##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936672_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2369362 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.971016670310405 31.0 31.0 34.0 30.0 34.0 2 32.136909429627046 33.0 31.0 34.0 30.0 34.0 3 32.2181798306886 34.0 31.0 34.0 30.0 34.0 4 35.904045477221295 37.0 35.0 37.0 35.0 37.0 5 35.75230800527737 37.0 35.0 37.0 35.0 37.0 6 35.84265426726689 37.0 35.0 37.0 35.0 37.0 7 35.789864950986804 37.0 35.0 37.0 33.0 37.0 8 35.760796788333735 37.0 35.0 37.0 33.0 37.0 9 37.40442532631147 39.0 37.0 39.0 34.0 39.0 10-11 37.299789774631314 39.0 37.0 39.0 34.0 39.0 12-13 37.20124235975761 39.0 37.0 39.0 33.0 39.0 14-15 38.34964222436251 40.0 38.0 41.0 33.5 41.0 16-17 38.25888340405561 40.0 38.0 41.0 33.0 41.0 18-19 38.40896410088454 40.0 38.0 41.0 33.5 41.0 20-21 38.434431716217276 40.0 38.0 41.0 34.0 41.0 22-23 38.353247836337374 40.0 38.0 41.0 34.0 41.0 24-25 38.222344453907844 40.0 38.0 41.0 33.5 41.0 26-27 38.03932619836057 40.0 37.0 41.0 33.0 41.0 28-29 37.95989679922275 40.0 37.0 41.0 33.0 41.0 30-31 37.92183739757792 40.0 37.0 41.0 33.0 41.0 32-33 37.758212337329624 40.0 37.0 41.0 33.0 41.0 34-35 37.585894852707185 40.0 36.5 41.0 33.0 41.0 36-37 37.3855588550842 40.0 36.0 41.0 32.0 41.0 38-39 37.217427729490055 39.0 36.0 41.0 32.0 41.0 40-41 37.01959979099858 39.0 36.0 41.0 31.0 41.0 42-43 36.73098391043665 39.0 35.0 41.0 31.0 41.0 44-45 36.43151236493199 39.0 35.0 41.0 30.0 41.0 46-47 36.01775119209306 39.0 35.0 40.0 29.5 41.0 48-49 35.60514497151554 39.0 35.0 40.0 28.0 41.0 50-51 35.30427347108631 38.5 35.0 40.0 27.0 41.0 52-53 34.95653682299286 38.0 34.0 40.0 26.0 41.0 54-55 34.75663427538721 38.0 34.0 40.0 26.0 41.0 56-57 34.46610754287441 38.0 34.0 40.0 25.5 41.0 58-59 34.104315423308044 37.5 33.0 40.0 24.5 41.0 60-61 33.69257188221977 37.0 33.0 40.0 23.0 41.0 62-63 33.38490234924001 36.5 32.5 39.0 22.5 40.5 64-65 32.97695455569896 36.0 32.0 39.0 22.0 40.0 66-67 32.561772325208224 35.5 31.5 39.0 21.5 40.0 68-69 32.210046206531544 35.0 31.0 38.0 20.0 40.0 70-71 31.85214247548496 35.0 31.0 37.5 19.5 40.0 72-73 31.40712710847899 35.0 31.0 37.0 18.0 39.0 74-75 30.917796436340247 34.0 30.0 36.5 16.0 39.0 76-77 29.42183233292338 32.5 28.5 35.0 13.0 37.0 78-79 30.18471090529856 34.0 30.0 35.0 12.0 37.0 80-81 29.960814345802795 34.0 30.0 35.0 8.0 37.0 82-83 29.678834006791703 34.0 29.5 35.0 7.0 36.5 84-85 29.31454501253924 34.0 29.0 35.0 4.0 36.0 86-87 28.95383968342533 34.0 29.0 35.0 2.0 36.0 88-89 28.515161676434417 33.5 29.0 35.0 2.0 35.0 90-91 28.072850623923234 33.0 27.5 35.0 2.0 35.0 92-93 27.48268711155155 33.0 26.5 35.0 2.0 35.0 94-95 26.981213719136207 33.0 25.5 34.5 2.0 35.0 96-97 26.308970938168166 32.0 25.0 34.0 2.0 35.0 98-99 25.489680766383525 32.0 22.5 34.0 2.0 35.0 100-101 22.34820892712891 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2660995815836422 1101 2 0.22424813258190568 1101 3 0.2046300534180645 1101 4 0.16315244548674457 1101 5 0.12150603290139372 1101 6 0.09597625205923777 1101 7 0.23898039800426574 1101 8 0.15666015564820412 1101 9 0.13791198732368315 1101 10-11 0.22184027327762124 1101 12-13 0.1757068535833355 1101 14-15 0.33094095685211045 1101 16-17 0.2911213199746072 1101 18-19 0.2119373891535261 1101 20-21 0.09162268155619557 1101 22-23 