##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936669_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1441681 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95236532908459 31.0 31.0 34.0 30.0 34.0 2 32.099580975264296 33.0 31.0 34.0 30.0 34.0 3 32.20722129236634 33.0 31.0 34.0 30.0 34.0 4 35.92912440408107 37.0 35.0 37.0 35.0 37.0 5 35.71982914389522 37.0 35.0 37.0 33.0 37.0 6 35.82608496609166 37.0 35.0 37.0 35.0 37.0 7 35.77628892938174 37.0 35.0 37.0 33.0 37.0 8 35.751832756344854 37.0 35.0 37.0 33.0 37.0 9 37.39823719671689 39.0 37.0 39.0 34.0 39.0 10-11 37.27204111034272 39.0 37.0 39.0 34.0 39.0 12-13 37.18377123649407 39.0 37.0 39.0 33.0 39.0 14-15 38.30315999170413 40.0 38.0 41.0 33.0 41.0 16-17 38.23832075195553 40.0 38.0 41.0 33.0 41.0 18-19 38.38487987287063 40.0 38.0 41.0 33.5 41.0 20-21 38.4215076011961 40.0 38.0 41.0 34.0 41.0 22-23 38.335311695166965 40.0 38.0 41.0 34.0 41.0 24-25 38.21159500610746 40.0 38.0 41.0 33.5 41.0 26-27 38.00394782202166 40.0 37.0 41.0 33.0 41.0 28-29 37.93299003038814 40.0 37.0 41.0 33.0 41.0 30-31 37.91236688282637 40.0 37.0 41.0 33.0 41.0 32-33 37.77936832073115 40.0 37.0 41.0 33.0 41.0 34-35 37.60869152052361 40.0 37.0 41.0 33.0 41.0 36-37 37.357759102048234 40.0 36.0 41.0 32.5 41.0 38-39 37.19929131340429 39.0 36.0 41.0 31.0 41.0 40-41 36.99787921183674 39.0 36.0 41.0 31.0 41.0 42-43 36.75840390488603 39.0 35.5 41.0 31.0 41.0 44-45 36.508790432835 39.0 35.0 40.5 30.0 41.0 46-47 36.15515290830635 39.0 35.0 40.0 30.0 41.0 48-49 35.795678794407365 39.0 35.0 40.0 28.5 41.0 50-51 35.47978436283755 38.5 35.0 40.0 27.5 41.0 52-53 35.21047513284839 38.0 34.5 40.0 27.0 41.0 54-55 34.956580547291665 38.0 34.0 40.0 26.5 41.0 56-57 34.653465295027125 38.0 34.0 40.0 26.0 41.0 58-59 34.31720782891638 38.0 33.5 40.0 25.5 41.0 60-61 33.951188577778304 37.0 33.0 40.0 24.5 41.0 62-63 33.56178794060544 36.5 33.0 39.0 23.0 40.5 64-65 33.15355893571463 36.0 32.0 39.0 22.5 40.0 66-67 32.695352855451375 35.5 31.5 39.0 22.0 40.0 68-69 32.40606070274909 35.0 31.0 38.0 21.5 40.0 70-71 31.95702169897502 35.0 31.0 38.0 20.0 40.0 72-73 31.50296355435079 35.0 31.0 37.0 18.5 39.0 74-75 30.981253827996625 34.0 30.0 36.5 17.5 39.0 76-77 29.567291585309093 33.0 28.5 35.0 14.5 37.5 78-79 30.34919167277643 34.0 30.0 36.0 16.0 37.5 80-81 30.128986925679122 34.0 30.0 35.0 11.5 37.0 82-83 29.78593426701191 34.0 29.5 35.0 7.5 37.0 84-85 29.368110213008286 34.0 29.0 35.0 7.0 36.0 86-87 28.996880377836707 34.0 29.0 35.0 2.0 36.0 88-89 28.584034887051992 33.5 29.0 35.0 2.0 35.5 90-91 28.10132720067754 33.0 27.5 35.0 2.0 35.0 92-93 27.614557936186994 33.0 27.0 35.0 2.0 35.0 94-95 27.02357595057436 33.0 25.5 34.0 2.0 35.0 96-97 26.24565836686479 32.0 25.0 34.0 2.0 35.0 98-99 25.301385674084628 32.0 22.0 34.0 2.0 35.0 100-101 22.024874087957045 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3094131630646473 1101 2 0.32262341166377695 1101 3 0.3746144755644707 1101 4 0.23897418032832718 1101 5 0.18135335335620084 1101 6 0.19477195545303516 1101 7 0.18743937944157807 1101 8 0.18738568500290143 1101 9 0.2632688961858989 1101 10-11 0.3730156749660054 1101 12-13 0.270357994103712 1101 14-15 0.3669788714610007 1101 16-17 0.359662613211043 1101 18-19 0.3731883408543055 1101 20-21 0.34513472983270077 1101 22-23 0.3730165553670517 1101 24-25 0.2691857049119548 1101 26-27 0.16460989175536866 1101 28-29 0.16174264434339847 1101 30-31 0.2936583784768487 1101 32-33 