FastQCFastQC Report
Thu 26 May 2016
SRR936671_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936671_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316362
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.38925462752647066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41170.31275591364685396No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37360.28381250750173587No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32430.24636080348718667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23440.1780665196959499No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20850.15839108087289058No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20830.15823914698236502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG11650.028.541651
TACCTGG12400.027.198422
TAACGCG350.00836737927.1602176-77
GTACATG54750.026.6355231
TACATGG55650.025.7779712
ACATGGG56550.024.3606513
GTATCAA101850.023.9256271
GAGTACT52250.023.18517512-13
GTCCGAT1452.3341196E-522.9317421
GTACTTT56700.021.2825514-15
TCAACGC113750.021.1319274
CATGGGG26600.021.0736794
CAACGCA114400.021.0541865
ATCAACG114400.021.011063
AACGCAG116650.020.7303226
TATCAAC117150.020.7198032
GGTATCA75700.020.7073751
GTGTAGG6550.020.305971
AGTACTT55900.020.1840512-13
AGAGTAC84050.019.84003410-11
Produced by FastQC (version 0.11.5)