0.10412813798319576 1101 24-25 0.1422185411551098 1101 26-27 0.2375921932730023 1101 28-29 0.21156847922630817 1101 30-31 0.1840452558199246 1101 32-33 0.1957652873292801 1101 34-35 0.20149661413798015 1101 36-37 0.3311368308303173 1101 38-39 0.2691331122328293 1101 40-41 0.28667391066834824 1101 42-43 0.4858357184482571 1101 44-45 0.3589856297342493 1101 46-47 0.32805004720988507 1101 48-49 0.22366720945066731 1101 50-51 0.24182198748106742 1101 52-53 0.39641227167071946 1101 54-55 0.31105633081525497 1101 56-57 0.29949504763548873 1101 58-59 0.15525889953551086 1101 60-61 0.22763531747968102 1101 62-63 0.2962329809909434 1101 64-65 0.2909159745448804 1101 66-67 0.035618393505856716 1101 68-69 -0.06322031957149932 1101 70-71 0.04145101103169324 1101 72-73 0.24722715414065277 1101 74-75 -0.010835371869063692 1101 76-77 -0.2127851038148023 1101 78-79 -0.028012650200377465 1101 80-81 0.1918312844909913 1101 82-83 0.04465807016527279 1101 84-85 0.10237147190965246 1101 86-87 0.17839462390292837 1101 88-89 0.11900320041121049 1101 90-91 0.1420290226236922 1101 92-93 0.05442195844296194 1101 94-95 0.04192221592342804 1101 96-97 0.16564142673281168 1101 98-99 0.10199579716559981 1101 100-101 0.9020695776628962 1102 1 0.1993793214663775 1102 2 0.2810123888227025 1102 3 0.23760144723868137 1102 4 0.19725764226736686 1102 5 0.20446675540095782 1102 6 0.18135213290806007 1102 7 0.16980287861957777 1102 8 0.19691261772184987 1102 9 0.22994990683709204 1102 10-11 0.2536784617853485 1102 12-13 0.28231740162971874 1102 14-15 0.43160803726764385 1102 16-17 0.3511191126646409 1102 18-19 0.30545289152173893 1102 20-21 0.20990089478260643 1102 22-23 0.291537320525471 1102 24-25 0.33568852937615645 1102 26-27 0.25704427837742116 1102 28-29 0.2676741283593742 1102 30-31 0.3201833558625964 1102 32-33 0.26982465387381893 1102 34-35 0.24641112204796656 1102 36-37 0.2773927510229086 1102 38-39 0.21847196180466 1102 40-41 0.22819812593446187 1102 42-43 0.2723148027254041 1102 44-45 0.36245530319447994 1102 46-47 0.23530969313451777 1102 48-49 0.4003299666198785 1102 50-51 0.3096959057232027 1102 52-53 0.35766315768255197 1102 54-55 0.24309874197867032 1102 56-57 0.14644904620671895 1102 58-59 0.27906873513342134 1102 60-61 0.3911163037355294 1102 62-63 0.3763672311237869 1102 64-65 0.09800989956831785 1102 66-67 0.10646905915855598 1102 68-69 0.1571337611031538 1102 70-71 0.29043252075999604 1102 72-73 0.4495822954397042 1102 74-75 0.3831489381679418 1102 76-77 0.06731542574037519 1102 78-79 0.27987993056864013 1102 80-81 0.3825086670144877 1102 82-83 0.5203903439189901 1102 84-85 0.5982783861155525 1102 86-87 0.512230002938022 1102 88-89 0.3854613059469223 1102 90-91 0.31363561889461167 1102 92-93 0.22893617427617485 1102 94-95 0.296957558780953 1102 96-97 0.5717180634192829 1102 98-99 0.6608819662436822 1102 100-101 1.2786688647097435 1103 1 0.17860805475497799 1103 2 0.1918900251407294 1103 3 0.10272377391874699 1103 4 0.1236872355238745 1103 5 0.14741212371415457 1103 6 0.07515597272679742 1103 7 0.11914930518738487 1103 8 0.07025766651267418 1103 9 0.18102168346744207 1103 10-11 0.09289360231370125 1103 12-13 0.18979215462836407 1103 14-15 0.2348952079982709 1103 16-17 0.18200909564920664 1103 18-19 0.11800611577562137 1103 20-21 0.12094714825765607 1103 