##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936674_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2269329 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45915334444675 31.0 31.0 34.0 30.0 34.0 2 31.510461462397036 31.0 31.0 34.0 30.0 34.0 3 31.55268671929015 33.0 31.0 34.0 28.0 34.0 4 35.20139433286227 37.0 35.0 37.0 33.0 37.0 5 35.1256578486416 37.0 35.0 37.0 32.0 37.0 6 35.241505749056216 37.0 35.0 37.0 32.0 37.0 7 35.0929107238307 37.0 35.0 37.0 32.0 37.0 8 35.079355175031914 37.0 35.0 37.0 32.0 37.0 9 36.600224119111864 39.0 37.0 39.0 32.0 39.0 10-11 36.47455018642074 39.0 37.0 39.0 32.0 39.0 12-13 36.38627717708627 39.0 37.0 39.0 32.0 39.0 14-15 37.4801707905729 40.0 37.0 41.0 32.0 41.0 16-17 37.422491406049986 40.0 37.0 41.0 32.0 41.0 18-19 37.39433374358676 40.0 37.0 41.0 32.0 41.0 20-21 37.30822569138279 40.0 37.0 41.0 31.5 41.0 22-23 37.20365293000707 39.0 36.5 41.0 31.0 41.0 24-25 37.06144305210923 39.0 36.0 41.0 31.0 41.0 26-27 36.87560353743331 39.0 36.0 41.0 30.5 41.0 28-29 36.99570577910916 39.0 36.0 41.0 31.0 41.0 30-31 36.932153292889666 39.0 36.0 41.0 30.5 41.0 32-33 36.79177919993091 39.0 36.0 41.0 30.0 41.0 34-35 36.631459122938985 39.0 35.0 41.0 30.0 41.0 36-37 36.4009297461937 39.0 35.0 40.5 30.0 41.0 38-39 36.2290342211288 39.0 35.0 40.0 30.0 41.0 40-41 36.09213516418289 39.0 35.0 40.0 29.5 41.0 42-43 35.82509896097041 38.0 35.0 40.0 28.5 41.0 44-45 35.54713177331273 38.0 35.0 40.0 27.5 41.0 46-47 35.197960718785154 38.0 34.0 40.0 26.5 41.0 48-49 34.94776143080179 38.0 34.0 40.0 25.0 41.0 50-51 34.248515310032175 37.5 33.5 39.5 24.5 40.5 52-53 34.42928173922776 38.0 33.5 39.5 24.5 40.5 54-55 34.78247953469946 38.0 34.0 40.0 25.5 41.0 56-57 34.70918099579215 38.0 34.0 40.0 25.0 41.0 58-59 34.48195832336343 38.0 34.0 40.0 25.0 41.0 60-61 34.17388862522799 38.0 34.0 40.0 23.5 41.0 62-63 33.8573554121064 37.0 33.0 40.0 23.0 41.0 64-65 33.54905546970052 36.5 33.0 39.5 22.5 41.0 66-67 33.18354566481987 36.0 32.5 39.0 22.0 41.0 68-69 32.8051305033338 36.0 32.0 39.0 21.5 40.5 70-71 32.40284418874478 35.0 32.0 38.5 20.0 40.0 72-73 31.998272617148064 35.0 31.5 37.5 19.5 40.0 74-75 31.56828780666003 35.0 31.0 37.0 18.0 39.0 76-77 31.154901294611754 35.0 31.0 36.5 16.0 39.0 78-79 30.710640237709033 35.0 30.5 36.0 12.0 38.5 80-81 30.294465676858668 34.0 30.0 36.0 7.5 37.0 82-83 29.89631957287815 34.0 30.0 35.0 7.0 37.0 84-85 29.520688273934717 34.0 29.0 35.0 3.5 36.5 86-87 29.200618993543905 34.0 29.0 35.0 2.0 36.0 88-89 28.837699601952824 34.0 29.0 35.0 2.0 36.0 90-91 28.50265210553428 34.0 29.0 35.0 2.0 35.0 92-93 28.15180875051612 34.0 28.5 35.0 2.0 35.0 94-95 27.74551949056307 34.0 27.0 35.0 2.0 35.0 96-97 27.191478406172045 33.5 26.5 35.0 2.0 35.0 98-99 26.554910063723682 33.0 25.0 35.0 2.0 35.0 100-101 24.441835009379425 31.0 19.