0.3035651055870119 1101 34-35 0.21538900290996565 1101 36-37 0.27829709120759816 1101 38-39 0.36194499453144857 1101 40-41 0.34310236247015524 1101 42-43 0.341040507058338 1101 44-45 0.323539263442278 1101 46-47 0.2162583779578071 1101 48-49 0.28543840192801184 1101 50-51 0.21898367232982707 1101 52-53 0.17137810724570102 1101 54-55 0.17588861587517357 1101 56-57 0.3248387881819568 1101 58-59 0.3810040521869382 1101 60-61 0.13852604681186875 1101 62-63 0.30628832629755465 1101 64-65 0.45056925723746843 1101 66-67 0.24283092197295986 1101 68-69 0.15990213447332735 1101 70-71 0.20004350691202433 1101 72-73 0.18696853314311213 1101 74-75 0.29875169455684514 1101 76-77 0.13304642802895827 1101 78-79 0.10277925091973827 1101 80-81 0.08895721443166593 1101 82-83 0.20332826842352603 1101 84-85 0.28754544461005693 1101 86-87 0.24753136452512692 1101 88-89 0.07682673161312792 1101 90-91 0.26443364948062964 1101 92-93 0.2788129212209469 1101 94-95 0.20787904873123253 1101 96-97 0.2868837842073937 1101 98-99 0.4796098481455502 1101 100-101 0.8759278240256698 1102 1 0.23854514162907492 1102 2 0.23757155982195854 1102 3 0.27418145698340624 1102 4 0.17381111436173313 1102 5 0.07580151878365626 1102 6 0.210469725549558 1102 7 0.23773021433309793 1102 8 0.33236124051481397 1102 9 0.29307769484715607 1102 10-11 0.3608244140313417 1102 12-13 0.327649995856369 1102 14-15 0.49579478844929525 1102 16-17 0.46095841826734585 1102 18-19 0.3407109314350336 1102 20-21 0.1225270152755158 1102 22-23 0.13766716409202928 1102 24-25 0.1936742804406535 1102 26-27 0.11604107437556621 1102 28-29 0.13590560940325958 1102 30-31 0.2693056545705588 1102 32-33 0.28389370939667913 1102 34-35 0.30677224467098796 1102 36-37 0.39185780557312455 1102 38-39 0.3368970976920025 1102 40-41 0.32726322135579977 1102 42-43 0.4895811518185269 1102 44-45 0.3701385370492005 1102 46-47 0.3079736299774609 1102 48-49 0.13951235911782334 1102 50-51 0.041605540919455564 1102 52-53 0.12474277133123479 1102 54-55 0.12977694529978123 1102 56-57 0.2899800813187454 1102 58-59 -0.13290939556605252 1102 60-61 0.1274461144532495 1102 62-63 0.03579122177916361 1102 64-65 0.12690733815384192 1102 66-67 0.059906813998686914 1102 68-69 -0.05937989037951752 1102 70-71 -0.11756485439640585 1102 72-73 -0.0773695355831805 1102 74-75 -0.1843300040083129 1102 76-77 -0.2689323260233927 1102 78-79 -0.16462106642856966 1102 80-81 0.2293555657916393 1102 82-83 0.10492371231497799 1102 84-85 0.07938347778196331 1102 86-87 -0.19354790415158973 1102 88-89 -0.29877879347100134 1102 90-91 -0.10437895587040913 1102 92-93 -0.07410619559451703 1102 94-95 -0.09978593547922898 1102 96-97 -0.06495549506114529 1102 98-99 0.23030603691835694 1102 100-101 0.6530571763059889 1103 1 0.10476582735199003 1103 2 0.12986287537275132 1103 3 0.10995176409653595 1103 4 0.043859402503890976 1103 5 -0.12580266541754526 1103 6 0.07575553868798579 1103 7 0.010077853575701567 1103 8 -0.0035315389893000315 1103 9 -0.04797797633897005 1103 10-11 -0.011571771906240258 1103 12-13 0.14366703720916973 1103 14-15 0.077080564846014 1103 16-17 0.2628955572714702 1103 18-19 -0.1260842518619043 1103 20-21 -0.004616166516377973 1103 22-23 -0.018343610756765827 1103 24-25 0.09694065312822175 1103 26-27 0.11774735292268446 1103 28-29 0.12127356069189688 1103 30-31 0.03386796205926146 1103 32-33 0.18008917261107626 1103 34-35 0.11208519905206771 1103 36-37 0.10405449078982798 1103 38-39 0.23160503165761526 