##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936669_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1441681 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.466236289442673 31.0 31.0 34.0 30.0 34.0 2 31.5175666461582 33.0 31.0 34.0 30.0 34.0 3 31.565790212952795 33.0 31.0 34.0 28.0 34.0 4 35.18348719307531 37.0 35.0 37.0 33.0 37.0 5 35.15959771960649 37.0 35.0 37.0 32.0 37.0 6 35.27992392214367 37.0 35.0 37.0 32.0 37.0 7 35.15021561635341 37.0 35.0 37.0 32.0 37.0 8 35.096807130010035 37.0 35.0 37.0 32.0 37.0 9 36.59821763621772 39.0 37.0 39.0 32.0 39.0 10-11 36.50878939238292 39.0 37.0 39.0 32.0 39.0 12-13 36.414088831024344 39.0 37.0 39.0 32.0 39.0 14-15 37.49299602339214 40.0 37.0 41.0 32.0 41.0 16-17 37.37964050299615 40.0 37.0 41.0 32.0 41.0 18-19 37.33340107832454 40.0 37.0 41.0 32.0 41.0 20-21 37.25346487884629 39.5 37.0 41.0 31.5 41.0 22-23 37.11219090769734 39.0 36.0 41.0 31.0 41.0 24-25 37.25113045118858 39.5 37.0 41.0 31.0 41.0 26-27 37.23010707639207 40.0 37.0 41.0 31.0 41.0 28-29 37.13690892784187 39.5 36.5 41.0 31.0 41.0 30-31 36.94331200868986 39.0 36.0 41.0 30.5 41.0 32-33 36.760812551459026 39.0 36.0 41.0 30.0 41.0 34-35 36.53764945227134 39.0 35.0 41.0 30.0 41.0 36-37 36.34558546585548 39.0 35.0 40.5 30.0 41.0 38-39 36.151463465218725 39.0 35.0 40.0 30.0 41.0 40-41 35.97753733315484 39.0 35.0 40.0 29.0 41.0 42-43 35.75262974264071 38.0 35.0 40.0 28.0 41.0 44-45 35.475079785333925 38.0 35.0 40.0 27.0 41.0 46-47 35.19116642308528 38.0 34.5 40.0 26.0 41.0 48-49 35.08469730821173 38.0 34.0 40.0 25.5 41.0 50-51 34.174644737636136 37.5 33.5 39.5 23.5 40.5 52-53 34.3353550473371 38.0 33.5 39.5 24.0 40.5 54-55 34.75658346055751 38.0 34.0 40.0 24.5 41.0 56-57 34.66413339705524 38.0 34.0 40.0 24.5 41.0 58-59 34.40890287102348 38.0 34.0 40.0 23.5 41.0 60-61 34.09612771479959 38.0 33.5 40.0 23.0 41.0 62-63 33.782969672209035 37.0 33.0 40.0 22.0 41.0 64-65 33.4471269996622 37.0 33.0 40.0 22.0 41.0 66-67 33.05511621502954 36.0 32.5 39.0 20.5 41.0 68-69 32.64801159202348 36.0 32.0 39.0 19.5 40.5 70-71 32.2062939027427 35.0 31.5 38.5 18.0 40.0 72-73 31.81287122463291 35.0 31.0 38.0 17.5 40.0 74-75 31.383574105506003 35.0 31.0 37.0 13.5 39.0 76-77 30.93598549193615 35.0 30.5 36.5 9.5 39.0 78-79 30.484030447789767 34.5 30.0 36.0 7.5 39.0 80-81 29.993537405292848 34.0 29.5 36.0 6.0 37.5 82-83 29.51585198112481 34.0 29.0 35.0 2.0 37.0 84-85 29.18102548344606 34.0 29.0 35.0 2.0 36.5 86-87 28.865798675296407 34.0 29.0 35.0 2.0 36.0 88-89 28.472717612287322 34.0 29.0 35.0 2.0 36.0 90-91 28.056454583226106 34.0 27.5 35.0 2.0 35.5 92-93 27.684367762355194 34.0 27.0 35.0 2.0 35.0 94-95 27.240428014241708 33.0 26.0 35.0 2.0 35.0 96-97 26.677526096272338 33.0 25.0 35.0 2.0 35.0 98-99 25.98693018774611 33.0 24.5 35.0 2.0 35.0 100-101 23.525338129586224 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43257380373565724 1101 2 0.5676474904786524 1101 3 0.5513584214432825 1101 4 0.5772135200518775 1101 5 0.5669616117647678 1101 6 0.44809649596233214 1101 7 0.5774237942937503 1101 8 0.7084315134736414 1101 9 0.8814427825775297 1101 10-11 0.6587873497189634 1101 12-13 0.747463945534335 1101 14-15 0.9748547587582195 1101 16-17 0.9922258472798475 1101 18-19 1.0652791113291897 1101 20-21 1.0951957675871 1101 22-23 0.9618588684707774 1101 24-25 0.9449797899921819 1101 26-27 0.8825965244279033 1101 28-29 0.7706251499005106 1101 30-31 0.682889723482603 1101 32-33 