##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936673_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2336386 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.454708254543554 31.0 31.0 34.0 30.0 34.0 2 31.493304616617287 31.0 31.0 34.0 28.0 34.0 3 31.544597938867977 33.0 31.0 34.0 28.0 34.0 4 35.170734630322215 37.0 35.0 37.0 33.0 37.0 5 35.14597887506602 37.0 35.0 37.0 32.0 37.0 6 35.27521522556632 37.0 35.0 37.0 32.0 37.0 7 35.147807768065725 37.0 35.0 37.0 32.0 37.0 8 35.09526251227323 37.0 35.0 37.0 32.0 37.0 9 36.59206526661262 39.0 37.0 39.0 32.0 39.0 10-11 36.498186729418855 39.0 37.0 39.0 32.0 39.0 12-13 36.396243386152804 39.0 37.0 39.0 32.0 39.0 14-15 37.461805326688314 40.0 37.0 41.0 32.0 41.0 16-17 37.344144332314954 40.0 37.0 41.0 32.0 41.0 18-19 37.30440667766371 39.5 37.0 41.0 32.0 41.0 20-21 37.22520636572895 39.5 36.5 41.0 31.0 41.0 22-23 37.093491400821605 39.0 36.0 41.0 31.0 41.0 24-25 37.2357900192862 39.0 37.0 41.0 31.0 41.0 26-27 37.22499086195518 39.0 37.0 41.0 31.0 41.0 28-29 37.13252733067225 39.0 36.0 41.0 31.0 41.0 30-31 36.957071947871626 39.0 36.0 41.0 30.5 41.0 32-33 36.78435391241002 39.0 36.0 41.0 30.0 41.0 34-35 36.571180875078 39.0 35.0 41.0 30.0 41.0 36-37 36.387636717562934 39.0 35.0 40.0 30.0 41.0 38-39 36.19330624306086 39.0 35.0 40.0 30.0 41.0 40-41 36.01942658447705 38.5 35.0 40.0 29.0 41.0 42-43 35.78423920533679 38.0 35.0 40.0 28.5 41.0 44-45 35.51151265244698 38.0 35.0 40.0 27.5 41.0 46-47 35.21349083584647 38.0 34.0 40.0 26.0 41.0 48-49 35.103936592669186 38.0 34.0 40.0 26.0 41.0 50-51 34.18822959904742 37.5 33.5 39.5 24.5 40.5 52-53 34.3326089952602 38.0 33.5 39.5 24.5 40.5 54-55 34.74656285391198 38.0 34.0 40.0 25.0 41.0 56-57 34.659565457077726 38.0 34.0 40.0 25.0 41.0 58-59 34.40593784588677 38.0 34.0 40.0 24.0 41.0 60-61 34.09604448922396 37.5 33.5 40.0 23.0 41.0 62-63 33.77351559202974 37.0 33.0 40.0 22.5 41.0 64-65 33.431338400418426 36.5 33.0 39.5 22.0 41.0 66-67 33.04733999433313 36.0 32.0 39.0 21.5 41.0 68-69 32.640203502332234 35.5 32.0 39.0 20.5 40.5 70-71 32.19170526616749 35.0 31.5 38.5 18.5 40.0 72-73 31.797537307619546 35.0 31.0 37.5 18.0 40.0 74-75 31.377789243729417 35.0 31.0 37.0 16.0 39.0 76-77 30.949416962779267 35.0 31.0 36.5 12.5 39.0 78-79 30.502524625639772 34.5 30.0 36.0 9.0 38.5 80-81 30.025291839618966 34.0 30.0 35.5 7.0 37.0 82-83 29.552834591544375 34.0 29.0 35.0 4.0 37.0 84-85 29.24098629250475 34.0 29.0 35.0 2.0 36.5 86-87 28.93144797135405 34.0 29.0 35.0 2.0 36.0 88-89 28.535926212535088 34.0 29.0 35.0 2.0 36.0 90-91 28.137588352267137 34.0 27.5 35.0 2.0 35.0 92-93 27.770974059937014 34.0 27.0 35.0 2.0 35.0 94-95 27.32184343682936 33.0 26.0 35.0 2.0 35.0 96-97 26.762151459561906 33.0 25.0 35.0 2.0 35.0 98-99 26.060713426634127 33.0 24.5 35.0 2.0 35.0 100-101 23.524981317299453 30.