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26063602233720573 1101 2 0.266595973430654 1101 3 0.28449952327661876 1101 4 0.3491996862901132 1101 5 0.35204706526002383 1101 6 0.2106376665354972 1101 7 0.23293708029917326 1101 8 0.19426620444150444 1101 9 0.33628704982645274 1101 10-11 0.22449408691797856 1101 12-13 0.4566128967498244 1101 14-15 0.4631754795638088 1101 16-17 0.5337916396031588 1101 18-19 0.5507665719428374 1101 20-21 0.5524988977916152 1101 22-23 0.500003097188042 1101 24-25 0.45677300564565115 1101 26-27 0.4263665822627729 1101 28-29 0.48016334131362726 1101 30-31 0.3315679214182339 1101 32-33 0.17530510897188378 1101 34-35 0.2831092574745142 1101 36-37 0.38128616676068106 1101 38-39 0.24195489031082928 1101 40-41 0.2597469303616293 1101 42-43 0.07034461564698091 1101 44-45 0.10387858040654407 1101 46-47 0.25514661392913496 1101 48-49 0.18361728853568593 1101 50-51 0.2971760323784096 1101 52-53 0.2662350182494677 1101 54-55 0.264848873759135 1101 56-57 0.2708984131439749 1101 58-59 0.2650854154120026 1101 60-61 0.3608878737794967 1101 62-63 0.4175926566226096 1101 64-65 0.4787876446541972 1101 66-67 0.41970369714675115 1101 68-69 0.4507955342642802 1101 70-71 0.33972545308908764 1101 72-73 0.35649190658397245 1101 74-75 0.2705311308640823 1101 76-77 0.17483043118382469 1101 78-79 0.32530205941013435 1101 80-81 0.10378732256958756 1101 82-83 0.21990952141462472 1101 84-85 0.2057606216297181 1101 86-87 0.040675545178398664 1101 88-89 -0.030913391074239627 1101 90-91 -0.06287918072940357 1101 92-93 -0.3904436187657119 1101 94-95 -0.2509378340693367 1101 96-97 -0.24760878990870694 1101 98-99 -0.34954734504939466 1101 100-101 -0.13817437108102482 1102 1 0.15545492162952002 1102 2 0.2687928551433494 1102 3 0.28998644590641476 1102 4 0.221448231123631 1102 5 0.3163079822918391 1102 6 0.38338422832282504 1102 7 0.23341328545794937 1102 8 0.17508667052872795 1102 9 0.532819602503082 1102 10-11 0.4339304290228583 1102 12-13 0.5581345263992503 1102 14-15 0.4193391959282522 1102 16-17 0.35745669493774557 1102 18-19 0.40976016886771305 1102 20-21 0.25051545191626445 1102 22-23 -0.04338167658025327 1102 24-25 0.08429092614794342 1102 26-27 0.15065735633025668 1102 28-29 0.2750204112472616 1102 30-31 0.14501183826847353 1102 32-33 0.1608669282811448 1102 34-35 0.1199454680234382 1102 36-37 0.008621085160591235 1102 38-39 0.08376556050392736 1102 40-41 -0.10945167459707505 1102 42-43 0.15469545208934932 1102 44-45 0.11200604351206778 1102 46-47 0.3167353862219855 1102 48-49 0.2731978168835951 1102 50-51 0.2111145116966 1102 52-53 0.026215484702127867 1102 54-55 -0.11456601627401852 1102 56-57 0.14618833301132383 1102 58-59 0.15316264287019976 1102 60-61 -0.13422559894376462 1102 62-63 -0.018062833497566544 1102 64-65 0.17271477270314506 1102 66-67 0.11346024211273686 1102 68-69 0.06752689402986078 1102 70-71 0.1164324829963661 1102 72-73 0.10383095323723168 1102 74-75 -0.0370938330208368 1102 76-77 -0.019696235734080858 1102 78-79 0.05630581309444338 1102 80-81 0.08811509104023685 1102 82-83 0.028081493143798042 1102 84-85 -0.031297047157604396 1102 86-87 -0.038037484685489176 1102 88-89 -0.17242269306253988 1102 90-91 -0.2854591278101033 1102 92-93 -0.29047950544944 1102 94-95 -0.08966987217031885 1102 96-97 -0.18653276609616398 1102 98-99 -0.09294884880707599 1102 100-101 0.1535673806916158 1103 1 0.28802320958362415 1103 2 0.3499617478380195 1103 3 0.3691413132626238 1103 4 0.26854598567191346 1103 5 0.4408568844378067 1103 6 0.11577823587202118 