1103 40-41 0.31348967008511863 1103 42-43 0.26366755881197435 1103 44-45 0.27600608724076636 1103 46-47 0.17355269645207727 1103 48-49 0.1625677867199684 1103 50-51 0.17147033139981005 1103 52-53 0.10630200136092327 1103 54-55 -0.027602118317140878 1103 56-57 0.06083542849735579 1103 58-59 0.3061559972063037 1103 60-61 0.14997099846168993 1103 62-63 0.1563620587895045 1103 64-65 -0.1636774607090743 1103 66-67 0.26203933048652317 1103 68-69 0.25034002887034745 1103 70-71 0.14764111354712384 1103 72-73 0.1806923796536779 1103 74-75 0.14112489587391153 1103 76-77 0.011376188733510872 1103 78-79 0.029693799202210158 1103 80-81 0.14957654000507503 1103 82-83 0.24647066788947924 1103 84-85 0.09999200907263983 1103 86-87 0.027871122206509114 1103 88-89 -0.044194073534743694 1103 90-91 -0.23079801965062785 1103 92-93 -0.1184687574175598 1103 94-95 0.1975790710288159 1103 96-97 0.09535406071107744 1103 98-99 0.2904671969768451 1103 100-101 0.7980685696403604 1104 1 0.13728223368006098 1104 2 0.1845100566368174 1104 3 0.1092558233521288 1104 4 0.059985665435789315 1104 5 -0.023357936060385498 1104 6 0.031955603708134106 1104 7 0.14573915209276578 1104 8 0.023983101098856707 1104 9 0.09164645491066636 1104 10-11 0.26579052119388535 1104 12-13 0.16002868151519323 1104 14-15 0.1710610644315409 1104 16-17 0.2414107617119825 1104 18-19 0.1837800221008976 1104 20-21 0.11221145550546652 1104 22-23 0.12754076878637477 1104 24-25 0.11516388614944617 1104 26-27 0.16599947909555368 1104 28-29 0.2384283944894321 1104 30-31 0.03902770473710859 1104 32-33 0.22979580790294563 1104 34-35 0.07888375903878142 1104 36-37 0.21134195442709824 1104 38-39 0.1622692082309385 1104 40-41 0.07142437972512283 1104 42-43 0.24951019598407953 1104 44-45 0.13983602718861476 1104 46-47 0.19847004909066612 1104 48-49 0.07433178892108572 1104 50-51 -0.10265291696166656 1104 52-53 -0.033075085933553794 1104 54-55 -0.03895601509273661 1104 56-57 0.018813344165380386 1104 58-59 -0.16500116436876056 1104 60-61 0.015267058268968015 1104 62-63 -0.005923723049605201 1104 64-65 0.10637393616855206 1104 66-67 -0.21958368214173873 1104 68-69 -0.06985639671043486 1104 70-71 -0.08290421126509528 1104 72-73 -0.14150848193363075 1104 74-75 -0.14450635545428625 1104 76-77 -0.19583506622568692 1104 78-79 -0.3891791427060909 1104 80-81 -0.03559295569472809 1104 82-83 0.034724187351191205 1104 84-85 0.10099264379311634 1104 86-87 -0.059756895002717414 1104 88-89 -0.19224227288482254 1104 90-91 0.04433078919999289 1104 92-93 0.10289739748519722 1104 94-95 0.1466962325475869 1104 96-97 0.43664473876424736 1104 98-99 0.5068822506888324 1104 100-101 1.120245154224957 1105 1 0.16430315878161394 1105 2 0.2673291765026633 1105 3 0.21925546523222295 1105 4 0.10498265255748862 1105 5 0.0810449145740364 1105 6 0.089776397395255 1105 7 0.03734637864096868 1105 8 0.13670289718849915 1105 9 0.09126887197087541 1105 10-11 0.19611362319732706 1105 12-13 0.12142958428875517 1105 14-15 0.27623309433299426 1105 16-17 0.34437801865366424 1105 18-19 0.21069195418890985 1105 20-21 0.275897941773124 1105 22-23 0.2195149125294762 1105 24-25 0.1874566164711453 1105 26-27 0.2551604353068271 1105 28-29 0.2952604658393696 1105 30-31 0.4800379609796863 1105 32-33 0.46635774785401907 1105 34-35 0.5248376269672761 1105 36-37 0.46568727232079254 1105 38-39 0.5386703523461662 1105 40-41 0.46686115365648817 1105 42-43 0.500435858077708 1105 44-45 0.6213866240810617 