##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936673_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2336386 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94597853265685 31.0 31.0 34.0 30.0 34.0 2 32.09761614733182 33.0 31.0 34.0 30.0 34.0 3 32.20011633351681 33.0 31.0 34.0 30.0 34.0 4 35.935277817963296 37.0 35.0 37.0 35.0 37.0 5 35.72842030383678 37.0 35.0 37.0 33.0 37.0 6 35.8330733877022 37.0 35.0 37.0 35.0 37.0 7 35.77838721855036 37.0 35.0 37.0 33.0 37.0 8 35.752476688355436 37.0 35.0 37.0 33.0 37.0 9 37.40101978012195 39.0 37.0 39.0 34.0 39.0 10-11 37.27409554756791 39.0 37.0 39.0 34.0 39.0 12-13 37.18276453462741 39.0 37.0 39.0 33.0 39.0 14-15 38.29905781835707 40.0 38.0 41.0 33.0 41.0 16-17 38.234789328475685 40.0 38.0 41.0 33.0 41.0 18-19 38.38942152538151 40.0 38.0 41.0 33.5 41.0 20-21 38.428089151364546 40.0 38.0 41.0 34.0 41.0 22-23 38.339585368171186 40.0 38.0 41.0 34.0 41.0 24-25 38.21860664290918 40.0 38.0 41.0 33.5 41.0 26-27 38.015283433473755 40.0 37.0 41.0 33.0 41.0 28-29 37.937829622331236 40.0 37.0 41.0 33.0 41.0 30-31 37.89284369106817 40.0 37.0 41.0 33.0 41.0 32-33 37.73899282909588 40.0 37.0 41.0 33.0 41.0 34-35 37.571309064512455 40.0 36.5 41.0 33.0 41.0 36-37 37.3066222790241 39.5 36.0 41.0 32.0 41.0 38-39 37.14796977040609 39.0 36.0 41.0 31.0 41.0 40-41 36.9331212821854 39.0 36.0 41.0 31.0 41.0 42-43 36.67210897514366 39.0 35.0 41.0 31.0 41.0 44-45 36.3886740033539 39.0 35.0 40.5 30.0 41.0 46-47 35.99376130485288 39.0 35.0 40.0 29.5 41.0 48-49 35.59982811059474 39.0 35.0 40.0 28.0 41.0 50-51 35.26525646875131 38.5 35.0 40.0 27.0 41.0 52-53 34.989611091660365 38.0 34.0 40.0 26.0 41.0 54-55 34.72072016353462 38.0 34.0 40.0 26.0 41.0 56-57 34.411109508445946 38.0 34.0 40.0 25.0 41.0 58-59 34.08344853975328 37.5 33.0 40.0 24.0 41.0 60-61 33.72717158038098 37.0 33.0 40.0 23.0 41.0 62-63 33.3394085994352 36.5 32.5 39.0 22.5 40.5 64-65 32.931380345542216 36.0 32.0 39.0 22.0 40.0 66-67 32.475570175476136 35.5 31.5 39.0 20.5 40.0 68-69 32.19776526652702 35.0 31.0 38.0 20.0 40.0 70-71 31.770878399374077 35.0 31.0 37.5 18.5 40.0 72-73 31.330955586962084 35.0 31.0 37.0 18.0 39.0 74-75 30.810273216840024 34.0 30.0 36.5 14.5 39.0 76-77 29.392860811526862 32.5 28.5 35.0 12.5 37.0 78-79 30.162824764401087 34.0 30.0 35.0 12.0 37.5 80-81 29.96167071708185 34.0 30.0 35.0 7.5 37.0 82-83 29.63422974628336 34.0 29.5 35.0 7.0 36.5 84-85 29.230095540719727 34.0 29.0 35.0 2.0 36.0 86-87 28.869019930781988 34.0 29.0 35.0 2.0 36.0 88-89 28.483610798900525 33.5 29.0 35.0 2.0 35.0 90-91 28.021507148219516 33.0 27.5 35.0 2.0 35.0 92-93 27.54999409344175 33.0 27.0 35.0 2.0 35.0 94-95 26.97876570909088 33.0 25.5 34.5 2.0 35.0 96-97 26.24575862036496 32.0 25.0 34.0 2.0 35.0 98-99 25.344451216536996 32.0 22.0 34.0 2.0 35.0 100-101 22.17536764044982 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23462444151446604 1101 2 0.374780613725342 1101 3 0.27701069135434153 1101 4 0.16230633188546761 1101 5 0.09303863735122064 1101 6 0.22510856388344536 1101 7 0.12594337684255663 1101 8 0.16781508513039967 1101 9 0.23564004204813926 1101 10-11 0.29542729123708966 1101 12-13 0.23117014008587233 1101 14-15 0.34949019508955814 1101 16-17 0.373507517210129 1101 18-19 