1103 7 0.2979100633727185 1103 8 0.17007381416586753 1103 9 0.2561463507256363 1103 10-11 0.376939019276179 1103 12-13 0.5390223082946974 1103 14-15 0.6259639239667294 1103 16-17 0.6315192937588208 1103 18-19 0.5453216620771144 1103 20-21 0.46804440093969646 1103 22-23 0.4170408897416422 1103 24-25 0.445942012395129 1103 26-27 0.4998797862124462 1103 28-29 0.44149358106614045 1103 30-31 0.25937528545552624 1103 32-33 0.3539295555271593 1103 34-35 0.37868628469561116 1103 36-37 0.45387198854066213 1103 38-39 0.45140527796004193 1103 40-41 0.4486305421691341 1103 42-43 0.4211961289582007 1103 44-45 0.4087342738583004 1103 46-47 0.516689580617772 1103 48-49 0.5441271584080454 1103 50-51 0.1477831562652412 1103 52-53 0.32748030564277286 1103 54-55 0.5423152172261965 1103 56-57 0.5864696096226965 1103 58-59 0.6379786920642943 1103 60-61 0.7489627079839849 1103 62-63 0.5066464326771083 1103 64-65 0.5118260669971946 1103 66-67 0.7188852304762747 1103 68-69 0.6013679678292903 1103 70-71 0.7986130962407358 1103 72-73 0.5965800770977232 1103 74-75 0.41330775806510545 1103 76-77 0.36833970760080703 1103 78-79 0.6281948516090488 1103 80-81 0.6358840886703092 1103 82-83 0.5247141742432255 1103 84-85 0.2321793346793335 1103 86-87 0.343390992839268 1103 88-89 0.4464496600130978 1103 90-91 0.528749551508028 1103 92-93 0.37184895300692133 1103 94-95 0.3788338153732802 1103 96-97 0.5709854821222429 1103 98-99 0.5191112900724377 1103 100-101 0.5806181807944171 1104 1 0.2029446862949591 1104 2 0.24135559540024687 1104 3 0.07037116947424948 1104 4 0.1688070460125246 1104 5 0.14358460197493628 1104 6 0.17247820588322327 1104 7 0.23965762086439213 1104 8 0.3070796698724152 1104 9 0.2971009413786021 1104 10-11 0.4409551500904527 1104 12-13 0.35146702632111726 1104 14-15 0.3256297856710404 1104 16-17 0.033400064628651194 1104 18-19 0.20578917158672283 1104 20-21 0.15487711082178635 1104 22-23 0.24601779837053073 1104 24-25 0.19363492714803243 1104 26-27 0.19548390256896653 1104 28-29 0.059619123626546866 1104 30-31 0.01542797103092397 1104 32-33 -0.00369959358027927 1104 34-35 0.04063318874725752 1104 36-37 0.24130321160993873 1104 38-39 0.07365633151371043 1104 40-41 0.08245569171851486 1104 42-43 0.13611871051983115 1104 44-45 0.10775095709772131 1104 46-47 0.19761781269946965 1104 48-49 0.09567773687609105 1104 50-51 -0.20292836732330954 1104 52-53 0.055657776166945894 1104 54-55 0.3014240765471854 1104 56-57 0.2404884236448268 1104 58-59 0.3120159728698866 1104 60-61 0.15528529270232383 1104 62-63 0.09825588388218875 1104 64-65 0.1470251768035311 1104 66-67 0.2067553947832934 1104 68-69 0.19566046905251966 1104 70-71 0.2578655860997898 1104 72-73 0.05700796983253653 1104 74-75 -0.15596919633614803 1104 76-77 -0.2332806342714413 1104 78-79 0.06494974846338053 1104 80-81 0.09409565171780088 1104 82-83 -0.015838093067408465 1104 84-85 0.0873165728692733 1104 86-87 0.16412256156884908 1104 88-89 -0.012023436882270033 1104 90-91 -0.12134499210988281 1104 92-93 -0.3519616756528947 1104 94-95 -0.20404684501152204 1104 96-97 0.14542382358411743 1104 98-99 0.05647937883926346 1104 100-101 0.0958946301212471 1105 1 0.18488776369455096 