FastQCFastQC Report
Thu 26 May 2016
SRR936671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316362
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61850.4698555564502774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50600.38439274302965293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44290.3364576005688405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.2078455622389586No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19100.14509686545190456No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17290.1313468483593419No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14650.1112915748099687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.10969626895945037No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13300.10103603719949375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG57350.023.360571
TACATGG57450.022.3267232
ACATGGG56950.021.5192813
GAGTACT57450.021.37252212-13
GTACTTT60950.019.83425314-15
TACCTGG10700.019.5353032
GTACCTG11000.019.4351351
CATGGGG27900.019.4074924
AGTACTT60000.019.23713112-13
GTATAGG4002.1827873E-1117.8155421
AGAGTAC95250.017.75226610-11
CCGACCA1350.009408317517.590259
TAAGGTG6000.017.415675
TACTTTT69400.016.87171614-15
CATGGGA28750.016.8512124
GTAAGGT5100.016.7637484
GTATCAA131750.016.7315081
ACTTTTT72650.016.67208316-17
TACACTG12600.016.5863535
GTGTAGG5500.016.4118921
Produced by FastQC (version 0.11.5)