FastQCFastQC Report
Thu 26 May 2016
SRR936670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409779
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63300.4490065464161404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55130.39105420069386765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49110.3483524722669298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30200.21421797317168154No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22030.15626562744940872No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18690.13257397081386516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.10661245485994614No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14800.1049809934748638No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14780.10483912726746533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG62400.022.3158591
GAGTACT61200.021.8816912-13
ACTGCGT1553.9116858E-521.448448
TACATGG64950.020.8420932
GACCGCT1604.9960014E-520.7781777
ACATGGG62900.020.7654933
GTAAGGT5750.020.6498534
AGTACTT63350.020.61433212-13
GTACCTG13750.020.6039051
GTACTTT65150.020.33634814-15
TACCTGG13300.019.6420422
AGAGTAC105000.018.65597210-11
GTATAGG4650.018.5874481
TAGCCCT9500.018.4979214
CATGGGG30100.018.303594
TACTTTT72300.017.83260214-15
GTATCAA143950.017.7459871
GTGTAGC6500.017.7295651
TACGGGC1350.00940943417.589997
ATACCGT1350.00940943417.589996
Produced by FastQC (version 0.11.5)