0.6053261054242682 1101 34-35 0.7676771965302791 1101 36-37 0.5784752943941669 1101 38-39 0.5045231232176164 1101 40-41 0.6315045597451459 1101 42-43 0.7380428711007454 1101 44-45 0.6956895320996566 1101 46-47 0.8014538859908029 1101 48-49 0.9243629094674617 1101 50-51 0.8505794780440397 1101 52-53 1.053476594302694 1101 54-55 1.164679399919315 1101 56-57 1.076201620645314 1101 58-59 1.006493554408408 1101 60-61 1.0225901466669 1101 62-63 1.096301076668695 1101 64-65 1.0800285859542953 1101 66-67 1.0886378444700426 1101 68-69 1.0010375580381563 1101 70-71 1.0932683343641187 1101 72-73 1.0160978692296077 1101 74-75 0.874357157501727 1101 76-77 0.8340303763571306 1101 78-79 0.9635185296890967 1101 80-81 1.0625718451703285 1101 82-83 1.1811734478209353 1101 84-85 1.0935710876150964 1101 86-87 1.0251976370608027 1101 88-89 1.0003617319355804 1101 90-91 0.8530803251896266 1101 92-93 0.9236101714457554 1101 94-95 0.9519976592947188 1101 96-97 0.7927877323683497 1101 98-99 0.9896061274027943 1101 100-101 1.5657804968081415 1102 1 0.4526589852763614 1102 2 0.41099282652179525 1102 3 0.3718615871382376 1102 4 0.38391751995435897 1102 5 0.4452691010087193 1102 6 0.3785226542396316 1102 7 0.4580010079268604 1102 8 0.3651985970183276 1102 9 0.5551496694962879 1102 10-11 0.605730214156516 1102 12-13 0.5461646527618953 1102 14-15 0.5534641555458606 1102 16-17 0.43926132243207405 1102 18-19 0.5249961593533499 1102 20-21 0.5742726876425479 1102 22-23 0.6241993562498962 1102 24-25 0.5330234589070173 1102 26-27 0.4771608680498858 1102 28-29 0.48999199736406496 1102 30-31 0.47701745922342553 1102 32-33 0.2882263411667907 1102 34-35 0.1766115739661842 1102 36-37 0.4307779658492734 1102 38-39 0.5599869522152332 1102 40-41 0.4207996076622962 1102 42-43 0.3905877864508298 1102 44-45 0.3714365636468884 1102 46-47 0.5058387916971796 1102 48-49 0.6237368474917631 1102 50-51 0.6545677999514368 1102 52-53 0.4708089181171573 1102 54-55 0.6306785696397057 1102 56-57 0.8173140395559102 1102 58-59 0.9051530524075417 1102 60-61 0.7706932354289364 1102 62-63 0.8841668381996612 1102 64-65 0.819228009499156 1102 66-67 0.6642589796234475 1102 68-69 0.6851430875242599 1102 70-71 0.5510051722990923 1102 72-73 0.6271738398135973 1102 74-75 0.6308625946141397 1102 76-77 0.8307229717664875 1102 78-79 0.5969406022593304 1102 80-81 0.7535522387878757 1102 82-83 0.9270828308608792 1102 84-85 0.7969516665217995 1102 86-87 0.7326513943469131 1102 88-89 0.7259854744107486 1102 90-91 0.6648963688192673 1102 92-93 0.5767444430988853 1102 94-95 0.6572070881829006 1102 96-97 0.39728275509268585 1102 98-99 0.4089726701144514 1102 100-101 0.4444804387698369 1103 1 0.30629699363034035 1103 2 0.33196479463270734 1103 3 0.20214547133651095 1103 4 0.2726278788733083 1103 5 0.4714431519667599 1103 6 0.22900087490629772 1103 7 0.25334692098915923 1103 8 0.2721362497260884 1103 9 0.34208002829277007 1103 10-11 0.3881959440001097 1103 12-13 0.4598326736556899 1103 14-15 0.39877999483214666 1103 16-17 0.5619837612111809 1103 18-19 0.41647490461009795 1103 20-21 0.4895818385754822 1103 22-23 0.47493681014413625 1103 24-25 0.5060652444456153 1103 26-27 0.319910910745115 1103 28-29 0.062096256749690326 1103 30-31 0.14900560797840257 1103 32-33 0.4705666388699612 1103 34-35 0.14879928467822623 1103 36-37 0.20107266163806514 1103 38-39 0.3300725944366363 1103 40-41 0.07613508335527541 1103 42-43 0.18661138007654898 1103 44-45 