##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936668_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1475865 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.422910632070007 31.0 31.0 34.0 30.0 34.0 2 31.489640990198968 33.0 31.0 34.0 28.0 34.0 3 31.44567897470297 33.0 31.0 34.0 28.0 34.0 4 35.09830370663983 37.0 35.0 37.0 32.0 37.0 5 35.13463765317289 37.0 35.0 37.0 32.0 37.0 6 35.19034938832481 37.0 35.0 37.0 32.0 37.0 7 35.06637056912387 37.0 35.0 37.0 32.0 37.0 8 35.04794747487067 37.0 35.0 37.0 32.0 37.0 9 36.557314524024896 39.0 37.0 39.0 32.0 39.0 10-11 36.438269760445564 39.0 37.0 39.0 32.0 39.0 12-13 36.34492788974602 39.0 37.0 39.0 32.0 39.0 14-15 37.351739827152215 40.0 37.0 41.0 32.0 41.0 16-17 37.274869991496516 40.0 37.0 41.0 32.0 41.0 18-19 37.1649469971847 40.0 37.0 41.0 31.0 41.0 20-21 37.15450938940892 39.5 36.5 41.0 31.0 41.0 22-23 37.14334949334797 39.0 36.5 41.0 31.0 41.0 24-25 37.2630745359501 40.0 37.0 41.0 31.0 41.0 26-27 37.18729829625339 40.0 37.0 41.0 31.0 41.0 28-29 37.07476429077185 39.0 36.0 41.0 31.0 41.0 30-31 36.88260037334038 39.0 36.0 41.0 30.0 41.0 32-33 36.707756129456286 39.0 36.0 41.0 30.0 41.0 34-35 36.51543738756594 39.0 35.0 41.0 30.0 41.0 36-37 36.309336558560574 39.0 35.0 40.5 30.0 41.0 38-39 36.11941844274375 39.0 35.0 40.0 29.5 41.0 40-41 35.899333949920894 38.5 35.0 40.0 28.5 41.0 42-43 35.63629329240818 38.0 35.0 40.0 27.5 41.0 44-45 35.47003011793085 38.0 35.0 40.0 27.0 41.0 46-47 35.31634837874738 38.0 35.0 40.0 26.0 41.0 48-49 35.08063034220609 38.5 34.5 40.0 25.0 41.0 50-51 34.11303608392367 37.5 33.5 39.5 23.5 40.5 52-53 34.22840876367418 38.0 33.5 39.5 24.0 40.5 54-55 34.67849261280673 38.0 34.0 40.0 24.0 41.0 56-57 34.52499686624454 38.0 34.0 40.0 23.5 41.0 58-59 34.31087396204937 38.0 34.0 40.0 23.0 41.0 60-61 34.02404081674137 38.0 33.5 40.0 23.0 41.0 62-63 33.69849308710485 37.0 33.0 40.0 22.0 41.0 64-65 33.34990530976749 37.0 33.0 39.5 21.5 41.0 66-67 32.98378577986469 36.0 32.0 39.0 20.0 41.0 68-69 32.541316787104506 36.0 32.0 39.0 18.5 40.5 70-71 32.13889075220294 35.0 31.0 38.5 18.0 40.0 72-73 31.729973269912897 35.0 31.0 37.5 16.0 40.0 74-75 31.32213955883499 35.0 31.0 37.0 13.0 39.0 76-77 30.863342514389867 35.0 30.5 36.5 9.0 39.0 78-79 30.390835882685746 34.5 30.0 36.0 7.0 39.0 80-81 29.92350723135246 34.0 29.5 36.0 4.5 37.5 82-83 29.52061807821176 34.0 29.0 35.0 2.0 37.0 84-85 29.145448940113084 34.0 29.0 35.0 2.0 36.5 86-87 28.813731947027676 34.0 29.0 35.0 2.0 36.0 88-89 28.45452463470575 34.0 29.0 35.0 2.0 36.0 90-91 28.090167461116025 34.0 27.5 35.0 2.0 35.5 92-93 27.667126397062063 34.0 27.0 35.0 2.0 35.0 94-95 27.193174511218846 33.0 26.0 35.0 2.0 35.0 96-97 26.61929546401602 33.0 25.0 35.0 2.0 35.0 98-99 25.896920788825536 33.0 24.0 35.0 2.0 35.0 100-101 23.34805825736094 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43740182837801456 1101 2 0.4455110666951718 1101 3 0.48653252121062707 1101 4 0.6103291226056768 1101 5 0.27940877983900236 1101 6 0.3263804181987311 1101 7 0.4825803207664947 1101 8 0.35055847718957267 1101 9 0.5614045959603402 1101 10-11 0.562861570380484 1101 12-13 0.6304959844379923 1101 14-15 0.7643304776771487 1101 16-17 0.8377998149662389 1101 18-19 0.842080991812395 1101 20-21 0.7989310766286337 1101 22-23 0.6095786337495213 1101 24-25 0.5694791385773641 1101 26-27 0.5439587559244714 1101 28-29 0.5769057903748873 1101 30-31 0.554772183317219 1101 