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28339601710763773 1101 2 0.4193344979835203 1101 3 0.4270243670706826 1101 4 0.40959382225151586 1101 5 0.3770904026085944 1101 6 0.20631830768685688 1101 7 0.3089199668774256 1101 8 0.38748502517368166 1101 9 0.6521407275213988 1101 10-11 0.5269829678475233 1101 12-13 0.6088032849208673 1101 14-15 0.8439939032439625 1101 16-17 0.7425010516356778 1101 18-19 0.7234449327578787 1101 20-21 0.6943726860400901 1101 22-23 0.6797205293694972 1101 24-25 0.6053772877422574 1101 26-27 0.5896033456138667 1101 28-29 0.5407722940863522 1101 30-31 0.5637081918354809 1101 32-33 0.5561730838750378 1101 34-35 0.5806240296329435 1101 36-37 0.5476663890092013 1101 38-39 0.4269179180655698 1101 40-41 0.4684947227624221 1101 42-43 0.3383136898272383 1101 44-45 0.4857987134240602 1101 46-47 0.6837657061826832 1101 48-49 0.8004294474078364 1101 50-51 0.6967634602465225 1101 52-53 0.7387394718212548 1101 54-55 0.6866842889711222 1101 56-57 0.5505309150259663 1101 58-59 0.5031042707044264 1101 60-61 0.4580081895955743 1101 62-63 0.5081139260668479 1101 64-65 0.48141816012269345 1101 66-67 0.4975335041630089 1101 68-69 0.4900656682057374 1101 70-71 0.41028035627456205 1101 72-73 0.46116740645642196 1101 74-75 0.42911526612325446 1101 76-77 0.4058422112167399 1101 78-79 0.37854018626131136 1101 80-81 0.5369976225256003 1101 82-83 0.6839083192323407 1101 84-85 0.6547524782363361 1101 86-87 0.5226710496494924 1101 88-89 0.47334154272173734 1101 90-91 0.3080029540689715 1101 92-93 0.2516616349029164 1101 94-95 0.3565345422400803 1101 96-97 0.390899490644923 1101 98-99 0.4063897378078174 1101 100-101 0.7742076300561394 1102 1 0.2756597650798369 1102 2 0.3927373319418166 1102 3 0.304193162933899 1102 4 0.3270511320468614 1102 5 0.3674111096871542 1102 6 0.3693435812154888 1102 7 0.2833146098926136 1102 8 0.27009060300036936 1102 9 0.4345332961499224 1102 10-11 0.4563269996170831 1102 12-13 0.37778677889946266 1102 14-15 0.3912810686550898 1102 16-17 0.43841034472394114 1102 18-19 0.5368500203778197 1102 20-21 0.5779567828236907 1102 22-23 0.3822173291416959 1102 24-25 0.23572559864123832 1102 26-27 0.282976067598824 1102 28-29 0.3797119052858875 1102 30-31 0.18219261428307476 1102 32-33 0.29515905490205085 1102 34-35 0.09850296078089116 1102 36-37 0.3142655118060489 1102 38-39 0.35005497830110954 1102 40-41 0.19436859632041603 1102 42-43 0.12166003520525948 1102 44-45 -0.12105468008986975 1102 46-47 -0.014691610116322806 1102 48-49 -0.0766215755481241 1102 50-51 0.0980016875507772 1102 52-53 0.13825125858470955 1102 54-55 0.221595826389688 1102 56-57 0.22333996428331915 1102 58-59 0.35486472824581483 1102 60-61 0.16855009483603567 1102 62-63 0.21044198398453062 1102 64-65 0.13899795656925562 1102 66-67 0.33436260507077975 1102 68-69 0.008821180416092034 1102 70-71 9.767960884232707E-5 1102 72-73 0.01212030662486896 1102 74-75 -0.07625464118819636 1102 76-77 0.12167364694058591 1102 78-79 -0.014335104247550134 1102 80-81 0.1775951454206286 1102 82-83 0.23092317897795667 1102 84-85 0.16389260311237663 1102 86-87 0.038896565114502124 1102 88-89 0.09765254293748526 1102 90-91 0.10645752751755921 1102 92-93 -0.044566776107281214 1102 94-95 -0.03483253399730302 1102 96-97 -0.03531097590262178 1102 98-99 0.1200169256198329 1102 100-101 0.37084305744737733 1103 1 0.3486007077671225 1103 2 0.39648759457123006 1103 3 0.3043673951307575 1103 4 0.4745191852070363 1103 5 0.43163489101549857 1103 6 0.3594080755976421 1103 7 0.39140091974009295 1103 8 0.3680065353959989 1103 9 0.42783717133589505 1103 10-11 0.4761741678029594 1103 12-13 0.4275933441508428 1103 14-15 0.44880146549454025 1103 16-17 0.6633072431061464 1103 18-19 0.5638315984546196 1103 20-21 0.7359984812573614 1103 22-23 0.5816578929024203 1103 24-25 