FastQCFastQC Report
Thu 26 May 2016
SRR936673_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936673_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336386
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83090.355634728165637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66660.2853124440910021No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54630.23382266457682935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37980.1625587552741713No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36280.15528256033035637No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26590.11380824915061125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131700.026.9962641
TACATGG135900.025.5323752
ACATGGG134250.024.7486043
GTATCAA175400.024.1183871
GAGTACT83750.022.32920812-13
GGTATCA129900.022.174371
GTACTTT88700.021.13622714-15
ATCAACG199800.020.93513
TCAACGC200050.020.8856244
CAACGCA201350.020.7026775
TATCAAC204050.020.5456472
CATGGGG56200.020.4680374
AACGCAG204750.020.4048866
CATGGGA71600.019.65064
GGACCGA2702.2850145E-819.3641366
AGTACTT88500.019.27811812-13
ATGGGTA11350.019.2637355
GTATAGG9350.018.809531
AGAGTAC161200.018.4396410-11
ACGCAGA226400.018.369657
Produced by FastQC (version 0.11.5)