0.2728233981110222 1101 20-21 0.14892642945527257 1101 22-23 0.21608722539694014 1101 24-25 0.13607781612081027 1101 26-27 0.21908763468470482 1101 28-29 0.22484556595094318 1101 30-31 0.21042389811353956 1101 32-33 0.2772793918319607 1101 34-35 0.20241884525540144 1101 36-37 0.25311108121069026 1101 38-39 0.30222422081164524 1101 40-41 0.3510664845464859 1101 42-43 0.40552690278112635 1101 44-45 0.4659726081934963 1101 46-47 0.48000741925034873 1101 48-49 0.37470771740836994 1101 50-51 0.5348431152814754 1101 52-53 0.38474087964397796 1101 54-55 0.4160636547152663 1101 56-57 0.3697503407114979 1101 58-59 0.37002751122335553 1101 60-61 0.2955944881336805 1101 62-63 0.4696504709793743 1101 64-65 0.472986195379157 1101 66-67 0.318399997617 1101 68-69 0.32158453885403304 1101 70-71 0.3354166963498564 1101 72-73 0.30776908177521634 1101 74-75 0.37755483827051606 1101 76-77 0.026444577147390902 1101 78-79 -0.0841386933876116 1101 80-81 0.23195912760591852 1101 82-83 0.34195302394319427 1101 84-85 0.31886546268079385 1101 86-87 0.325747134053735 1101 88-89 0.17666119464733399 1101 90-91 0.23307582943780503 1101 92-93 0.34552489081314164 1101 94-95 0.4010283649864519 1101 96-97 0.5126212627686506 1101 98-99 0.599223621399009 1101 100-101 0.9963195213474272 1102 1 0.186390851324024 1102 2 0.2521856623232779 1102 3 0.2225369750719608 1102 4 0.12596723979158497 1102 5 -0.03265757301918626 1102 6 0.08593506106269189 1102 7 0.17014653213850295 1102 8 0.18343858734319696 1102 9 0.16941674631259218 1102 10-11 0.20254766023813175 1102 12-13 0.19671385928359086 1102 14-15 0.30305288915683093 1102 16-17 0.2430773449506347 1102 18-19 0.1464401319869495 1102 20-21 0.09351342828772147 1102 22-23 0.09381052947414759 1102 24-25 0.23680044409952927 1102 26-27 -0.017640857338555804 1102 28-29 0.012442905329479004 1102 30-31 0.17592421241025846 1102 32-33 0.18749423336731041 1102 34-35 0.1053946824398011 1102 36-37 0.3403005700423378 1102 38-39 0.43230524517861824 1102 40-41 0.3107240326022094 1102 42-43 0.2385123371143223 1102 44-45 0.3074215740682007 1102 46-47 0.4434375177073733 1102 48-49 0.3868554343247226 1102 50-51 0.3063481936548911 1102 52-53 0.2032637868278968 1102 54-55 0.4123585445021618 1102 56-57 0.3755577609419092 1102 58-59 0.2668209776971011 1102 60-61 0.47234065881757914 1102 62-63 0.24215796221285046 1102 64-65 0.33279345499730084 1102 66-67 0.40154321654141967 1102 68-69 0.3345885482227047 1102 70-71 0.29966477218373555 1102 72-73 0.3770892535737964 1102 74-75 0.31636636966611675 1102 76-77 0.13731511545582364 1102 78-79 0.1510270101238298 1102 80-81 0.5008428751426557 1102 82-83 0.4736292844596903 1102 84-85 0.42942382502377896 1102 86-87 0.3979893605470082 1102 88-89 0.30453323473706106 1102 90-91 0.5301164210791249 1102 92-93 0.46065221986781424 1102 94-95 0.3333951168888447 1102 96-97 0.6480101975445187 1102 98-99 0.6280690057897047 1102 100-101 0.958770959463493 1103 1 0.08335215070903601 1103 2 0.09949875701164501 1103 3 0.15906364483988256 1103 4 0.08273345605473281 1103 5 -0.09701786470438378 1103 6 0.13803504301314717 1103 7 0.06354247708340921 1103 8 0.14672655387695244 1103 9 0.07828789818670145 1103 10-11 0.01092946732209299 1103 12-13 0.0919941880699966 1103 14-15 0.13550300949661676 