1105 46-47 0.6581184999106853 1105 48-49 0.5479083790902237 1105 50-51 0.7682756100911803 1105 52-53 0.76091994398503 1105 54-55 0.7826741934109052 1105 56-57 0.650468340794383 1105 58-59 0.6560677301151046 1105 60-61 0.7191287585911184 1105 62-63 0.6315830306246255 1105 64-65 0.7040028710109709 1105 66-67 1.0137746417325602 1105 68-69 0.9628668093034847 1105 70-71 0.7390724393173116 1105 72-73 0.8333997693046094 1105 74-75 0.48513733081399124 1105 76-77 0.46492534688897535 1105 78-79 0.5971363951822148 1105 80-81 0.6625345443325195 1105 82-83 0.7960131387294069 1105 84-85 0.672582758438196 1105 86-87 0.5833497640032981 1105 88-89 0.7670523399019267 1105 90-91 0.8861247052099763 1105 92-93 0.8614039129318449 1105 94-95 0.856846051229919 1105 96-97 0.911158279589138 1105 98-99 1.232649211169523 1105 100-101 1.2682432639746963 1106 1 0.08481495856040056 1106 2 0.10659941639818982 1106 3 0.09653283022474568 1106 4 0.03668901052928675 1106 5 -0.02699869367346963 1106 6 -0.03266587807235055 1106 7 0.07263567489402334 1106 8 0.059638411257857626 1106 9 0.10765635231941673 1106 10-11 0.2070154827942403 1106 12-13 0.09518178205672001 1106 14-15 0.2967681158110409 1106 16-17 0.2147865749193656 1106 18-19 0.08788314806744069 1106 20-21 0.04277756841351277 1106 22-23 0.04649525014223599 1106 24-25 0.051870009646883375 1106 26-27 0.035229703936579426 1106 28-29 0.088990890230086 1106 30-31 0.21474602700051548 1106 32-33 0.10322640284834961 1106 34-35 0.24465224984121647 1106 36-37 0.18241247870668076 1106 38-39 0.36689996883212217 1106 40-41 0.4026284770845976 1106 42-43 0.4673423069944249 1106 44-45 0.25860886383419057 1106 46-47 0.23571376753874773 1106 48-49 0.2528809378382917 1106 50-51 0.3013361895979827 1106 52-53 0.24988700720064116 1106 54-55 0.3934317871897477 1106 56-57 0.379481628946543 1106 58-59 0.28909226339535365 1106 60-61 0.1895463769833512 1106 62-63 0.4203371743224409 1106 64-65 0.49096663787805994 1106 66-67 0.4033829717687425 1106 68-69 0.5067410544971196 1106 70-71 0.5651611284078939 1106 72-73 0.24837760350491322 1106 74-75 0.22668710009386928 1106 76-77 0.3157346271538479 1106 78-79 0.4745899457153051 1106 80-81 0.5653482941676664 1106 82-83 0.4519015386410494 1106 84-85 0.3601246808874272 1106 86-87 0.4433609460201531 1106 88-89 0.463233017668081 1106 90-91 0.48218628074158687 1106 92-93 0.37362402874905953 1106 94-95 0.4189313274589104 1106 96-97 0.5358141008322157 1106 98-99 0.8428594363229109 1106 100-101 0.9976218691030496 1107 1 0.09307864916254971 1107 2 0.12515768132545446 1107 3 0.060004404723002835 1107 4 0.08020873263623685 1107 5 0.04872111582469074 1107 6 0.1880656248090844 1107 7 0.0829439899750497 1107 8 -0.04673152836526384 1107 9 0.1218752865314201 1107 10-11 0.06619746811362148 1107 12-13 0.01930807609240759 1107 14-15 0.1426779050003688 1107 16-17 0.11547800975657907 1107 18-19 0.16217666710251422 1107 20-21 0.12771192882254923 1107 22-23 0.08851722985538402 1107 24-25 0.1468007699778653 1107 26-27 0.17275680757760625 1107 28-29 -0.06281148472216813 1107 30-31 0.0074873352853686015 1107 32-33 0.029159606285730888 1107 34-35 -0.0971531755588444 1107 36-37 0.06533024577888114 1107 38-39 0.15375611136223455 1107 40-41 0.04685419923001888 1107 42-43 -0.20940641030601626 1107 44-45 -0.15057573222880904 1107 46-47 -0.031813253657738016 1107 48-49 -0.1333909477506765 1107 50-51 -0.28854621701296423 1107 52-53 -0.3339302297532001 1107 54-55 -0.3035323302002766 