22-23 0.08623161401482093 1103 24-25 0.10599944880286927 1103 26-27 0.01055762327929699 1103 28-29 -0.038988582367849745 1103 30-31 0.003076244041196219 1103 32-33 0.08691686970595214 1103 34-35 0.11780765301013219 1103 36-37 -0.005822924400689544 1103 38-39 0.020584389929808822 1103 40-41 0.07897235007788339 1103 42-43 0.029852105275324448 1103 44-45 0.026553670049914047 1103 46-47 0.029299045835095683 1103 48-49 -0.15152079542900765 1103 50-51 -0.05225670408132288 1103 52-53 0.009475802323279936 1103 54-55 0.030579703680061243 1103 56-57 -0.04304800261893149 1103 58-59 0.10223416141990072 1103 60-61 -0.07236764097273607 1103 62-63 0.1332801264524548 1103 64-65 0.11520871354829154 1103 66-67 -0.07665992024715962 1103 68-69 0.09856824078828907 1103 70-71 -0.05358470580541663 1103 72-73 0.0037030974234397718 1103 74-75 0.008822794632092013 1103 76-77 -0.10814198315738466 1103 78-79 -0.23306121517371636 1103 80-81 -0.10144848858343991 1103 82-83 0.0013486443995240904 1103 84-85 0.2401916596255056 1103 86-87 0.21947109976168022 1103 88-89 0.19611287692284307 1103 90-91 0.2060726326663982 1103 92-93 0.38819837796842194 1103 94-95 0.14641052908202568 1103 96-97 0.2862185403630164 1103 98-99 0.3219497134749325 1103 100-101 0.685939427024497 1104 1 0.12334653809051943 1104 2 0.1916527603294682 1104 3 0.09746327410347533 1104 4 0.16578140253796647 1104 5 0.10709151116420657 1104 6 6.048269072280732E-4 1104 7 0.12918645407783202 1104 8 0.07726460481365649 1104 9 0.16936350749152496 1104 10-11 0.14895334559317064 1104 12-13 0.15884985743046087 1104 14-15 0.21636016568039196 1104 16-17 0.27806236498882697 1104 18-19 0.15494739951382996 1104 20-21 0.14280220318445913 1104 22-23 0.13248935477929535 1104 24-25 0.14231960716018222 1104 26-27 0.20179517958731452 1104 28-29 0.21814376733335905 1104 30-31 0.14884877826663967 1104 32-33 0.009074522004546282 1104 34-35 -0.0966335544643826 1104 36-37 0.10776972242385341 1104 38-39 0.06380980166899519 1104 40-41 0.13525876598954056 1104 42-43 0.10167004520664591 1104 44-45 0.1056810609165737 1104 46-47 -0.03029043519897101 1104 48-49 0.134677764712535 1104 50-51 0.11098190974986011 1104 52-53 0.03805138302891464 1104 54-55 0.05041461821387827 1104 56-57 -0.09697448103291606 1104 58-59 0.033028252848197326 1104 60-61 0.03745419010149931 1104 62-63 0.12381961579079359 1104 64-65 0.231343907037747 1104 66-67 0.05504747122961362 1104 68-69 -0.04058884207475444 1104 70-71 0.09149764203157318 1104 72-73 0.21452940602361537 1104 74-75 0.001345563580233744 1104 76-77 -0.10154390296866111 1104 78-79 -0.06093576309951487 1104 80-81 0.12313494219793952 1104 82-83 -0.06667178749022185 1104 84-85 0.13167054814154966 1104 86-87 0.14653208359716885 1104 88-89 0.1528442490930395 1104 90-91 0.17164508486601093 1104 92-93 0.2621819524644877 1104 94-95 0.3309245938010861 1104 96-97 0.4151507809224171 1104 98-99 0.585667432749986 1104 100-101 1.19025722290219 1105 1 0.02746048088339137 1105 2 0.08739655909887034 1105 3 0.09405956799556492 1105 4 0.1199033495669326 1105 5 0.21449956360162759 1105 6 0.12300044492514672 1105 7 -0.02989759157387084 1105 8 0.10872359820326949 1105 9 0.08134417770116187 1105 10-11 0.14236011266169157 1105 12-13 0.14803387687049252 1105 14-15 0.1035494004812918 1105 16-17 0.10611539466547981 1105 18-19 