1103 16-17 0.24079545832341154 1103 18-19 0.06670342188939316 1103 20-21 0.1269761878637965 1103 22-23 0.17482359005628467 1103 24-25 0.16967542623503817 1103 26-27 0.23275806891282969 1103 28-29 0.2486073173214436 1103 30-31 0.10895022425236078 1103 32-33 0.16467992218519356 1103 34-35 0.21597575820404558 1103 36-37 0.2858587735603493 1103 38-39 0.3310256634269919 1103 40-41 0.3089773690633706 1103 42-43 0.1908416820866634 1103 44-45 0.2383733829229513 1103 46-47 0.3258476528967478 1103 48-49 0.3312868935990778 1103 50-51 0.41986595025023377 1103 52-53 0.297764825103755 1103 54-55 0.22179734601240142 1103 56-57 0.12642550560860855 1103 58-59 0.32982662828638354 1103 60-61 0.18587908413432075 1103 62-63 0.18659020439332608 1103 64-65 0.13608316954688604 1103 66-67 0.22841570021000024 1103 68-69 0.20611023870775114 1103 70-71 0.35237043877277685 1103 72-73 -0.0023817097195824033 1103 74-75 0.11822051909698317 1103 76-77 -0.046929525338310185 1103 78-79 0.010871513581999892 1103 80-81 0.1677862160848882 1103 82-83 0.3561397739781462 1103 84-85 0.13067062436124033 1103 86-87 0.02465460185005952 1103 88-89 0.013923538772246502 1103 90-91 -0.056843736686420954 1103 92-93 0.029834999691271236 1103 94-95 0.06667679739824095 1103 96-97 0.13645720789653026 1103 98-99 0.5187439961243747 1103 100-101 0.7760625228102782 1104 1 0.12800960468043954 1104 2 0.2498010298884097 1104 3 0.16900755543169055 1104 4 0.14641765713944466 1104 5 0.023182693797828335 1104 6 0.06609768615175682 1104 7 0.15022064466994323 1104 8 0.11212632545313994 1104 9 0.10258872398065222 1104 10-11 0.10363948866955042 1104 12-13 0.05704803509780021 1104 14-15 0.20058286813695503 1104 16-17 0.280346115191378 1104 18-19 0.082495759526104 1104 20-21 0.04855730522699986 1104 22-23 0.09589972335533048 1104 24-25 0.2288717272043428 1104 26-27 0.26166758437927484 1104 28-29 0.22684471663855987 1104 30-31 0.00936715751313244 1104 32-33 0.18931022840465772 1104 34-35 0.0942605464934374 1104 36-37 0.2073756093523258 1104 38-39 0.05898605401976198 1104 40-41 0.11552766117078761 1104 42-43 0.22999199930874425 1104 44-45 0.28758107518464726 1104 46-47 0.3318301742890597 1104 48-49 0.3237587741489705 1104 50-51 0.1481511870157064 1104 52-53 0.1999288716735137 1104 54-55 0.29582878717533134 1104 56-57 0.2411971116911431 1104 58-59 -0.003807134188718919 1104 60-61 0.145604287891949 1104 62-63 0.2546241749613358 1104 64-65 0.23919499556208024 1104 66-67 -0.06311602498777091 1104 68-69 0.1681709563890692 1104 70-71 -0.005141236554461415 1104 72-73 -0.015136219894007041 1104 74-75 0.0018027068230921373 1104 76-77 0.1635954607589305 1104 78-79 0.2472254329639405 1104 80-81 0.29496177354065267 1104 82-83 0.20614470721500666 1104 84-85 0.12803313654483972 1104 86-87 0.17535841030891675 1104 88-89 0.37605197634138676 1104 90-91 0.495586854165861 1104 92-93 0.39433258688996986 1104 94-95 0.44133640405481955 1104 96-97 0.6001429039015029 1104 98-99 0.7269128383983272 1104 100-101 1.0672142392777175 1105 1 0.11558206027533302 1105 2 0.18972786322337498 1105 3 0.14196138981610318 1105 4 0.030302682819701943 1105 5 0.005539981204201183 1105 6 0.09754071717403434 1105 7 0.12100203719047187 1105 8 0.1360401862533962 1105 9 0.021653903775145977 1105 