32-33 0.5557285841688966 1101 34-35 0.62026470131255 1101 36-37 0.6475475971246141 1101 38-39 0.7480346446578423 1101 40-41 0.6879478684047484 1101 42-43 0.6659209234813446 1101 44-45 0.7039332874496012 1101 46-47 0.8995549614947222 1101 48-49 0.9652324230480218 1101 50-51 0.9414810815168266 1101 52-53 0.8788170556183772 1101 54-55 0.8484281765402102 1101 56-57 0.9051111010380595 1101 58-59 0.962729626827624 1101 60-61 0.8017768239342686 1101 62-63 0.9613530878066712 1101 64-65 0.7711042208079775 1101 66-67 0.7443230343726412 1101 68-69 0.8776177308220028 1101 70-71 0.74625309599422 1101 72-73 0.8952897664649306 1101 74-75 0.7360559859783642 1101 76-77 0.853459672178225 1101 78-79 0.6642439081227742 1101 80-81 0.8233139006422938 1101 82-83 0.8280036091264478 1101 84-85 0.8539180748101955 1101 86-87 0.8707635321033642 1101 88-89 1.0457137630837146 1101 90-91 0.8466787400518072 1101 92-93 0.8732429760176288 1101 94-95 0.8046581463536349 1101 96-97 0.8199344466516223 1101 98-99 0.623480370641289 1101 100-101 0.9505109632104087 1102 1 0.20507815357482073 1102 2 0.44043889950518533 1102 3 0.4472715874177098 1102 4 0.32295981972090715 1102 5 0.2453533482747261 1102 6 0.3807197222985437 1102 7 0.3305633485270363 1102 8 0.22135778397527162 1102 9 0.4610736564758966 1102 10-11 0.409476235316248 1102 12-13 0.2913504797792612 1102 14-15 0.49463302351306737 1102 16-17 0.2787287157355465 1102 18-19 0.4136905407754554 1102 20-21 0.3350819786385699 1102 22-23 0.22338584471305012 1102 24-25 0.4698494071019823 1102 26-27 0.36676576224909496 1102 28-29 0.23881225760388958 1102 30-31 0.18141441055593788 1102 32-33 0.3593785109465344 1102 34-35 -0.05411035064653902 1102 36-37 0.05329075610985967 1102 38-39 0.07584539203750751 1102 40-41 0.29155910563880383 1102 42-43 0.06595002057611055 1102 44-45 0.21136008107766457 1102 46-47 0.314335806013915 1102 48-49 0.5395596726371537 1102 50-51 0.34535930857647656 1102 52-53 0.34946016536238744 1102 54-55 0.44038075147322076 1102 56-57 0.4810577158628746 1102 58-59 0.2195613688802851 1102 60-61 0.22440796869710766 1102 62-63 0.2742750756178651 1102 64-65 0.38613446899660175 1102 66-67 0.2828199296167 1102 68-69 0.4747016263794208 1102 70-71 0.22734494049423404 1102 72-73 0.4498951011454686 1102 74-75 0.12823718094168868 1102 76-77 0.31123643031422077 1102 78-79 0.3381482905928941 1102 80-81 0.587902736310852 1102 82-83 0.424873593075084 1102 84-85 0.4115127362926785 1102 86-87 0.4687088295652053 1102 88-89 0.4936350794234947 1102 90-91 0.5625783838522089 1102 92-93 0.5086973063687772 1102 94-95 0.5320973462155045 1102 96-97 0.3999636076960478 1102 98-99 0.5078008735414059 1102 100-101 1.0192963353654498 1103 1 0.37750537522957117 1103 2 0.40709492606289643 1103 3 0.5413088703594866 1103 4 0.4626623222745323 1103 5 0.5066850643114975 1103 6 0.4898233995201977 1103 7 0.5857727458712674 1103 8 0.3386777533317584 1103 9 0.6613609394176763 1103 10-11 0.5062267460414773 1103 12-13 0.5497391416791615 1103 14-15 0.8609538866387041 1103 16-17 0.9815760088918282 1103 18-19 1.0263916388346956 1103 20-21 0.9387049357376469 1103 22-23 0.7377340316384888 1103 24-25 0.7679469556208787 1103 26-27 0.8241042453951621 1103 28-29 0.8744991830380044 1103 30-31 0.8550258763373506 1103 32-33 0.8261334905175275 1103 34-35 0.808514138628226 1103 36-37 0.9147948419117 1103 38-39 0.7117206474001208 1103 40-41 0.9876584416960057 1103 42-43 0.7593042085229982 1103 44-45 