1107 56-57 -0.42600423044155633 1107 58-59 -0.33519699600537933 1107 60-61 -0.2987272619260679 1107 62-63 -0.3198347500154597 1107 64-65 -0.20909189774210546 1107 66-67 -0.07901511991507704 1107 68-69 -0.07264341460129486 1107 70-71 -0.19252338933279844 1107 72-73 0.04549671662156385 1107 74-75 -0.1407475832229288 1107 76-77 -0.23048240533754338 1107 78-79 -0.0880225567236792 1107 80-81 0.11736117750040265 1107 82-83 0.16416186550615208 1107 84-85 0.11646535270046954 1107 86-87 0.2210959747795549 1107 88-89 0.0820039894967799 1107 90-91 0.055917802860079746 1107 92-93 0.052426989057511975 1107 94-95 0.22758717923462868 1107 96-97 0.29858819456066144 1107 98-99 0.45222073850686684 1107 100-101 0.6916935573747658 1108 1 0.013406556694697258 1108 2 -0.01828562728012173 1108 3 -0.022117169999361863 1108 4 -0.11311950086325595 1108 5 -0.19229905921114465 1108 6 -0.05928481146511899 1108 7 -0.0021355431805147873 1108 8 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-1.229801366086953 2316 92-93 -1.2001295358875694 2316 94-95 -1.4037253909999912 2316 96-97 -1.4466941448008193 2316 98-99 -1.3733410316405852 2316 100-101 -2.1016361298114496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 17.0 5 36.0 6 94.0 7 207.0 8 520.0 9 964.0 10 1615.0 11 2604.0 12 3529.0 13 5193.0 14 6818.0 15 9010.0 16 11714.0 17 14826.0 18 13340.0 19 8876.0 20 8128.0 21 8408.0 22 9108.0 23 10161.0 24 11905.0 25 14207.0 26 17518.0 27 21601.0 28 26721.0 29 33321.0 30 41115.0 31 51886.0 32 65186.0 33 82883.0 34 110885.0 35 151804.0 36 218188.0 37 289972.0 38 178993.0 39 10322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2195739348197 19.55034240230484 15.047521373689321 22.18256228918614 2 15.750628118917373 20.981460953963843 39.306440178523125 23.961470748595666 3 20.291338910392476 24.493000929509854 29.716499493625225 25.49916066647244 4 13.222582144318743 17.06118598569714 36.859614197423824 32.8566176725603 5 13.595918061578672 37.82091299799954 33.870488905906214 14.712680034515573 6 31.919468673591485 36.47874867775331 16.884249223992924 14.717533424662285 7 28.641842404803835 32.956042286747206 20.193163397450615 18.20895191099834 8 26.592914798766166 34.70219833652521 19.19155485852973 19.5133320061789 9 26.08570495128209 17.435163598806106 20.46179873715636 36.01733271275544 10-11 25.274637998072375 26.786148429894347 26.714876943101768 21.224336628931507 12-13 26.455445393463872 24.15407617658351 27.39345901453425 21.99701941541837 14-15 22.83366963943223 25.799833939470453 26.344733058045577 25.02176336305174 16-17 21.92936242501092 29.009490322215914 26.92153619540151 22.139611057371656 18-19 21.727240630902397 27.613390202132095 29.47819247115 21.18117669581551 20-21 22.69614429266946 26.227126527990592 29.63984404316905 21.4368851361709 22-23 22.209701859291236 26.289397957828225 29.586762126630468 21.91413805625007 24-25 22.450927450974273 26.494268958875022 29.65446585600761 21.40033773414309 26-27 22.13417582257504 26.476500779341855 29.873834715708437 21.515488682374663 28-29 21.609066951907863 27.242892728638644 29.664043862770683 21.483996456682807 30-31 21.943704460033732 26.530987379474837 29.61399692507142 21.911311235420015 32-33 21.897722121663723 26.806541439253976 29.564210407301804 21.731526031780493 34-35 22.51460335177316 26.529801035076833 29.462437796363673 21.493157816786336 36-37 22.469273948422064 26.394140501294324 29.530828723226026 21.605756827057586 