FastQCFastQC Report
Thu 26 May 2016
SRR936672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369362
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102520.43269031916608774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91090.38444948471360646No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68590.2894872121693519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52910.22330905956962255No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32680.13792742518872167No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27020.11403913796203366No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24670.10412085616296708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG124750.024.1499231
GTATCAA209150.024.0605281
GTATAGG8800.023.7594851
GAGTACT113400.022.76176612-13
ACATGGG125900.022.4464363
TACATGG132900.022.3033832
GGTATCA156000.022.0841391
GTACTTT119550.021.610714-15
AGTACTT117500.021.07831812-13
TAAGGTG10300.020.748455
TCAACGC244150.020.540814
ATCAACG245550.020.4258523
CAACGCA246200.020.3890635
TATCAAC249550.020.1580962
AACGCAG250150.020.085676
GTAAGGT9250.019.5097714
AGAGTAC197800.019.3272810-11
ACTTTTT134950.019.07418316-17
CATGGGG50400.019.0340484
TACACCG1759.957078E-518.9963575
Produced by FastQC (version 0.11.5)