0.17611537881657569 1105 20-21 0.1711177014379004 1105 22-23 0.1638628816919976 1105 24-25 0.07971431334021162 1105 26-27 -0.011624581904044362 1105 28-29 0.08563613105757639 1105 30-31 0.009631510461318271 1105 32-33 0.17211025608094843 1105 34-35 0.21352370385313435 1105 36-37 0.2205215553352815 1105 38-39 0.18338106781013863 1105 40-41 0.2829356024339731 1105 42-43 0.3143099067714843 1105 44-45 0.5162403195308016 1105 46-47 0.511429519742741 1105 48-49 0.6095805264378669 1105 50-51 0.5260996247955347 1105 52-53 0.6186027312296005 1105 54-55 0.5664389229448261 1105 56-57 0.46396560916573293 1105 58-59 0.5047083566628459 1105 60-61 0.6289858549180991 1105 62-63 0.36456812621126033 1105 64-65 0.39327678638723285 1105 66-67 0.28353894539879576 1105 68-69 0.25821984612770876 1105 70-71 0.4222235881293521 1105 72-73 0.5007673388314231 1105 74-75 0.4586947130495176 1105 76-77 0.332948461341946 1105 78-79 0.19872707971194714 1105 80-81 0.1678853654255903 1105 82-83 0.4541254461519628 1105 84-85 0.581989017051395 1105 86-87 0.7116915496765053 1105 88-89 0.5577769402110313 1105 90-91 0.6884964939927158 1105 92-93 0.47683210870834714 1105 94-95 0.6108787614866102 1105 96-97 0.6140975348675113 1105 98-99 0.47399411934221547 1105 100-101 0.9378225277932195 1106 1 0.10257322093893251 1106 2 0.12857801556458526 1106 3 0.0551639756673552 1106 4 0.08361751977046339 1106 5 0.13152381929872803 1106 6 -0.0068440994551792755 1106 7 0.022605167416067218 1106 8 0.09570647295371515 1106 9 0.11872954498633703 1106 10-11 0.010865559618068232 1106 12-13 0.11950638921376111 1106 14-15 0.11375032675264407 1106 16-17 0.13737432138327677 1106 18-19 0.11602231461289136 1106 20-21 0.016302464008667528 1106 22-23 0.06736527697916017 1106 24-25 -5.421247722381395E-5 1106 26-27 -7.262984874785161E-4 1106 28-29 -0.12004036390912631 1106 30-31 -0.12056026860719271 1106 32-33 -0.18283407241914063 1106 34-35 -0.12220018174127034 1106 36-37 -0.3433395788866562 1106 38-39 -0.11366084230063933 1106 40-41 -0.011244060493162067 1106 42-43 -0.04396035092676698 1106 44-45 -0.16750567856795584 1106 46-47 -0.07698116156777246 1106 48-49 -0.07840549676106434 1106 50-51 0.008078776665641385 1106 52-53 -0.0857059759397174 1106 54-55 0.02077806875841759 1106 56-57 -0.2667124857809853 1106 58-59 -0.26466318679494805 1106 60-61 -0.1270300699562057 1106 62-63 -0.11234978154561048 1106 64-65 -0.2807993227111112 1106 66-67 -0.18312441724408757 1106 68-69 -0.13391387161708224 1106 70-71 -0.09803691983698926 1106 72-73 -0.06267296162140923 1106 74-75 -0.10904961170428251 1106 76-77 -0.17761780670855742 1106 78-79 -0.313251775576493 1106 80-81 -0.12838582260249964 1106 82-83 0.0278131113206328 1106 84-85 0.28878253221530414 1106 86-87 0.2270384019304501 1106 88-89 0.1739417334701976 1106 90-91 0.24315243585022372 1106 92-93 0.4804460343100594 1106 94-95 0.3877965131478547 1106 96-97 0.7819334877453663 1106 98-99 0.8805579398135315 1106 100-101 1.1269743510680108 1107 1 0.02425108637010709 1107 2 0.026000661931178115 1107 3 -0.013817889905588743 1107 4 0.058940071574483 1107 5 -0.1151515894387245 1107 6 -0.10793309942778961 1107 7 -0.10880136626158077 1107 8 -0.034783915332766924 1107 9 -0.1897664268414303 1107 10-11 -0.010489552640756017 1107 12-13 -0.10964006678404559 1107 14-15 -0.04868875932506711 