1105 2 0.2268396439943885 1105 3 0.19556234543873074 1105 4 0.3118417708636798 1105 5 0.3167624937049851 1105 6 0.23464601398454477 1105 7 0.38715047972800676 1105 8 0.05008376527111835 1105 9 0.32921850341184467 1105 10-11 0.26469400819693334 1105 12-13 0.24358648118569448 1105 14-15 0.28860841388455327 1105 16-17 0.3630380176176047 1105 18-19 0.20211140030957608 1105 20-21 -0.05227727538463256 1105 22-23 -0.1135810934627699 1105 24-25 -0.07946036830572467 1105 26-27 0.04255982231292421 1105 28-29 0.08415434749687734 1105 30-31 0.039510636137435995 1105 32-33 -0.01089028773315448 1105 34-35 0.02803828906731809 1105 36-37 -0.09642625117010795 1105 38-39 -0.15785277128423303 1105 40-41 -0.31865281913945154 1105 42-43 -0.2540316835332419 1105 44-45 -0.14006282271436987 1105 46-47 -0.050083085535042926 1105 48-49 0.04320019273870912 1105 50-51 0.21734144717921566 1105 52-53 0.13513036452776106 1105 54-55 -0.011378690428067273 1105 56-57 0.19455990383892896 1105 58-59 0.12993058896927323 1105 60-61 0.23406131913314 1105 62-63 0.11776151075351038 1105 64-65 0.010777973031629529 1105 66-67 -0.2674587360629701 1105 68-69 -0.10955307673679471 1105 70-71 0.192051496551656 1105 72-73 0.1126974766639357 1105 74-75 0.07630030843034419 1105 76-77 0.2849416529571087 1105 78-79 0.24449839422860364 1105 80-81 0.29247607640032314 1105 82-83 0.19619842136973276 1105 84-85 0.0775364854548819 1105 86-87 0.12992804733820051 1105 88-89 0.30636731233478187 1105 90-91 0.39506809251688324 1105 92-93 0.36180247756319517 1105 94-95 0.32050214490484663 1105 96-97 0.20791083187815929 1105 98-99 -0.09444053718785383 1105 100-101 0.148829551138558 1106 1 -0.04342353841428093 1106 2 -0.02898892950648957 1106 3 -0.07670804931003872 1106 4 0.0847254133594646 1106 5 0.19729976545968242 1106 6 0.22607487035546825 1106 7 0.3387622949921081 1106 8 0.2845911906097456 1106 9 0.3452115404569369 1106 10-11 0.33184608711042785 1106 12-13 0.3029526528297808 1106 14-15 0.22706713480423701 1106 16-17 0.24699892352705177 1106 18-19 0.2585409819882969 1106 20-21 0.2586142185681126 1106 22-23 0.33738492586668656 1106 24-25 0.15939310138546858 1106 26-27 0.1317094895779647 1106 28-29 0.011238610130831717 1106 30-31 0.14530947201841116 1106 32-33 -0.03107505989583359 1106 34-35 -0.08486384877743802 1106 36-37 0.07462118396016848 1106 38-39 0.01779501924686855 1106 40-41 -0.01155133044517953 1106 42-43 0.061486276901213444 1106 44-45 0.010638821921062913 1106 46-47 0.09193644337754137 1106 48-49 -0.01540630964574774 1106 50-51 0.1484694821390562 1106 52-53 0.11939707833943203 1106 54-55 0.19886537564747186 1106 56-57 0.14425844778350694 1106 58-59 0.21020557128989736 1106 60-61 0.30303600589082436 1106 62-63 0.2456554888854825 1106 64-65 0.08798852988669381 1106 66-67 0.2167444226525106 1106 68-69 0.0784737233865016 1106 70-71 0.10192746891743099 1106 72-73 0.27857917896134765 1106 74-75 0.22552740230331025 1106 76-77 0.06165682458306776 1106 78-79 0.12325784589590327 1106 80-81 0.24259466422603282 1106 82-83 0.3408000460592113 1106 84-85 0.26838745283416543 1106 86-87 0.2859219910180464 1106 88-89 0.2450118933788623 1106 90-91 0.27351566182911213 1106 92-93 