0.7019384312411105 1103 26-27 0.5567549191125707 1103 28-29 0.16824020976177678 1103 30-31 0.3000271016674745 1103 32-33 0.4069100770927392 1103 34-35 0.2734438300173565 1103 36-37 0.477005999212075 1103 38-39 0.4987751830296361 1103 40-41 0.42054796608952927 1103 42-43 0.5522941976978544 1103 44-45 0.5555891522896488 1103 46-47 0.6586583227245697 1103 48-49 0.4746093094432524 1103 50-51 0.6278239336998794 1103 52-53 0.6006948964618815 1103 54-55 0.5922576012691678 1103 56-57 0.7346659823317268 1103 58-59 0.5943898714891631 1103 60-61 0.6212742380093488 1103 62-63 0.6223001362008418 1103 64-65 0.7995279441509453 1103 66-67 0.6427066191414497 1103 68-69 0.715179217901067 1103 70-71 0.741226143399011 1103 72-73 0.760779615492428 1103 74-75 0.74263106135189 1103 76-77 0.6678584173384223 1103 78-79 0.6734983069968052 1103 80-81 0.5773695688173923 1103 82-83 0.5775567823332359 1103 84-85 0.6237112991381153 1103 86-87 0.7312944613461347 1103 88-89 0.7154972081013575 1103 90-91 0.8388946658505212 1103 92-93 0.8375980959487777 1103 94-95 0.8634542254375681 1103 96-97 0.8615922460834291 1103 98-99 0.963154782005816 1103 100-101 1.3973306217666703 1104 1 0.19523085851488275 1104 2 0.27599343372977003 1104 3 0.21691754970551003 1104 4 0.3435303851496343 1104 5 0.36019235276049244 1104 6 0.32053775694043907 1104 7 0.403748961095566 1104 8 0.34321298388403676 1104 9 0.5057258057561356 1104 10-11 0.5211929440014416 1104 12-13 0.24903782580219058 1104 14-15 0.48950724724814165 1104 16-17 0.33195487844128024 1104 18-19 0.6414438221186103 1104 20-21 0.6170721927855922 1104 22-23 0.4104705375359572 1104 24-25 0.38413467839328774 1104 26-27 0.13588433734940253 1104 28-29 0.3806344441533156 1104 30-31 0.4506908462633348 1104 32-33 0.24183440422913094 1104 34-35 0.20703274488463563 1104 36-37 0.23222850314371613 1104 38-39 0.32109359506554114 1104 40-41 0.3891183140471668 1104 42-43 0.41134638835399784 1104 44-45 0.41848254055631884 1104 46-47 0.4835092791745481 1104 48-49 0.5503232833598801 1104 50-51 0.4771884430969422 1104 52-53 0.6251325074597105 1104 54-55 0.7825193917052644 1104 56-57 0.6180234835426148 1104 58-59 0.6953836944786431 1104 60-61 0.6220871756472519 1104 62-63 0.730707268764391 1104 64-65 0.524344367942895 1104 66-67 0.557537424066318 1104 68-69 0.7793153333806657 1104 70-71 0.8285879380208812 1104 72-73 0.6873654269052487 1104 74-75 0.4577828256567855 1104 76-77 0.6559026430594237 1104 78-79 0.5846830014052031 1104 80-81 0.72979806678131 1104 82-83 0.9146387278822594 1104 84-85 0.8666624511236698 1104 86-87 0.9172305505151392 1104 88-89 0.9761361071224819 1104 90-91 0.9535113981940206 1104 92-93 0.9342376646941837 1104 94-95 0.9699079456961321 1104 96-97 0.9815129555127875 1104 98-99 1.3133606325257468 1104 100-101 1.5085051507172942 1105 1 0.3607761594038017 1105 2 0.3136144382511965 1105 3 0.4296337722394057 1105 4 0.356650364749278 1105 5 0.4525111606473189 1105 6 0.42150350461025 1105 7 0.5161029465210802 1105 8 0.4832879159645742 1105 9 0.6169348865304372 1105 10-11 0.5979648163553648 1105 12-13 0.5169216483844679 1105 14-15 0.3229970160113993 1105 16-17 0.5187675515092067 1105 18-19 0.45057690502448366 1105 20-21 0.5269317114810121 1105 22-23 0.36622137856029013 1105 24-25 0.5388638669233217 1105 26-27 0.5712065420264452 1105 28-29 0.49567398619145564 