38-39 0.0 4.3303146709761956E-5 0.0 0.01327241446654204 0.0 40-41 0.0 4.3303146709761956E-5 0.0 0.018901823538811098 0.0 42-43 0.0 4.3303146709761956E-5 0.0 0.02498591565153265 0.0 44-45 0.0 4.3303146709761956E-5 0.0 0.02836356109489408 0.0 46-47 0.0 4.3303146709761956E-5 0.0 0.033343422966516705 0.0 48-49 0.0 4.3303146709761956E-5 0.0 0.040531745320337195 0.0 50-51 0.0 4.3303146709761956E-5 0.0 0.04817475071461018 0.0 52-53 0.0 4.3303146709761956E-5 0.0 0.061728635634765675 0.0 54-55 0.0 4.3303146709761956E-5 0.0 0.07196982983162437 0.0 56-57 0.0 4.3303146709761956E-5 0.0 0.09816823359103036 0.0 58-59 0.0 4.3303146709761956E-5 0.0 0.14629968115893077 0.0 60-61 0.0 4.3303146709761956E-5 0.0 0.18750262525326927 0.0 62-63 0.0 4.3303146709761956E-5 0.0 0.20872116714105265 0.0 64-65 0.0 4.3303146709761956E-5 0.0 0.24899309358113125 0.0 66-67 0.0 4.3303146709761956E-5 0.0 0.33863060727033856 0.0 68-69 0.0 4.3303146709761956E-5 0.0 0.46325706350103346 0.0 70-71 0.0 4.3303146709761956E-5 0.0 0.5716231881422127 0.0 72-73 0.0 4.3303146709761956E-5 0.0 0.7008181263507876 0.0 74-75 0.0 4.3303146709761956E-5 0.0 0.8480921283106879 0.0 76-77 0.0 4.3303146709761956E-5 0.0 0.9451777832339743 0.0 78-79 0.0 4.3303146709761956E-5 0.0 1.0727921565876426 0.0 80-81 0.0 4.3303146709761956E-5 0.0 1.2103446021112014 0.0 82-83 0.0 4.3303146709761956E-5 0.0 1.3111110245048176 0.0 84-85 0.0 4.3303146709761956E-5 0.0 1.476593999656173 0.0 86-87 0.0 4.3303146709761956E-5 0.0 1.7438393695754688 0.0 88-89 0.0 4.3303146709761956E-5 0.0 2.114925685304774 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12485 0.0 24.925068 1 TATAACG 100 0.0016531969 23.75235 2 GTATCAA 21045 0.0 23.320347 1 TACATGG 13095 0.0 23.28982 2 ACATGGG 12810 0.0 22.76765 3 GAGTACT 10960 0.0 22.731558 12-13 TACCTGG 1800 0.0 22.432775 2 GGTATCA 15910 0.0 21.7094 1 GTACTTT 11560 0.0 21.450155 14-15 GTACCTG 1980 0.0 21.115484 1 TAAGGTG 1110 0.0 20.539904 5 AGTACTT 11620 0.0 20.275417 12-13 ATCAACG 24355 0.0 20.030014 3 TCAACGC 24365 0.0 20.020927 4 CAACGCA 24470 0.0 19.954428 5 ATGGGTA 1025 0.0 19.926212 5 CATGGGA 7230 0.0 19.840326 4 TATCAAC 24700 0.0 19.751955 2 AACGCAG 24865 0.0 19.693039 6 AGAGTAC 19220 0.0 19.128334 10-11 >>END_MODULE