0.31855672743329677 1103 46-47 0.23842525890621857 1103 48-49 0.35085305133753053 1103 50-51 0.38153720702857186 1103 52-53 0.47121732952096806 1103 54-55 0.4397652045678839 1103 56-57 0.37775212329844976 1103 58-59 0.3015226985779975 1103 60-61 0.36257627565635175 1103 62-63 0.5272674921341931 1103 64-65 0.5565412305978867 1103 66-67 0.3346413792522185 1103 68-69 0.3329568873050306 1103 70-71 0.5584601687984261 1103 72-73 0.6159694871448274 1103 74-75 0.2699432624152607 1103 76-77 0.3437079369196887 1103 78-79 0.3763366977034224 1103 80-81 0.4233590181790383 1103 82-83 0.32959633854500225 1103 84-85 0.40476700401148236 1103 86-87 0.3600587422821313 1103 88-89 0.3789602947675661 1103 90-91 0.4975330837287153 1103 92-93 0.5708159288726975 1103 94-95 0.420240021689235 1103 96-97 0.5393124560083002 1103 98-99 0.6285569920154366 1103 100-101 1.0262142599968875 1104 1 0.229350857614385 1104 2 0.37770266774209205 1104 3 0.3679588072218607 1104 4 0.4375144665681745 1104 5 0.47949045060485673 1104 6 0.3504597749962315 1104 7 0.4200103038100451 1104 8 0.40535929275338134 1104 9 0.5033412502493135 1104 10-11 0.5448776084595579 1104 12-13 0.4070115452043055 1104 14-15 0.4246138922832756 1104 16-17 0.3851082044234033 1104 18-19 0.5108614282699762 1104 20-21 0.6380744126273754 1104 22-23 0.4182609158708388 1104 24-25 0.4103283564899627 1104 26-27 0.13280664348135218 1104 28-29 0.43959168634005863 1104 30-31 0.4482932010930156 1104 32-33 0.05826854260917713 1104 34-35 0.07179826152570712 1104 36-37 0.2017686023824723 1104 38-39 0.4158822993268032 1104 40-41 0.2501948596690795 1104 42-43 0.3974349826711645 1104 44-45 0.40077273224084564 1104 46-47 0.46492265739667715 1104 48-49 0.5201330728420288 1104 50-51 0.2382645404003796 1104 52-53 0.2884670899175106 1104 54-55 0.5207888215175913 1104 56-57 0.48896929480949325 1104 58-59 0.3047638543603597 1104 60-61 0.37529003642443826 1104 62-63 0.6517465920977372 1104 64-65 0.37719751186772044 1104 66-67 0.5064602826635252 1104 68-69 0.5529740852744922 1104 70-71 0.6696210458104233 1104 72-73 0.6405191663365493 1104 74-75 0.4486184839693017 1104 76-77 0.6665991098015063 1104 78-79 0.4431744527350965 1104 80-81 0.4812498181097986 1104 82-83 0.7392507043945074 1104 84-85 0.9089380745498943 1104 86-87 0.894728413198564 1104 88-89 1.0002626257820353 1104 90-91 0.8282414514837875 1104 92-93 0.64557783832581 1104 94-95 0.5717028342468495 1104 96-97 0.6063694077456212 1104 98-99 1.0258012355510715 1104 100-101 1.152515190685694 1105 1 0.46194661965461137 1105 2 0.44663218507431424 1105 3 0.5236140654340957 1105 4 0.4401209566350204 1105 5 0.5231732933917783 1105 6 0.3970535097664012 1105 7 0.44784166981948204 1105 8 0.6786818513488058 1105 9 0.7103750365264645 1105 10-11 0.7686951510408022 1105 12-13 0.5840220381174248 1105 14-15 0.2857521218941841 1105 16-17 0.5146970049241517 1105 18-19 0.714118606627693 1105 20-21 0.5369510839184528 1105 22-23 0.23646056927305636 1105 24-25 0.5012725162550353 1105 26-27 0.6103879483768537 1105 28-29 0.5166489683968436 1105 30-31 0.33928695267925235 1105 32-33 0.4416798837993454 1105 34-35 0.5536221194735589 1105 36-37 0.5168662367669654 1105 38-39 0.46031369056033355 1105 40-41 0.17842607433393454 1105 42-43 0.5441676056814586 1105 44-45 0.2956544203246452 1105 46-47 0.5909629567849208 1105 48-49 0.8361540337555766 1105 50-51 0.83712198220978 1105 52-53 0.6825702564759624 1105 54-55 0.6558416753439431 1105 56-57 