38-39 22.065849830476143 26.83398801626735 29.593056025250817 21.507106128005688 40-41 22.25404527096509 26.82661043490023 29.279073840609993 21.64027045352469 42-43 22.05163313959981 27.04351408885939 28.84742774183569 22.057425029705115 44-45 22.361548492709794 26.8173841266318 28.7825839657062 22.03848341495221 46-47 22.7227341444475 27.303190480269713 28.208141962204497 21.76593341307829 48-49 22.738672074286097 27.36074391530098 28.07713557094743 21.823448439465494 50-51 22.29149412275554 27.653985469548342 28.300357504047412 21.754162903648705 52-53 22.78024604008643 27.79413651860561 27.643439566880662 21.7821778744273 54-55 22.289838796552917 27.531179214019176 27.899478748121474 22.27950324130643 56-57 22.595194915862646 27.757401968077783 27.688835200797968 21.958567915261604 58-59 23.070546902366363 27.31973100546937 27.630101306475495 21.97962078568877 60-61 22.530699023839542 27.537991180046024 28.300745516754617 21.630564279359817 62-63 22.27459500405282 27.59476785504324 28.272457778346592 21.858179362557344 64-65 22.20666389645994 27.858475271742712 27.928955990479555 22.005904841317793 66-67 22.380684661787704 27.73926624106091 27.572718825858068 22.307330271293324 68-69 22.285623118436206 27.644837058309403 27.801605138663447 22.267934684590944 70-71 22.596342595225842 27.68021014401274 27.61150548180662 22.1119417789548 72-73 22.57816258149902 27.47613252375649 27.90092105724067 22.04478383750382 74-75 22.299124779148418 28.30028739319871 27.377757306398053 22.022830521254818 76-77 22.563559909879913 27.720389114559058 27.351675046058908 22.36437592950212 78-79 22.56144848759793 27.842057582681633 27.431476109416252 22.165017820304186 80-81 22.721223537474756 28.010242853292944 27.257671301246784 22.01086230798552 82-83 22.603007218378494 27.876703491542028 27.493448242852725 22.026841047226757 84-85 22.371070731174367 28.062132999441587 27.37195517433936 22.19484109504469 86-87 22.395699297667562 28.010404924997832 27.255909130321687 22.33798664701292 88-89 22.29794061915658 28.040364183637408 26.916381291902226 22.745313905303792 90-91 22.36977977018623 27.83752575059583 27.21978894483866 22.572905534379277 92-93 22.563653118294415 27.959129102081175 27.299681652038288 22.177536127586123 94-95 22.26661982201576 27.889573331011906 27.330663003257737 22.51314384371459 96-97 22.080341272662636 27.87975687791005 27.62716655250449 22.412735296922822 98-99 22.041188213864068 28.152447306028055 26.928759608941572 22.87760487116631 100-101 21.69041968109883 28.145544571282105 27.0972349250645 23.06680082255456 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 208.5 2 195.0 3 232.0 4 211.0 5 211.0 6 349.5 7 698.5 8 1117.0 9 1399.5 10 1388.0 11 1301.5 12 1424.0 13 1600.5 14 1767.5 15 1919.0 16 2047.0 17 2207.0 18 2319.5 19 2475.5 20 2783.0 21 3179.0 22 3667.5 23 4389.0 24 5330.5 25 6430.0 26 7997.0 27 10112.0 28 12208.5 29 14718.0 30 17565.5 31 20650.5 32 24031.0 33 26525.5 34 29452.0 35 32294.5 36 35306.0 37 38473.0 38 40028.5 39 40921.5 40 41837.0 41 43932.0 42 46356.5 43 48556.0 44 50799.5 45 52530.0 46 53019.0 47 56511.0 48 66247.0 49 65583.5 50 56075.0 51 51168.0 52 47963.5 53 46191.5 54 44158.0 55 41081.0 56 37383.0 57 33159.5 58 30003.5 59 26585.0 60 22376.0 61 18464.5 62 15030.0 63 11872.0 64 8728.5 65 6461.0 66 4822.5 67 3844.0 68 2954.0 69 2035.5 70 1511.0 71 1163.5 72 939.5 73 793.0 74 617.5 75 510.5 76 391.0 77 239.0 78 156.0 79 114.5 80 83.0 81 54.5 82 42.0 83 32.0 84 19.0 85 9.0 86 4.5 87 1.5 88 2.0 89 2.