0.8117558931307798 1103 46-47 1.0274805966165772 1103 48-49 1.1252721343327607 1103 50-51 1.1358662033670868 1103 52-53 1.2344799559650426 1103 54-55 1.1309387187868012 1103 56-57 1.1894751060569462 1103 58-59 1.0760443872663856 1103 60-61 1.1664470391771928 1103 62-63 1.2143670061592289 1103 64-65 1.2307080134296982 1103 66-67 1.321906369861857 1103 68-69 1.227047770403935 1103 70-71 1.2576915952439691 1103 72-73 1.284397782452924 1103 74-75 1.1042809756301466 1103 76-77 1.1080922781181002 1103 78-79 1.0529210178656214 1103 80-81 1.1261400958614125 1103 82-83 1.0562097779576192 1103 84-85 1.1054510662211783 1103 86-87 1.0884643198614263 1103 88-89 0.9776653398933597 1103 90-91 1.0287045329839763 1103 92-93 1.1389130877783806 1103 94-95 1.420950768074544 1103 96-97 1.573846396255803 1103 98-99 1.5190527887610195 1103 100-101 1.9211955948138701 1104 1 0.40638189954003323 1104 2 0.452659051775985 1104 3 0.6064466470905288 1104 4 0.5922656744491306 1104 5 0.5434415540796493 1104 6 0.3948523156242416 1104 7 0.5304841081992109 1104 8 0.5636080799136423 1104 9 0.778164663183837 1104 10-11 0.7418187411218113 1104 12-13 0.8282777662670142 1104 14-15 0.7022226297694942 1104 16-17 0.6614570734231577 1104 18-19 0.7306369743138461 1104 20-21 0.9012070619217667 1104 22-23 0.6371990510031154 1104 24-25 0.7981171811926586 1104 26-27 0.373814593696828 1104 28-29 0.40538642331643615 1104 30-31 0.4833981679058752 1104 32-33 0.553952974214603 1104 34-35 0.616065853968152 1104 36-37 0.5894775498197973 1104 38-39 0.5483134209909579 1104 40-41 0.7835509810794719 1104 42-43 0.876227652810428 1104 44-45 0.894807261739146 1104 46-47 0.969122730389536 1104 48-49 1.0244537169987922 1104 50-51 0.9479373683851406 1104 52-53 0.7209137564046699 1104 54-55 0.8118956324833633 1104 56-57 0.9568069412482316 1104 58-59 1.001171467655773 1104 60-61 0.9255742569507817 1104 62-63 0.9503014804356766 1104 64-65 0.915691919851426 1104 66-67 0.8813536601219667 1104 68-69 0.7651753218248771 1104 70-71 0.75275181532038 1104 72-73 0.8780949549551487 1104 74-75 0.7884413945865489 1104 76-77 0.7822511269693102 1104 78-79 0.6774977436729195 1104 80-81 0.8069097418026914 1104 82-83 0.6994568536366899 1104 84-85 0.6959903646458372 1104 86-87 0.7520593046837405 1104 88-89 0.7575357735040775 1104 90-91 1.055696603952729 1104 92-93 0.8251547138839932 1104 94-95 0.9407164298738877 1104 96-97 1.1086121234761919 1104 98-99 1.2527935316824603 1104 100-101 1.7521615039047767 1105 1 0.13551026590851123 1105 2 0.10865562801916795 1105 3 0.26295731684970747 1105 4 0.305952023080053 1105 5 0.36013011609516354 1105 6 0.40663870446841344 1105 7 0.40245859167103504 1105 8 0.20024996276789153 1105 9 0.23717365518292155 1105 10-11 0.3744013720483821 1105 12-13 0.37800438908998046 1105 14-15 0.5805777358907207 1105 16-17 0.6184143287997657 1105 18-19 0.3976337274422903 1105 20-21 0.47000168487458893 1105 22-23 0.3623564424555141 1105 24-25 0.06367480372214374 1105 26-27 0.25558799107985664 1105 28-29 0.3010095397698791 1105 30-31 0.039623833759669935 1105 32-33 0.1225985365474429 1105 34-35 0.18766761964087664 1105 36-37 0.1986124483558882 1105 38-39 -0.15053841474026797 1105 40-41 0.16007645090198963 1105 42-43 -0.17781460620658152 1105 44-45 -0.07830452113849873 1105 46-47 0.03748491192151704 1105 48-49 0.17462483859051758 1105 50-51 0.2779332344372847 1105 52-53 0.3521834945150957 1105 54-55 0.1750195588328367 1105 56-57 0.14127674587282257 