0.8909363072954193 1105 58-59 1.0252185732128751 1105 60-61 0.7793030034789652 1105 62-63 0.9801643405200622 1105 64-65 0.9563303323110688 1105 66-67 1.1466233517822246 1105 68-69 1.0220940289718143 1105 70-71 1.006544141897102 1105 72-73 0.9748793695618083 1105 74-75 1.04261444811371 1105 76-77 0.9513865656398686 1105 78-79 1.1843405689870465 1105 80-81 1.0222428690266625 1105 82-83 1.1755010800311432 1105 84-85 1.3355328369684827 1105 86-87 1.3276428240991116 1105 88-89 1.122356988819078 1105 90-91 1.045935533007448 1105 92-93 0.9004973158444436 1105 94-95 0.9089766542806998 1105 96-97 1.3797075367715514 1105 98-99 1.536998321389298 1105 100-101 1.8618245953814245 1106 1 0.16568871153324594 1106 2 0.20954632223445557 1106 3 0.3650624521732908 1106 4 0.435357271812542 1106 5 0.3991552597599579 1106 6 0.38600499745202654 1106 7 0.36725994602500833 1106 8 0.4700122281633554 1106 9 0.6066480751626813 1106 10-11 0.41776956000000354 1106 12-13 0.6377802719089658 1106 14-15 0.6820854398542693 1106 16-17 0.7887618056018653 1106 18-19 0.800271515448955 1106 20-21 0.5258190301250352 1106 22-23 0.41218774424411464 1106 24-25 0.4112156109550611 1106 26-27 0.42521006773315406 1106 28-29 0.5103099299693881 1106 30-31 0.38918265731218327 1106 32-33 0.2510792913628279 1106 34-35 0.2898282682882396 1106 36-37 0.18501561297710367 1106 38-39 0.21592371327228932 1106 40-41 0.11594028447655802 1106 42-43 0.036048506683385995 1106 44-45 0.3831403517777474 1106 46-47 0.27884167478529065 1106 48-49 0.3026561257313105 1106 50-51 0.1574916198784564 1106 52-53 0.43186025504382286 1106 54-55 0.39229157108903934 1106 56-57 0.1695354077175395 1106 58-59 0.15009063135936174 1106 60-61 0.45192680466885804 1106 62-63 0.5454110447783691 1106 64-65 0.3396038717915957 1106 66-67 0.40930264231481317 1106 68-69 0.39999542656209286 1106 70-71 0.4163747691338102 1106 72-73 0.457519328846967 1106 74-75 0.37498553734086926 1106 76-77 0.35209033686001234 1106 78-79 0.44224788123313274 1106 80-81 0.4300721417990978 1106 82-83 0.6926167669767445 1106 84-85 0.4877870814015459 1106 86-87 0.6493638918441711 1106 88-89 0.7761489628496143 1106 90-91 0.9353043639850327 1106 92-93 1.0102263178049355 1106 94-95 0.9120088256390417 1106 96-97 0.9185597860803618 1106 98-99 1.18787261432092 1106 100-101 1.5586064481493764 1107 1 0.18504943660545337 1107 2 0.217636879312181 1107 3 0.060982272114426195 1107 4 0.2776628607276024 1107 5 0.32834364827158424 1107 6 0.2647153450656674 1107 7 0.29867983329585 1107 8 0.2809641602510382 1107 9 0.31976032889398454 1107 10-11 0.3395915292701801 1107 12-13 0.31713286245368266 1107 14-15 0.11704769399779735 1107 16-17 0.3851518342101201 1107 18-19 0.45447852268483757 1107 20-21 0.5162018750660948 1107 22-23 0.63023292934583 1107 24-25 0.5853961809765806 1107 26-27 0.28562459885733915 1107 28-29 0.4799717150035008 1107 30-31 0.5702569645719748 1107 32-33 0.3534809006801609 1107 34-35 0.46589819224689677 1107 36-37 0.3908878624212875 1107 38-39 0.1508749748110887 1107 40-41 0.24360015130115187 1107 42-43 0.4462897668755872 1107 44-45 0.22593098937630174 1107 46-47 0.2910420919546439 1107 48-49 0.4840327296210418 1107 50-51 0.32243565377964956 1107 52-53 0.4221606554494457 1107 54-55 0.3455359009661052 1107 56-57 0.41809468946074446 1107 58-59 0.637233406057689 1107 60-61 0.6221843888790772 1107 62-63 0.6471654644925096 1107 64-65 0.653095095570805 1107 66-67 0.6193768149151921 1107 68-69 