FastQCFastQC Report
Thu 26 May 2016
SRR936668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475865
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65780.44570472231538794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56160.38052260877519284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51580.3494899601250792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31100.2107238805717325No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23850.16160014635484954No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20300.13754645580727234No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16350.11078249026841885No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.10603950903368532No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15630.1059039952841215No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14870.10075447280069655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG63100.024.0971891
TACATGG65250.022.9312972
GAGTACT65700.022.66117712-13
ACATGGG63500.022.5892773
GTACCTG14200.022.4198271
TACCTGG13650.021.9233262
GTACTTT70850.020.91341814-15
TACCGGG1250.00603153819.0002172
AGTACTT68450.018.90615812-13
CATGGGG29400.018.7385354
AATACCG1300.00757101618.2663425
GGTATCA121950.018.2351681
GTGTAGG6800.018.1681291
ACTTTTT81750.018.09530316-17
CCTACAC6100.017.9088273
AGAGTAC110200.017.66842510-11
GTCTACG1350.00938268117.5987571
GGACCGC1901.8698387E-417.4954576
GTGTAGC6800.017.4693551
GTCCTAC3601.899025E-917.1587891
Produced by FastQC (version 0.11.5)