1105 58-59 0.2254366484861876 1105 60-61 0.08014813238777663 1105 62-63 0.2025718393007807 1105 64-65 0.22283977407411726 1105 66-67 0.21375403959718398 1105 68-69 0.25238939871233157 1105 70-71 0.5253088276950706 1105 72-73 0.3070135764690356 1105 74-75 0.23721754064740708 1105 76-77 0.26553977524122985 1105 78-79 0.36910341142143466 1105 80-81 0.29502315109731825 1105 82-83 0.16455629694495855 1105 84-85 0.20187295920161574 1105 86-87 0.26673316335969943 1105 88-89 0.30320115692443395 1105 90-91 0.16410441790917574 1105 92-93 0.13877643645501792 1105 94-95 0.26144271283864384 1105 96-97 0.5187615285918206 1105 98-99 0.6345195609129171 1105 100-101 0.9788588571844095 1106 1 0.14483921009389533 1106 2 0.12000472967391929 1106 3 0.17077117634618233 1106 4 0.2962127657335145 1106 5 0.4138998515567778 1106 6 0.19318497696713877 1106 7 0.24106785726394264 1106 8 0.39505181626070396 1106 9 0.4846893894229538 1106 10-11 0.4866333820883568 1106 12-13 0.5800936448995131 1106 14-15 0.567369193892425 1106 16-17 0.4556640143822719 1106 18-19 0.5659416467536076 1106 20-21 0.4020921836523357 1106 22-23 0.24442716066393189 1106 24-25 -0.03584752225896182 1106 26-27 -0.046529465406230486 1106 28-29 -0.01644554296769485 1106 30-31 0.08920356348759384 1106 32-33 0.30738514029051345 1106 34-35 0.18537013324336726 1106 36-37 -0.04097936355762499 1106 38-39 0.10323058563262322 1106 40-41 0.14126352563645383 1106 42-43 0.22981748589602802 1106 44-45 0.216830594851821 1106 46-47 0.3548938845481544 1106 48-49 0.19520799115885268 1106 50-51 0.1427734729267911 1106 52-53 0.21382776770614242 1106 54-55 0.19625059187307414 1106 56-57 0.34523446356935494 1106 58-59 0.28131475206419765 1106 60-61 0.5357711601781432 1106 62-63 0.38399006602619323 1106 64-65 0.3498704566775075 1106 66-67 0.17625616133054933 1106 68-69 0.40735245047995505 1106 70-71 0.25474873018220734 1106 72-73 0.3046870206537484 1106 74-75 0.4867026444085205 1106 76-77 0.19610997688275233 1106 78-79 0.2900646668995144 1106 80-81 0.4857038298916123 1106 82-83 0.5295134813680278 1106 84-85 0.6798119441643706 1106 86-87 0.7851327682829208 1106 88-89 0.6469106017453292 1106 90-91 0.8848535668769095 1106 92-93 0.7013926305932863 1106 94-95 0.8374445385317308 1106 96-97 0.8861411644956938 1106 98-99 0.915628849895004 1106 100-101 1.5343914690615712 1107 1 0.28869168096205655 1107 2 0.1520102416390401 1107 3 0.23982508344576559 1107 4 0.3016642041506188 1107 5 0.3789672009646594 1107 6 0.3011363699892229 1107 7 0.3591369612736983 1107 8 0.3674089345709035 1107 9 0.32733879118374176 1107 10-11 0.5801012393825289 1107 12-13 0.4506004618876176 1107 14-15 0.3042424650984614 1107 16-17 0.33724335110375847 1107 18-19 0.522238844972513 1107 20-21 0.30818416272086324 1107 22-23 0.42571354814108986 1107 24-25 0.3534782237158396 1107 26-27 0.27293350133030714 1107 28-29 0.395872952580568 1107 30-31 0.31072759897776336 1107 32-33 0.377311472567321 1107 34-35 0.37406313978630124 1107 36-37 0.3889769496129176 1107 38-39 0.4933307371511333 1107 40-41 0.5420844694898648 1107 42-43 0.5778804968843119 1107 44-45 0.3943340633681842 1107 46-47 0.41316748138785186 1107 48-49 0.6437507098973185 1107 50-51 0.5757149294817339 1107 52-53 0.6308917249285599 1107 54-55 0.5906484755905126 1107 56-57 0.7552521037407871 1107 58-59 0.7917164341685279 1107 60-61 0.618976714915469 1107 62-63 0.7071290212142358 1107 64-65 0.712605813082277 1107 66-67 0.7342091347721009 