FastQCFastQC Report
Thu 26 May 2016
SRR936674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269329
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105300.4640138120122732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88160.38848487812917387No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66520.2931262941600799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52510.23138998355901677No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28770.12677756288312536No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26010.11461537749704868No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23260.102497258000052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG121000.024.3518071
CCTATAC5450.023.5326463
GTATCAA201950.023.4304811
TACATGG126100.023.0556872
TAAGGTG8300.022.8860325
CATGGGG45950.022.8439884
ACATGGG122950.022.7170683
TACCTGG17150.022.7138882
GAGTACT112450.021.98136712-13
CCCTATA5250.021.7166942
GGTATCA149750.021.4070991
GTACTTT118400.020.85644314-15
GTAAGGT7750.020.8373474
AGTACTT118150.020.41848212-13
GTACCTG19450.019.9854341
ATCAACG237850.019.7912853
TCAACGC237800.019.773734
TATCAAC239700.019.739362
CAACGCA239250.019.670275
AACGCAG243800.019.362036
Produced by FastQC (version 0.11.5)