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03745627500119652 2 0.005202260416832851 3 0.004231171805690718 4 7.62998194468818E-4 5 9.01725138917694E-4 6 4.1618083334662797E-4 7 0.0 8 0.0 9 9.71088611142132E-4 10-11 7.28316458356599E-4 12-13 0.001213860763927665 14-15 0.002635811944528644 16-17 0.0037803092362318712 18-19 0.0 20-21 0.0 22-23 0.002046222430620921 24-25 0.001005770347254351 26-27 0.00554907777795504 28-29 0.006520166389097173 30-31 0.001491314652825417 32-33 7.28316458356599E-4 34-35 0.010092385208655729 36-37 0.013352468403204316 38-39 0.009953658264206853 40-41 0.001976858958396483 42-43 6.9363472224438E-4 44-45 0.001456632916713198 46-47 1.0404520833665699E-4 48-49 7.97679930581037E-4 50-51 0.001317905972264322 52-53 0.003190719722324148 54-55 0.003849672708456309 56-57 0.001421951180600979 58-59 0.001109815555591008 60-61 0.004404580486251813 62-63 0.0064161211807605145 64-65 0.010543247778114577 66-67 0.003433491875109681 68-69 0.004231171805690718 70-71 2.0809041667331398E-4 72-73 0.0015606781250498553 74-75 0.0070750741668926765 76-77 0.0033988101389974624 78-79 0.004647352639037346 80-81 0.006277394236311639 82-83 0.005757168194628354 84-85 0.0066588933335460475 86-87 0.003884354444568528 88-89 0.01078601993090011 90-91 0.01453164743101976 92-93 0.001352587708376541 94-95 0.002531766736191987 96-97 0.001283224236152103 98-99 0.00381499097234409 100-101 0.005167578680720631 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1441681.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.19015083790848 #Duplication Level Percentage of deduplicated Percentage of total 1 82.24646979748618 52.794133021861235 2 10.970757658301961 14.084291777850853 3 3.070316908961501 5.9125231651925905 4 1.2481655965544034 3.204797516540607 5 0.6575225241095496 2.1103235000957152 6 0.38345064516606536 1.4768252851261836 7 0.2553366398050548 1.147306819647182 8 0.1787768606800649 0.9180570922704894 9 0.13937585918302042 0.8051901681709056 >10 0.7682853277826234 9.056960542603143 >50 0.053111767853275885 2.346952223249301 >100 0.025619868103355762 3.148120856570893 >500 0.0012971750829287984 0.5567016906403706 >1k 0.0011890771593513984 1.242881800168306 >5k 3.242937707321996E-4 1.1949345400121927 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6517 0.45204174848666245 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5675 0.3936377048736856 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5029 0.3488289018166987 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3087 0.21412503875684008 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2342 0.1624492519496338 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1885 0.13075014514306563 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1575 0.10924746875348985 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1548 0.10737465500343002 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1445 0.1002302173643129 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.9363472224438E-5 2 0.0 0.0 0.0 0.0 6.9363472224438E-5 3 0.0 0.0 0.0 0.0 6.9363472224438E-5 4 0.0 0.0 0.0 0.0 6.9363472224438E-5 5 0.0 0.0 0.0 0.0 6.9363472224438E-5 6 0.0 0.0 0.0 0.0 6.9363472224438E-5 7 0.0 0.0 0.0 0.0 6.9363472224438E-5 8 0.0 0.0 0.0 0.0 6.9363472224438E-5 9 0.0 0.0 0.0 0.0 6.9363472224438E-5 10-11 0.0 0.0 0.0 0.0 6.9363472224438E-5 12-13 0.0 0.0 0.0 0.0 6.9363472224438E-5 