0.2501120570659978 1106 94-95 0.1714633590701098 1106 96-97 0.16779160940812687 1106 98-99 0.3969687358723988 1106 100-101 0.5404063703011879 1107 1 0.45613561642342404 1107 2 0.48296767840423627 1107 3 0.520642865646888 1107 4 0.47608332283216726 1107 5 0.4828456036224438 1107 6 0.4748690941097209 1107 7 0.6689975696297097 1107 8 0.6239225470139402 1107 9 0.7794234532111446 1107 10-11 0.8007038335115553 1107 12-13 0.8305249938851844 1107 14-15 0.8394457217514955 1107 16-17 0.7845527496877409 1107 18-19 0.9932177239689466 1107 20-21 1.0543132436510305 1107 22-23 0.938350309536446 1107 24-25 0.9269043282355796 1107 26-27 1.0820948904078733 1107 28-29 0.9526707297099506 1107 30-31 0.8913483382445335 1107 32-33 0.9859040518909765 1107 34-35 0.7303255902061991 1107 36-37 0.8650756267822644 1107 38-39 0.9310043178384362 1107 40-41 0.7328360473138602 1107 42-43 0.8133440383551189 1107 44-45 0.9378089532313112 1107 46-47 1.0843104141450866 1107 48-49 1.115408897655847 1107 50-51 1.018826182373381 1107 52-53 1.0769121687312548 1107 54-55 1.150059310388734 1107 56-57 1.1058172112494589 1107 58-59 1.15305295135704 1107 60-61 1.0909764371022277 1107 62-63 1.1790590684317905 1107 64-65 1.017840360553187 1107 66-67 1.0455829015209375 1107 68-69 1.1044109333072782 1107 70-71 1.3164368242077202 1107 72-73 1.2167727474187977 1107 74-75 1.1158524917417871 1107 76-77 1.151966158799496 1107 78-79 1.1444993975343998 1107 80-81 1.3087774348370225 1107 82-83 1.3407811799334404 1107 84-85 1.2252199507393193 1107 86-87 1.3085906446490263 1107 88-89 1.5039015014551715 1107 90-91 1.459954730765734 1107 92-93 1.449998860311375 1107 94-95 1.4485888840351144 1107 96-97 1.5402052191101276 1107 98-99 1.6912653454891071 1107 100-101 1.9113248395358546 1108 1 0.16367989289442164 1108 2 0.08595397849507336 1108 3 0.00907379992077395 1108 4 0.06948988173093085 1108 5 0.24028157416892526 1108 6 0.2764003453985211 1108 7 0.39581898177483765 1108 8 0.13793783817338579 1108 9 0.3261953463007288 1108 10-11 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50-51 -2.1124931394687323 2316 52-53 -2.213117002561752 2316 54-55 -2.1529011374093656 2316 56-57 -1.771051332042397 2316 58-59 -1.762718135827356 2316 60-61 -2.0147929751639637 2316 62-63 -2.00713508971155 2316 64-65 -1.8752065545987087 2316 66-67 -2.5607978200510217 2316 68-69 -2.1013931480932655 2316 70-71 -1.9154816452658778 2316 72-73 -1.651589791852782 2316 74-75 -1.6872734169491217 2316 76-77 -2.407201342339615 2316 78-79 -2.3822516267103424 2316 80-81 -2.5090592016213833 2316 82-83 -2.2629992370949132 2316 84-85 -2.506205481113607 2316 86-87 -2.5556141072922465 2316 88-89 -2.7963705951003988 2316 90-91 -2.8902631400142056 2316 92-93 -2.997312418100414 2316 94-95 -3.012366646050218 2316 96-97 -3.286883249782864 2316 98-99 -3.905673353221303 2316 100-101 -4.7491686424996296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4420.0 3 1732.0 4 1703.0 5 1899.0 6 2106.0 7 2570.0 8 3128.0 9 3883.0 10 5019.0 11 6272.0 12 8220.0 13 10005.0 14 11424.0 15 14414.0 16 18029.0 17 22381.0 18 19389.0 19 13334.0 20 13413.0 21 14474.0 22 15870.0 23 17666.0 24 20697.0 25 24436.0 26 28447.0 27 34253.0 28 40700.0 29 49555.0 30 60685.0 31 75273.0 32 94815.0 33 122282.0 34 162262.0 35 223524.0 36 315340.0 37 417916.0 38 337312.0 39 50468.