1105 30-31 0.38954764164186173 1105 32-33 0.4806988824344245 1105 34-35 0.3716127884969396 1105 36-37 0.5174632543672502 1105 38-39 0.47072612709638406 1105 40-41 0.12886190379076368 1105 42-43 0.4105388742225031 1105 44-45 0.31355637742538534 1105 46-47 0.6078380126196947 1105 48-49 0.6394538816068902 1105 50-51 0.7340217453720399 1105 52-53 0.6606325614029629 1105 54-55 0.6512772584294666 1105 56-57 0.740521742646763 1105 58-59 0.6242472572833009 1105 60-61 0.5434508283573294 1105 62-63 0.6286597137014098 1105 64-65 0.7036880256876756 1105 66-67 0.8138280245364413 1105 68-69 0.8005576955066189 1105 70-71 0.8701884833381186 1105 72-73 0.8635803760514769 1105 74-75 0.9260669725524302 1105 76-77 0.501561519012828 1105 78-79 0.5683918124632434 1105 80-81 0.5375351637189674 1105 82-83 0.853396300370548 1105 84-85 0.7751508304395394 1105 86-87 0.7859160025105503 1105 88-89 0.6290335135963225 1105 90-91 0.6491102827129573 1105 92-93 0.8025922647458366 1105 94-95 0.8129525355149134 1105 96-97 0.9245094867982395 1105 98-99 0.9203860054697728 1105 100-101 1.3693292728317417 1106 1 0.1811816275048237 1106 2 0.2732579427893711 1106 3 0.2718034730804568 1106 4 0.16636527733450635 1106 5 0.2981957183144388 1106 6 0.2787774939576195 1106 7 0.2991624154844814 1106 8 0.2752287941607108 1106 9 0.502505048897838 1106 10-11 0.40008191985554475 1106 12-13 0.5657227551646926 1106 14-15 0.5411215391121047 1106 16-17 0.7231731307012907 1106 18-19 0.5632357839234956 1106 20-21 0.3336768967342465 1106 22-23 0.3254824735121602 1106 24-25 0.2453016646571342 1106 26-27 0.2245803808667972 1106 28-29 0.38758274767931766 1106 30-31 0.41791778634864585 1106 32-33 0.25709092988169857 1106 34-35 0.21437043517540388 1106 36-37 0.20379739916375428 1106 38-39 0.312262929390279 1106 40-41 0.15039683320343755 1106 42-43 -0.0024895604582653164 1106 44-45 0.25753274992507613 1106 46-47 0.22581742303241725 1106 48-49 0.3669788619612149 1106 50-51 0.29461105492334383 1106 52-53 0.4321062483120599 1106 54-55 0.5032253753470997 1106 56-57 0.37783055120589637 1106 58-59 0.34828159296414185 1106 60-61 0.49562648610003635 1106 62-63 0.5783279003927717 1106 64-65 0.5120033512325293 1106 66-67 0.5190331388551925 1106 68-69 0.34417855883364723 1106 70-71 0.618763259900895 1106 72-73 0.6890149396826182 1106 74-75 0.41169821719593713 1106 76-77 0.5620056446639303 1106 78-79 0.5156637081715338 1106 80-81 0.5968704261911633 1106 82-83 0.730517489671481 1106 84-85 0.5127137953885317 1106 86-87 0.6800705301652776 1106 88-89 0.8028961744335597 1106 90-91 0.7361905356729821 1106 92-93 0.9006721342280919 1106 94-95 0.7670961427767082 1106 96-97 0.8708331918842518 1106 98-99 1.1876571926165376 1106 100-101 1.4950214110389801 1107 1 0.2930645923163162 1107 2 0.2693196073505355 1107 3 0.23813329608855582 1107 4 0.18533313733408363 1107 5 0.3811137805793692 1107 6 0.31424083505041267 1107 7 0.3777734785796554 1107 8 0.426598938839021 1107 9 0.5128054247053555 1107 10-11 0.3583882172317061 1107 12-13 0.3659444901124971 1107 14-15 0.322172019763002 1107 16-17 0.39580399429608093 1107 18-19 0.5402995142600346 1107 20-21 0.6464766904898411 1107 22-23 0.6603673900945921 1107 24-25 0.6252582208196813 1107 26-27 0.46633458357780455 1107 28-29 0.3792517246633764 1107 30-31 0.586038811692795 1107 32-33 