1107 16-17 -0.01811183816811024 1107 18-19 -0.12680438135152627 1107 20-21 -0.05924408993468688 1107 22-23 -0.21119511660734247 1107 24-25 -0.04851938300398473 1107 26-27 -0.08551130959496334 1107 28-29 -0.09765883695718003 1107 30-31 -0.13624702324229077 1107 32-33 -0.05037712094246416 1107 34-35 0.04381198412824716 1107 36-37 0.012068369202197005 1107 38-39 -0.09856892973638764 1107 40-41 -0.13801155094427742 1107 42-43 -0.16800767731906774 1107 44-45 -0.08917822787500285 1107 46-47 -0.23652602079518914 1107 48-49 -0.2502519477649301 1107 50-51 -0.3548397271514929 1107 52-53 -0.1505354652857136 1107 54-55 -0.2574906629271254 1107 56-57 -0.42532347920348457 1107 58-59 -0.3023181800048107 1107 60-61 -0.10526376355092282 1107 62-63 -0.2741096119451356 1107 64-65 -0.23881740719883027 1107 66-67 -0.1570895853544343 1107 68-69 0.004568826009830218 1107 70-71 0.045628596076745964 1107 72-73 -0.10364062508820027 1107 74-75 -0.04861897343108623 1107 76-77 -0.231565834345961 1107 78-79 -0.15406438542292378 1107 80-81 0.0520089559970387 1107 82-83 -0.13944141664781995 1107 84-85 -0.04299677452988959 1107 86-87 0.02803259768947086 1107 88-89 -0.018765752438046235 1107 90-91 -0.03274457954881527 1107 92-93 0.10423488430353345 1107 94-95 0.31701248149597205 1107 96-97 0.4779124036748925 1107 98-99 0.491190188066863 1107 100-101 1.0973302736330233 1108 1 0.11285308192301713 1108 2 0.1748312928037805 1108 3 0.13976857294800737 1108 4 0.07342301943511842 1108 5 0.08170978356919534 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-0.7080405928246805 2316 56-57 -0.6175672278345417 2316 58-59 -1.1953760575278523 2316 60-61 -0.6838205811843139 2316 62-63 -0.5980679027405742 2316 64-65 -0.46235636201027575 2316 66-67 -0.370480081367937 2316 68-69 -0.9760768961320281 2316 70-71 -0.7746030658112382 2316 72-73 -0.46397484815960155 2316 74-75 -0.618934267397325 2316 76-77 -0.48257075987319453 2316 78-79 -0.6027795657072836 2316 80-81 -0.35707957190926365 2316 82-83 -0.5511039769897579 2316 84-85 -0.2978547567425558 2316 86-87 -0.4769058198142382 2316 88-89 -0.6612932726510188 2316 90-91 -0.7146304282586975 2316 92-93 -0.8352775705856743 2316 94-95 -0.9433662491031356 2316 96-97 -0.9287271789855716 2316 98-99 -1.3191999065951627 2316 100-101 -2.036930354859468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 26.0 5 61.0 6 148.0 7 374.0 8 867.0 9 1675.0 10 2576.0 11 4377.0 12 6512.0 13 8974.0 14 11998.0 15 16420.0 16 21250.0 17 27668.0 18 24065.0 19 15063.0 20 13215.0 21 13638.0 22 14217.0 23 16022.0 24 18904.0 25 22583.0 26 27739.0 27 34547.0 28 43622.0 29 53854.0 30 66969.0 31 83909.0 32 105292.0 33 135345.0 34 180192.0 35 247699.0 36 356607.0 37 477494.0 38 297880.0 39 17574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.777069708774256 19.345902965278164 14.744537934318439 22.132489391629136 2 15.835872689305663 20.880323202783913 39.209400942745134 24.074403165165283 3 19.795207436994776 24.993911264435155 29.780227248073025 25.430654050497036 4 13.00079937130755 17.009389025400086 36.85397165988144 33.13583994341093 5 13.516732628678682 38.023579185858395 33.60918214842993 14.850506037032988 6 31.923066209384636 36.42326499707516 16.949963745514616 14.703705048025586 7 29.109861642079178 33.19767093420085 20.01213828870388 17.680329135016095 