14-15 6.9363472224438E-5 0.0 0.0 0.0 6.9363472224438E-5 16-17 1.0404520833665699E-4 0.0 0.0 0.0 6.9363472224438E-5 18-19 1.38726944448876E-4 0.0 0.0 3.4681736112219E-5 6.9363472224438E-5 20-21 1.38726944448876E-4 0.0 0.0 2.08090416673314E-4 6.9363472224438E-5 22-23 1.38726944448876E-4 0.0 0.0 5.20226041683285E-4 6.9363472224438E-5 24-25 1.38726944448876E-4 0.0 0.0 6.9363472224438E-4 6.9363472224438E-5 26-27 1.38726944448876E-4 0.0 0.0 0.001075133819478789 6.9363472224438E-5 28-29 2.08090416673314E-4 0.0 0.0 0.0019074954861720448 6.9363472224438E-5 30-31 2.08090416673314E-4 0.0 0.0 0.003710945764007433 1.0404520833665699E-4 32-33 2.08090416673314E-4 0.0 0.0 0.005410350833506165 1.73408680561095E-4 34-35 2.77453888897752E-4 0.0 0.0 0.007317846319678209 2.77453888897752E-4 36-37 3.4681736112219E-4 0.0 0.0 0.010023021736431291 2.77453888897752E-4 38-39 3.4681736112219E-4 0.0 0.0 0.01491314652825417 2.77453888897752E-4 40-41 3.4681736112219E-4 0.0 0.0 0.020600951250658087 2.77453888897752E-4 42-43 3.4681736112219E-4 0.0 0.0 0.026878345486969726 3.4681736112219E-4 44-45 3.4681736112219E-4 0.0 0.0 0.03228869632047589 3.4681736112219E-4 46-47 3.4681736112219E-4 0.0 0.0 0.04480880305698695 3.4681736112219E-4 48-49 3.4681736112219E-4 0.0 0.0 0.06180285375197425 3.4681736112219E-4 50-51 3.4681736112219E-4 0.0 0.0 0.06884324618275471 3.4681736112219E-4 52-53 3.4681736112219E-4 0.0 0.0 0.0797679930581037 4.85544305571066E-4 54-55 3.4681736112219E-4 0.0 0.0 0.09866953923926305 4.85544305571066E-4 56-57 3.4681736112219E-4 0.0 0.0 0.12790624278186366 5.20226041683285E-4 58-59 3.4681736112219E-4 0.0 0.0 0.1640792935469081 5.54907777795504E-4 60-61 3.4681736112219E-4 0.0 0.0 0.20528119604822426 5.54907777795504E-4 62-63 3.4681736112219E-4 0.0 0.0 0.24835591229960025 5.54907777795504E-4 64-65 3.4681736112219E-4 0.0 0.0 0.29760397757895124 5.54907777795504E-4 66-67 4.16180833346628E-4 0.0 0.0 0.36537208994222714 5.54907777795504E-4 68-69 4.16180833346628E-4 0.0 0.0 0.45169493112554027 5.54907777795504E-4 70-71 4.16180833346628E-4 0.0 0.0 0.4999025443215247 5.54907777795504E-4 72-73 4.16180833346628E-4 0.0 0.0 0.5609423998790302 5.54907777795504E-4 74-75 4.16180833346628E-4 0.0 0.0 0.6279821957839495 5.54907777795504E-4 76-77 4.16180833346628E-4 0.0 0.0 0.7009872503001704 5.54907777795504E-4 78-79 4.16180833346628E-4 0.0 0.0 0.79022335731691 5.54907777795504E-4 80-81 4.85544305571066E-4 0.0 0.0 0.9206960485710778 5.54907777795504E-4 82-83 4.85544305571066E-4 0.0 0.0 1.0568218628115376 5.54907777795504E-4 84-85 4.85544305571066E-4 0.0 0.0 1.2702185851100207 5.54907777795504E-4 86-87 4.85544305571066E-4 0.0 0.0 1.5688630147723388 5.54907777795504E-4 88-89 4.85544305571066E-4 0.0 0.0 1.9034030413108032 5.54907777795504E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6405 0.0 23.437786 1 GAGTACT 6180 0.0 22.827394 12-13 TACATGG 6465 0.0 22.479988 2 AGTACTT 6430 0.0 21.31195 12-13 ACATGGG 6450 0.0 21.20537 3 GTACTTT 6740 0.0 21.14218 14-15 CGTATAC 115 0.0037277336 20.648346 3 GTAAGGT 515 0.0 20.287498 4 GTGTAGC 705 0.0 20.215326 1 TACCTGG 1185 0.0 20.039867 2 CGGTTAC 160 3.965397E-10 19.296307 28-29 GTATCAA 14380 0.0 19.227053 1 AGAGTAC 10550 0.0 18.932892 10-11 TAAGGTG 670 0.0 18.430056 5 GTACACG 235 2.5252648E-6 18.193794 1 GTACCTG 1315 0.0 18.063126 1 ACTTTTT 7975 0.0 17.985996 16-17 TACTTTT 7685 0.0 17.862505 14-15 TACCGCT 135 0.009409696 17.58994 7 CATGGGG 2850 0.0 17.496754 4 >>END_MODULE