FastQCFastQC Report
Thu 26 May 2016
SRR936669_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936669_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441681
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54490.37796156015096266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43860.30422818917638506No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42080.2918814911204351No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33210.23035609125735856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.17916584875572336No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22060.15301581972711023No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20990.14559392819909536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG62800.026.4070431
TACATGG63650.025.4580442
GTGTAGC8800.024.8387151
ACATGGG63200.024.3611053
GTATCAA107550.023.7698171
TACCTGG12450.023.2824952
GAGTACT55650.022.71515512-13
GTACCTG14000.021.0434841
GGTATCA81850.020.9576471
GTACTTT60600.020.70214714-15
TCAACGC123200.020.675374
ATCAACG123200.020.6353633
CAACGCA123700.020.6295015
TATCAAC123550.020.6153682
AACGCAG125600.020.5051826
CATGGGG30250.019.9515914
AGTACTT58200.019.71938312-13
AGAGTAC92800.019.22786310-11
TACACCG2251.661243E-619.0083375
ACACCGT2552.6656198E-718.6343336
Produced by FastQC (version 0.11.5)