0.47018458180790645 1107 70-71 0.613576601601018 1107 72-73 0.646928778639623 1107 74-75 0.5901561563069571 1107 76-77 0.5247745385633173 1107 78-79 0.47091109805401743 1107 80-81 0.460767616418547 1107 82-83 0.5805347951233095 1107 84-85 0.7168427342111166 1107 86-87 0.6809662563534467 1107 88-89 0.5888720783736439 1107 90-91 0.626566067673874 1107 92-93 0.8248552761731958 1107 94-95 0.6885209271227666 1107 96-97 1.01084132867258 1107 98-99 1.052009516220167 1107 100-101 1.550047331295243 1108 1 0.11730865340415875 1108 2 0.0995166709397104 1108 3 0.11605595594418361 1108 4 0.20686505461527815 1108 5 0.15443123031321448 1108 6 0.08263154638277115 1108 7 0.10272166399095539 1108 8 0.16430114513116933 1108 9 0.14736594046504337 1108 10-11 -0.017575785700188362 1108 12-13 0.1330854496191094 1108 14-15 0.2633190673644137 1108 16-17 0.2815205250668811 1108 18-19 0.14689441755974997 1108 20-21 0.09956730501620825 1108 22-23 0.2944362063658801 1108 24-25 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-2.1291032625642643 2316 66-67 -2.395803629690942 2316 68-69 -2.6287225507028893 2316 70-71 -2.7771735789259893 2316 72-73 -2.2170847869809585 2316 74-75 -1.769721616377339 2316 76-77 -1.67381707607149 2316 78-79 -2.718537273662541 2316 80-81 -2.2268931869098623 2316 82-83 -2.1713295315796124 2316 84-85 -1.9897995000143638 2316 86-87 -3.0993792201554555 2316 88-89 -3.594050463178089 2316 90-91 -3.3156799386047417 2316 92-93 -3.672067066386802 2316 94-95 -3.825758866243241 2316 96-97 -4.507210456789913 2316 98-99 -5.867902414677207 2316 100-101 -6.163123479913487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3464.0 3 1277.0 4 1340.0 5 1460.0 6 1509.0 7 1779.0 8 2270.0 9 2845.0 10 3590.0 11 4591.0 12 5699.0 13 6945.0 14 8114.0 15 9421.0 16 11757.0 17 14338.0 18 12308.0 19 8999.0 20 9076.0 21 9750.0 22 10775.0 23 11974.0 24 13848.0 25 16115.0 26 18463.0 27 22074.0 28 25842.0 29 31096.0 30 38364.0 31 46798.0 32 59209.0 33 76169.0 34 101002.0 35 139080.0 36 197360.0 37 264351.0 38 215551.0 39 33063.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38733241651876 18.356922111930015 14.601081582157363 22.654663889393863 2 15.309819364542834 19.654686281529337 40.83779441026367 24.197699943664162 3 19.78903609308831 24.0450844466152 29.83909908809866 26.326780372197835 4 12.55020156966871 16.12425491397635 37.778784835742755 33.54675868061218 5 13.552380919333626 36.98459680693439 34.49947429967348 14.96354797405851 6 31.661568397764906 35.61413784201658 17.79020577205464 14.934087988163874 7 28.373213046495486 32.58584316446912 20.732685634975713 18.308258154059683 8 26.172092122766514 34.12232656547845 19.939184373909235 19.766396937845794 9 26.0310151106374 17.09876594610798 20.98800641730229 35.882212525952326 10-11 24.9572857172599 26.306203460169193 27.32055490398829 21.415955918582622 12-13 26.20084624178783 23.747751248631666 27.791530989832257 22.259871519748245 14-15 22.810748135334244 25.153784183804106 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21.952782047938022 40-41 22.037839163266927 26.36078894076216 29.693660998526344 21.90771089744457 42-43 21.795474290787826 26.413230650243385 29.24935100518329 22.5419440537855 44-45 22.008127484346947 26.364589500305723 29.195925644998116 22.431357370349215 46-47 22.312915402511248 26.67156375158323 28.810882349268972 22.20463849663655 48-49 22.321493600134843 26.662324483186524 28.688445560741666 