1107 68-69 0.6532583233859768 1107 70-71 0.8607188824472161 1107 72-73 0.7900198872589463 1107 74-75 0.6594724203319444 1107 76-77 0.5992420320266163 1107 78-79 0.7807944978887846 1107 80-81 0.7510840014780875 1107 82-83 0.6051150327346875 1107 84-85 0.5840972011372152 1107 86-87 0.908670964331602 1107 88-89 0.8089750532691546 1107 90-91 0.826612755334871 1107 92-93 0.8102470484037418 1107 94-95 1.0051502469396318 1107 96-97 1.0646650123530819 1107 98-99 1.5223949283093674 1107 100-101 2.002306263603675 1108 1 -0.0037453345540860994 1108 2 0.0606548459992986 1108 3 0.18601317448758792 1108 4 0.15465489793746912 1108 5 0.17239719025551636 1108 6 0.08009581448811787 1108 7 0.21264231602628314 1108 8 0.24832477791866836 1108 9 -0.04759262653541896 1108 10-11 0.08267364002819022 1108 12-13 0.023916502419602637 1108 14-15 0.38833148444689414 1108 16-17 0.5380513652032235 1108 18-19 0.43601577289863513 1108 20-21 0.39505625735482397 1108 22-23 0.2583718787226914 1108 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>>Per sequence quality scores pass #Quality Count 2 3880.0 3 1361.0 4 1351.0 5 1520.0 6 1637.0 7 1867.0 8 2364.0 9 2972.0 10 3828.0 11 4775.0 12 6127.0 13 7379.0 14 8240.0 15 10170.0 16 12061.0 17 15202.0 18 12064.0 19 9269.0 20 9400.0 21 10197.0 22 11259.0 23 12356.0 24 14630.0 25 16566.0 26 19467.0 27 22823.0 28 26904.0 29 32888.0 30 39189.0 31 48456.0 32 60554.0 33 78148.0 34 103469.0 35 140735.0 36 199917.0 37 269823.0 38 219709.0 39 33299.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3567783008665 18.338037967516666 14.622653983253176 22.68252974836366 2 15.258774881678802 19.652197016062402 40.839672150480574 24.249355951778224 3 19.91766340256836 23.957510249720464 29.828821197438415 26.296005150272762 4 12.613423423728698 16.145856982626878 37.65667833459275 33.58404125905168 5 13.586534779617827 37.00522871830322 34.4790427684081 14.929193733670845 6 31.730098623232955 35.58041036645332 17.744523058967218 14.944967951346506 7 28.391423456656177 32.58523651947488 20.719051752197025 18.30428827167191 8 26.16630384746543 34.09709543512203 19.88274081149921 19.853859905913332 9 25.998075753476762 17.118200131130823 21.02235936490372 35.86136475048869 10-11 24.97677116281813 26.29426402806511 27.288604022309176 21.44036078680758 12-13 26.158370413500492 23.656225926416727 27.8679612782397 22.317442381843083 14-15 22.814487226503743 25.084378217777488 26.727677478963912 25.373457076754864 16-17 21.829327819395953 28.41554523575472 27.290634215789293 22.46449272906003 18-19 21.59071342089243 26.966046106142016 29.81778406879992 21.625456404165632 20-21 22.612272365018352 25.84951423413264 29.7283560403576 21.809857360491407 22-23 22.06433149981466 25.879143629092976 29.83904249530213 22.217482375790233 24-25 22.225622112070308 26.002922323925237 29.86544568828454 21.906009875719906 26-27 21.90326572714716 26.048284452926655 30.079681139893705 21.968768680032476 28-29 21.499856007860277 26.658975792380275 29.934746150327797 21.906422049431654 30-31 21.737405562895958 26.049395263746316 29.883321475759733 22.329877697597993 32-33 21.5799930610519 26.226479405418885 29.912490648683228 22.281036884845985 34-35 22.375655789583533 26.17079871139295 29.548648710918602 21.904896788104917 36-37 22.168616899093173 25.93625047177578 29.829476347516987 22.06565628161406 38-39 21.811624843467936 26.322966980543942 29.8158003870611 22.049607788927016 40-41 