0.43013728046239663 1107 34-35 0.6244289771991873 1107 36-37 0.5535047445665029 1107 38-39 0.5337499730439887 1107 40-41 0.3802619082002252 1107 42-43 0.5913675616179148 1107 44-45 0.48256734916428456 1107 46-47 0.7046886605617928 1107 48-49 0.7772519026115816 1107 50-51 0.6455364527081784 1107 52-53 0.7755792531324417 1107 54-55 0.8108325996756491 1107 56-57 0.8460704113801896 1107 58-59 0.9387751211759863 1107 60-61 0.8208376811886637 1107 62-63 0.9287741463706922 1107 64-65 0.9334971315126452 1107 66-67 0.8519921020661769 1107 68-69 0.8707209594193657 1107 70-71 1.0202197407390798 1107 72-73 0.8325176670909507 1107 74-75 0.8765791103097307 1107 76-77 0.6622434068602772 1107 78-79 0.7955908008471546 1107 80-81 1.0662512561953612 1107 82-83 1.159283217565818 1107 84-85 0.95891109933142 1107 86-87 1.013372802222083 1107 88-89 0.8902283926454722 1107 90-91 0.8118651514354305 1107 92-93 0.7586258321362926 1107 94-95 0.8857056475643397 1107 96-97 1.0413942085078034 1107 98-99 1.2140508877264509 1107 100-101 1.8746780577758422 1108 1 0.01873535552687855 1108 2 0.23549803362859834 1108 3 0.1112399113012863 1108 4 0.19729132759302814 1108 5 0.365352127438328 1108 6 0.17165107821314507 1108 7 -0.002831509268737875 1108 8 0.26111957978742595 1108 9 0.16725470409537024 1108 10-11 0.12584214277023165 1108 12-13 0.2527533265609776 1108 14-15 0.33923845539677444 1108 16-17 0.297257151382901 1108 18-19 0.4365974235864698 1108 20-21 0.2859832213966982 1108 22-23 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-1.5612607446113955 2316 68-69 -2.1394103541473584 2316 70-71 -2.1270885353279567 2316 72-73 -1.770085009545987 2316 74-75 -1.1830151970157345 2316 76-77 -1.1988560833581339 2316 78-79 -2.080248115430237 2316 80-81 -1.668346624365558 2316 82-83 -1.4449068830973317 2316 84-85 -1.2585104085966243 2316 86-87 -1.9861114040772456 2316 88-89 -2.3158669798444684 2316 90-91 -2.3081716245306474 2316 92-93 -2.7863866117979406 2316 94-95 -3.4604470555031988 2316 96-97 -4.524552517387605 2316 98-99 -5.747815543598232 2316 100-101 -6.14197458063315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4682.0 3 1784.0 4 1833.0 5 2047.0 6 2336.0 7 2707.0 8 3244.0 9 4173.0 10 5395.0 11 6723.0 12 8658.0 13 10584.0 14 12205.0 15 15401.0 16 18751.0 17 23802.0 18 19873.0 19 14124.0 20 14418.0 21 15525.0 22 17194.0 23 19437.0 24 22703.0 25 25980.0 26 30335.0 27 35919.0 28 43140.0 29 52581.0 30 63502.0 31 78939.0 32 98615.0 33 127832.0 34 169750.0 35 231526.0 36 325770.0 37 426405.0 38 331931.0 39 46550.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.832882122413956 18.501117715740975 14.075254531340784 22.590745630504284 2 15.522392021344 20.028623633891478 40.02749341562236 24.421490929142166 3 19.541722837527114 24.792337274319433 29.486201982208836 26.179737905944616 4 12.571141771364799 16.344813941517504 37.39412776869428 33.689916518423416 5 13.589577774827516 37.68651954381352 33.85608626943782 14.867816411921147 6 31.78279469512378 35.819904847958 17.633274951717404 14.764025505200816 7 29.054303760011546 32.870285318598704 20.301313089917304 17.77409783147245 8 26.87950253323696 33.622597364337366 19.776247863277142 19.721652239148533 9 26.89202868638356 16.765682192278245 20.52610258790643 35.81618653343177 10-11 25.221545054471168 26.417322000081793 26.967333145050105 