FastQCFastQC Report
Thu 26 May 2016
SRR936672_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936672_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369362
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80780.34093566116110585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62690.26458599403552513No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55950.23613951772671293No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37650.1589035360573859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.1511377324359891No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26330.11112696160400985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131700.028.6131551
TACATGG135600.027.1933082
ACATGGG132700.026.5728953
GTATCAA183400.024.0969451
CATGGGA74750.021.99834
GAGTACT84400.021.96349312-13
CATGGGG52650.021.0320594
TCAACGC210600.020.7161254
ATCAACG211100.020.6886963
TACCTGG17950.020.6485792
GTACTTT89750.020.62450214-15
TATCAAC212850.020.5834082
AGTACTT90300.020.58108512-13
CAACGCA212700.020.4449945
AACGCAG216100.020.1673096
GTACCTG18400.020.1444381
GGTATCA135950.019.3646181
ATGGGTA10150.019.197795
CATGGGT22150.018.6667964
AGAGTAC165200.018.64599410-11
Produced by FastQC (version 0.11.5)