10-11 0.08830505685545376 1105 12-13 0.029409801428705862 1105 14-15 0.14914470835377358 1105 16-17 0.2518429913867095 1105 18-19 0.19678679808082222 1105 20-21 0.18079640787284035 1105 22-23 0.1658010235511611 1105 24-25 0.19834295256617196 1105 26-27 0.2033328615985539 1105 28-29 0.2037666493108432 1105 30-31 0.08234927123859137 1105 32-33 0.04738389854004055 1105 34-35 0.1916214137701573 1105 36-37 0.1108152272379499 1105 38-39 0.18175844292117915 1105 40-41 0.25509176700418124 1105 42-43 0.16393227595589366 1105 44-45 0.10448345751480304 1105 46-47 0.05006037793300777 1105 48-49 0.012669839708003394 1105 50-51 0.007251250112958019 1105 52-53 -0.08151644785035472 1105 54-55 -0.019838131226514122 1105 56-57 -0.020531132014660614 1105 58-59 0.042184555281224334 1105 60-61 -0.12144799129216466 1105 62-63 -0.1309461686735247 1105 64-65 -0.2282193572660134 1105 66-67 0.07207361006160085 1105 68-69 0.04843344752112699 1105 70-71 -0.07969980094472007 1105 72-73 -0.10275019544627284 1105 74-75 -0.11417168811343359 1105 76-77 -0.16910902503110847 1105 78-79 -0.0761315046286235 1105 80-81 -0.007041319205779217 1105 82-83 0.019725819884474305 1105 84-85 0.054338644968161987 1105 86-87 0.00285328570000587 1105 88-89 0.20722087756638885 1105 90-91 0.28554000526908396 1105 92-93 0.2917174834333558 1105 94-95 0.18959943135010704 1105 96-97 0.2080083203924481 1105 98-99 0.4865431800062261 1105 100-101 0.6587650914176315 1106 1 0.05174850030913092 1106 2 0.14429482709422103 1106 3 0.16528196548139107 1106 4 0.07677417105171003 1106 5 -0.06068221443212707 1106 6 -0.04581480944562344 1106 7 0.08916323458111464 1106 8 -0.015185536167805935 1106 9 0.1312194131365274 1106 10-11 0.07215394881121284 1106 12-13 0.140419850540205 1106 14-15 0.18128963446575597 1106 16-17 0.1056679952054651 1106 18-19 0.08962799448872971 1106 20-21 0.09087523267191955 1106 22-23 0.07468107975183358 1106 24-25 0.04022655833962574 1106 26-27 0.07902758124853193 1106 28-29 0.13199479686397808 1106 30-31 0.054447969942209795 1106 32-33 -5.98979907479702E-4 1106 34-35 0.09859663227593529 1106 36-37 0.07748283746644091 1106 38-39 0.13803743741896568 1106 40-41 0.28146903791715516 1106 42-43 0.07768698619873504 1106 44-45 0.09244156123325098 1106 46-47 -0.021615605071481525 1106 48-49 -0.23835917677655516 1106 50-51 -0.1028349985593735 1106 52-53 -0.14428120150772372 1106 54-55 -0.1750063398156314 1106 56-57 -0.11971354459188177 1106 58-59 -0.11679973779691011 1106 60-61 -0.09236581346601014 1106 62-63 -0.012591024819514018 1106 64-65 -0.09626268064031507 1106 66-67 -0.02718677988935525 1106 68-69 0.010134717982616337 1106 70-71 0.02563792831854883 1106 72-73 -0.11674464160574516 1106 74-75 -0.0955283306284791 1106 76-77 -0.26123136975020955 1106 78-79 -0.1279477365269237 1106 80-81 -0.018081704720216862 1106 82-83 0.12424629998297476 1106 84-85 -0.10378113183852022 1106 86-87 -0.01938976016030125 1106 88-89 -0.019956320140433803 1106 90-91 0.15961847471922042 1106 92-93 0.14357338053455138 1106 94-95 0.3751719615236091 1106 96-97 0.4053399453447817 1106 98-99 0.7162017672912135 1106 100-101 0.7820402870998961 1107 1 0.15322855933974822 1107 2 0.1663637561075717 1107 3 0.12528935356178295 1107 4 0.0707502575085357 1107 5 0.07863251520151948 