FastQCFastQC Report
Thu 26 May 2016
SRR936669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441681
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65170.45204174848666245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56750.3936377048736856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50290.3488289018166987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30870.21412503875684008No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23420.1624492519496338No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18850.13075014514306563No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.10924746875348985No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15480.10737465500343002No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14450.1002302173643129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG64050.023.4377861
GAGTACT61800.022.82739412-13
TACATGG64650.022.4799882
AGTACTT64300.021.3119512-13
ACATGGG64500.021.205373
GTACTTT67400.021.1421814-15
CGTATAC1150.003727733620.6483463
GTAAGGT5150.020.2874984
GTGTAGC7050.020.2153261
TACCTGG11850.020.0398672
CGGTTAC1603.965397E-1019.29630728-29
GTATCAA143800.019.2270531
AGAGTAC105500.018.93289210-11
TAAGGTG6700.018.4300565
GTACACG2352.5252648E-618.1937941
GTACCTG13150.018.0631261
ACTTTTT79750.017.98599616-17
TACTTTT76850.017.86250514-15
TACCGCT1350.00940969617.589947
CATGGGG28500.017.4967544
Produced by FastQC (version 0.11.5)