FastQCFastQC Report
Thu 26 May 2016
SRR936668_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936668_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475865
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53860.3649385275753541No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42220.2860695253292137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41800.28322373658837363No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34280.23227056675237912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.17359311319124715No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21440.1452707395324098No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21350.14466092765937263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG63950.025.043041
TACATGG64400.024.940152
TACCTGG12750.024.5981242
GTACCTG14000.024.4400521
ACATGGG65200.023.540113
GAGTACT54250.021.85996612-13
GTATCAA114150.021.1071571
CGTACAC1404.8405994E-420.3646413
GTACTTT60450.019.61459414-15
GGTATCA88700.019.2875291
AGAGTAC89100.019.20582410-11
CATGGGA34300.018.702224
AGTACTT58600.018.49336412-13
CAACGCA129100.018.44085
TCAACGC129300.018.411654
TGTACCG1559.6003216E-418.3944915
ATCAACG129500.018.3465233
AACGCAG131450.018.2918726
TATCAAC132050.018.1367872
GTCTACG1350.00937622417.600861
Produced by FastQC (version 0.11.5)