1107 6 0.11183057676109343 1107 7 0.11385870760518202 1107 8 0.05052470228285699 1107 9 0.19032794277897835 1107 10-11 0.06937601577846664 1107 12-13 0.07146131817719237 1107 14-15 0.1944602943109146 1107 16-17 0.14222659330610554 1107 18-19 0.15460210844654654 1107 20-21 0.18223961790479137 1107 22-23 0.15136109999132685 1107 24-25 0.18223667882529782 1107 26-27 0.2958430335889304 1107 28-29 0.19084727918125566 1107 30-31 0.17515619295711105 1107 32-33 0.19624931533738987 1107 34-35 0.0025464977099645125 1107 36-37 0.1348471545927552 1107 38-39 0.0886897982548831 1107 40-41 0.13096290259017707 1107 42-43 0.02430699494154709 1107 44-45 0.24295000996215776 1107 46-47 0.2634680604972104 1107 48-49 0.12684860928299457 1107 50-51 0.13516140659910292 1107 52-53 0.07454848362161925 1107 54-55 0.1473839855005039 1107 56-57 -0.07495421427176296 1107 58-59 0.05606885097979841 1107 60-61 0.1974429292685187 1107 62-63 0.2195737567638858 1107 64-65 0.17136544203587079 1107 66-67 0.24116654802259774 1107 68-69 0.3480123438326146 1107 70-71 0.11855497638807933 1107 72-73 0.16092660658378577 1107 74-75 0.17440542094324485 1107 76-77 0.13993510302253398 1107 78-79 0.20972649502573049 1107 80-81 0.3958029264809504 1107 82-83 0.309213980832709 1107 84-85 0.27309582368012997 1107 86-87 0.46963296262862997 1107 88-89 0.4098099078188575 1107 90-91 0.2632204785324497 1107 92-93 0.30693101877697515 1107 94-95 0.5054378657917091 1107 96-97 0.7003033177147664 1107 98-99 0.7993332177498083 1107 100-101 0.9037955558265125 1108 1 0.061182034438740374 1108 2 0.08789830938906817 1108 3 0.034321831837168304 1108 4 0.046869501814626346 1108 5 -0.029999510209613334 1108 6 0.12767802790752114 1108 7 0.056777108106537355 1108 8 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-0.9919089785309332 2316 96-97 -1.0805520808200946 2316 98-99 -1.1782901609655063 2316 100-101 -1.6516939547423242 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 10.0 4 27.0 5 65.0 6 162.0 7 342.0 8 863.0 9 1664.0 10 2679.0 11 4397.0 12 6272.0 13 9045.0 14 11921.0 15 16299.0 16 21325.0 17 27886.0 18 23895.0 19 15153.0 20 13307.0 21 13409.0 22 14430.0 23 15811.0 24 18801.0 25 22580.0 26 27421.0 27 34897.0 28 43204.0 29 53426.0 30 66241.0 31 83384.0 32 104314.0 33 134290.0 34 178461.0 35 244220.0 36 350892.0 37 469353.0 38 289640.0 39 16294.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77384649440743 19.349769707785576 14.681326698953676 22.19505709885331 2 15.815545795303807 20.87433059973042 39.300104056401906 24.010019548563864 3 19.851079876350756 25.01573009468875 29.772382319630385 25.360807709330107 4 13.074433019784118 17.04884802015435 36.83857301650553 33.03814594355601 5 13.542441552393761 37.99100825812394 33.60873253591261 14.857817653569688 6 31.873152320879722 36.48520765270331 16.937934246057036 14.703705780359932 7 29.03265984302251 33.263638799410714 20.0460026725036 17.65769868506317 8 27.140292742723165 33.9050995854281 19.254224259176354 19.70038341267239 9 26.95725085828285 16.858853828042104 20.3345302139961 35.84936509967894 10-11 25.42629616955672 26.696512053183252 26.54450303236917 21.332688744890856 12-13 26.742622296868124 24.139924258377548 27.32358913251531 21.793864312239016 14-15 