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80030850594976 18.54486557955046 14.110973997355664 22.543851917144117 2 15.456800177685645 20.050714444362182 40.079967353788334 24.412518024163838 3 19.489170308294096 24.855583544721068 29.460075482607444 26.195170664377393 4 12.553492366042068 16.305363607029694 37.43134374939389 33.70980027753435 5 13.646347119330315 37.70374360972926 33.76121769154941 14.888691579391015 6 31.700844103620508 35.89583274543556 17.678688588198778 14.724634562745154 7 29.110522822059604 32.75604898960915 20.402022985587507 17.73140520274374 8 26.90665994345805 33.62412385983873 19.74622238246653 19.722993814236688 9 26.907151567717747 16.793132125664226 20.553909455226734 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22.65131968880066 26.477921459843078 28.918558048019534 21.952200803336734 36-37 22.456716335692843 26.12223538160685 29.094620872008154 22.326427410692155 38-39 22.00910370502903 26.614389477107654 29.190445384380126 22.186061433483196 40-41 22.2027492832421 26.422609095708445 29.312886306729197 22.061755314320266 42-43 22.02377765453944 26.605842158130944 28.7204225238844 22.64995766344521 44-45 22.181130183105243 26.647823818488202 28.59239164921632 22.578654349190234 46-47 22.478144877056945 26.947615568409468 28.185969711738885 22.388269842794706 48-49 22.55883746852311 27.02371433601247 27.927023185544066 22.490425009920358 50-51 22.16855157783689 27.16114068100931 28.09176071759779 22.57854702355601 52-53 22.625384505727403 27.327365438694017 27.61597745922273 22.431272596355853 54-55 22.16563785101504 26.99662799941479 27.90839457166074 22.92933957790943 56-57 22.418171318202322 27.37831596393287 27.586598941465056 22.616913776399752 58-59 22.76473584804223 26.841501774585748 27.701252653946522 22.692509723425502 60-61 22.521598520784178 27.088734661916032 28.256181778562254 22.13348503873754 62-63 22.286953563531213 27.18982203275556 28.158096747270296 22.36512765644293 64-65 22.023495412474116 27.314226739521818 27.887852007589288 22.77442584041478 66-67 22.08860551708365 27.48979237949984 27.457535175005248 22.96406692841126 68-69 22.061865574229962 27.207781848757566 27.984627032343873 22.7457255446686 70-71 22.259549829932425 27.164847000878762 27.733354839676384 22.842248329512433 72-73 22.29501947788687 27.017834164742382 27.98472175706404 22.70242460030671 74-75 21.96099868811686 27.686176576738553 27.63889237754913 22.71393235759546 76-77 22.204632006818102 27.329267680039344 27.70917226542291 22.756928047719647 78-79 22.184092046684558 27.33611793817578 27.674550796777403 22.805239218362257 80-81 22.237489644904233 27.761816158731634 27.35038911359058 22.65030508277355 82-83 22.29242467876225 27.602727003713373 27.491477837971946 22.61337047955243 84-85 22.065095408320996 27.555913306308117 27.313109447271415 23.065881838099468 86-87 22.1757117501435 27.57936092598856 27.47493822005449 22.769989103813444 88-89 22.093735781041534 27.50637615280292 27.19269697767603 23.20719108847952 90-91 22.074645602732623 