22.327736355936963 50-51 21.918123681871325 27.00599199056375 28.855681362193753 22.220202965371172 52-53 22.313935791946037 27.143759893342654 28.238149145478786 22.30415516923252 54-55 21.921971708371125 26.82866343991107 28.520993276532725 22.728371575185083 56-57 22.32726556286176 27.018904679498718 28.15160372911375 22.502226028525772 58-59 22.652072059197927 26.785993006076154 28.185991757408935 22.37594317731699 60-61 22.277186855937394 26.8129137522346 28.863473815722568 22.046425576105438 62-63 22.017119107828044 26.902748359381256 28.802401211704588 22.277731321086108 64-65 21.864900024658724 27.15948505927631 28.533888056334256 22.44172685973071 66-67 21.91364661748017 27.13771202313005 28.136575776157958 22.812065583231824 68-69 21.890091318847286 26.964798225099607 28.52366360420498 22.621446851848127 70-71 22.21718534200802 26.891818680636437 28.21945089758593 22.671545079769615 72-73 22.039394050799125 26.71313692262765 28.654295562203853 22.59317346436937 74-75 21.85668191912532 27.521988200125485 28.11603984529963 22.50529003544957 76-77 22.063437559613615 27.076218719109306 28.06642040823405 22.793923313043027 78-79 22.081909842291644 27.07266654411637 28.191296385575598 22.654127228016385 80-81 22.22952163885855 27.251632049742092 27.951867322966695 22.56697898843266 82-83 22.191364993612936 27.107375871913252 28.264608654845652 22.43665047962816 84-85 22.006175687272407 27.178435182686435 28.11595751249255 22.69943161754861 86-87 22.049657989166363 27.23205336215274 28.091051670307245 22.62723697837365 88-89 21.977956422000847 27.175414244876695 27.781928261603955 23.0647010715185 90-91 21.979005679689905 26.989764349749034 28.058001093208514 22.973228877352547 92-93 22.223937334648227 27.037510202510067 28.15778309732423 22.580769365517476 94-95 21.868627711458092 26.89029024077994 28.327290624743114 22.913791423018854 96-97 21.542285951530594 26.950706133022926 28.618079794054797 22.888928121391682 98-99 21.61996404119271 27.173251309603387 27.96801518553683 23.23876946366707 100-101 21.487173206554377 26.992211393244325 28.24503597316992 23.27557942703138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 135.0 1 109.5 2 150.5 3 233.0 4 206.0 5 198.5 6 336.5 7 680.0 8 1173.0 9 1519.5 10 1437.0 11 1273.0 12 1299.5 13 1422.0 14 1592.5 15 1716.5 16 1818.0 17 1910.5 18 2049.0 19 2255.5 20 2554.0 21 2964.0 22 3476.0 23 4213.0 24 5165.5 25 6283.0 26 7962.5 27 10046.0 28 12031.0 29 14355.5 30 17182.0 31 20245.5 32 23846.5 33 26620.5 34 29119.5 35 32015.0 36 34947.5 37 37845.0 38 39758.5 39 41110.5 40 42367.0 41 44556.5 42 46630.0 43 48563.5 44 51133.5 45 53124.0 46 54121.5 47 57761.0 48 65957.5 49 65020.5 50 56147.0 51 51303.5 52 48182.5 53 46658.0 54 45171.5 55 41525.5 56 37698.0 57 33720.5 58 30121.5 59 26551.0 60 22208.5 61 18237.5 62 14885.0 63 11563.5 64 8522.5 65 6539.5 66 4951.0 67 3847.5 68 2913.0 69 2039.0 70 1517.5 71 1163.5 72 912.0 73 759.5 74 605.5 75 473.5 76 329.0 77 210.5 78 156.5 79 105.5 80 70.5 81 55.5 82 33.0 83 22.0 84 17.0 85 9.5 86 4.5 87 3.0 88 4.0 89 4.0 90 4.5 91 4.0 92 2.5 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03183783375101704 2 0.02254312847294235 3 0.020600951250658084 4 0.051328969446084116 5 0.051745150279430746 6 0.04640416291814902 7 0.047236524584842274 8 0.04189553722356055 9 0.04002272347350073 10-11 0.04931742875157541 12-13 0.041860855487448334 14-15 0.03731754805674765 16-17 0.045224983890333574 18-19 