23.206845779491765 25.407262672653264 26.413926970933044 24.971964576921923 16-17 22.12049252004549 28.935895092733798 26.858474749294935 22.085137637925772 18-19 21.87027314835819 26.89247410316617 29.90935573145876 21.32789701701688 20-21 22.800085259884284 25.734489078431388 30.15225223914199 21.313173422542338 22-23 22.406924854194898 25.675608580607772 30.079359236548818 21.83810732864851 24-25 22.567095709401446 26.00926054330575 30.093470024362638 21.33017372293016 26-27 22.07494780132057 26.26272008410001 30.18930635467023 21.473025759909188 28-29 21.662125165651354 26.463767297083507 30.2905918590277 21.583515678237436 30-31 21.901345107221438 26.00979345403837 30.15620689736348 21.93265454137671 32-33 21.791269068296714 26.293827843731187 30.158859582048485 21.756043505923607 34-35 22.6407926491525 26.10938178194529 29.664308957497028 21.585516611405183 36-37 22.49465454101824 25.89543661537737 29.714887451066847 21.895021392537547 38-39 22.00188428955392 26.35907915514312 29.905669947978712 21.733366607324246 40-41 22.192940874338575 26.27544179725746 29.874472331159346 21.657144997244618 42-43 22.092909782423533 26.400482114592954 29.294410911951594 22.212197191031915 44-45 22.255555232164962 26.390477307396647 29.14647853323723 22.20748892720116 46-47 22.73796719116686 26.818141546142048 28.473392418965553 21.970498843725537 48-49 22.91664169918147 26.968158324102497 28.1108059846634 22.004393992052634 50-51 22.508828901013157 27.09333018313523 28.218520168758165 22.17932074709345 52-53 22.98100330540132 27.279501696572932 27.713173773796697 22.02632122422905 54-55 22.455704817201447 27.116591726714933 27.914086927490512 22.513616528593104 56-57 22.6618095343265 27.405817087963403 27.69560572480759 22.236767652902508 58-59 23.10571859949259 26.86769097755383 27.69121296200191 22.33537746095167 60-61 22.715074905946228 27.120680102033283 28.333283040059342 21.830961951961147 62-63 22.521598009817794 27.176371209045534 28.270808770481647 22.031222010655025 64-65 22.338837419395436 27.234278618433095 27.998177361398884 22.42870660077259 66-67 22.41214373398119 27.51778868954371 27.535252080626076 22.53481549584902 68-69 22.41945853122357 27.133126911131733 27.964800934121815 22.482613623522884 70-71 22.587416029464322 27.312193837916265 27.726166706257743 22.374223426361667 72-73 22.74778924787239 27.04604651969052 27.909289021773347 22.296875210663742 74-75 22.443450496190582 27.825003279829485 27.383804683532304 22.347741540447625 76-77 22.596191401129598 27.298375628151728 27.69735804429062 22.408074926428053 78-79 22.593504606853053 27.360161485972277 27.518788238849258 22.527545668325413 80-81 22.655863433943594 27.809642353899967 27.2058609513602 22.32863326079624 82-83 22.760824454561174 27.656611952998865 27.17664033995867 22.40592325248129 84-85 22.449496289802866 27.662177733338357 27.03664911157301 22.85167686528577 86-87 22.416481919851662 27.634378295133068 27.29998281474747 22.6491569702678 88-89 22.48891352549889 27.662294857416807 26.886027617734936 22.962763999349363 90-91 22.49543893654236 27.515990476345397 27.181904885025244 22.806665702087003 92-93 22.729210475967502 27.225013129414727 27.418968933258057 22.626807461359714 94-95 22.361597508579667 27.073051929986157 