27.291878440486755 27.616070049362534 23.017405907418087 92-93 22.393640745206717 27.088688096295826 27.753075352753275 22.764595805744182 94-95 21.993879724781518 26.824437384622367 27.84599628323724 23.335686607358873 96-97 21.97190453579467 26.788918255221216 28.039101412264746 23.200075796719368 98-99 21.70651526908412 27.157440894845397 27.563996471288004 23.572047364782474 100-101 21.462270285234908 26.997598759660395 27.956000918704767 23.58413003639993 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 260.0 1 197.0 2 184.5 3 261.0 4 253.5 5 251.5 6 444.0 7 864.5 8 1448.0 9 1838.0 10 1794.0 11 1746.5 12 1902.0 13 2066.0 14 2315.5 15 2522.5 16 2625.0 17 2823.5 18 3070.5 19 3292.5 20 3640.0 21 4187.0 22 4963.0 23 5789.0 24 6783.0 25 8292.5 26 10592.0 27 13560.5 28 16216.0 29 19446.0 30 23749.0 31 28882.5 32 35705.5 33 41686.0 34 47700.0 35 53516.0 36 59921.5 37 66376.5 38 68727.5 39 68638.0 40 67903.0 41 70236.0 42 73682.0 43 76743.5 44 78964.5 45 80625.0 46 79985.5 47 82658.5 48 96481.0 49 97404.0 50 85668.0 51 79283.5 52 74762.0 53 73696.0 54 72865.0 55 67847.0 56 61332.0 57 54864.0 58 49816.0 59 44565.0 60 38340.5 61 32171.0 62 26682.0 63 20820.0 64 15517.5 65 12158.0 66 8784.0 67 6799.5 68 5052.0 69 3246.5 70 2541.0 71 2089.0 72 1713.5 73 1510.0 74 1217.5 75 958.5 76 692.0 77 416.5 78 269.0 79 175.5 80 112.0 81 76.5 82 57.0 83 39.0 84 24.5 85 15.5 86 12.5 87 10.0 88 10.5 89 6.0 90 5.0 91 6.5 92 5.0 93 3.0 94 3.5 95 2.5 96 1.0 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014497677507316039 2 0.0067420810292381575 3 0.008680980148757629 4 0.0344154593714706 5 0.04569632697594752 6 0.05997367503786362 7 0.026571731115232745 8 0.025161622664673124 9 0.014541743396396026 10-11 0.04492517391704773 12-13 0.04166429812512862 14-15 0.01958728769605465 16-17 0.022605801098033822 18-19 0.02952414568359193 20-21 0.023178657656073666 22-23 0.01394685389381619 24-25 0.0030625792910591635 26-27 0.0028642827901992174 28-29 0.0012779107833196508 30-31 0.00680817986285814 32-33 0.0013880755060196209 34-35 0.0019388991195194703 36-37 0.0062353233048182966 38-39 0.006301422138438278 40-41 0.003657468793639001 42-43 5.948895025798375E-4 44-45 0.0014541743396396026 46-47 0.0029524145683591935 48-49 2.4236238993993379E-4 50-51 0.0015643390623395726 52-53 8.592848370597653E-4 54-55 0.002335492121239362 56-57 0.0026659862893392717 58-59 0.0029524145683591935 60-61 8.152189479797772E-4 62-63 0.0015423061177995787 64-65 0.0034151064036990666 66-67 0.0030185134019791757 68-69 0.006653949251078183 70-71 0.010179220377477219 72-73 0.003921864128118928 74-75 0.0031727440137591335 76-77 0.003304941680999097 78-79 0.03456968998325055 80-81 0.02284816348797376 82-83 0.0043404900743788145 84-85 0.009408067318577431 86-87 0.008218288313417754 88-89 0.0057946644140184165 90-91 0.013241799668536381 92-93 0.014145150394676136 94-95 0.006609883361998194 96-97 0.004472687741618778 98-99 0.02258376815349383 100-101 0.040452486175428945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2269329.