8 27.290215678313402 33.86130950019457 19.20170915208398 19.64676566940805 9 26.977220028007537 16.78245029674655 20.330114182636507 35.9102154926094 10-11 25.440435020060253 26.728503284850518 26.50397026710144 21.32709142798779 12-13 26.718399921328086 24.141430490895434 27.344926326327634 21.795243261448842 14-15 23.21081204731917 25.327145719281646 26.42505962588162 25.036982607517565 16-17 22.13997315310475 28.907999053753514 26.890071439077573 22.061956354064165 18-19 21.888737439188866 26.842851978634407 29.954436361265145 21.31397422091158 20-21 22.830428613272264 25.731673758589864 30.1022595956211 21.335638032516773 22-23 22.454284566898593 25.73989963950158 29.965326057664974 21.840489735934852 24-25 22.610116140969595 26.051063535247042 30.0201699866884 21.318650337094965 26-27 22.12013950835271 26.240498066850815 30.149192563859557 21.490169860936923 28-29 21.66699974950201 26.55061315027917 30.21406709758511 21.568320002633712 30-31 21.847096471398594 26.06325686145078 30.14819838907638 21.94144827807425 32-33 21.786021959691183 26.293971827133593 30.09768445258423 21.822321760590995 34-35 22.6385080060144 26.13283389168799 29.64028594792793 21.588372154369676 36-37 22.480266149786278 25.85573845908728 29.738566712073077 21.925428679053365 38-39 22.0345786101195 26.330272828644098 29.8638304733076 21.771318087928805 40-41 22.232215567573363 26.252086802544746 29.843550215892133 21.67214741398976 42-43 22.12382171584089 26.41445803677061 29.182299389379413 22.279420858009082 44-45 22.266161233214852 26.39209275068221 29.12291904911938 22.218826966983556 46-47 22.78185413329788 26.795857944461677 28.38464476619164 22.037643156048798 48-49 22.972704053800534 26.9818086816839 28.04697670600455 21.998510558511015 50-51 22.584772454135774 27.031758609665278 28.135813189862652 22.247655746336296 52-53 23.020888772026527 27.273431337336383 27.6735606550126 22.032119235624485 54-55 22.447815482247535 27.10336622163284 27.85231117787158 22.596507118248045 56-57 22.726639555665493 27.360316011728024 27.664090643158833 22.248953789447647 58-59 23.19535097943774 26.82719248956309 27.616551634464827 22.360904896534343 60-61 22.736232625554138 27.136777438026314 28.269587192474443 21.857402743945105 62-63 22.587740681410942 27.126786841166627 28.21595530707392 22.069517170348515 64-65 22.401284622750765 27.227189155339016 27.91990272461005 22.45162349730017 66-67 22.445432116690046 27.498212039364045 27.46702164904311 22.5893341949028 68-69 22.46591664000662 27.109154540213343 27.906592474353598 22.518336345426444 70-71 22.614105300698604 27.27372293965769 27.691030163240455 22.421141596403242 72-73 22.752215207436773 27.0381607188944 27.894763164997478 22.31486090867135 74-75 22.47203862679058 27.78486835996522 27.33263271628386 22.410460296960334 76-77 22.710295343174238 27.26943520073303 27.59767166097905 22.42259779511368 78-79 22.631752338337165 27.307582482589233 27.482286379743968 22.578378799329634 80-81 22.716742753783777 27.802760242212415 27.115527892629576 22.364969111374233 82-83 22.792725252663402 27.64246534980299 27.148641472509897 22.416167925023704 84-85 22.51842057379565 27.600295185130303 27.019001698412154 22.862282542661895 86-87 22.504610402520115 27.608829348203685 27.226299956228083 22.66026029304812 88-89 