21.393799800396938 12-13 26.416810832105785 23.9158259395019 27.50402521325066 22.163338015141655 14-15 23.195137663645582 25.212547376371834 26.29121821958829 25.301096740394293 16-17 22.216871752158802 28.754777417828414 26.772959975676653 22.25539085433613 18-19 22.010345435983172 27.02127413563921 29.355742021021474 21.612638407356147 20-21 22.95136071551563 25.963596250254966 29.265737356467845 21.81930567776156 22-23 22.47833015648094 26.02485329577477 29.24276753253286 22.254049015211432 24-25 22.565173089718844 26.272188513305135 29.235515147182923 21.927123249793098 26-27 22.088054546586953 26.470021058317993 29.3867875160281 22.055136879066954 28-29 21.733746474580812 26.739188964180588 29.517084452114865 22.009980109123735 30-31 21.862880815282438 26.31261739289744 29.43978439943634 22.38471739238378 32-33 21.71106402349178 26.571171205490373 29.365795968979242 22.351968802038602 34-35 22.66356020279051 26.43367188824264 28.888851316066578 22.01391659290028 36-37 22.521450661872507 26.058578659561032 29.05278928162488 22.367181396941582 38-39 22.094970171867317 26.537717129607536 29.176436192537796 22.19087650598735 40-41 22.278575772971497 26.33134461108203 29.324079832524447 22.065999783422026 42-43 22.039912333171614 26.520242832235212 28.758567600553942 22.68127723403923 44-45 22.209809605439293 26.609418822021265 28.61638914837678 22.56438242416266 46-47 22.532434903345873 26.88848351204346 28.11891685057003 22.460164734040642 48-49 22.615371774897707 26.95147579996233 27.880313821500113 22.55283860363985 50-51 22.24481178884329 27.13265423389232 28.001049487486725 22.621484489777664 52-53 22.72159554441927 27.239967709709255 27.555422962574248 22.483013783297224 54-55 22.208791291106515 26.943829434784423 27.84506679022743 23.002312483881635 56-57 22.417272364960922 27.388122220419525 27.526584871890474 22.668020542729074 58-59 22.79299463391081 26.800161119653172 27.665323146781546 22.741521099654474 60-61 22.519138375031673 26.98804097692608 28.267435569040266 22.22538507900198 62-63 22.276273439424482 27.188116569998694 28.135056799078356 22.400553191498467 64-65 22.06942688408508 27.228805514157333 27.900291304604636 22.801476297152952 66-67 22.14124617597922 27.465361766170037 27.421145021078928 22.972247036771808 68-69 22.12631173842009 27.13738955626442 27.91702135447112 22.81927735084437 70-71 22.27415516991198 27.131513513814298 27.690788485253435 22.903542831020285 72-73 22.381560784565504 26.944068141204667 27.93029651268552 22.744074561544306 74-75 22.013861765432903 27.6383528105013 27.604088974685208 22.743696449380586 76-77 22.238530680661977 27.241396432085974 27.700580127394314 22.819492759857734 78-79 22.24288928539402 27.285873851870445 27.653450803864576 22.817786058870954 80-81 22.261441973661935 27.689534526092253 27.34404780224684 22.70497569799897 82-83 22.418869775588508 27.545410496506356 27.38682260928225 22.648897118622884 84-85 22.102360740133655 27.49206062457205 27.256035841006582 23.14954279428772 86-87 22.227510770304136 27.484917293908918 27.44984023781345 22.837731697973492 88-89 22.181644086517892 27.379347997535348 27.178576114074215 23.260431801872546 90-91 22.151124355255728 27.268394459688196 27.526902109301716 23.053579075754364 92-93 22.436038758810522 27.028983490904263 27.67509209177285 