27.477359868306728 23.08799069312745 96-97 22.419463568406478 27.0109978535032 27.621839909431962 22.947698668658365 98-99 22.10036835802037 27.37245507239181 27.07247399108168 23.454702578506144 100-101 21.796341366324786 27.644158539080472 27.22988920001952 23.329610894575225 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 477.0 1 353.0 2 274.0 3 340.0 4 325.0 5 314.0 6 502.0 7 1003.0 8 1607.5 9 1994.5 10 2027.5 11 1946.5 12 2110.5 13 2412.5 14 2757.0 15 3098.5 16 3405.0 17 3731.5 18 4078.5 19 4407.0 20 4754.0 21 5303.0 22 6030.5 23 6888.0 24 7907.5 25 9400.5 26 11881.5 27 14816.5 28 17559.5 29 21093.0 30 25435.5 31 29959.0 32 36128.5 33 42550.5 34 48644.5 35 54128.5 36 60966.0 37 67685.5 38 70128.5 39 69917.5 40 69136.5 41 71653.0 42 75138.5 43 77740.0 44 79851.5 45 81334.5 46 80125.0 47 83782.0 48 98829.0 49 99524.0 50 87775.5 51 80994.0 52 76135.5 53 75194.5 54 73905.5 55 68702.5 56 62354.0 57 56178.5 58 51297.0 59 46623.0 60 40507.5 61 33884.5 62 27887.5 63 21851.0 64 16248.5 65 12670.0 66 9424.0 67 7325.0 68 5373.5 69 3402.5 70 2685.0 71 2234.5 72 1948.0 73 1687.5 74 1379.0 75 1137.0 76 797.5 77 490.5 78 326.0 79 239.0 80 178.5 81 121.5 82 83.0 83 54.0 84 27.5 85 15.0 86 10.0 87 6.5 88 3.0 89 2.5 90 2.5 91 2.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03745100338728276 2 0.006548575449433441 3 0.004280114672832315 4 7.276194943814934E-4 5 8.56022934566463E-4 6 3.852103205549083E-4 7 0.0 8 0.0 9 0.0014980401354913102 10-11 4.922131873757162E-4 12-13 9.416252280231092E-4 14-15 0.0023968642167860963 16-17 0.003210086004624236 18-19 0.0 20-21 0.0 22-23 0.002054455042959511 24-25 9.416252280231092E-4 26-27 0.005136137607398777 28-29 0.006591376596161765 30-31 0.0012626338284855328 32-33 6.206166275606857E-4 34-35 0.009416252280231092 36-37 0.012455133697942034 38-39 0.009715860307329354 40-41 0.0020758556163236724 42-43 5.136137607398778E-4 44-45 0.0012840344018496942 46-47 1.2840344018496944E-4 48-49 5.992160541965241E-4 50-51 0.0010700286682080787 52-53 0.002503867083606904 54-55 0.0030388814177109433 56-57 0.0011984321083930482 58-59 7.918212144739781E-4 60-61 0.0038307026321849212 62-63 0.00502913474057797 64-65 0.00909524367976867 66-67 0.0029960802709826204 68-69 0.0031672848578959125 70-71 4.280114672832315E-5 72-73 8.774235079306245E-4 74-75 0.005842356528416109 76-77 0.0028890774041618124 78-79 0.0035952963251791445 80-81 0.004708126140115546 82-83 0.004365716966288961 84-85 0.005221739900855424 86-87 0.0034454923116300135 88-89 0.008817036226034568 90-91 0.01318275319232353 92-93 0.0012412332551213713 94-95 0.002054455042959511 96-97 8.988240812947861E-4 98-99 0.0030816825644392662 100-101 0.0044513192597456066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2336386.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.51960178298785 #Duplication Level Percentage of deduplicated Percentage of total 1 73.3885732769918 33.40618630990291 2 14.282341878375659 13.002530296643016 3 5.114584488691216 6.984415476320121 4 2.3381874946206764 4.25733454596381 5 1.2639937767620537 2.8768246687191765 6 0.7509253296850051 2.0509093185612177 7 0.49334950915846437 1.5719951237708072 8 0.33932242045736805 1.2356657164207172 9 0.2612636287650981 1.070335470758907 >10 1.4707710795536306 12.694859585451896 >50 0.16888990187010772 5.374457238688582 >100 0.11656160964149945 9.875031715027415 >500 0.008686350414516753 2.7044446524730543 >1k 0.0021715876036291805 1.5258971322093102 >5k 2.832505569951105E-4 0.9131572884196488 >10k+ 9.441685233170349E-5 0.45595546066946174 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10609 0.4540773656407803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9120 0.3903464581623071 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6769 0.2897209622040194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5358 0.22932854417035542 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3066 0.13122831586903877 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2764 0.11830236955708516 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2514 0.10760208287500439 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.1400573364161574E-5 0.0 20-21 0.0 0.0 0.0 6.420172009248472E-5 0.0 22-23 0.0 0.0 0.0 2.99608027098262E-4 0.0 24-25 0.0 0.0 0.0 5.136137607398778E-4 0.0 26-27 0.0 0.0 0.0 7.276194943814935E-4 0.0 28-29 0.0 0.0 0.0 0.0013268355485780174 0.0 30-31 0.0 0.0 0.0 0.0028462762574334895 0.0 32-33 0.0 0.0 0.0 0.005114737034034616 0.0 34-35 0.0 0.0 0.0 0.006869584049895865 0.0 36-37 0.0 0.0 0.0 0.010229474068069231 0.0 38-39 0.0 0.0 0.0 0.015130205368462232 0.0 40-41 0.0 0.0 0.0 0.020993962470242503 0.0 42-43 0.0 0.0 0.0 0.026964722438843583 0.0 44-45 0.0 0.0 0.0 0.02978959812291291 0.0 46-47 0.0 0.0 0.0 0.034454923116300135 0.0 48-49 0.0 0.0 0.0 0.04123890487273935 0.0 50-51 0.0 0.0 0.0 0.048878909563745035 0.0 52-53 0.0 0.0 0.0 0.06206166275606856 0.0 54-55 0.0 0.0 0.0 0.07310435861197592 0.0 56-57 0.0 0.0 0.0 0.09932006098307386 0.0 58-59 0.0 0.0 0.0 0.14832737398700385 0.0 60-61 0.0 0.0 0.0 0.18849625019153513 0.0 62-63 0.0 0.0 0.0 0.21120225853091057 0.0 64-65 0.0 0.0 0.0 0.25304037945784646 0.0 66-67 0.0 0.0 0.0 0.34138194630510543 0.0 68-69 0.0 0.0 0.0 0.4620811800789767 0.0 70-71 2.1400573364161574E-5 0.0 0.0 0.5679712170848481 0.0 72-73 4.280114672832315E-5 0.0 0.0 0.6874720187503263 0.0 74-75 4.280114672832315E-5 0.0 0.0 0.8306418545565672 0.0 76-77 4.280114672832315E-5 0.0 0.0 0.9256389997200805 0.0 78-79 4.280114672832315E-5 0.0 0.0 1.0519237831419979 0.0 80-81 4.280114672832315E-5 0.0 0.0 1.1784653734442854 0.0 82-83 4.280114672832315E-5 0.0 0.0 1.2753457690638448 0.0 84-85 4.280114672832315E-5 0.0 0.0 1.4378617231912878 0.0 86-87 4.280114672832315E-5 0.0 0.0 1.7083221693675616 0.0 88-89 4.280114672832315E-5 0.0 0.0 2.076198025497499 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 950 0.0 24.999254 5 GTACATG 12865 0.0 24.859394 1 TACATGG 13365 0.0 23.563215 2 GTATCAA 20940 0.0 23.533545 1 GGTATCA 15805 0.0 22.88102 1 ACATGGG 13050 0.0 22.857557 3 CGAATCG 85 3.1529362E-6 22.352514 82-83 GAGTACT 11765 0.0 22.286734 12-13 TACCTGG 1675 0.0 21.55206 2 GTACTTT 12330 0.0 21.438845 14-15 GTAAGGT 885 0.0 20.930237 4 AGTACTT 12140 0.0 20.40492 12-13 GTATAGG 965 0.0 20.190252 1 TCAACGC 24405 0.0 20.10362 4 ATCAACG 24420 0.0 20.092564 3 CATGGGA 7225 0.0 20.05002 4 CAACGCA 24660 0.0 19.916277 5 TATCAAC 24805 0.0 19.819427 2 AACGCAG 25120 0.0 19.55115 6 CGTATAG 245 1.749795E-7 19.39631 1 >>END_MODULE