22.552535948482376 27.56979582237372 26.879928659328662 22.997739569815245 90-91 22.5610984267712 27.494682641193453 27.136011640972608 22.80820729106274 92-93 22.77093626735128 27.14696982938231 27.363677469118215 22.718416434148192 94-95 22.415474580629034 26.985228026430725 27.462808308849883 23.136489084090353 96-97 22.423974083760733 27.025445184632936 27.59993758684476 22.95064314476157 98-99 22.140103207716084 27.298820965862063 27.077773179507105 23.483302646914748 100-101 21.83483902192429 27.607746847514004 27.214099183078684 23.343314947483027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 459.0 1 358.5 2 302.0 3 357.5 4 317.0 5 299.0 6 507.5 7 1025.5 8 1596.5 9 1999.5 10 2042.0 11 1939.5 12 2090.0 13 2394.0 14 2768.0 15 3070.5 16 3384.5 17 3712.0 18 4003.0 19 4295.0 20 4725.5 21 5354.5 22 6089.5 23 6999.5 24 7912.5 25 9375.5 26 12101.0 27 15250.5 28 17880.0 29 21276.0 30 25576.0 31 30232.0 32 36329.5 33 42701.0 34 49110.0 35 54497.0 36 61363.0 37 68038.0 38 70620.0 39 70616.5 40 69881.0 41 72547.0 42 75949.0 43 79003.5 44 80923.0 45 81899.5 46 81227.5 47 85386.5 48 100653.0 49 101131.5 50 88835.5 51 82165.0 52 77504.5 53 76595.5 54 75353.0 55 70228.0 56 63826.5 57 57226.0 58 52305.0 59 47438.5 60 41156.0 61 34515.0 62 28468.5 63 22526.5 64 16801.5 65 12933.5 66 9625.5 67 7573.5 68 5540.0 69 3564.0 70 2833.5 71 2313.5 72 1932.0 73 1676.5 74 1387.0 75 1178.5 76 871.5 77 539.5 78 355.5 79 255.0 80 171.5 81 108.5 82 80.5 83 59.5 84 37.5 85 15.0 86 9.0 87 5.5 88 4.5 89 3.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020174207233846074 2 0.013632361791908538 3 0.009833870890138359 4 0.0 5 0.0029121763580238057 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001202855452227224 14-15 2.1102727232056564E-4 16-17 2.1102727232056562E-5 18-19 0.0016249099968683551 20-21 0.0 22-23 1.4771909062439594E-4 24-25 0.0 26-27 0.0017515263602606948 28-29 0.0034397445388252195 30-31 0.0016038072696362986 32-33 0.008567707256214965 34-35 0.0020891699959735997 36-37 8.230063620502059E-4 38-39 0.0015193963607080725 40-41 3.165409084808484E-4 42-43 0.005613325443727045 44-45 0.0017937318147248076 46-47 2.954381812487919E-4 48-49 7.385954531219797E-4 50-51 1.8992454508850906E-4 52-53 1.2661636339233938E-4 54-55 0.0012239581794592806 56-57 0.01418103269994201 58-59 0.025534299950788444 60-61 0.019984282688757564 62-63 0.012957074520482729 64-65 0.01762077723876723 66-67 0.0017937318147248076 68-69 0.001012930907138715 70-71 1.8992454508850906E-4 72-73 6.330818169616969E-5 74-75 9.285199982104888E-4 76-77 0.0014138827245477896 78-79 0.008884248164695813 80-81 0.015489401788329518 82-83 0.01688218178564525 84-85 0.02865750358113281 86-87 0.011100034524061752 88-89 0.04252199537259397 90-91 0.049591408995332924 92-93 0.03093659812219492 94-95 0.031865118120405406 96-97 0.05366423535111983 98-99 0.05579561080155755 100-101 0.06366692805911464 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2369362.