FastQCFastQC Report
Thu 26 May 2016
SRR936670_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936670_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409779
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53470.37927930547979505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43340.30742407143247275No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40560.28770466860408617No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32230.22861739322262564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25560.18130501305523775No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22040.15633656055310796No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21100.14966884880538012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG56400.027.5511381
TACATGG57050.027.0696852
ACATGGG57600.025.5737673
GTACCTG12650.024.4170821
TACCTGG13200.022.6789072
GAGTACT52200.022.48243912-13
GTGTAGC8550.022.2312971
CCCGAAC1100.002871468421.5989573
TAGCCCT10450.021.3716014
GTACTTT55050.021.23256514-15
GTATCAA113900.020.5680961
GTACACG2551.1868906E-820.4985621
CATGGGA30750.019.934264
AGTACTT53450.019.86766212-13
AGAGTAC83650.019.5403610-11
CATGGGG26700.019.3986154
TCAACGC126150.018.6454754
CAACGCA127050.018.4399015
ATCAACG127800.018.3675653
TATCAAC128650.018.0615312
Produced by FastQC (version 0.11.5)