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.56802166347955 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48692749509894 36.662507298914754 2 13.467719745717286 13.082010087353368 3 4.62765862976837 6.74268673745335 4 2.0986204531363812 4.077033745253962 5 1.1182410132230214 2.715537687760351 6 0.6417122594425648 1.870001695099614 7 0.43657545022029576 1.4842524146829863 8 0.31872918202730005 1.2384036653988022 9 0.2283375924708615 0.998091462394041 >10 1.306885747614713 12.115872834802019 >50 0.15662161241497294 5.322203885646369 >100 0.10394349468684937 9.422662675823105 >500 0.005838053947928726 1.9202488395422517 >1k 0.0019156114516641065 1.463169097079626 >5k 2.736587788091581E-4 0.8853178727954389 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8191 0.36094369745418137 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6398 0.28193355833376293 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5394 0.23769140569745503 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3797 0.1673181808367143 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3516 0.15493566600523767 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2647 0.11664240839472813 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.3219766723996388E-4 0.0 24-25 0.0 0.0 0.0 4.186259462598856E-4 0.0 26-27 0.0 0.0 0.0 7.931860034397834E-4 0.0 28-29 0.0 0.0 0.0 0.0015423061177995785 0.0 30-31 0.0 0.0 0.0 0.0028642827901992174 0.0 32-33 0.0 0.0 0.0 0.0045828524643187485 0.0 34-35 0.0 0.0 0.0 0.005948895025798375 0.0 36-37 0.0 0.0 0.0 0.008548782481517664 0.0 38-39 0.0 0.0 0.0 0.013197733779456395 0.0 40-41 0.0 0.0 0.0 0.018860200526234848 0.0 42-43 0.0 0.0 0.0 0.02419217310491339 0.0 44-45 0.0 0.0 0.0 0.027453048896832498 0.0 46-47 0.0 0.0 0.0 0.03199183547207126 0.0 48-49 0.0 0.0 0.0 0.03853562000044947 0.0 50-51 0.0 0.0 0.0 0.04521160219606765 0.0 52-53 0.0 0.0 0.0 0.05763818291662425 0.0 54-55 0.0 0.0 0.0 0.06706828317974167 0.0 56-57 0.0 0.0 0.0 0.09247226823435473 0.0 58-59 0.0 0.0 0.0 0.13966683543902186 0.0 60-61 0.0 0.0 0.0 0.17716690704609162 0.0 62-63 0.0 0.0 0.0 0.19574067929330652 0.0 64-65 0.0 0.0 0.0 0.2335492121239362 0.0 66-67 0.0 0.0 0.0 0.32154879261667213 0.0 68-69 0.0 0.0 0.0 0.4388081234585201 0.0 70-71 0.0 0.0 0.0 0.5374275832195332 0.0 72-73 0.0 0.0 0.0 0.6529683443872616 0.0 74-75 0.0 0.0 0.0 0.7880523273619646 0.0 76-77 0.0 0.0 0.0 0.8768671268026804 0.0 78-79 0.0 0.0 0.0 1.0006261762838267 0.0 80-81 0.0 0.0 0.0 1.1214989100302337 0.0 82-83 0.0 0.0 0.0 1.2168574939993277 0.0 84-85 0.0 0.0 0.0 1.3711101387238256 0.0 86-87 0.0 0.0 0.0 1.632971684581654 0.0 88-89 0.0 0.0 0.0 2.0027505927963727 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12325 0.0 26.726744 1 TACATGG 12825 0.0 25.124937 2 ACATGGG 12525 0.0 24.702217 3 GTATCAA 17285 0.0 23.75987 1 GAGTACT 8075 0.0 22.712626 12-13 CATGGGG 5040 0.0 21.877602 4 GGTATCA 12815 0.0 21.624678 1 GTACTTT 8675 0.0 21.140556 14-15 CATGGGA 6935 0.0 20.559713 4 ATGGGTA 1115 0.0 20.461945 5 TCAACGC 19865 0.0 20.360254 4 AGTACTT 8640 0.0 20.319979 12-13 ATCAACG 20045 0.0 20.24811 3 CAACGCA 20065 0.0 20.159092 5 GTATAGG 925 0.0 20.04115 1 TATCAAC 20390 0.0 19.975435 2 TACCTGG 1835 0.0 19.942854 2 AACGCAG 20410 0.0 19.817896 6 CCTATAC 665 0.0 19.296333 3 ATGGGGA 2990 0.0 19.235113 5 >>END_MODULE