0.05042724430716643 20-21 0.010994110347573423 22-23 0.018762819236710478 24-25 0.005583759514067259 26-27 0.010716656458675672 28-29 0.006381439444648296 30-31 0.008150207986371465 32-33 0.0034681736112219 34-35 0.009155978333625817 36-37 0.008635752291942531 38-39 0.010612611250339014 40-41 0.001838132013947607 42-43 0.001976858958396483 44-45 0.002046222430620921 46-47 4.85544305571066E-4 48-49 6.9363472224438E-4 50-51 4.50862569458847E-4 52-53 0.00381499097234409 54-55 0.006138667291862763 56-57 0.014739737847693075 58-59 0.009988340000319071 60-61 0.010265793889216824 62-63 0.005236942152945069 64-65 3.4681736112219E-5 66-67 0.006450802916872734 68-69 0.017202141111660622 70-71 0.006381439444648296 72-73 0.002913265833426396 74-75 0.003849672708456309 76-77 0.007352528055790428 78-79 0.0066588933335460475 80-81 0.011479654653144489 82-83 0.0066242115974338295 84-85 0.015398690833825236 86-87 0.00416180833346628 88-89 0.005687804722403916 90-91 0.003676264027895214 92-93 0.01709809590332397 94-95 0.009468113958635786 96-97 0.009190660069738035 98-99 0.02146799465346356 100-101 0.037282866320635426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1441681.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.51997755608025 #Duplication Level Percentage of deduplicated Percentage of total 1 83.46753724258821 55.52258704038261 2 10.388387712537298 13.820706349636819 3 2.7912292508403125 5.570175213593169 4 1.1217430721383597 2.984732959293288 5 0.5912952266224627 1.9666472601971803 6 0.345436841530343 1.3787070567385 7 0.23405673561489523 1.0898614159966569 8 0.16823834005051447 0.8952968483385936 9 0.12426679860141396 0.7439602188538802 >10 0.6953915187732685 8.483457899724927 >50 0.04735466002025081 2.128466890168511 >100 0.022765261274255853 2.8232438698320936 >500 0.001044245185634271 0.4865894800116131 >1k 0.0011486697041976981 1.7270627040012472 >5k 1.044245185634271E-4 0.3785047932310178 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5449 0.37796156015096266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4386 0.30422818917638506 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4208 0.2918814911204351 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3321 0.23035609125735856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2583 0.17916584875572336 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2206 0.15301581972711023 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2099 0.14559392819909536 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.38726944448876E-4 0.0 0.0 0.0 6.9363472224438E-5 7 1.38726944448876E-4 0.0 0.0 0.0 6.9363472224438E-5 8 1.38726944448876E-4 0.0 0.0 0.0 6.9363472224438E-5 9 1.38726944448876E-4 0.0 0.0 0.0 6.9363472224438E-5 10-11 1.38726944448876E-4 0.0 0.0 0.0 6.9363472224438E-5 12-13 1.38726944448876E-4 0.0 0.0 0.0 2.08090416673314E-4 14-15 1.38726944448876E-4 3.4681736112219E-5 0.0 0.0 2.08090416673314E-4 16-17 1.38726944448876E-4 6.9363472224438E-5 0.0 0.0 2.08090416673314E-4 18-19 1.38726944448876E-4 6.9363472224438E-5 0.0 3.4681736112219E-5 2.08090416673314E-4 20-21 1.38726944448876E-4 6.9363472224438E-5 0.0 2.08090416673314E-4 2.08090416673314E-4 22-23 1.38726944448876E-4 6.9363472224438E-5 0.0 5.20226041683285E-4 2.08090416673314E-4 24-25 2.08090416673314E-4 6.9363472224438E-5 0.0 6.9363472224438E-4 2.08090416673314E-4 26-27 2.08090416673314E-4 6.9363472224438E-5 0.0 9.710886111421321E-4 