22.098602013888723 26.300657642643678 29.648160677435275 21.95257966603232 42-43 21.804352326129372 26.40694415317738 29.273226652229607 22.515476868463644 44-45 22.034433471185118 26.352660024439977 29.190699256467667 22.422207247907238 46-47 22.354446581430402 26.6588226445828 28.76102282163229 22.22570795235451 48-49 22.310931629462242 26.712109251925153 28.61554827540646 22.36141084320614 50-51 21.954515671996255 26.98321440743599 28.806368402187488 22.255901518380266 52-53 22.355338176370527 27.171329075264023 28.185480442140857 22.28785230622459 54-55 21.94643603075221 26.784762662350094 28.53794207654309 22.73085923035461 56-57 22.297969856492074 27.041510170367804 28.177973136053698 22.48254683708642 58-59 22.580778492857558 26.73702726473175 28.220061172523046 22.462133069887642 60-61 22.31794467319129 26.757282263892435 28.865976936490817 22.058796126425463 62-63 22.032492037577285 26.952169902059065 28.743050137260955 22.272287923102695 64-65 21.89316737359912 27.20112341278983 28.453173065195887 22.452536148415163 66-67 21.96310758683587 27.071081993605077 28.09367290506749 22.872137514491563 68-69 21.92530073295293 26.961480840026265 28.411090580026848 22.702127846993957 70-71 22.221156908315997 26.881788552882107 28.212023064987157 22.685031473814735 72-73 22.086181231538994 26.751815307440857 28.531458721387875 22.63054473963227 74-75 21.8697903819099 27.44433586602658 28.116181768403592 22.569691983659926 76-77 22.01264915998707 27.01063968938261 28.119237173782945 22.857473976847377 78-79 22.14078531831551 27.046280106805458 28.15814798248824 22.65478659239079 80-81 22.263784883411653 27.21311853117555 27.920180716257924 22.60291586915487 82-83 22.13449997187979 27.110184461789384 28.275614629826258 22.47970093650457 84-85 22.059341683911246 27.187114199099348 28.06479219094934 22.688751926040062 86-87 22.0716342405992 27.215672111797684 28.06562615505902 22.6470674925441 88-89 22.048336491418624 27.089663761318544 27.76971646345492 23.092283283807916 90-91 21.984348392928656 26.97422140064875 28.0958476282374 22.945582578185192 92-93 22.25143972729124 26.95570651078275 28.164985954251957 22.62786780767405 94-95 21.870406725654842 26.9221021330751 28.260653008173275 22.946838133096783 96-97 21.621523637789156 26.98071455653035 28.487661944496118 22.91009986118438 98-99 21.70615452075442 27.185217113730303 27.878770627515642 23.22985773799963 100-101 21.550237295394986 26.893500688200696 28.206571350926783 23.349690665477535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 136.0 1 115.5 2 163.0 3 228.0 4 187.5 5 181.5 6 328.5 7 672.5 8 1175.5 9 1461.0 10 1377.0 11 1266.5 12 1315.0 13 1422.5 14 1547.0 15 1699.5 16 1776.5 17 1899.5 18 2082.5 19 2252.0 20 2539.5 21 2947.0 22 3515.0 23 4317.5 24 5173.5 25 6328.5 26 8117.0 27 10200.5 28 12380.5 29 14780.0 30 17652.0 31 20905.5 32 24485.0 33 27303.5 34 29874.5 35 32724.0 36 35946.0 37 38770.0 38 40389.5 39 41712.5 40 43080.0 41 45472.5 42 47480.0 43 49595.5 44 52012.5 45 53881.0 46 55168.0 47 59007.0 48 67436.5 49 66547.0 50 57788.0 51 52792.0 52 49466.0 53 48041.0 54 46270.5 55 42699.0 56 38972.0 57 34692.0 58 31194.5 59 27503.5 60 22959.0 61 18844.0 62 15267.0 63 11894.5 64 8816.0 65 6786.5 66 5186.5 67 4042.5 68 2995.5 69 2117.5 70 1575.5 71 1156.5 72 928.5 73 805.0 74 632.0 75 456.5 76 337.5 77 224.5 78 147.0 79 98.5 80 62.5 81 41.0 82 27.0 83 18.0 84 17.0 85 14.5 86 10.0 87 8.0 88 5.0 89 2.5 90 