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.101742087943094 #Duplication Level Percentage of deduplicated Percentage of total 1 73.19072718267185 33.01029300621872 2 14.296936151522472 12.896334539075124 3 5.11565501468249 6.921748592493072 4 2.3640088980645713 4.264836784564434 5 1.2962601194822152 2.9231794793886583 6 0.7988683247767284 2.1618211887784295 7 0.504662620173361 1.5932814335539163 8 0.37440808532530395 1.350916551998596 9 0.2537504431309393 1.0300128336713605 >10 1.5032004113908608 12.817613566175279 >50 0.17011350209127693 5.311333341280966 >100 0.1199791670535968 10.082131801864866 >500 0.008525715137175672 2.5939776336255354 >1k 0.0025296077879531998 1.7110077269297665 >5k 2.8106753199479996E-4 0.8983086354539144 >10k+ 9.36891773316E-5 0.433202884927491 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10252 0.43269031916608774 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9109 0.38444948471360646 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6859 0.2894872121693519 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5291 0.22330905956962255 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3268 0.13792742518872167 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2702 0.11403913796203366 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2467 0.10412085616296708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.2205454464113125E-5 0.0 16-17 0.0 0.0 0.0 1.0551363616028281E-4 0.0 18-19 0.0 0.0 0.0 1.688218178564525E-4 0.0 20-21 0.0 0.0 0.0 2.5323272678467876E-4 0.0 22-23 0.0 0.0 0.0 3.37643635712905E-4 0.0 24-25 0.0 0.0 0.0 4.2205454464113123E-4 0.0 26-27 0.0 0.0 0.0 7.385954531219797E-4 0.0 28-29 0.0 0.0 0.0 0.001498293633476016 0.0 30-31 0.0 0.0 0.0 0.0034186418115931634 0.0 32-33 0.0 0.0 0.0 0.0050013463539974054 0.0 34-35 0.0 0.0 0.0 0.0066051536236337045 0.0 36-37 0.0 0.0 0.0 0.00939071361826517 0.0 38-39 0.0 0.0 0.0 0.014307649063334349 0.0 40-41 0.0 0.0 0.0 0.019984282688757564 0.0 42-43 0.0 0.0 0.0 0.025829738132037233 0.0 44-45 0.0 0.0 0.0 0.028868530853453377 0.0 46-47 0.0 0.0 0.0 0.03332120629941731 0.0 48-49 0.0 0.0 0.0 0.038892326288680244 0.0 50-51 0.0 0.0 0.0 0.04691136263686174 0.0 52-53 0.0 0.0 0.0 0.061155703518499915 0.0 54-55 0.0 0.0 0.0 0.07134832077158323 0.0 56-57 0.0 0.0 0.0 0.09964707798977109 0.0 58-59 0.0 0.0 0.0 0.14615748880922375 0.0 60-61 0.0 0.0 0.0 0.1853452532791528 0.0 62-63 0.0 0.0 0.0 0.20682782960138635 0.0 64-65 0.0 0.0 0.0 0.24749278497755936 0.0 66-67 0.0 0.0 0.0 0.3312073039071277 0.0 68-69 0.0 0.0 0.0 0.44691355732049387 0.0 70-71 0.0 0.0 0.0 0.5470037925821382 0.0 72-73 0.0 0.0 0.0 0.6628999705405928 0.0 74-75 0.0 0.0 0.0 0.7984849930065563 0.0 76-77 0.0 0.0 0.0 0.8893955419222559 0.0 78-79 0.0 0.0 0.0 1.0115381271413992 0.0 80-81 0.0 0.0 0.0 1.1348202596310737 0.0 82-83 0.0 0.0 0.0 1.2343407212574524 0.0 84-85 0.0 0.0 0.0 1.401748656389357 0.0 86-87 0.0 0.0 0.0 1.6794183413087573 0.0 88-89 0.0 0.0 0.0 2.043398180607269 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12475 0.0 24.149923 1 GTATCAA 20915 0.0 24.060528 1 GTATAGG 880 0.0 23.759485 1 GAGTACT 11340 0.0 22.761766 12-13 ACATGGG 12590 0.0 22.446436 3 TACATGG 13290 0.0 22.303383 2 GGTATCA 15600 0.0 22.084139 1 GTACTTT 11955 0.0 21.6107 14-15 AGTACTT 11750 0.0 21.078318 12-13 TAAGGTG 1030 0.0 20.74845 5 TCAACGC 24415 0.0 20.54081 4 ATCAACG 24555 0.0 20.425852 3 CAACGCA 24620 0.0 20.389063 5 TATCAAC 24955 0.0 20.158096 2 AACGCAG 25015 0.0 20.08567 6 GTAAGGT 925 0.0 19.509771 4 AGAGTAC 19780 0.0 19.32728 10-11 ACTTTTT 13495 0.0 19.074183 16-17 CATGGGG 5040 0.0 19.034048 4 TACACCG 175 9.957078E-5 18.996357 5 >>END_MODULE