FastQCFastQC Report
Thu 26 May 2016
SRR936673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336386
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106090.4540773656407803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91200.3903464581623071No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67690.2897209622040194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53580.22932854417035542No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30660.13122831586903877No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.11830236955708516No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25140.10760208287500439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG9500.024.9992545
GTACATG128650.024.8593941
TACATGG133650.023.5632152
GTATCAA209400.023.5335451
GGTATCA158050.022.881021
ACATGGG130500.022.8575573
CGAATCG853.1529362E-622.35251482-83
GAGTACT117650.022.28673412-13
TACCTGG16750.021.552062
GTACTTT123300.021.43884514-15
GTAAGGT8850.020.9302374
AGTACTT121400.020.4049212-13
GTATAGG9650.020.1902521
TCAACGC244050.020.103624
ATCAACG244200.020.0925643
CATGGGA72250.020.050024
CAACGCA246600.019.9162775
TATCAAC248050.019.8194272
AACGCAG251200.019.551156
CGTATAG2451.749795E-719.396311
Produced by FastQC (version 0.11.5)