22.859885658512365 94-95 21.9664688534632 26.786520336120496 27.870686797140483 23.376324013275823 96-97 22.033877265274317 26.711301004186982 27.961831520286317 23.292990210252384 98-99 21.740454766901355 27.07838337447418 27.546001974418182 23.63515988420628 100-101 21.583722012628705 26.923047279930017 27.834639920906813 23.65859078653446 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 160.0 2 191.0 3 256.5 4 240.0 5 284.0 6 456.5 7 901.0 8 1490.5 9 1808.0 10 1820.5 11 1704.5 12 1782.0 13 2042.5 14 2262.5 15 2482.5 16 2700.5 17 2906.5 18 3124.0 19 3313.5 20 3650.0 21 4233.5 22 4990.5 23 5864.5 24 6803.0 25 8404.0 26 11054.0 27 14010.0 28 16520.0 29 19762.5 30 24263.5 31 29473.5 32 36261.0 33 42626.0 34 48765.0 35 54362.5 36 61246.5 37 68121.0 38 70388.0 39 70319.0 40 69636.5 41 72340.5 42 75858.0 43 78690.0 44 81008.5 45 82848.0 46 82556.0 47 85331.5 48 98979.0 49 100156.0 50 88754.5 51 82149.0 52 77544.5 53 76427.0 54 75383.5 55 70182.0 56 63661.5 57 56863.5 58 51640.5 59 46519.5 60 39677.5 61 33195.5 62 27703.5 63 21599.5 64 16095.5 65 12679.5 66 9253.0 67 7138.0 68 5237.5 69 3343.0 70 2679.0 71 2248.0 72 1846.0 73 1603.5 74 1259.0 75 1013.5 76 785.5 77 478.0 78 277.5 79 180.5 80 136.0 81 94.5 82 62.0 83 37.0 84 21.5 85 18.0 86 11.5 87 6.0 88 6.5 89 6.5 90 4.5 91 4.0 92 3.5 93 2.0 94 3.5 95 3.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035054139170496657 2 0.023711835287491025 3 0.021700181391259836 4 0.053929444877687166 5 0.058894377898172645 6 0.05033414855250802 7 0.051660984101086035 8 0.044256385717086136 9 0.04545481782547918 10-11 0.05356563513049642 12-13 0.0473380682815254 14-15 0.041324507166196 16-17 0.05054815428614963 18-19 0.053501433410403934 20-21 0.012005721657294641 22-23 0.020480348709502626 24-25 0.005414345061132878 26-27 0.01089289184235824 28-29 0.005949359395236917 30-31 0.009159445399861153 32-33 0.0034026911649016897 34-35 0.0077898087045548126 36-37 0.009031041959676182 38-39 0.010807289548901594 40-41 0.0019260516027745415 42-43 0.0017334464424970872 44-45 0.0022898613499652883 46-47 5.992160541965241E-4 48-49 0.0011128298149364016 50-51 3.2100860046242356E-4 52-53 0.003424091738265852 54-55 0.006569976022797602 56-57 0.016136032316577825 58-59 0.010015468334427615 60-61 0.010357877508254202 62-63 0.005328742767676232 64-65 0.0 66-67 0.0071477915036299655 68-69 0.017612671878704973 70-71 0.006291768569063503 72-73 0.002610869950427712 74-75 0.004429918686381446 76-77 0.007490200677456551 78-79 0.006013561115329402 80-81 0.011192499869456503 82-83 0.006077762835421887 84-85 0.017227461558150067 86-87 0.004322915819560637 88-89 0.006527174876069279 90-91 0.0039163049256415675 92-93 0.017163259838057582 94-95 0.011106897575999856 96-97 0.009309249413410284 98-99 0.02174298253798816 100-101 0.03907744696295903 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2336386.