2.0 91 2.5 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07304191101489635 2 0.029270969905784063 3 0.014567728078110126 4 0.02100463118239134 5 0.02439247492148672 6 0.030626107401422215 7 0.07758162162528415 8 0.05705128856636617 9 0.06789238853147138 10-11 0.058711331998522895 12-13 0.03865529706307826 14-15 0.0448550511056228 16-17 0.05522185294725466 18-19 0.05061438546208495 20-21 0.017413516818950245 22-23 0.013280347457253882 24-25 0.022156498053683773 26-27 0.024324718046704812 28-29 0.00609811873037168 30-31 0.0010163531217286133 32-33 0.009282691845121335 34-35 0.011044370589450932 36-37 0.003929898737350638 38-39 0.008740636846866075 40-41 0.0043025615486511305 42-43 0.002269855305193903 44-45 3.0490593651858405E-4 46-47 4.404196860823991E-4 48-49 6.775687478190756E-4 50-51 0.001084109996510521 52-53 3.387843739095378E-5 54-55 2.3714906173667646E-4 56-57 0.003929898737350638 58-59 0.002642518116494395 60-61 0.004776859672124483 62-63 0.0017955571817205504 64-65 3.387843739095378E-4 66-67 4.065412486914453E-4 68-69 0.003658871238223008 70-71 0.0023714906173667645 72-73 0.01954785837458033 74-75 0.01551632432505683 76-77 0.02385041992323146 78-79 0.019310709312843656 80-81 0.01229787277291622 82-83 0.003929898737350638 84-85 0.002642518116494395 86-87 0.0013890159330291049 88-89 0.0015922865573748278 90-91 0.007182228726882202 92-93 0.010265166529458995 94-95 0.013686888705945326 96-97 0.01128151965118761 98-99 0.023579392424103833 100-101 0.0188364111893703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1475865.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.48473977780785 #Duplication Level Percentage of deduplicated Percentage of total 1 83.3553295718547 55.4185739567817 2 10.456888053793943 13.904469622843157 3 2.845116958595499 5.674705818889495 4 1.1133223843093407 2.960757960384604 5 0.5896318204974783 1.9600759075244976 6 0.33941739843269725 1.3539646446515041 7 0.23638591278901383 1.1001239129242013 8 0.16524080474869268 0.878879352351391 9 0.12642245802717808 0.7564647801606993 >10 0.6997330661599284 8.50830938851897 >50 0.04799897681987119 2.158430123094298 >100 0.022265680182940666 2.8080313159488446 >500 9.191920044517891E-4 0.40245805969079995 >1k 0.001225589339269052 1.749032343768454 >5k 1.0213244493908768E-4 0.36572281246746224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5386 0.3649385275753541 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4222 0.2860695253292137 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4180 0.28322373658837363 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3428 0.23227056675237912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2562 0.17359311319124715 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2144 0.1452707395324098 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2135 0.14466092765937263 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.775687478190756E-5 2 0.0 0.0 0.0 0.0 6.775687478190756E-5 3 0.0 0.0 0.0 0.0 6.775687478190756E-5 4 0.0 0.0 0.0 0.0 6.775687478190756E-5 5 0.0 0.0 0.0 0.0 6.775687478190756E-5 6 0.0 0.0 0.0 0.0 1.3551374956381513E-4 7 0.0 0.0 0.0 0.0 1.3551374956381513E-4 8 0.0 0.0 0.0 0.0 1.3551374956381513E-4 9 0.0 0.0 0.0 0.0 1.3551374956381513E-4 10-11 6.775687478190756E-5 0.0 0.0 0.0 1.3551374956381513E-4 12-13 1.0163531217286134E-4 0.0 0.0 0.0 2.032706243457227E-4 14-15 2.032706243457227E-4 0.0 0.0 0.0 2.032706243457227E-4 16-17 