2.77453888897752E-4 28-29 2.77453888897752E-4 6.9363472224438E-5 0.0 0.001768768541723169 2.77453888897752E-4 30-31 3.4681736112219E-4 6.9363472224438E-5 0.0 0.0035722188195585567 2.77453888897752E-4 32-33 3.4681736112219E-4 6.9363472224438E-5 0.0 0.0052369421529450694 3.4681736112219E-4 34-35 3.4681736112219E-4 6.9363472224438E-5 0.0 0.007109755903004895 3.4681736112219E-4 36-37 3.4681736112219E-4 6.9363472224438E-5 0.0 0.009849613055870196 3.4681736112219E-4 38-39 4.16180833346628E-4 6.9363472224438E-5 0.0 0.014462283958795324 3.4681736112219E-4 40-41 4.16180833346628E-4 6.9363472224438E-5 0.0 0.02001136173675036 3.4681736112219E-4 42-43 5.20226041683285E-4 6.9363472224438E-5 0.0 0.026288755973062003 3.4681736112219E-4 44-45 5.54907777795504E-4 6.9363472224438E-5 0.0 0.03180315201490482 3.4681736112219E-4 46-47 5.54907777795504E-4 6.9363472224438E-5 0.0 0.044184531806967006 3.4681736112219E-4 48-49 5.54907777795504E-4 6.9363472224438E-5 0.0 0.06117858250195432 3.4681736112219E-4 50-51 5.54907777795504E-4 6.9363472224438E-5 0.0 0.06825365666884699 3.4681736112219E-4 52-53 5.54907777795504E-4 6.9363472224438E-5 0.0 0.07938649396086929 4.85544305571066E-4 54-55 5.54907777795504E-4 6.9363472224438E-5 0.0 0.09797590451701868 4.85544305571066E-4 56-57 5.54907777795504E-4 6.9363472224438E-5 0.0 0.1270391993790582 5.20226041683285E-4 58-59 5.54907777795504E-4 6.9363472224438E-5 0.0 0.16331629535243927 5.54907777795504E-4 60-61 5.54907777795504E-4 6.9363472224438E-5 0.0 0.2044835161176432 5.54907777795504E-4 62-63 5.54907777795504E-4 6.9363472224438E-5 0.0 0.2481131401468147 5.54907777795504E-4 64-65 5.54907777795504E-4 6.9363472224438E-5 0.0 0.29736120542616573 5.54907777795504E-4 66-67 5.54907777795504E-4 6.9363472224438E-5 0.0 0.3652680447338905 5.54907777795504E-4 68-69 5.54907777795504E-4 6.9363472224438E-5 0.0 0.4519377032783258 5.54907777795504E-4 70-71 5.54907777795504E-4 6.9363472224438E-5 0.0 0.5001453164743102 5.54907777795504E-4 72-73 5.89589513907723E-4 6.9363472224438E-5 0.0 0.5620868971707333 6.242712500199421E-4 74-75 6.24271250019942E-4 6.9363472224438E-5 0.0 0.6282943314089594 7.28316458356599E-4 76-77 6.24271250019942E-4 6.9363472224438E-5 0.0 0.7005710694668238 7.62998194468818E-4 78-79 6.24271250019942E-4 6.9363472224438E-5 0.0 0.7897378130113388 7.62998194468818E-4 80-81 6.24271250019942E-4 6.9363472224438E-5 0.0 0.9204532764182922 7.976799305810371E-4 82-83 6.24271250019942E-4 6.9363472224438E-5 0.0 1.0562669550337418 9.01725138917694E-4 84-85 6.589529861321609E-4 6.9363472224438E-5 0.0 1.2694209051794398 9.01725138917694E-4 86-87 7.28316458356599E-4 6.9363472224438E-5 0.0 1.5665393384528201 9.01725138917694E-4 88-89 7.62998194468818E-4 6.9363472224438E-5 0.0 1.8999001859634692 9.01725138917694E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6280 0.0 26.407043 1 TACATGG 6365 0.0 25.458044 2 GTGTAGC 880 0.0 24.838715 1 ACATGGG 6320 0.0 24.361105 3 GTATCAA 10755 0.0 23.769817 1 TACCTGG 1245 0.0 23.282495 2 GAGTACT 5565 0.0 22.715155 12-13 GTACCTG 1400 0.0 21.043484 1 GGTATCA 8185 0.0 20.957647 1 GTACTTT 6060 0.0 20.702147 14-15 TCAACGC 12320 0.0 20.67537 4 ATCAACG 12320 0.0 20.635363 3 CAACGCA 12370 0.0 20.629501 5 TATCAAC 12355 0.0 20.615368 2 AACGCAG 12560 0.0 20.505182 6 CATGGGG 3025 0.0 19.951591 4 AGTACTT 5820 0.0 19.719383 12-13 AGAGTAC 9280 0.0 19.227863 10-11 TACACCG 225 1.661243E-6 19.008337 5 ACACCGT 255 2.6656198E-7 18.634333 6 >>END_MODULE