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.3197158796487 #Duplication Level Percentage of deduplicated Percentage of total 1 75.43332706260291 36.44916931521588 2 13.394795161842065 12.944653929726032 3 4.620334630067949 6.697597697813553 4 2.15583072742403 4.1667651293498205 5 1.1138566636872456 2.691061876001056 6 0.6825046389096058 1.9787058143192628 7 0.446952378526198 1.5117628359483362 8 0.3052995946959345 1.1801591739103565 9 0.2361007873985118 1.0267490669451689 >10 1.3444215153403627 12.337554999160647 >50 0.1536729546021925 5.222971762292153 >100 0.10453796622431441 9.43670213707205 >500 0.006318938576456793 2.072067952868737 >1k 0.0017799826975934269 1.4051599186431922 >5k 2.66997404639014E-4 0.8789183907338811 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8309 0.355634728165637 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6666 0.2853124440910021 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5463 0.23382266457682935 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3798 0.1625587552741713 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3628 0.15528256033035637 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2659 0.11380824915061125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.1400573364161574E-5 0.0 18-19 0.0 0.0 0.0 6.420172009248472E-5 0.0 20-21 0.0 0.0 0.0 1.0700286682080787E-4 0.0 22-23 0.0 0.0 0.0 3.424091738265852E-4 0.0 24-25 0.0 0.0 0.0 5.564149074682009E-4 0.0 26-27 0.0 0.0 0.0 7.704206411098167E-4 0.0 28-29 0.0 0.0 0.0 0.0013268355485780174 0.0 30-31 0.0 0.0 0.0 0.0028248756840693274 0.0 32-33 0.0 0.0 0.0 0.005050535313942131 0.0 34-35 0.0 0.0 0.0 0.006826782903167542 0.0 36-37 0.0 0.0 0.0 0.01020807349470507 0.0 38-39 0.0 0.0 0.0 0.015066003648369748 0.0 40-41 0.0 0.0 0.0 0.020972561896878343 0.0 42-43 0.0 0.0 0.0 0.02685771957202277 0.0 44-45 0.0 0.0 0.0 0.029703995829456264 0.0 46-47 0.0 0.0 0.0 0.03434792024947932 0.0 48-49 0.0 0.0 0.0 0.04113190200591854 0.0 50-51 0.0 0.0 0.0 0.04866490383010341 0.0 52-53 0.0 0.0 0.0 0.0617620547289703 0.0 54-55 0.0 0.0 0.0 0.07280475058487768 0.0 56-57 4.280114672832315E-5 0.0 0.0 0.09932006098307386 0.0 58-59 4.280114672832315E-5 0.0 0.0 0.14841297628046052 0.0 60-61 4.280114672832315E-5 0.0 0.0 0.188346446177986 0.0 62-63 4.280114672832315E-5 0.0 0.0 0.21115945738418224 0.0 64-65 4.280114672832315E-5 0.0 0.0 0.25308318060457474 0.0 66-67 4.280114672832315E-5 0.0 0.0 0.34159595203874704 0.0 68-69 4.280114672832315E-5 0.0 0.0 0.4625947938397166 0.0 70-71 4.280114672832315E-5 0.0 0.0 0.5689342428862354 0.0 72-73 4.280114672832315E-5 0.0 0.0 0.6882210388180721 0.0 74-75 4.280114672832315E-5 0.0 0.0 0.830213843089284 0.0 76-77 4.280114672832315E-5 0.0 0.0 0.9249327807990632 0.0 78-79 4.280114672832315E-5 0.0 0.0 1.0504471435798708 0.0 80-81 4.280114672832315E-5 0.0 0.0 1.175490693746667 0.0 82-83 4.280114672832315E-5 0.0 0.0 1.2722426859260412 0.0 84-85 4.280114672832315E-5 0.0 0.0 1.4349298446403975 0.0 86-87 4.280114672832315E-5 0.0 0.0 1.7060109074442322 0.0 88-89 4.280114672832315E-5 0.0 0.0 2.0750851956825627 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13170 0.0 26.996264 1 TACATGG 13590 0.0 25.532375 2 ACATGGG 13425 0.0 24.748604 3 GTATCAA 17540 0.0 24.118387 1 GAGTACT 8375 0.0 22.329208 12-13 GGTATCA 12990 0.0 22.17437 1 GTACTTT 8870 0.0 21.136227 14-15 ATCAACG 19980 0.0 20.9351 3 TCAACGC 20005 0.0 20.885624 4 CAACGCA 20135 0.0 20.702677 5 TATCAAC 20405 0.0 20.545647 2 CATGGGG 5620 0.0 20.468037 4 AACGCAG 20475 0.0 20.404886 6 CATGGGA 7160 0.0 19.6506 4 GGACCGA 270 2.2850145E-8 19.364136 6 AGTACTT 8850 0.0 19.278118 12-13 ATGGGTA 1135 0.0 19.263735 5 GTATAGG 935 0.0 18.80953 1 AGAGTAC 16120 0.0 18.43964 10-11 ACGCAGA 22640 0.0 18.36965 7 >>END_MODULE