FastQCFastQC Report
Thu 26 May 2016
SRR936674_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936674_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269329
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81910.36094369745418137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63980.28193355833376293No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53940.23769140569745503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37970.1673181808367143No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35160.15493566600523767No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26470.11664240839472813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG123250.026.7267441
TACATGG128250.025.1249372
ACATGGG125250.024.7022173
GTATCAA172850.023.759871
GAGTACT80750.022.71262612-13
CATGGGG50400.021.8776024
GGTATCA128150.021.6246781
GTACTTT86750.021.14055614-15
CATGGGA69350.020.5597134
ATGGGTA11150.020.4619455
TCAACGC198650.020.3602544
AGTACTT86400.020.31997912-13
ATCAACG200450.020.248113
CAACGCA200650.020.1590925
GTATAGG9250.020.041151
TATCAAC203900.019.9754352
TACCTGG18350.019.9428542
AACGCAG204100.019.8178966
CCTATAC6650.019.2963333
ATGGGGA29900.019.2351135
Produced by FastQC (version 0.11.5)