2.032706243457227E-4 0.0 0.0 0.0 2.032706243457227E-4 18-19 2.7102749912763025E-4 0.0 0.0 0.0 2.032706243457227E-4 20-21 2.7102749912763025E-4 0.0 0.0 0.0 3.387843739095378E-4 22-23 2.7102749912763025E-4 0.0 0.0 6.775687478190756E-5 3.387843739095378E-4 24-25 2.7102749912763025E-4 0.0 0.0 2.032706243457227E-4 3.387843739095378E-4 26-27 2.7102749912763025E-4 0.0 0.0 3.3878437390953784E-4 3.387843739095378E-4 28-29 3.04905936518584E-4 0.0 0.0 0.0010163531217286133 3.387843739095378E-4 30-31 3.387843739095378E-4 0.0 0.0 0.0023714906173667645 3.387843739095378E-4 32-33 3.387843739095378E-4 0.0 0.0 0.0038960202999596847 3.387843739095378E-4 34-35 4.065412486914453E-4 0.0 0.0 0.005860969668635004 3.387843739095378E-4 36-37 4.742981234733529E-4 0.0 0.0 0.00833409559817463 3.387843739095378E-4 38-39 4.742981234733529E-4 0.0 0.0 0.012094602148570499 3.387843739095378E-4 40-41 4.742981234733529E-4 0.0 0.0 0.01731188150677738 3.387843739095378E-4 42-43 4.742981234733529E-4 0.0 0.0 0.024595745545832444 3.387843739095378E-4 44-45 4.742981234733529E-4 0.0 0.0 0.0318796095848875 3.387843739095378E-4 46-47 5.081765608643068E-4 0.0 0.0 0.04451626673171327 3.387843739095378E-4 48-49 5.420549982552605E-4 0.0 0.0 0.060473010742852495 3.387843739095378E-4 50-51 5.420549982552605E-4 0.0 0.0 0.06806178071842614 4.065412486914454E-4 52-53 5.759334356462142E-4 0.0 0.0 0.07900451599570421 4.742981234733529E-4 54-55 6.09811873037168E-4 0.0 0.0 0.09696008781290971 4.742981234733529E-4 56-57 6.09811873037168E-4 0.0 0.0 0.12677311271694905 4.742981234733529E-4 58-59 6.09811873037168E-4 0.0 0.0 0.1658688294661097 4.742981234733529E-4 60-61 6.775687478190756E-4 0.0 0.0 0.20977528432478582 5.081765608643068E-4 62-63 6.775687478190756E-4 0.0 0.0 0.2529702919982519 5.420549982552605E-4 64-65 6.775687478190756E-4 0.0 0.0 0.3013825790299248 5.420549982552605E-4 66-67 6.775687478190756E-4 0.0 0.0 0.3730015956744011 5.759334356462142E-4 68-69 6.775687478190756E-4 0.0 0.0 0.45580049665789213 6.09811873037168E-4 70-71 7.114471852100294E-4 0.0 0.0 0.5025866186947993 6.09811873037168E-4 72-73 7.453256226009832E-4 0.0 0.0 0.56654910848892 7.453256226009832E-4 74-75 7.453256226009832E-4 0.0 0.0 0.6305454767204317 8.808393721647982E-4 76-77 7.453256226009832E-4 0.0 0.0 0.7047731330440115 8.808393721647982E-4 78-79 7.453256226009832E-4 0.0 0.0 0.7965836983734962 8.808393721647982E-4 80-81 8.130824973828908E-4 0.0 0.0 0.9259993292069397 8.808393721647982E-4 82-83 8.808393721647982E-4 0.0 0.0 1.0688308212472009 9.485962469467058E-4 84-85 8.808393721647982E-4 0.0 0.0 1.280672690252835 9.485962469467058E-4 86-87 9.147178095557521E-4 0.0 0.0 1.5754489739915236 9.824746843376596E-4 88-89 9.485962469467058E-4 0.0 0.0 1.8987509020133957 0.0010163531217286133 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6395 0.0 25.04304 1 TACATGG 6440 0.0 24.94015 2 TACCTGG 1275 0.0 24.598124 2 GTACCTG 1400 0.0 24.440052 1 ACATGGG 6520 0.0 23.54011 3 GAGTACT 5425 0.0 21.859966 12-13 GTATCAA 11415 0.0 21.107157 1 CGTACAC 140 4.8405994E-4 20.364641 3 GTACTTT 6045 0.0 19.614594 14-15 GGTATCA 8870 0.0 19.287529 1 AGAGTAC 8910 0.0 19.205824 10-11 CATGGGA 3430 0.0 18.70222 4 AGTACTT 5860 0.0 18.493364 12-13 CAACGCA 12910 0.0 18.4408 5 TCAACGC 12930 0.0 18.41165 4 TGTACCG 155 9.6003216E-4 18.394491 5 ATCAACG 12950 0.0 18.346523 3 AACGCAG 13145 0.0 18.291872 6 TATCAAC 13205 0.0 18.136787 2 GTCTACG 135 0.009376224 17.60086 1 >>END_MODULE